Abstract:
:The aim of this study was to evaluate the feasibility and efficiency of using a sucrose gradient-based warming protocol as a universal warming approach on human cleavage stage embryos. Between January 2013 and November 2014, a total of 118 warming cycles were performed on 705 embryos which had previously been cryopreserved/thawed by slow freezing protocols or cryopreserved by slow freezing and warmed by vitrification thaw solution. Clinical outcomes have been retrospectively analyzed depending on cryopreservation and warming techniques used, embryo viability, day of cryopreservation, clinical pregnancy, implantation, and live birth rate. Results indicate that, the use of the vitrification warming protocol for warming after slow freezing results in comparable post-warming survival (71.6% and 71.1%; p = 0.890). Higher clinical pregnancy, implantation, and live birth rates were obtained in the cryopreserved embryos by slow freezing and warmed by vitrification group in comparison to the cryopreserved/thawed by slow freezing protocols group but the results did not show statistically significant differences between groups (p > 0.05). These results indicate that such an approach can eliminate the need to search for a brand-dependent product, as well as case-dependent hands-on planning. Further research that evaluates the effectiveness of this approach on a larger case series is underway. Abbreviations: CPA: concentrated cryoprotective agent; COH: controlled ovarian stimulation; FET: frozen embryo transfer; HSG: hysterosalpingogram; mHTF: modified human tubal medium; SSM: single step media; SSS: synthetic serum substitute; TV-USG: transvaginal ultrasound.
journal_name
Syst Biol Reprod Medjournal_title
Systems biology in reproductive medicineauthors
Serdarogullari M,Coban O,Boynukalin FK,Bilgin EM,Findikli N,Bahceci Mdoi
10.1080/19396368.2018.1487477subject
Has Abstractpub_date
2019-02-01 00:00:00pages
12-19issue
1eissn
1939-6368issn
1939-6376journal_volume
65pub_type
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journal_title:Systems biology in reproductive medicine
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journal_title:Systems biology in reproductive medicine
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journal_title:Systems biology in reproductive medicine
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journal_title:Systems biology in reproductive medicine
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journal_title:Systems biology in reproductive medicine
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journal_title:Systems biology in reproductive medicine
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journal_title:Systems biology in reproductive medicine
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journal_title:Systems biology in reproductive medicine
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