Restoration of microRNA-130b expression suppresses osteosarcoma cell malignant behavior in vitro.

Abstract:

:The alteration of microRNA (miR)-130b expression has been associated with promoting or suppressing numerous types of human cancer. A previous study evaluated the expression level of miR-130b in osteosarcoma tissues, and subsequently investigated the effects of miR-130b on the regulation of osteosarcoma cells malignant behavior in vitro. The study revealed that miR-130b expression levels were significantly reduced in osteosarcoma tissues and cell lines, compared with in adjacent tissues or normal cell lines. The expression of miR-130b inhibited the proliferation of osteosarcoma U-2OS and Saos-2 cells and impaired their ability to migrate, invade and form colonies. Furthermore, analysis using TargetScan and a dual-luciferase reporter assay demonstrated that miR-130b directly interacted with the 3'-untranslated region of transforming growth factor α (TGFA) and suppressed TGFA expression. TGFA and miR-130b were also inversely expressed in osteosarcoma tissues. In addition, expression of TGFA was able to alter miR-130-regulated osteosarcoma cell proliferation, migration and invasion. Thus, the present study demonstrated that miR-130b was downregulated in osteosarcoma tissues and cell lines, whereas the expression of miR-130b suppressed osteosarcoma cell malignant behavior. At the gene level, miR-130 directly targets and inhibits TGFA expression, in addition to phosphorylated protein kinase B and epidermal growth factor receptor expression levels. Further study is required to evaluate miR-130b antitumor activity in osteosarcoma.

journal_name

Oncol Lett

journal_title

Oncology letters

authors

Wu Y,Sun W,Kong Y,Liu B,Zeng M,Wang W

doi

10.3892/ol.2018.8643

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

97-104

issue

1

eissn

1792-1074

issn

1792-1082

pii

OL-0-0-8643

journal_volume

16

pub_type

杂志文章
  • Diagnosis of hepatocellular carcinoma using a novel anti-glycocholic acid monoclonal antibody-based method.

    abstract::Glycocholic acid (GCA) is a novel identified biomarker for hepatocellular carcinoma (HCC). However, clinical pathological study of GCA has not been extensive due to the limited availability of anti-GCA monoclonal antibodies (mAbs) and restricted detection methods. In the present study, using human GCA conjugated with ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2019.9943

    authors: Wu M,Zhai S,Gao J,Wei D,Xue J,Zhou Y,Li N,Hu L

    更新日期:2019-03-01 00:00:00

  • Reduced expression of microRNA-139-5p in hepatocellular carcinoma results in a poor outcome: An exploration the roles of microRNA-139-5p in tumorigenesis, advancement and prognosis at the molecular biological level using an integrated meta-analysis and bi

    abstract::Hepatocellular carcinoma (HCC) is generally considered one of the most common gastrointestinal malignant tumors, characterized by high invasiveness and metastatic rate, as well as insidious onset. A relationship between carcinogenicity and aberrant microRNA-139-5p (miR-139-5p) expression has been identified in multipl...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2019.11031

    authors: Qin H,Wen DY,Que Q,Zhou CY,Wang XD,Peng YT,He Y,Yang H,Liao BM

    更新日期:2019-12-01 00:00:00

  • Prognostic significance of sarcopenia in patients with hepatocellular carcinoma undergoing sorafenib therapy.

    abstract::The present study aimed to examine the impact of sarcopenia, defined as low muscle mass on computed tomography (CT), prior to sorafenib therapy on the clinical outcomes of patients with hepatocellular carcinoma (HCC) receiving sorafenib therapy. In total, 232 patients with unresectable HCC (median age, 72 years) were ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.6287

    authors: Nishikawa H,Nishijima N,Enomoto H,Sakamoto A,Nasu A,Komekado H,Nishimura T,Kita R,Kimura T,Iijima H,Nishiguchi S,Osaki Y

    更新日期:2017-08-01 00:00:00

  • Modulation of G2/M cell cycle arrest and apoptosis by luteolin in human colon cancer cells and xenografts.

    abstract::The aim of the present study was to investigate the underlying molecular mechanisms of the potent cell cycle inhibition and apoptotic effect of luteolin on LoVo human colon cancer cells. In the present study, Cell Counting kit-8 assay revealed that luteolin exerted notable cytotoxicity on LoVo cells in a dose- and tim...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.7475

    authors: Chen Z,Zhang B,Gao F,Shi R

    更新日期:2018-02-01 00:00:00

  • Surgical wound management made easier and more cost-effective.

    abstract::Evidence-based guidelines for the prevention of surgical site infection (SSI) have been published by the U.S. Centers for Disease Control and Prevention (CDC). According to these guidelines, a wound should usually be covered with a sterile dressing for 24 to 48 h when a surgical incision is closed primarily. However, ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2012.687

    authors: Akagi I,Furukawa K,Miyashita M,Kiyama T,Matsuda A,Nomura T,Makino H,Hagiwara N,Takahashi K,Uchida E

    更新日期:2012-07-01 00:00:00

  • Activation of PGE2/EP2 and PGE2/EP4 signaling pathways positively regulate the level of PD-1 in infiltrating CD8+ T cells in patients with lung cancer.

    abstract::The present study aimed to identify the level of programmed death-1 (PD-1) expression in infiltrating cluster of differentiation (CD)4+ and CD8+ T cells isolated from lung cancer tissues, and investigated whether the level of PD-1 expression may be directly regulated by lung cancer cells via prostaglandin E2 (PGE2)-as...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.7279

    authors: Wang J,Zhang L,Kang D,Yang D,Tang Y

    更新日期:2018-01-01 00:00:00

  • The diagnostic and prognostic value of plasma microRNA-125b-5p in patients with multiple myeloma.

    abstract::Aberrant expression of microRNAs (miRNAs) contributes to the progression and outcomes of several types of tumor, while circulating miRNAs have been reported to act as biomarkers for several types of cancer. To identify specific circulating miRNAs associated with multiple myeloma (MM), a miRNA microarray analysis was u...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2018.9128

    authors: Jiang Y,Luan Y,Chang H,Chen G

    更新日期:2018-09-01 00:00:00

  • Ketamine suppresses the proliferation of rat C6 glioma cells.

    abstract::The present study investigated the effects of N-methyl-D-aspartate receptor (NMDAR) antagonist ketamine, on the growth of gliomas. To analyze the effects of ketamine treatment, rat C6 glioma cells arising from astrocytes, and RNB cells representing non-malignant astrocytes, were examined. In ketamine-treated C6 cells,...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.6806

    authors: Niwa H,Furukawa KI,Seya K,Hirota K

    更新日期:2017-10-01 00:00:00

  • Small cell carcinoma of the rectum: A report of imaging results from four cases.

    abstract::Small cell carcinoma of the rectum is extremely rare. The present study aimed to improve the recognition of computed tomography (CT) and magnetic resonance imaging (MRI) features observed in patients with small cell carcinoma of the rectum. A total of 4 cases of patients with histologically confirmed small cell carcin...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2016.4136

    authors: Zhou J,Shen J,Ma H,Zhang Y,Sun M,Zheng L,Lv Y

    更新日期:2016-03-01 00:00:00

  • Enhanced anticancer effect of oncostatin M combined with salinomycin in CD133+ HepG2 liver cancer cells.

    abstract::Oncostatin M (OSM) induces the differentiation of liver cancer stem cells (LCSCs) and increases sensitivity to the chemotherapeutic agent 5-fluorouracil, whereas salinomycin (Sal) induces apoptosis in cancer stem cells and inhibits the proliferation of liver cancer cells. However, there have been no studies investigat...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2018.9796

    authors: Fu C,Wang L,Tian G,Zhang C,Zhao Y,Xu H,Su M,Wang Y

    更新日期:2019-02-01 00:00:00

  • Identifying the differentially expressed microRNAs in esophagus squamous cell carcinoma of Kazakh patients in Xinjiang.

    abstract::Despite improvements in diagnosis and treatment, the survival of patients with advanced stages of esophageal squamous cell carcinoma (ESCC) remains poor. Therefore, novel biomarkers that can assist with early detection of ESCC are required. In the present study, three paired ESCC and normal esophageal tissue samples f...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2019.9904

    authors: Cheng L,Shi G,Fang C,Li G,Zheng Y,Chen W

    更新日期:2019-03-01 00:00:00

  • SET and MYND domain-containing protein 3 inhibits tumor cell sensitivity to cisplatin.

    abstract::Cisplatin resistance has been a major factor limiting its clinical use as a chemotherapy drug. The present study aimed to investigate whether SET and MYND domain-containing protein 3 (SMYD3), a histone methyltransferase closely associated with tumors can affect the sensitivity of tumors to cisplatin chemotherapy. Real...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11465

    authors: Wang L,Xu ML,Wang C,Dong QQ,Miao Z,Chen XY,Wang N,He HP,Zhang TC,Luo XG

    更新日期:2020-05-01 00:00:00

  • Targeted silencing of the ADP-ribosyltransferase 3 gene inhibits the migration ability of melanoma cells.

    abstract::Melanoma is the most common primary intraocular malignancy and metastasis of melanoma to other organs often results in a poor prognosis. ADP-ribosyltransferase 3 (ART3) is involved in cell division and DNA repair. However, its biological function in melanoma remains unclear. In the present study, it was identified tha...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2018.8252

    authors: He J,Li Y,Wang Y,Zhang H,Ge S,Fan X

    更新日期:2018-05-01 00:00:00

  • Expression of microRNA-30a-5p in drug-resistant and drug-sensitive ovarian cancer cell lines.

    abstract::The present study aimed to explore the expression of microRNA (miRNA or miR) in drug-resistant and drug-sensitive ovarian cancer cell lines, and to seek the potential therapeutic target of ovarian cancer drug-resistant mechanism in order to improve drug resistance by altering miRNA levels. The drug-resistant character...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2016.4831

    authors: Liu J,Wu X,Liu H,Liang Y,Gao X,Cai Z,Wang W,Zhang H

    更新日期:2016-09-01 00:00:00

  • α-smooth muscle actin-positive fibroblasts correlate with poor survival in hepatocellular carcinoma.

    abstract::The current case-control study was conducted to assess the value of activated fibroblasts with high α-smooth muscle actin (α-SMA) expression as an indicator of survival in hepatocellular carcinoma (HCC) patients. In total, 47 patients diagnosed with HCC who underwent a liver biopsy at the Veterans Affairs Medical Cent...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1720

    authors: Parikh JG,Kulkarni A,Johns C

    更新日期:2014-02-01 00:00:00

  • Evaluation of the improved tubeless cutaneous ureterostomy technique following radical cystectomy in cases of invasive bladder cancer complicated by peritoneal metastasis.

    abstract::Radical cystectomy, as the most common surgical treatment for patients with invasive bladder cancer (IBC) complicated by peritoneal metastasis, is usually accompanied by a urinary diversion procedure. In this study, we evaluated the improved tubeless cutaneous ureterostomy technique by comparing the resulting clinical...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2015.4045

    authors: Liu Z,Tian Q,Xia S,Yin H,Yao D,Xiu Y

    更新日期:2016-02-01 00:00:00

  • A prognosis-predictive nomogram of ovarian cancer with two immune-related genes: CDC20B and PNPLA5.

    abstract::Ovarian carcinoma (OV) is one of the most lethal gynecological malignancies globally, and the overall 5-year survival rate of OV was 47% in 2018 according to American data. To increase the survival rate of patients with OV, many researchers have sought to identify biomarkers that act as both prognosis-predictive marke...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.12067

    authors: Lin H,Wang J,Wen X,Wen Q,Huang S,Mai Z,Lu L,Liang X,Pan H,Li S,He Y,Ma H

    更新日期:2020-11-01 00:00:00

  • Potassium increases the antitumor effects of ascorbic acid in breast cancer cell lines in vitro.

    abstract::Ascorbic acid (A) has been demonstrated to exhibit anti-cancer activity in association with chemotherapeutic agents. Potassium (K) is a regulator of cellular proliferation. In the present study, the biological effects of A and K bicarbonate, alone or in combination (A+K), on breast cancer cell lines were evaluated. Th...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2016.4506

    authors: Frajese GV,Benvenuto M,Fantini M,Ambrosin E,Sacchetti P,Masuelli L,Giganti MG,Modesti A,Bei R

    更新日期:2016-06-01 00:00:00

  • Circulating tumor cell detection: A direct comparison between negative and unbiased enrichment in lung cancer.

    abstract::Circulating tumor cells (CTCs), isolated as a 'liquid biopsy', may provide important diagnostic and prognostic information. Therefore, rapid, reliable and unbiased detection of CTCs are required for routine clinical analyses. It was demonstrated that negative enrichment, an epithelial marker-independent technique for ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.6046

    authors: Xu Y,Liu B,Ding F,Zhou X,Tu P,Yu B,He Y,Huang P

    更新日期:2017-06-01 00:00:00

  • Clinical outcomes of giant cell tumor of bone treated with bone cement filling and internal fixation, and oral bisphosphonates.

    abstract::Giant cell tumor (GCT) of the bone is a relatively common primary bone tumor. Treatment with simple curettage often results in a high local recurrence rate. Tumor resection and reconstruction with prosthesis or an allograft has a low rate of local recurrence; however, the patient's native joint function becomes signif...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2012.1036

    authors: Yu X,Xu M,Xu S,Su Q

    更新日期:2013-02-01 00:00:00

  • Mutations of the TERT promoter are associated with aggressiveness and recurrence/distant metastasis of papillary thyroid carcinoma.

    abstract::Several previous studies have shown that mutations in B-Raf proto-oncogene (BRAF) and telomerase reverse transcriptase (TERT) can be used for the diagnosis and prognosis of papillary thyroid carcinoma (PTC). However, whether mutations in BRAF and the TERT promoter may improve the accurate identification and risk strat...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11904

    authors: Liu R,Li Y,Chen W,Cong J,Zhang Z,Ma L,Chu L,Xiao H,Zhang Y,Liu Y,Xu Y,Yu Q,Yang X,Sun C

    更新日期:2020-10-01 00:00:00

  • Imbalanced adaptive responses associated with microsatellite instability in cholangiocarcinoma.

    abstract::The adaptive response of the genome protection mechanism occurs in cells when exposed to genotoxic stress due to the overproduction of free radicals via inflammation and infection. In such circumstances, cells attempt to maintain health via several genome protection mechanisms. However, evidence is increasing that thi...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2016.5477

    authors: Loilome W,Kadsanit S,Muisook K,Yongvanit P,Namwat N,Techasen A,Puapairoj A,Khuntikeo N,Phonjit P

    更新日期:2017-02-01 00:00:00

  • Molecular pathological predictive diagnostics in a patient with non-small cell lung cancer treated with crizotinib therapy: A case report.

    abstract::Lung cancer is one of the most common malignant cancers in the Czech Republic in men, with the highest mortality rate of all the malignant diseases. The development of biological treatment enables study into novel personalized treatment options. This type of treatment is usually of high quality, and is often demanding...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.7167

    authors: Stanek L,Springer D,Konopasek B,Vocka M,Tesarova P,Syrucek M,Petruzelka L,Vicha A,Musil Z

    更新日期:2017-12-01 00:00:00

  • miR-331-3p suppresses cell invasion and migration in colorectal carcinoma by directly targeting NRP2.

    abstract::Colorectal carcinoma (CRC) is a common tumor of the digestive system with poor prognosis. Studies have shown that aberrant microRNA (miRNA) expression can affect CRC progression by regulating target genes. In the present study, we investigated the functional roles and potential mechanisms of miR-331-3p in CRC. The exp...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2019.11029

    authors: Zhang H,Wang R,Wang M

    更新日期:2019-12-01 00:00:00

  • Effects and mechanism of the bile acid (farnesoid X) receptor on the Wnt/β-catenin signaling pathway in colon cancer.

    abstract::The downregulation of farnesoid X receptor (FXR; gene name, nuclear receptor subfamily 1 group h member 4), an enteric nuclear bile acid receptor, has been reported in colorectal carcinoma (CRC), and FXR expression has been inversely correlated with CRC stage and clinical outcome. FXR knockdown in chronic colitis mous...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11545

    authors: Mao J,Chen X,Wang C,Li W,Li J

    更新日期:2020-07-01 00:00:00

  • Combining angioplasty with percutaneous microwave ablation for treating primary Budd-Chiari syndrome associated with hepatocellular carcinoma in two patients: A case report.

    abstract::Percutaneous transluminal angioplasty using balloon catheters for Budd-Chiari syndrome (BCS) and transcatheter arterial chemoembolization (TACE) for unresectable hepatocellular carcinoma (HCC) have become increasingly accepted as alternative therapeutic modalities. However, few studies have investigated the clinical e...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1417

    authors: Zhang QQ,Zu MH,Xu H,Gu YM,Wang WL,Gao ZK

    更新日期:2013-08-01 00:00:00

  • BRCA1 regulates insulin-like growth factor 1 receptor levels in ovarian cancer.

    abstract::Breast cancer 1 (BRCA1) and insulin-like growth factor 1 receptor (IGF1R) are critical in ovarian cancer progression. However, the crosstalk between the BRCA1 and IGF1R signaling pathways in ovarian cancer remains largely unknown. The effects of BRCA1 on IGF1R were assessed in 121 serous ovarian cancer patients (BRCA1...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2014.1929

    authors: Liu B,Li D,Guan YF

    更新日期:2014-05-01 00:00:00

  • Colon cancer metastasis to mediastinal lymph nodes without liver or lung involvement: A case report.

    abstract::Colon cancer is the second most common type of cancer in females and the third in males, worldwide. The most common sites of colon cancer metastasis are the regional lymph nodes, liver, lung, bone and brain. In this study, an extremely rare case of colon adenocarcinoma with extensive metastasis to the mediastinal lymp...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2014.2426

    authors: El-Halabi MM,Chaaban SA,Meouchy J,Page S,Salyers WJ Jr

    更新日期:2014-11-01 00:00:00

  • New role of human ribosomal protein S3: Regulation of cell cycle via phosphorylation by cyclin-dependent kinase 2.

    abstract::Human ribosomal protein S3 (hRpS3) is a component of the 40S ribosomal subunit that associated in protein synthesis. hRpS3 has additional ribosomal functions such as DNA repair, transcription, metastasis, and apoptosis via interaction with numerous signaling molecules and has different modifications. Cyclin-dependent ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.5906

    authors: Han SH,Chung JH,Kim J,Kim KS,Han YS

    更新日期:2017-05-01 00:00:00

  • Expression of c-Met and hepatocyte growth factor in various gastric pathologies and its association with Helicobacter pylori infection.

    abstract::Activation of the hepatocyte growth factor (HGF)/c-Met signaling pathway was identified to be associated with malignant tumors. The present study aimed at determining the expression of HGF and c-Met in gastric carcinogenesis and its association with Helicobacter pylori infection. Gastric biopsies were obtained from 16...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.6983

    authors: Xie C,Yang Z,Hu Y,Cao X,Chen J,Zhu Y,Lu N

    更新日期:2017-11-01 00:00:00