Abstract:
BACKGROUND AND OBJECTIVES:Routine serologic D typing does not distinguish between weak D subtypes and partial D phenotypes. The goal of this study was to validate the performance of the ID RHD XT genotyping assay. MATERIAL AND METHODS:Previously serotyped samples for D antigen (n = 1000; 16% weak D serotyped donors) were analysed. The reference methods used for comparison were licensed serology tests for D antigen phenotype, and bidirectional sequencing (BDS) for weak D type confirmation and HPA-1 phenotype prediction. Discrepancies were solved with BDS and BLOODchip® Reference. RESULTS:There were no system failure, a 100% call rate and no inconclusive results. ID RHD XT correctly called all (88/88) weak D types 1, 2 and 3. Review of other 87 apparent discrepancies identified a small number of serology errors and showed that ID RHD XT correctly signalled the presence of other RHD variants which were further confirmed by BDS and BLOODchip® Reference. The predicted HPA-1 phenotype by ID RHD XT was 100% concordant with BDS. CONCLUSION:ID RHD XT genotype predictions for high-prevalence RhD negative and weak D types 1, 2 and 3 as well as for HPA-1a/HPA-1b antigens were accurate, which is of clinical significance in guiding transfusion needs.
journal_name
Vox Sangjournal_title
Vox sanguinisauthors
Molano A,Apraiz I,España P,Azkarate M,Vesga MÁ,Rubia M,Piedrabuena M,Puente F,Veldhuisen B,van der Schoot E,Tejedor D,López Mdoi
10.1111/vox.12701subject
Has Abstractpub_date
2018-10-01 00:00:00pages
694-700issue
7eissn
0042-9007issn
1423-0410journal_volume
113pub_type
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