Abstract:
BACKGROUND:We have previously identified the upregulation of the innate immune response, neutrophil activation, and apoptosis during anaphylaxis using a microarray approach. This study aimed to validate the differential gene expression and investigate protein concentrations of "hub genes" and upstream regulators during anaphylaxis. METHODS:Samples were collected from patients with anaphylaxis on their arrival at the emergency department, and after 1 and 3 h. mRNA levels of 11 genes (interleukin-6 [IL-6], IL-10, oncostatin M [OSM], S100A8, S100A9, matrix metalloproteinase 9 [MMP9], FASL, toll-like receptor 4 [TLR4], MYD88, triggering receptor expressed on myeloid cells 1 [TREM1], and cluster of differentiation 64 [CD64]) were measured in peripheral blood leucocytes using qPCR. Serum protein concentrations were measured by ELISA or cytometric bead array for 6 of these candidates. RESULTS:Of 69 anaphylaxis patients enrolled, 36 (52%) had severe reactions, and 38 (55%) were female. Increases in both mRNA and protein of IL-10, S100A9, MMP9, and TREM1 were observed. OSM, S100A8, TLR4, and CD64 were upregulated and IL-6 protein concentrations were increased during anaphylaxis. Both FASL and soluble Fas ligand decreased during anaphylaxis. CONCLUSION:These results provide evidence for the involvement of innate immune pathways and myeloid cells during human anaphylaxis, validating previous microarray findings. Elevated S100A8, S100A9, TLR4, and TREM1 expression, and increased S100A9 and soluble TREM1 protein concentrations strongly suggest that neutrophils are activated during acute anaphylaxis.
journal_name
J Innate Immunjournal_title
Journal of innate immunityauthors
Francis A,Bosio E,Stone SF,Fatovich DM,Arendts G,MacDonald SPJ,Burrows S,Brown SGAdoi
10.1159/000492301subject
Has Abstractpub_date
2019-01-01 00:00:00pages
63-73issue
1eissn
1662-811Xissn
1662-8128pii
000492301journal_volume
11pub_type
杂志文章abstract::Fibroblasts are known to eliminate intracellular bacteria, but the lethal hit of the bactericidal mechanism has not been defined. We show that primary embryonic and established fibroblasts can be induced by interferons or by intracellular bacterial infection to express a perforin-like mRNA previously described as macr...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000345249
更新日期:2013-01-01 00:00:00
abstract::Listeria monocytogenes is a facultative intracellular pathogen which can infect Drosophila melanogaster. Upon infection, Drosophila mounts an immune response including antimicrobial peptide production and autophagy activation. A set of previously published results prompted us to study the role of the deubiquitinating ...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000360293
更新日期:2014-01-01 00:00:00
abstract::NK cells play a central role in innate immunity, acting directly through cell-mediated cytotoxicity and by secreting cytokines. TNFα activation of TNFR2 enhances NK cell cytotoxicity, but its effects on the other essential function of NK cells - cytokine production, for which IFNγ is paramount - are poorly defined. We...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000448077
更新日期:2016-01-01 00:00:00
abstract::Particulate systems in the form of liposomes, polymeric micelles, polymeric nano- and microparticles, and many others offer a rational approach for selective delivery of therapeutic agents to the macrophage from different physiological portals of entry. Particulate targeting of macrophages and intracellular drug relea...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000339153
更新日期:2012-01-01 00:00:00
abstract::Porphyromonas gingivalis produces proteases capable of degrading cytokines, host heme proteins and some antimicrobial peptides. In this study, we show that P. gingivalis culture supernatants fully or partially degrade human neutrophil peptide alpha-defensins and human beta-defensins after 30 min. This observation sugg...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000181015
更新日期:2009-01-01 00:00:00
abstract::Early interactions between natural killer (NK) and dendritic cells (DC) shape the immune response at the frontier of innate and adaptive immunity. Activated NK cells participate in maturation or deletion of DCs that remain immature. We previously demonstrated that infection with a high virulence (HV) population of the...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000350242
更新日期:2013-01-01 00:00:00
abstract::Killer immunoglobulin-like receptors (KIRs) recognize class I major histocompatibility complex molecules and participate in the calibration of activation thresholds during human natural killer (NK) cell development. The stochastic expression pattern of the KIR repertoire follows the product rule, meaning that the prob...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000323929
更新日期:2011-01-01 00:00:00
abstract::The impact of spleen tyrosine kinase (Syk) signaling might be prominent in lupus because (i) Syk is a shared downstream signaling molecule among circulating immune complex, LPS, and (1→3)-β-D-glucan (BG), and (ii) all of these factors are detectable in the serum of Fc gamma receptor IIb-deficient (FcgRIIb-/-) mice wit...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000509111
更新日期:2020-01-01 00:00:00
abstract::There is increasing evidence that the immune system is regulated by circadian rhythms. A wide range of immune parameters, such as the number of red blood cells and peripheral blood mononuclear cells as well as the level of critical immune mediators, such as cytokines, undergo daily fluctuations. Current experimental d...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000342427
更新日期:2013-01-01 00:00:00
abstract::Unwarranted overproduction of cytokines, such as interleukin (IL)-1β, can cause moderate to severe pathological complications, and thus elaborate mechanisms are needed to regulate its onset and termination. One such, well-known, mechanism is endotoxin tolerance, generally described as controlling lipopolysaccharide To...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000371517
更新日期:2015-01-01 00:00:00
abstract::Ficolins are a group of oligomeric lectins with subunits consisting of both collagen-like and fibrinogen-like domains. The majority of ficolins identified in vertebrates and invertebrates to date recognize N-acetylglucosamine (GlcNAc). X-ray crystallographic analysis of human ficolins has shown that the fibrinogen-lik...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000228160
更新日期:2010-01-01 00:00:00
abstract::Streptococcus pyogenes is an important human pathogen that causes a variety of diseases including life-threatening invasive diseases, such as toxic shock and deep tissue infections. Although S. pyogenes are classically considered extracellular pathogens, a clinical significance of an intracellular source has been emph...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000317635
更新日期:2010-01-01 00:00:00
abstract::The host innate immune response, including the production of type-I IFN, represents the primary line of defense against invading viral pathogens. Of the hundreds of IFN-stimulated genes (ISGs) discovered to date, ISG15 was one of the first identified and shown to encode a ubiquitin-like protein that functions, in part...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000226245
更新日期:2009-01-01 00:00:00
abstract::Atherosclerosis is a chronic inflammatory disease characterized by dyslipidemia and accumulation of lipids in the arterial intima, with activation of both innate and adaptive immunity. Reciprocally, dyslipidemia associated with atherosclerosis can perturb normal immune function. Natural killer T (NKT) cells are a spec...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000296915
更新日期:2010-01-01 00:00:00
abstract::L-ficolin, one of the complement lectins found in human serum, is a novel pattern recognition molecule that can specifically bind to microbial carbohydrates, thereby activating the lectin complement pathway and mounting a protective innate immune response. However, little is known about the role of L-ficolin during vi...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000335670
更新日期:2012-01-01 00:00:00
abstract::Psoriasis is one of the most common immune-mediated disorders. There is evidence that it is mediated by Th1 and, more recently, Th17 cells. The cytokine pattern, particularly the dominance of TNF-α, implicates the innate immune system in psoriasis pathogenesis. Of the many components of the innate immune system known ...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000328011
更新日期:2011-01-01 00:00:00
abstract::Hemolytic uremic syndrome (HUS), a vascular disease characterized by hemolytic anemia, thrombocytopenia, and acute renal failure, is caused by enterohemorrhagic Shiga toxin (Stx)-producing bacteria, which mainly affect children. Besides Stx, the inflammatory response mediated by neutrophils (PMN) is essential to HUS e...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000445770
更新日期:2016-01-01 00:00:00
abstract::Immunoglobulin A nephropathy (IgAN) is the most common form of glomerulonephritis throughout the world. A majority (approx. 60%) of patients with IgAN experience disease exacerbations associated with an acute respiratory or gastrointestinal illness that appears to represent a viral infection. However, the exact mechan...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000345255
更新日期:2013-01-01 00:00:00
abstract::The complement-like protein thioester-containing protein 1 (TEP1) is the hallmark effector molecule against Plasmodium ookinetes in the malaria vector Anopheles gambiae. We have previously shown that the knockdown of the noncatalytic clip domain serine protease CLIPA2 increased TEP1-mediated killing rendering mosquito...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000443883
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Oncolytic vesicular stomatitis virus (VSV) can be delivered intravenously to target primary and metastatic lesions, but the interaction between human peripheral blood leukocytes (PBLs) and VSV remains poorly understood. Our study aimed to assess the overall immunological consequences of ex vivo infection of ...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000485143
更新日期:2018-01-01 00:00:00
abstract::We characterized the dual oxidase (Duox) gene in the major Indian malaria vector Anopheles stephensi, which regulates the generation of reactive oxygen species. The AsDuox gene encodes for a 1,475-amino-acid transmembrane protein that contains an N-terminal noncytoplasmic heme peroxidase domain, a calcium-binding doma...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000497417
更新日期:2019-01-01 00:00:00
abstract::Histones are small basic proteins and highly conserved among eukaryotes. Their main function is binding, packaging and organizing of DNA in the nucleus, but extracellular histones are also potent antimicrobial proteins. Here we found that Streptococcus pyogenes - an important human pathogen - protects itself from hist...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000448039
更新日期:2016-01-01 00:00:00
abstract::Pattern recognition is an integral part of the innate immune system. The human contact system has been shown to interact with the surface of many bacterial and fungal pathogens, and once activated leads to the generation of antimicrobial peptides and the proinflammatory mediator bradykinin. Here we show that apart fro...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000203700
更新日期:2009-01-01 00:00:00
abstract::Melanoma differentiation-associated gene 5 (MDA5) is a pattern recognition receptor that recognizes cytoplasmic viral double-stranded RNA (dsRNA) and initiates rapid innate antiviral responses. MDA5 forms a filament-like multimer along the dsRNA leading to oligomerization, which in turn activates the adaptor protein m...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000480740
更新日期:2018-01-01 00:00:00
abstract::The phagocyte NADPH oxidase, crucial for innate immunity, is dormant in resting cells, but becomes activated during phagocytosis to produce superoxide, a precursor of microbicidal oxidants. In activation of the oxidase, the multidomain protein p67(phox)plays a central role: it translocates to the membrane as a ternary...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000235656
更新日期:2009-01-01 00:00:00
abstract::As a key molecule in the antiviral innate immune response, the activation of TANK-binding kinase 1 (TBK1) is under tight regulation. In this report, we identified phosphatidylserine-specific phospholipase PLA1A as a host factor that modulates the TBK1 activation. Knockdown of PLA1A expression suppressed the innate imm...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000489832
更新日期:2018-01-01 00:00:00
abstract::Eiger is the sole TNF family member found in Drosophila melanogaster. This signaling molecule is induced during infection and is required for an appropriate immune response to many microbes; however, little is known about where eiger is produced. Here, we show that eiger is made in the fly's fat body during a Salmonel...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000315050
更新日期:2010-01-01 00:00:00
abstract::Elevated inflammation and altered immune responses are features found in atopic asthmatic airways. Recent studies indicate γ-tocopherol (GT) supplementation can suppress airway inflammation in allergic asthma. We studied the effects of in vitro GT supplementation on receptor-mediated phagocytosis and expression of cel...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000350234
更新日期:2013-01-01 00:00:00
abstract::To protect against viral infection, the immune response is critically dependent on innate sensing mechanisms to provide rapid detection of pathogens and allow for the development of an appropriate adaptive immune response. Mounting evidence suggests that mechanistic differences in the sensing of viruses by the innate ...
journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000226247
更新日期:2009-01-01 00:00:00
abstract:BACKGROUND:Pseudomonas aeruginosa (PS) infection results in severe morbidity and mortality, especially in immune-deficient populations. Aerobic exercise (AE) modulates the immune system, but its effects on the outcomes of pulmonary PS infection in elderly mice are unknown. METHODS:BALB/c mice (24 weeks old) were rando...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000488953
更新日期:2018-01-01 00:00:00