Abstract:
:The inherent specificity and electrochemical reversibility of enzymes poise them as the biorecognition element of choice for a wide range of metabolites. To use enzymes efficiently in biosensors, the redox centers of the protein should have good electrical communication with the transducing electrode, which requires either the use of mediators or tedious biofunctionalization approaches. We report an all-polymer micrometer-scale transistor platform for the detection of lactate, a significant metabolite in cellular metabolic pathways associated with critical health care conditions. The device embodies a new concept in metabolite sensing where we take advantage of the ion-to-electron transducing qualities of an electron-transporting (n-type) organic semiconductor and the inherent amplification properties of an ion-to-electron converting device, the organic electrochemical transistor. The n-type polymer incorporates hydrophilic side chains to enhance ion transport/injection, as well as to facilitate enzyme conjugation. The material is capable of accepting electrons of the enzymatic reaction and acts as a series of redox centers capable of switching between the neutral and reduced state. The result is a fast, selective, and sensitive metabolite sensor. The advantage of this device compared to traditional amperometric sensors is the amplification of the input signal endowed by the electrochemical transistor circuit and the design simplicity obviating the need for a reference electrode. The combination of redox enzymes and electron-transporting polymers will open up an avenue not only for the field of biosensors but also for the development of enzyme-based electrocatalytic energy generation/storage devices.
journal_name
Sci Advjournal_title
Science advancesauthors
Pappa AM,Ohayon D,Giovannitti A,Maria IP,Savva A,Uguz I,Rivnay J,McCulloch I,Owens RM,Inal Sdoi
10.1126/sciadv.aat0911subject
Has Abstractpub_date
2018-06-22 00:00:00pages
eaat0911issue
6issn
2375-2548pii
aat0911journal_volume
4pub_type
杂志文章相关文献
Science Advances文献大全abstract::By introducing a superconducting gap in Weyl or Dirac semimetals, the superconducting state inherits the nontrivial topology of their electronic structure. As a result, Weyl superconductors are expected to host exotic phenomena, such as nonzero-momentum pairing due to their chiral node structure, or zero-energy Majora...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1602983
更新日期:2017-05-24 00:00:00
abstract::B cell activation is regulated by the stimulatory or inhibitory co-receptors of B cell receptors (BCRs). Here, we investigated the signaling mechanism of Fc receptor-like 1 (FcRL1), a newly identified BCR co-receptor. FcRL1 was passively recruited into B cell immunological synapses upon BCR engagement in the absence o...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaw0315
更新日期:2019-07-17 00:00:00
abstract::Metal-organic frameworks (MOFs) have been attracting intensive attention because of their commendable potential in many applications. Postsynthetic modification for redesigning chemical characteristics and pore structures can greatly improve performance and expand functionality of MOF materials. Here, we develop a ver...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax7270
更新日期:2020-05-01 00:00:00
abstract::Mycobacterium tuberculosis (Mtb) infection results in a spectrum of clinical and histopathologic manifestations. It has been proposed that the environmental and immune pressures associated with different contexts of infection have different consequences for the associated bacterial populations, affecting drug suscepti...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aba4901
更新日期:2020-05-29 00:00:00
abstract::The cyclic GMP-AMP synthase (cGAS), a sensor of cytosolic DNA, is critical for the innate immune response. Here, we show that loss of cGAS in untransformed and cancer cells results in uncontrolled DNA replication, hyperproliferation, and genomic instability. While the majority of cGAS is cytoplasmic, a fraction of cGA...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abb8941
更新日期:2020-10-14 00:00:00
abstract::An understanding of the normal state in the high-temperature superconducting cuprates is crucial to the ultimate understanding of the long-standing problem of the origin of the superconductivity itself. This so-called "strange metal" state is thought to be associated with a quantum critical point (QCP) hidden beneath ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aav6753
更新日期:2019-05-17 00:00:00
abstract::The Paris Agreement aims to mitigate the potential impacts of climate change on ecological and social systems. Using an ensemble of climate-marine ecosystem and economic models, we explore the effects of implementing the Agreement on fish, fishers, and seafood consumers worldwide. We find that implementing the Agreeme...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau3855
更新日期:2019-02-27 00:00:00
abstract::Extinction of conditioned fear necessitates the dynamic involvement of hippocampus, medial prefrontal cortex (mPFC), and basolateral amygdala (BLA), but key molecular players that regulate these circuits to achieve fear extinction remain largely unknown. Here, we report that acid-sensing ion channel 1a (ASIC1a) is a c...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau3075
更新日期:2018-10-24 00:00:00
abstract::Compound climate extremes are receiving increasing attention because of their disproportionate impacts on humans and ecosystems. However, risks assessments generally focus on univariate statistics. We analyze the co-occurrence of hot and dry summers and show that these are correlated, inducing a much higher frequency ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1700263
更新日期:2017-06-28 00:00:00
abstract::Cellular cross-talk between ubiquitination and other posttranslational modifications contributes to the regulation of numerous processes. One example is ADP-ribosylation of the carboxyl terminus of ubiquitin by the E3 DTX3L/ADP-ribosyltransferase PARP9 heterodimer, but the mechanism remains elusive. Here, we show that...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abc0418
更新日期:2020-09-18 00:00:00
abstract::The key component currently missing for the next generation of transparent and flexible displays is a high-performance polymer material that is flexible, while showing optical and thermal properties of glass. It must be transparent to visible light and show a low coefficient of thermal expansion (CTE). While specialty...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aau1956
更新日期:2018-10-26 00:00:00
abstract::Deep CO2 emissions characterize many nonvolcanic, seismically active regions worldwide, and the involvement of deep CO2 in the earthquake cycle is now generally recognized. However, no long-time records of such emissions have been published, and the temporal relations between earthquake occurrence and tectonic CO2 rel...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abc2938
更新日期:2020-08-26 00:00:00
abstract::Metabolic reprogramming is a major factor in transformation, and particular metabolic phenotypes correlate with oncogenotype, tumor progression, and metastasis. By profiling metabolites in 17 patient-derived xenograft melanoma models, we identified durable metabolomic signatures that correlate with biological features...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aao5268
更新日期:2017-11-01 00:00:00
abstract::We have positionally cloned the Ym1 gene, with a duplication and a promoter polymorphism, as a major regulator of inflammation. Mice with the RIIIS/J haplotype, with the absence of Ym1 expression, showed reduced susceptibility to mannan-enhanced collagen antibody-induced arthritis and to chronic arthritis induced by i...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aba9337
更新日期:2020-10-21 00:00:00
abstract::Deforestation is the primary driver of carbon losses in tropical forests, but it does not operate alone. Forest fragmentation, a resulting feature of the deforestation process, promotes indirect carbon losses induced by edge effect. This process is not implicitly considered by policies for reducing carbon emissions in...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz8360
更新日期:2020-09-30 00:00:00
abstract::Goat domestication was critical for agriculture and civilization, but its underlying genetic changes and selection regimes remain unclear. Here, we analyze the genomes of worldwide domestic goats, wild caprid species, and historical remains, providing evidence of an ancient introgression event from a West Caucasian tu...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz5216
更新日期:2020-05-20 00:00:00
abstract::Ewing sarcoma (EwS) is an aggressive tumor that affects adolescents and young adults. EwS is defined by a chromosomal translocation, EWSR1-FLI1 being the most common, that causes genome reprogramming through remodeling of enhancers. Here, we describe an unexpected function of RING1B, which is highly expressed in EwS. ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aba3058
更新日期:2020-10-23 00:00:00
abstract::Homologous recombination is exquisitely activated only during specific cell phases. In the G1 phase, homologous recombination activity is completely suppressed. According to previous reports, the activation of homologous recombination during specific cell phases depends on the kinase activity of cyclin-dependent kinas...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aay2669
更新日期:2020-02-07 00:00:00
abstract::We present results from a study in the Democratic Republic of the Congo that uses mobile money networks to run rotating savings and credit associations (ROSCAs), peer-to-peer finance groups ubiquitous across the developing world. We find high rates of contribution and ROSCA success. The unexpected success of such e-RO...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abc5831
更新日期:2021-01-01 00:00:00
abstract::Engineered surfaces with reversibly switching interfacial properties, such as wettability and liquid repellency, are highly desirable in diverse application fields but are rare. We have developed a general concept to prepare metallic porous surfaces with exceptionally powerful wettability switch capabilities and liqui...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax0380
更新日期:2019-11-01 00:00:00
abstract::Ornithine transcarbamylase (OTC) deficiency is an X-linked urea cycle disorder associated with high mortality. Although a promising treatment for late-onset OTC deficiency, adeno-associated virus (AAV) neonatal gene therapy would only provide short-term therapeutic effects as the non-integrated genome gets lost during...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax5701
更新日期:2020-02-12 00:00:00
abstract::Implanted brain electrodes construct the only means to electrically interface with individual neurons in vivo, but their recording efficacy and biocompatibility pose limitations on scientific and clinical applications. We showed that nanoelectronic thread (NET) electrodes with subcellular dimensions, ultraflexibility,...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.1601966
更新日期:2017-02-15 00:00:00
abstract::The response to programmed cell death protein-1 (PD-1)/programmed death ligand-1 (PD-L1) blockade in cancer immunotherapy is limited because of multiple immune evasion mechanisms. Here, a previously unknown strategy is proposed to synergize the nuclear factor κB (NF-κB) inhibition and PD-1 blockade for antitumor immun...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aay7785
更新日期:2020-02-05 00:00:00
abstract::Donkeys and horses share a common ancestor dating back to about 4 million years ago. Although a high-quality genome assembly at the chromosomal level is available for the horse, current assemblies available for the donkey are limited to moderately sized scaffolds. The absence of a better-quality assembly for the donke...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaq0392
更新日期:2018-04-04 00:00:00
abstract::The homotrimeric molecular chaperone Skp of Gram-negative bacteria facilitates the transport of outer membrane proteins across the periplasm. It has been unclear how its activity is modulated during its functional cycle. Here, we report an atomic-resolution characterization of the Escherichia coli Skp monomer-trimer t...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.abc5822
更新日期:2020-10-21 00:00:00
abstract::The past few years have witnessed rapid advances in the synthesis of high-quality perovskite nanocrystals (PNCs). However, despite the impressive developments, the stability of PNCs remains a substantial challenge. The ability to reliably improve stability of PNCs while retaining their individual nanometer size repres...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aax4424
更新日期:2019-11-29 00:00:00
abstract::Nanometer-scale skyrmions are prospective candidates for information bits in low-power consumption devices owing to their topological nature and controllability with low current density. Studies on skyrmion dynamics in different classes of materials have exploited the topological Hall effect and current-driven fast mo...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz9744
更新日期:2020-06-17 00:00:00
abstract::The integration of structure and function for tissue engineering scaffolds is of great importance in mimicking native bone tissue. However, the complexity of hierarchical structures, the requirement for mechanical properties, and the diversity of bone resident cells are the major challenges in constructing biomimetic ...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaz6725
更新日期:2020-03-20 00:00:00
abstract::Human settlement of the Caribbean represents the only example in the Americas of peoples colonizing islands that were not visible from surrounding mainland areas or other islands. Unfortunately, many interpretive models have relied on radiocarbon determinations that do not meet standard criteria for reporting because ...
journal_title:Science advances
pub_type: 历史文章,杂志文章
doi:10.1126/sciadv.aar7806
更新日期:2019-12-18 00:00:00
abstract::The investigation of materials that exhibit quantum phase transition provides valuable insights into fundamental problems in physics. We present neutron scattering under pressure in a triangular-lattice antiferromagnet that has a quantum disorder in the low-pressure phase and a noncollinear structure in the high-press...
journal_title:Science advances
pub_type: 杂志文章
doi:10.1126/sciadv.aaw5639
更新日期:2019-10-18 00:00:00