The dietary flavone luteolin epigenetically activates the Nrf2 pathway and blocks cell transformation in human colorectal cancer HCT116 cells.

Abstract:

:Colorectal cancer remains a leading malignancy in humans. The importance of epigenetic modification in the development of this disease is now being recognized. The reversible and dynamic nature of epigenetic modifications provides a promising strategy in colorectal cancer chemoprevention and treatment. Luteolin (LUT), a flavone dietary phytochemical, can modulate various signaling pathways involved in carcinogenesis. Many studies have demonstrated that LUT inhibits colorectal carcinogenesis by activating the Nuclear factor erythroid 2-related factor 2 (Nrf2)/antioxidant-responsive element (ARE) pathway. However, the potential epigenetic mechanism underlying Nrf2/ARE pathway activation remains unclear. In this study, we aimed to explore the anticancer potential of LUT in human colon cancer cells and the epigenetic regulation of the Nrf2/ARE pathway. Specifically, our data showed that LUT suppressed cell proliferation and cellular transformation of HCT116 and HT29 cells in a dose-dependent manner. Additionally, quantitative real-time polymerase chain reaction and Western blot analysis were performed to determine the mRNA and protein expression of Nrf2 and its downstream genes after LUT treatment. Bisulfite genomic sequencing revealed that methylation of the Nrf2 promoter region was decreased by LUT, corresponding with the increased mRNA expression of Nrf2. Decreased protein levels and enzyme activities of epigenetic modifying enzymes, such as DNA methyltransferases (DNMTs) and histone deacetylases (HDACs), were also observed in LUT-treated HCT116 cells. In summary, our findings suggest that LUT may exert its antitumor activity in part via epigenetic modifications of the Nrf2 gene with subsequent induction of its downstream antioxidative stress pathway.

journal_name

J Cell Biochem

authors

Zuo Q,Wu R,Xiao X,Yang C,Yang Y,Wang C,Lin L,Kong AN

doi

10.1002/jcb.27275

subject

Has Abstract

pub_date

2018-11-01 00:00:00

pages

9573-9582

issue

11

eissn

0730-2312

issn

1097-4644

journal_volume

119

pub_type

杂志文章
  • Matrix influence on the tumor cell stimulation of fibroblast collagenase production.

    abstract::Cocultures of rabbit fibroblasts and mouse B-16 melanoma cells produce increased levels of collagenase against type I collagen. This stimulatory effect was also found when fibroblasts were cultured in conditioned media from tumor cells. However, the level of the stimulatory factor in conditioned media was influenced b...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240280107

    authors: Biswas C

    更新日期:1985-01-01 00:00:00

  • Role of pertussis toxin-sensitive G-proteins in intracellular Ca2+ release and apoptosis induced by inhibiting cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels in HepG2 human hepatoblastoma cells.

    abstract::Previously, we have reported that inhibition of cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels by glibenclamide induced intracellular Ca2+ release from IP(3)-sensitive stores and apoptosis in HepG2 human hepatoblastoma cells (Kim JA, Kang YS, Lee SH, Lee EH, Yoo BH, Lee YS. 1999. Biochem Bioph...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/1097-4644(20010401)81:1<93::aid-jcb1026>3.

    authors: Kim JA,Kang YS,Lee SH,Lee EH,Lee YS

    更新日期:2001-01-01 00:00:00

  • miR-21 modulates the ERK-MAPK signaling pathway by regulating SPRY2 expression during human mesenchymal stem cell differentiation.

    abstract::The ERK-MAPK signaling pathway plays a pivotal role during mesenchymal stem cell (MSC) differentiation. Studies have demonstrated that ERK-MAPK promotes adipogenesis and osteogenesis through the phosphorylation of differentiation-associated transcription factors and that it is the only active signaling in all three li...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24479

    authors: Mei Y,Bian C,Li J,Du Z,Zhou H,Yang Z,Zhao RC

    更新日期:2013-06-01 00:00:00

  • LncRNA MALAT1 promotes oxidized low-density lipoprotein-induced autophagy in HUVECs by inhibiting the PI3K/AKT pathway.

    abstract::Emerging evidence suggests that long noncoding RNAs (lncRNAs) are involved in many biological processes, such as cell growth, differentiation, apoptosis, and autophagy. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), highly expressed in endothelial cells, is well conserved and implicated in endothelia...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27694

    authors: Li S,Pan X,Yang S,Ma A,Yin S,Dong Y,Pei H,Bi X,Li W

    更新日期:2019-03-01 00:00:00

  • MicroRNA-223 is a key factor in osteoclast differentiation.

    abstract::MicroRNAs (miRNAs) are a class of noncording RNAs that control gene expression by translational inhibition and messenger RNAs (mRNAs) degradation in plants and animals. Although miRNAs have been implicated in developmental and homeostatic events of vertebrates and invertebrates, the role of miRNAs in bone metabolism h...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21335

    authors: Sugatani T,Hruska KA

    更新日期:2007-07-01 00:00:00

  • Immunomodulatory role of 1,25-dihydroxyvitamin D3.

    abstract::The active vitamin D metabolite 1,25-dihydroxyvitamin D3 [1,25-D3] is thought to promote many of its actions through interaction with a specific intracellular receptor. The discovery of such receptors in monocytes and activated lymphocytes has led investigators to evaluate the role of the hormone on the immune system....

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.240490106

    authors: Lemire JM

    更新日期:1992-05-01 00:00:00

  • Novel identification of peripheral dopaminergic D2 receptor in male germ cells.

    abstract::Dopamine is a recognized modulator in the central nervous system (CNS) and peripheral organ functions. The presence of peripheral dopamine receptors outside the CNS has suggested an intriguing interaction between the nervous system and other functional systems, such as the reproductive system. In the present study we ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21037

    authors: Otth C,Torres M,Ramírez A,Fernandez JC,Castro M,Rauch MC,Brito M,Yañez AJ,Rodríguez-Gil JE,Slebe JC,Concha II

    更新日期:2007-01-01 00:00:00

  • Intrinsic or acquired drug resistance and metastasis: are they linked phenotypes?

    abstract::Evidence is reviewed which suggests a linkage may exist between certain forms of de novo or acquired drug resistance and metastasis. This includes the finding that expression of certain dominantly acting mutant oncogenes or tumor suppressor genes, i.e., genes which normally act to "drive" tumor progression and metasta...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.240560108

    authors: Kerbel RS,Kobayashi H,Graham CH

    更新日期:1994-09-01 00:00:00

  • The protective effect exerted by ascorbic acid on DNA fragmentation of human leukocytes induced by Lachesis muta muta venom.

    abstract::The objective of this study was to evaluate the genotoxic and mutagenic effects of the toxins present in Lachesis muta muta's venom on human peripheral blood leukocytes and the protective potential of ascorbic acid on DNA fragmentation. The venom of L. muta muta was incubated in different concentrations (1, 2.5, 5, 7....

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27628

    authors: Cardoso Trento MV,de Faria Eleutério MW,Silva Abreu T,Andrade Machado GH,Cesar PHS,Assaid Simão A,Marcussi S

    更新日期:2019-03-01 00:00:00

  • The activity of aminoacyl-tRNA synthetase-interacting multi-functional protein 1 (AIMP1) on endothelial cells is mediated by the assembly of a cytoskeletal protein complex.

    abstract::AIMP1 was first found as a factor associated with the aminoacyl-tRNA synthetase (ARS) complex. However, it is also secreted and acts on different target cells such as endothelial cells, macrophages, and fibroblasts as an extracellular regulator, respectively, of angiogenesis, inflammatory responses and dermal regenera...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23104

    authors: Jackson VC,Dewilde S,Albo AG,Lis K,Corpillo D,Canepa B

    更新日期:2011-07-01 00:00:00

  • Myosin translocation in retinal pericytes during free-radical induced apoptosis.

    abstract::Vascular pathologies induced by ischemia/reperfusion involve the production of reactive oxygen species (ROS) that in part cause tissue injury. The production of ROS that occurs upon reperfusion activates specific second messenger pathways. In diabetic retinopathy there is a characteristic loss of the microvascular per...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Shojaee N,Patton WF,Hechtman HB,Shepro D

    更新日期:1999-10-01 00:00:00

  • Bevacizumab and ranibizumab on microvascular endothelial cells: A comparative study.

    abstract::Given its broad effects in endothelium, vascular endothelial growth factor (VEGF) represents the primary rate-limiting step of angiogenesis. Therefore, VEGF targeting therapies were soon developed. Bevacizumab and ranibizumab are two of these therapeutic agents already in clinical use. Bevacizumab was first used for c...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22378

    authors: Costa R,Carneiro A,Rocha A,Pirraco A,Falcão M,Vasques L,Soares R

    更新日期:2009-12-15 00:00:00

  • Inhibition of cell growth and induction of apoptosis in non-small cell lung cancer cells by delta-tocotrienol is associated with notch-1 down-regulation.

    abstract::Lung cancer is the leading cause of death among all cancers. Non-small cell lung cancer accounts for 80% of lung cancer with a 5-year survival rate of 16%. Notch pathway, especially Notch-1 is up-regulated in a subgroup of non-small cell lung cancer patients. Since Notch-1 signaling plays an important role in cell pro...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23184

    authors: Ji X,Wang Z,Geamanu A,Sarkar FH,Gupta SV

    更新日期:2011-10-01 00:00:00

  • Differential effects of chemotherapeutic drugs versus the MDM-2 antagonist nutlin-3 on cell cycle progression and induction of apoptosis in SKW6.4 lymphoblastoid B-cells.

    abstract::We have compared the cytotoxic/cytostatic responses of the SKW6.4 lymphoblastoid B-cells to the alkylating agent chlorambucil, the purine analog fludarabine, the non-genotoxic activator of the p53 pathway, Nutlin-3, used alone or in association with the death-inducing ligand recombinant TRAIL. Exposure to chlorambucil...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21649

    authors: Barbarotto E,Corallini F,Rimondi E,Fadda R,Mischiati C,Grill V,Vaccarezza M,Celeghini C

    更新日期:2008-05-15 00:00:00

  • Biochemical evidence on the potential role of methyl mercury in hepatic glucose metabolism through inflammatory signaling and free radical pathways.

    abstract::Methylmercury (MeHg) is an extremely important environmental toxicant posing serious health risks to human health and a big source of environmental pollutant. Numerous evidence available showing a link between nervous system toxicity and MeHg exposure. Other forms of mercury are reason of metabolic toxic effects and a...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28899

    authors: Maqbool F,Bahadar H,Hassani S,Niaz K,Baeeri M,Rahimifard M,Ghasemi-Niri SF,Abdollahi M

    更新日期:2019-09-01 00:00:00

  • Transcriptional regulation of Runx2 by HSP90 controls osteosarcoma apoptosis via the AKT/GSK-3β/β-catenin signaling.

    abstract::Osteosarcoma (OS) is the most malignant primary bone tumor in children and adolescents with limited treatment options and poor prognosis. Recently, aberrant expression of Runx2 has been found in OS, thereby contributing to the development, and progression of OS. However, the upstream signaling molecules that regulate ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26260

    authors: Liang GH,Liu N,He MT,Yang J,Liang ZJ,Gao XJ,Rahhal AH,He QY,Zhang HT,Zha ZG

    更新日期:2018-01-01 00:00:00

  • SCF FBXW17 E3 ubiquitin ligase regulates FBXL19 stability and cell migration.

    abstract::The Skp1-Cul1-F-box protein (SCF) E3 ligase complex is one of the largest ubiquitin E3 ligase families. FBXL19, a F-box protein in SCFFBXL19 E3 ligase complex, regulates a variety of cellular responses including cell migration. We have shown that FBXL19 is not stable and its degradation is mediated by the ubiquitin-pr...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29860

    authors: Dong S,Wei J,Bowser RK,Chen BB,Mallampalli RK,Miao J,Ye Q,Tran KC,Zhao Y,Zhao J

    更新日期:2020-10-14 00:00:00

  • Modulation of GST P1-1 activity by polymerization during apoptosis.

    abstract::Glutathione S-transferases (GSTs, EC 2.5.1.18) belong to a large family of functionally different enzymes that catalyze the S-conjugation of glutathione with a wide variety of electrophilic compounds including carcinogens and anticancer drugs. Drug resistance may result from reduction in apoptosis of neoplastic cells ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Bernardini S,Bernassola F,Cortese C,Ballerini S,Melino G,Motti C,Bellincampi L,Iori R,Federici G

    更新日期:2000-04-01 00:00:00

  • Low expression of NCOA5 predicts poor prognosis in human cervical cancer and promotes proliferation, migration, and invasion of cervical cancer cell lines by regulating notch3 signaling pathway.

    abstract::Nuclear receptor coactivator 5 (NCOA5) specifically enhances estrogen receptor α-modulated transcriptional activity. As a novel tumor suppressor, depletion of NCOA5 is associated with the development of a variety of tumors, but its function in cervical cancer is currently unclear. In this study, we addressed how expre...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27911

    authors: Liang Y,Zhang T,Shi M,Zhang S,Guo Y,Gao J,Yang X

    更新日期:2019-04-01 00:00:00

  • Characterization of fertilization-modulated myelin basic protein kinases from sea star: regulation of Mapk.

    abstract::The myelin basic protein (MBP)-phosphorylating enzymes present during maturation and early embryogenesis of the sea star (Pisaster ochraceus) were investigated. The major maturation-activated MBP kinase (p45 Mapk) was molecularly cloned based on tryptic sequence information obtained with the purified enzyme and shown ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Lefebvre DL,Charest DL,Yee A,Crawford BJ,Pelech SL

    更新日期:1999-11-01 00:00:00

  • Neuroprotective effects of PrxI over-expression in an in vitro human Alzheimer's disease model.

    abstract::Peroxiredoxins are ubiquitous proteins that recently attracted major interests in view of the strict correlation observed in several cell lines and/or tissues between different levels of their expression and the increased capacity of cells to survive in different pathophysiological conditions. They are recently consid...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24412

    authors: Cimini A,Gentile R,Angelucci F,Benedetti E,Pitari G,Giordano A,Ippoliti R

    更新日期:2013-03-01 00:00:00

  • The design, synthesis, and characterization of tight-binding inhibitors of calmodulin.

    abstract::Based on a consideration of the probable structure of calmodulin and some natural peptides known to interact with it, two calmodulin-binding peptides were designed. These peptides bind to calmodulin in helical conformations and are capable of forming electrostatic and hydrophobic interactions with calmodulin. Their di...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240290204

    authors: DeGrado WF,Prendergast FG,Wolfe HR Jr,Cox JA

    更新日期:1985-01-01 00:00:00

  • microRNA-542-5p protects against acute lung injury in mice with severe acute pancreatitis by suppressing the mitogen-activated protein kinase signaling pathway through the negative regulation of P21-activated kinase 1.

    abstract::Severe acute pancreatitis (SAP) is a condition associated with high rates of mortality and lengthy hospital stays. In the current study, SAP mouse models were established in BALB/c wild-type and P21-activated kinase 1 (PAK1) knockdown mice with the objective of determining the expression of microRNA-542-5p (miR-542-5p...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27356

    authors: Wu XM,Ji KQ,Wang HY,Zhao Y,Jia J,Gao XP,Zang B

    更新日期:2019-01-01 00:00:00

  • The effects of peptides with estrogen-like activity on cell proliferation and energy metabolism in human derived vascular smooth muscle cells.

    abstract::Hormone replacement therapy (HRT) for post-menopausal symptoms in diabetes is associated with increased risk of coronary heart disease and stroke. Therefore, there is a need for new HRT with no adverse effects on diabetic post-menopausal women. We developed peptides as potential estrogen mimetic compounds and now we e...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22627

    authors: Somjen D,Knoll E,Gayer B,Kasher R,Kohen F,Stern N

    更新日期:2010-08-01 00:00:00

  • Epigenetic DNA methylation in radiation biology: on the field or on the sidelines?

    abstract::DNA methylation has been studied with regard to chemotherapeutics for a number of years. The radiation field has just begun to look at this in the context of radiotherapy or radiation exposure. So far, the data suggest that radiation induces epigenetic reprogramming which indicates a purposeful response that influence...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.24959

    authors: Zielske SP

    更新日期:2015-02-01 00:00:00

  • Protective effect of HSP27 in atherosclerosis and coronary heart disease by inhibiting reactive oxygen species.

    abstract:OBJECTIVE:To clarify the mechanism of heat shock protein 27 (HSP27) as a diagnostic biomarker in coronary heart disease (CHD) and atherosclerosis (AS). METHOD:Expressions of HSP27 in patients with CHD and healthy controls were determined by enzyme-linked immunosorbent assay and the expressions of HSP27 in aortas of pa...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26575

    authors: Zhang HL,Jia KY,Sun D,Yang M

    更新日期:2019-03-01 00:00:00

  • Molecular interactions between p-coumaric acid and snake venom toxins.

    abstract::A large number of natural compounds, such as phenolic compounds, have been scientifically evaluated in the search for enzyme inhibitors. The interactions between the phenolic compound p-coumaric acid and the enzymes present in snake venoms (used as research tools) were evaluated in vitro and in silico. The p-coumaric ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28721

    authors: Cesar PHS,Cardoso Trento MV,Sales TA,Marques TR,Braga MA,Ramalho TC,Marcussi S

    更新日期:2019-09-01 00:00:00

  • Inhibitory helix-loop-helix transcription factors Id1/Id3 promote bone formation in vivo.

    abstract::Bone formation is under the control of a set of transcription factors. Ids are inhibitory helix-loop-helix (HLH) transcription factors and expression of Id genes in osteoblasts is under the control of calciotropic agents such as BMP and vitamin D. However, the function of Ids during bone formation in vivo has not yet ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20154

    authors: Maeda Y,Tsuji K,Nifuji A,Noda M

    更新日期:2004-10-01 00:00:00

  • Mechanisms regulating c-met overexpression in liver-metastatic B16-LS9 melanoma cells.

    abstract::Liver selected B16-LS9 melanoma cells show a dramatic overexpression of the proto-oncogene c-met, the cellular receptor for hepatocyte growth factor/scatter factor. As a consequence, c-met becomes constitutively active, and the cells become more responsive to hepatocyte growth factor stimulation. We have investigated ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/1097-4644(20010601)81:3<477::aid-jcb1061>3

    authors: Elia G,Ren Y,Lorenzoni P,Zarnegar R,Burger MM,Rusciano D

    更新日期:2001-01-01 00:00:00

  • Effects of serum on calcium mobilization in the submandibular cell line A253.

    abstract::The effects of serum on inositol 1,4,5-trisphosphate (IP3) formation and Ca2+ mobilization in the human submandibular cell line A253 were studied. Exposure of A253 cells to fetal bovine serum (FBS) elicited a 3.3-fold increase in IP3 formation and a concentration-dependent transient increase in cytosolic free Ca2+ con...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Sun X,Mörk AC,Helmke RJ,Martinez JR,Zhang GH

    更新日期:1999-06-15 00:00:00