Regulation of the epidermal growth factor receptor by phosphorylation.

Abstract:

:The receptor for epidermal growth factor (EGF) is a glycosylated transmembrane phosphoprotein that exhibits EGF-stimulable protein tyrosine kinase activity. On EGF stimulation, the receptor undergoes a self-phosphorylation reaction at tyrosine residues located primarily in the extreme carboxyl-terminal region of the protein. Using enzymatically active EGF receptor purified by immunoaffinity chromatography from A431 human epidermoid carcinoma cells, the self-phosphorylation reaction has been characterized as a rapid, intramolecular process which is maximal at 30-37 degrees C and exhibits a very low Km for ATP (0.2 microM). When phosphorylation of exogenous peptide substrates was measured as a function of receptor self-phosphorylation, tyrosine kinase activity was found to be enhanced two to threefold at 1-2 mol of phosphate per mol of receptor. Analysis of the dependence of the tyrosine kinase activity on ATP concentration yielded hyperbolic kinetics when plotted in double-reciprocal fashion, indicating that ATP can serve as an activator of the enzyme. Higher concentrations of peptide substrates were found to inhibit both the self- and peptide phosphorylation, but this inhibition could be overcome by first self-phosphorylating the enzyme. These results suggest that self-phosphorylation can remove a competitive/inhibitory constraint so that certain exogenous substrates can have greater access to the enzyme active site. In addition to self-phosphorylation, the EGF receptor can be phosphorylated on threonine residues by the calcium- and phospholipid-dependent protein kinase C. The sites on the EGF receptor phosphorylated in vitro by protein kinase C are identical to the sites phosphorylated on the receptor isolated from A431 cells exposed to the tumor promoters 12-O-tetradecanoylphorbol 13-acetate or teleocidin. This phosphorylation of the EGF receptor results in a suppression of its tyrosine kinase and EGF binding activities both in vivo and in vitro. The EGF receptor can thus be variably regulated by phosphorylation: self-phosphorylation can enhance tyrosine kinase activity whereas protein kinase C-catalyzed phosphorylation can depress enzyme activity. Because these two phosphorylations account for only a fraction of the phosphate present in the EGF receptor in vivo, other protein kinases can apparently phosphorylate the receptor and these may exert additional controls on EGF receptor/kinase function.

journal_name

J Cell Biochem

authors

Bertics PJ,Weber W,Cochet C,Gill GN

doi

10.1002/jcb.240290304

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

195-208

issue

3

eissn

0730-2312

issn

1097-4644

journal_volume

29

pub_type

杂志文章
  • Effects of vitamin D3 on signaling by prostaglandin E2 in osteoblast-like cells.

    abstract::We investigated the effects of vitamin D3 on the signaling pathways by prostaglandin E2 (PGE2) in osteoblast-like MC3T3-E1 cells. The pretreatment with 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), an active form of vitamin D3, significantly inhibited cAMP accumulation induced by 10 microM PGE2 in a dose-dependent manner i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240520213

    authors: Tokuda H,Kotoyori J,Suzuki A,Oiso Y,Kozawa O

    更新日期:1993-06-01 00:00:00

  • Butyrate stimulates ApoA-IV-containing lipoprotein secretion in differentiated Caco-2 cells: role in cholesterol efflux.

    abstract::The aim of this study was to determine: (1) whether the Short Chain Fatty Acids (SCFA) Acetate, Propionate, and Butyrate enhance the synthesis and secretion of intestinal apolipoprotein A-IV-containing lipoproteins and (2) if so, whether these particles are able to promote cholesterol efflux in vitro. For this purpose...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1221

    authors: Nazih H,Nazih-Sanderson F,Krempf M,Michel Huvelin J,Mercier S,Marie Bard J

    更新日期:2001-08-01 00:00:00

  • Network analysis of icb-1 gene function in human breast cancer cells.

    abstract::Icb-1 is a human gene previously described by our group to exert important functions in cancer cells of different origin. We now performed microarray-based gene expression profiling with subsequent network modeling to further elucidate the role of icb-1 in breast cancer cells. Analyzing the effect of icb-1 knockdown o...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24175

    authors: Treeck O,Belgutay D,Häring J,Schüler S,Lattrich C,Ortmann O

    更新日期:2012-09-01 00:00:00

  • Analysis of synonymous codon usage pattern of genes in unique non-blood-sucking leech Whitmania pigra.

    abstract::Whitmania pigra is a unique, fluid-sucking ectoparasite and an anticoagulant medical leech. The codon usage bias (CUB) is the nonuniform usage of synonymous codons in which some codons are more preferred than others. Here, we performed a comprehensive analysis of CUB of genes in W. pigra, analyzing 140 780 transcripts...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28267

    authors: Khan MS,Guan DL,Ma LB,Xie JY,Xu SQ

    更新日期:2019-06-01 00:00:00

  • MiR-218 regulated cardiomyocyte differentiation and migration in mouse embryonic stem cells by targeting PDGFRα.

    abstract::MicroRNAs (miRNAs) have been identified as key players in cardiogenesis and heart pathophysiological processes. However, many miRNAs are still not recognized for their roles in cardiomyocytes differentiation. In this study, we evaluated the effects of microRNA-218 (miR-218) in cardiomyocyte differentiation of the mous...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27721

    authors: Xu T,Liu N,Shao Y,Huang Y,Zhu D

    更新日期:2019-03-01 00:00:00

  • Effect of homocysteinylation of low density lipoproteins on lipid peroxidation of human endothelial cells.

    abstract::Homocysteine-thiolactone (HcyT) is a toxic product whose synthesis is directly proportional to plasma homocysteine (Hcy) levels. Previous studies demonstrated that the interaction between HcyT and low density lipoproteins (LDL) induces the formation of homocystamide-LDL adducts (Hcy-LDL). Structural and functional alt...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20069

    authors: Ferretti G,Bacchetti T,Moroni C,Vignini A,Nanetti L,Curatola G

    更新日期:2004-05-15 00:00:00

  • Fullerene-polyvinylpyrrolidone clathrate localizes in the cytoplasm to prevent Ultraviolet-A ray-induced DNA-fragmentation and activation of the transcriptional factor NF-kappaB.

    abstract::By Western blot and immunostaining we proved that polyvinylpyrrolidone (PVP)-wrapped fullerene molecules (PVP-fullerene) could combine the 8- and 53-kb proteins which localize in the membrane of human skin keratinocytes HaCaT. Only fullerene molecules are able to cross the lipid membrane and conjugate 53-kb proteins i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22784

    authors: Xiao L,Aoshima H,Saitoh Y,Miwa N

    更新日期:2010-11-01 00:00:00

  • NERF2, a member of the Ets family of transcription factors, is increased in response to hypoxia and angiopoietin-1: a potential mechanism for Tie2 regulation during hypoxia.

    abstract::Vascular endothelial growth factor (VEGF) and angiopoietins regulate endothelial cell survival and migration and are essential for angiogenesis. Considerable progress has been made towards understanding hypoxia-mediated regulation of VEGF and its receptors. In contrast, little is known about the regulation of angiopoi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10148

    authors: Christensen RA,Fujikawa K,Madore R,Oettgen P,Varticovski L

    更新日期:2002-01-01 00:00:00

  • Importance of autophosphorylation at Ser186 in the A-loop of salt inducible kinase 1 for its sustained kinase activity.

    abstract::Autophosphorylation is an important mechanism by which protein kinases regulate their own biological activities. Salt inducible kinase 1 (SIK1) is a regulator in the feedback cascades of cAMP-mediated gene expression, while its kinase domain also features autophosphorylation activity. We provide evidence that Ser186 i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21737

    authors: Hashimoto YK,Satoh T,Okamoto M,Takemori H

    更新日期:2008-08-01 00:00:00

  • Ebselen impairs cellular oxidative state and induces endoplasmic reticulum stress and activation of crucial mitogen-activated protein kinases in pancreatic tumour AR42J cells.

    abstract::Ebselen (2-phenyl-1,2-benzisoselenazol-3(2H)-one) is an organoselenium radical scavenger compound, which has strong antioxidant and anti-inflammatory effects. However, evidence suggests that this compound could exert deleterious actions on cell physiology. In this study, we have analyzed the effect of ebselen on rat p...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26280

    authors: Santofimia-Castaño P,Izquierdo-Alvarez A,Plaza-Davila M,Martinez-Ruiz A,Fernandez-Bermejo M,Mateos-Rodriguez JM,Salido GM,Gonzalez A

    更新日期:2018-01-01 00:00:00

  • Clinical aspects of circulating miRNA-335 in breast cancer patients: A prospective study.

    abstract:BACKGROUND:The impact of aberrant expression of miRNAs, small noncoding RNAs of 19 to 23 nucleotide, has been reported in different types of cancer, such as breast cancer. Authors aim to investigate the role of circulating miRNA-335 as a diagnostic and prognostic marker for breast cancer. MATERIALS AND METHODS:miRNA-3...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28168

    authors: Swellam M,Mahmoud MS,Hashim M,Hassan NM,Sobeih ME,Nageeb AM

    更新日期:2019-06-01 00:00:00

  • Long noncoding RNA PAGBC contributes to nitric oxide (NO) production by sponging miR-511 in airway hyperresponsiveness upon intubation.

    abstract:BACKGROUND AND OBJECTIVES:In this study, we aimed to study the molecular mechanisms underlying the symptoms of hyperresponsiveness during intubation. METHOD:The value of circulating long noncoding RNA (lncRNA)-prognosis-associated gallbladder cancer (PAGBC) in the prediction of hyperresponsiveness upon intubation duri...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27513

    authors: Li LX,Li YJ,He JX

    更新日期:2018-09-23 00:00:00

  • The Leukemia Inhibitory Factor Receptor Gene Is a Direct Target of RUNX1.

    abstract::Activation of cytokine signaling via the leukemia inhibitory factor receptor (LIFR) plays an integral role in hematopoiesis, osteogenesis, and placental development, along with mediating neurotrophic mechanisms. However, the regulatory control of the LIFR gene has remained largely unexplored. Here, we characterize the...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25246

    authors: Qadi AA,Taberlay PC,Phillips JL,Young A,West AC,Brettingham-Moore KH,Dickinson JL,Holloway AF

    更新日期:2016-01-01 00:00:00

  • PI3K is involved in L-selectin- and PSGL-1-mediated neutrophil rolling on E-selectin via F-actin redistribution and assembly.

    abstract::L-selectin and P-selectin glycoprotein ligand-1 (PSGL-1) are adhesion molecules that play critical roles in neutrophil rolling during inflammation and lymphocyte homing. On the other hand they also function as signaling receptors to induce cytoskeleton changes. The present study is to investigate the signaling kinases...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22603

    authors: Luo J,Xu T,Wang X,Ba X,Feng X,Deepak V,Zeng X

    更新日期:2010-07-01 00:00:00

  • Retinoblastoma protein complexes with C/EBP proteins and activates C/EBP-mediated transcription.

    abstract::The retinoblastoma protein (RB) recruits histone deacetylase (HDAC) to repress E2F-mediated transactivation that plays a critical role in cell cycle regulation. RB is also involved in activation of expression of a number of tissue specific- and differentiation-related genes. In this study, we examined the mechanism by...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1239

    authors: Charles A,Tang X,Crouch E,Brody JS,Xiao ZX

    更新日期:2001-08-21 00:00:00

  • Ultraviolet radiation (UVR) activates p38 MAP kinase and induces post-transcriptional stabilization of the C/EBPdelta mRNA in G0 growth arrested mammary epithelial cells.

    abstract::The G(0) growth arrest (quiescent) state is highly conserved in evolution to promote survival under adverse environmental conditions. To maintain viability, G(0) growth arrested cells limit gene expression to essential growth control and pro-survival genes. CCAAT enhancer binding protein delta (C/EBPdelta), a member o...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21554

    authors: Li B,Si J,DeWille JW

    更新日期:2008-04-01 00:00:00

  • Interaction of the retinoblastoma gene product, RB, with cyclophilin A negatively affects cyclosporin-inhibited NFAT signaling.

    abstract::The retinoblastoma susceptibility gene product, p105Rb (RB), is generally believed to be an important regulator in the control of cell growth, differentiation, and apoptosis. Several cellular factors that form complexes with RB and exert their cellular regulatory functions have been identified, such as the newly ident...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10253

    authors: Cui Y,Mirkia K,Florence Fu YH,Zhu L,Yokoyama KK,Chiu R

    更新日期:2002-01-01 00:00:00

  • Calcium-dependent and calcium-independent adhesive mechanisms are present during initial binding events of neural retina cells.

    abstract::The hypothesis that intercellular adhesion can be subdivided into two separable phenomena, an initial recognition event and a subsequent stabilization, is supported by the use of a new cell binding assay that provides a quantitative measure of intercellular binding strengths. Radioactive single cells are brought into ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1982.240180408

    authors: McClay DR,Marchase RB

    更新日期:1982-01-01 00:00:00

  • Cell Cycle-Dependent Localization of Dynactin Subunit p150 glued at Centrosome.

    abstract::p150(glued) is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150(glued) also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25160

    authors: Chen TY,Syu JS,Han TY,Cheng HL,Lu FI,Wang CY

    更新日期:2015-09-01 00:00:00

  • B cell epitopes in the intrinsically disordered regions of neuraminidase and hemagglutinin proteins of H5N1 and H9N2 avian influenza viruses for peptide-based vaccine development.

    abstract::Avian influenza viruses (AIV) are very active in several parts of the globe and are the cause of huge economic loss for the poultry industry and also human fatalities. Three dimensional modeling was carried out for neuraminidase (NA) and hemagglutinin (HA) proteins of AIV. The C-score, estimated TM-Score, and estimate...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29017

    authors: Ramamurthy M,Sankar S,Abraham AM,Nandagopal B,Sridharan G

    更新日期:2019-10-01 00:00:00

  • Virtual screening of naphthoquinone analogs for potent inhibitors against the cancer-signaling PI3K/AKT/mTOR pathway.

    abstract::The PI3K/AKT/mTOR pathway is one of the most commonly disrupted signaling pathways that plays a role in the development and pathogenicity of multiple cancers. Therefore, the critical proteins of this pathway have been targeted for anticancer therapy. The scientific community has increasingly been realizing the anti-ca...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27100

    authors: Rehan M,Bajouh OS

    更新日期:2018-10-08 00:00:00

  • Recent Advances in Mechanobiology of Osteosarcoma.

    abstract::Mechanotransduction is a key process by which cells perceive extracellular mechanical cues/intercellular physical interactions and transform them into intracellular biochemical signals. This physiological process is crucial during bone development and bone remodeling throughout childhood and adult life, whereas severa...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.25660

    authors: Adamopoulos C,Gargalionis AN,Piperi C,Papavassiliou AG

    更新日期:2017-02-01 00:00:00

  • Differentiation in Acanthamoeba castellanii is induced by specific monoclonal antibodies.

    abstract::Monoclonal antibodies that bind a large molecular weight plasma membrane protein of Acanthamoeba castellanii cause the cells to differentiate. A different monoclonal antibody that binds specifically to the major plasma membrane protein has no effect upon cell division or differentiation. The induction of differentiati...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240290410

    authors: Villemez CL,Carlo PL,Russell MA

    更新日期:1985-01-01 00:00:00

  • Are primary cultures realistic models of prostate cancer?

    abstract::Primary cultures fill a unique niche among the repertoire of in vitro model systems available to investigate the biology of the normal and malignant human prostate. This review summarizes some of the properties of primary cultures, with special emphasis on two questions: are primary cultures from adenocarcinomas reall...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.10691

    authors: Peehl DM

    更新日期:2004-01-01 00:00:00

  • Nuclear import and DNA binding of human papillomavirus type 45 L1 capsid protein.

    abstract::During the life cycle of human papillomaviruses (HPVs), the L1 capsid proteins seem to enter the nucleus twice: once after the virions infect the cells, and later during the productive phase when they assemble the replicated HPV genomic DNA into infectious virions. We established for the high-risk HPV45 that when digi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Nelson LM,Rose RC,LeRoux L,Lane C,Bruya K,Moroianu J

    更新日期:2000-08-02 00:00:00

  • Reciprocal modulation of histone deacetylase inhibitors sodium butyrate and trichostatin A on the energy metabolism of breast cancer cells.

    abstract::Tumor cells display different bioenergetic profiles when compared to normal cells. In the present work we showed metabolic reprogramming by means of inhibitors of histone deacetylase (HDACis), sodium butyrate and trichostatin A in breast cancer cells representing different stages of aggressiveness and metabolic profil...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25036

    authors: Rodrigues MF,Carvalho É,Pezzuto P,Rumjanek FD,Amoêdo ND

    更新日期:2015-05-01 00:00:00

  • Golgi/granule processing of peptide hormone and neuropeptide precursors: a minireview.

    abstract::Proteolytic processing of precursor proteins is a phylogenetically ancient and widely used mechanism for producing biologically active peptides. Proteolytic cleavage of proproteins begins only after transport to the Golgi apparatus has been completed and in most systems may continue for many hours within newly formed ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.240240204

    authors: Steiner DF,Docherty K,Carroll R

    更新日期:1984-01-01 00:00:00

  • Lipophilic statins antagonistically alter the major epithelial-to-mesenchymal transition signaling pathways in breast cancer stem-like cells via inhibition of the mevalonate pathway.

    abstract::Resistance to therapies, recurrence, and metastasis remain challenging issues for breast cancer patients, particularly for triple-negative and breast cancer stem cells. The activation of the epithelial-to-mesenchymal transition (EMT) plays an indispensable role in the poor prognosis of those types. The accumulating pr...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27544

    authors: Koohestanimobarhan S,Salami S,Imeni V,Mohammadi Z,Bayat O

    更新日期:2018-09-06 00:00:00

  • Inhibition of GSK-3beta decreases NF-kappaB-dependent gene expression and impairs the rat liver regeneration.

    abstract::Serine-threonine protein kinase glycogen synthase kinase (GSK)-3 is involved in regulation of many cell functions, but its role in regulating liver regeneration is unknown. Here we investigated the effects of GSK-3beta inhibition on liver regeneration after partial hepatectomy in the rat. The potent and selective GSK-...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21358

    authors: Chen H,Yang S,Yang Z,Ma L,Jiang D,Mao J,Jiao B,Cai Z

    更新日期:2007-12-01 00:00:00

  • Organochlorine and organophosphorus pesticides and bladder cancer: A case-control study.

    abstract:BACKGROUND:Exposure to pesticides is associated with an increase in the incidence of cancer. We aimed to investigate the association of serum organochlorine pesticides (OCPs) and organophosphorus pesticides (OPs) levels and GSTM1/GSTT1 gene polymorphism with bladder cancer (BC). METHODS:This study was performed on 57 ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28746

    authors: Mortazavi N,Asadikaram G,Ebadzadeh MR,Kamalati A,Pakmanesh H,Dadgar R,Moazed V,Paydar P,Fallah H,Abolhassani M

    更新日期:2019-09-01 00:00:00