Nebulization as a tool for photosensitizer delivery to the respiratory tract.

Abstract:

:To this day, any photosensitizers for the photodynamic treatment of pulmonary illnesses have been administered intravenously. There is, however, an intrinsic difficulty in reaching the target cells or bacteria in the respiratory system. Nebulization could overcome distribution problems and alleviate side effects by delivering the photosensitizers directly to the lungs. In this study, we evaluated the viability of three photosensitizers (indocyanine green, the chlorine Photodithazine, and the porphyrin Photogem) was evaluated comparatively in a jet nebulizer. Quantitative analysis was performed by looking at the droplet size, extent of nebulization, output over time and stability of the solutions. All of the tested photosensitizers were found to be adequately nebulized. We also demonstrated the delivery of indocyanine green to the pulmonary tract and its activation with infrared light in a murine model using extracorporeal detection of fluorescence. This was an important step toward clinical implementation of the extracorporeally illuminated photodynamic inactivation of pneumonia, recently demonstrated in vivo by this research group.

journal_name

J Biophotonics

journal_title

Journal of biophotonics

authors

Kassab G,Geralde MC,Inada NM,Achiles AE,Guerra VG,Bagnato VS

doi

10.1002/jbio.201800189

subject

Has Abstract

pub_date

2019-04-01 00:00:00

pages

e201800189

issue

4

eissn

1864-063X

issn

1864-0648

journal_volume

12

pub_type

杂志文章
  • Fluorescence hyperspectral imaging (fHSI) using a spectrally resolved detector array.

    abstract::The ability to resolve multiple fluorescent emissions from different biological targets in video rate applications, such as endoscopy and intraoperative imaging, has traditionally been limited by the use of filter-based imaging systems. Hyperspectral imaging (HSI) facilitates the detection of both spatial and spectral...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600304

    authors: Luthman AS,Dumitru S,Quiros-Gonzalez I,Joseph J,Bohndiek SE

    更新日期:2017-06-01 00:00:00

  • Laser exposure of gold nanorods can induce intracellular calcium transients.

    abstract::Uncoated and poly(styrene sulphonate) (PSS)-coated gold nanorods were taken up by NG108-15 neuronal cells. Exposure to 780 nm laser light at the plasmon resonance wavelength of the gold nanorods was found to induce intracellular Ca(2+) transients. The higher Ca(2+) peaks were observed at lower laser doses, with the hi...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201300043

    authors: Paviolo C,Haycock JW,Cadusch PJ,McArthur SL,Stoddart PR

    更新日期:2014-10-01 00:00:00

  • Microfluidics and photonics for Bio-System-on-a-Chip: a review of advancements in technology towards a microfluidic flow cytometry chip.

    abstract::Microfluidics and photonics come together to form a field commonly referred to as 'optofluidics'. Flow cytometry provides the field with a technology base from which both microfluidic and photonic components be developed and integrated into a useful device. This article reviews some of the more recent developments to ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.200810018

    authors: Godin J,Chen CH,Cho SH,Qiao W,Tsai F,Lo YH

    更新日期:2008-10-01 00:00:00

  • Imaging engineered tissues using structural and functional optical coherence tomography.

    abstract::As the field of tissue engineering evolves, there will be an increasingly important need to visualize and track the complex dynamic changes that occur within three-dimensional constructs. Optical coherence tomography (OCT), as an emerging imaging technology applied to biological materials, offers a number of significa...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.200910048

    authors: Liang X,Graf BW,Boppart SA

    更新日期:2009-11-01 00:00:00

  • Biocompatible astaxanthin as novel contrast agent for biomedical imaging.

    abstract::Photoacoustic imaging (PAI) is a hybrid imaging modality with high resolution and sensitivity that can be beneficial for cancer staging. Due to insufficient endogenous photoacoustic (PA) contrast, the development of exogenous agents is critical in targeting cancerous tumors. The current study demonstrates the feasibil...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600159

    authors: Nguyen VP,Park S,Oh J,Wook Kang H

    更新日期:2017-08-01 00:00:00

  • Molecular changes during pollen germination can be monitored by Raman microspectroscopy.

    abstract::The processes associated with pollen germination were studied in vitro for two tree species, Salix caprea and Fraxinus excelsior under different nutrient conditions. The results provide evidence of changes in chemical composition of the pollen grains during germination. From the comparison of spectra of the pollen gra...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201000031

    authors: Schulte F,Panne U,Kneipp J

    更新日期:2010-08-01 00:00:00

  • Pseudo-real-time retinal layer segmentation for high-resolution adaptive optics optical coherence tomography.

    abstract::We present a pseudo-real-time retinal layer segmentation for high-resolution Sensorless Adaptive Optics-Optical Coherence Tomography (SAO-OCT). Our pseudo-real-time segmentation method is based on Dijkstra's algorithm that uses the intensity of pixels and the vertical gradient of the image to find the minimum cost in ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000042

    authors: Janpongsri W,Huang J,Ng R,Wahl DJ,Sarunic MV,Jian Y

    更新日期:2020-08-01 00:00:00

  • Activity assessment of root caries lesions with thermal and near-IR imaging methods.

    abstract::The purpose of this study was to evaluate thermal and near-infrared (NIR) reflectance imaging methods for the assessment of the activity of root caries lesions. In addition, changes in the lesion structure were monitored with polarization sensitive optical coherence tomography (PS-OCT). Artificial bovine and natural r...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201500333

    authors: Lee RC,Darling CL,Staninec M,Ragadio A,Fried D

    更新日期:2017-03-01 00:00:00

  • Intravital microscopy of the lung: minimizing invasiveness.

    abstract::In vivo microscopy has recently become a gold standard in lung immunology studies involving small animals, largely benefiting from the democratization of multiphoton microscopy allowing for deep tissue imaging. This technology represents currently our only way of exploring the lungs and inferring what happens in human...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.201500246

    authors: Fiole D,Tournier JN

    更新日期:2016-09-01 00:00:00

  • Measuring similarity and improving stability in biomarker identification methods applied to Fourier-transform infrared (FTIR) spectroscopy.

    abstract::FTIR spectroscopy is a powerful diagnostic tool that can also derive biochemical signatures of a wide range of cellular materials, such as cytology, histology, live cells, and biofluids. However, while classification is a well-established subject, biomarker identification lacks standards and validation of its methods....

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201300190

    authors: Trevisan J,Park J,Angelov PP,Ahmadzai AA,Gajjar K,Scott AD,Carmichael PL,Martin FL

    更新日期:2014-04-01 00:00:00

  • High fluence light emitting diode-generated red light modulates characteristics associated with skin fibrosis.

    abstract::Skin fibrosis, often referred to as skin scarring, is a significant international health problem with limited treatment options. The hallmarks of skin fibrosis are increased fibroblast proliferation, collagen production, and migration speed. Recently published clinical observations indicate that visible red light may ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600059

    authors: Mamalis A,Koo E,Garcha M,Murphy WJ,Isseroff RR,Jagdeo J

    更新日期:2016-12-01 00:00:00

  • Analysis of light scattering from human breast tissue using a custom dual-optical scanning near-field optical microscope.

    abstract::In this paper we introduce a custom scanning near-field optical microscope (SNOM) that simultaneously collects reflection and transmission near-field images along with topography. This dual-optical SNOM uses a bent probe, which allows for axial reflection imaging, accurate surface scanning, and easy identification of ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201000022

    authors: Kyle JR,Kyle MD,Raghavan R,Budak G,Ozkan CS,Ozkan M

    更新日期:2011-03-01 00:00:00

  • Inverse SORS for detecting a low Raman-active turbid sample placed inside a highly Raman-active diffusely scattering matrix - A feasibility study.

    abstract::The broad range of applications of spatially-offset Raman spectroscopy (SORS) were found to involve samples having only marginal differences in Raman cross-sections between the surface and subsurface targets. We report the results of a feasibility study to evaluate the potential of the approach to identify the presenc...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600075

    authors: Khan KM,Dutta SB,Krishna H,Majumder SK

    更新日期:2016-09-01 00:00:00

  • Application of corneal tomography before keratorefractive procedure for laser vision correction.

    abstract::Ectasia after refractive surgery represents a major concern among refractive surgeons. Corneal abnormalities and preexisting corneal ectasia are the most important risk factors. Corneal topography and central corneal thickness are the factors traditionally screening for in refractive surgery candidates. Study of the a...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201500236

    authors: Luz A,Lopes B,Salomão M,Ambrósio R

    更新日期:2016-05-01 00:00:00

  • Eliminating the scattering ambiguity in multifocal, multimodal, multiphoton imaging systems.

    abstract::In this work we present how to entirely remove the scattering ambiguity present in existing multiphoton multifocal systems. This is achieved through the development and implementation of single-element detection systems that incorporate high-speed photon-counting electronics. These systems can be used to image entire ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201100139

    authors: Hoover EE,Field JJ,Winters DG,Young MD,Chandler EV,Speirs JC,Lapenna JT,Kim SM,Ding SY,Bartels RA,Wang JW,Squier JA

    更新日期:2012-05-01 00:00:00

  • Vibrational spectroscopic imaging and live cell video microscopy for studying differentiation of primary human alveolar epithelial cells.

    abstract::Alveolar type II (ATII) cells in the peripheral human lung spontaneously differentiate toward ATI cells, thus enabling air-blood barrier formation. Here, linear Raman and coherent anti-Stokes Raman scattering (CARS) microscopy are applied to study cell differentiation of freshly isolated ATII cells. The Raman spectra ...

    journal_title:Journal of biophotonics

    pub_type: 社论

    doi:10.1002/jbio.201800052

    authors: Vukosavljevic B,Hittinger M,Hachmeister H,Pilger C,Murgia X,Gepp MM,Gentile L,Huwer H,Schneider-Daum N,Huser T,Lehr CM,Windbergs M

    更新日期:2019-06-01 00:00:00

  • Treatment of tumor in lymph nodes using near-infrared laser light-activated thermosensitive liposome-encapsulated doxorubicin and gold nanorods.

    abstract::Tumor metastasis to lymph nodes is an important contributory factor for cancer-related deaths despite recent developments in cancer therapy. In this study, we demonstrate that tumor in the proper axillary lymph node (PALN) of the mouse can be treated by the application of external laser light to trigger the unloading ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600241

    authors: Matsuki D,Adewale O,Horie S,Okajima J,Komiya A,Oluwafemi O,Maruyama S,Mori S,Kodama T

    更新日期:2017-12-01 00:00:00

  • In vitro photoacoustic spectroscopy of pulsatile blood flow: Probing the interrelationship between red blood cell aggregation and oxygen saturation.

    abstract::We investigate the optical wavelength dependence in quantitative photoacoustic (QPA) assessment of red blood cell (RBC) aggregation and oxygen saturation (sO2 ) during pulsatile blood flow. Experimentally, the pulsatile flow was imaged with a 700 to 900 nm laser using the VevoLAZR. Theoretically, the photoacoustic (PA...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700300

    authors: Bok TH,Hysi E,Kolios MC

    更新日期:2018-08-01 00:00:00

  • The differentiation of oral soft- and hard tissues using laser induced breakdown spectroscopy - a prospect for tissue specific laser surgery.

    abstract::Compared to conventional techniques, Laser surgery procedures provide a number of advantages, but may be associated with an increased risk of iatrogenic damage to important anatomical structures. The type of tissue ablated in the focus spot is unknown. Laser-Induced Breakdown-Spectroscopy (LIBS) has the potential to g...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201600153

    authors: Rohde M,Mehari F,Klämpfl F,Adler W,Neukam FW,Schmidt M,Stelzle F

    更新日期:2017-10-01 00:00:00

  • Potentially malignant lesions of the upper aerodigestive tract.

    abstract::Mucosal lesions of the upper aerodigestive tract usually concern the squamous epithelium of the surface. Many precancerous alterations now designated as potentially malignant are caused by DNA damage resulting from long term exposure to tobacco and alcohol consumption. The distinction from conditions with a similar ph...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.201100085

    authors: Dreyer T,Kreisel M

    更新日期:2012-04-01 00:00:00

  • Comment on "Retinal pulse wave velocity measurement using spectral-domain optical coherence tomography".

    abstract::This paper comments on the article "Retinal pulse wave velocity measurement using spectral-domain optical coherence tomography" by Qian Li et al. The authors propose a method to determine the pulse wave velocity in retinal arteries and veins. This method should enable a noninvasive determination of biomechanical prope...

    journal_title:Journal of biophotonics

    pub_type: 评论,杂志文章

    doi:10.1002/jbio.201700347

    authors: Spahr H,Hillmann D,Pfäffle C,Hüttmann G

    更新日期:2018-02-01 00:00:00

  • Integrated multimodal optical microscopy for structural and functional imaging of engineered and natural skin.

    abstract::An integrated multimodal optical microscope is demonstrated for high-resolution, structural and functional imaging of engineered and natural skin. This microscope incorporates multiple imaging modalities including optical coherence (OCM), multi-photon (MPM), and fluorescence lifetime imaging microscopy (FLIM), enablin...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200003

    authors: Zhao Y,Graf BW,Chaney EJ,Mahmassani Z,Antoniadou E,Devolder R,Kong H,Boppart MD,Boppart SA

    更新日期:2012-05-01 00:00:00

  • Optical coherence elastography for tissue characterization: a review.

    abstract::Optical coherence elastography (OCE) represents the frontier of optical elasticity imaging techniques and focuses on the micro-scale assessment of tissue biomechanics in 3D that is hard to achieve with traditional elastographic methods. Benefit from the advancement of optical coherence tomography, and driven by the in...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.201400108

    authors: Wang S,Larin KV

    更新日期:2015-04-01 00:00:00

  • Analysis of basidiomycete pigments in situ by Raman spectroscopy.

    abstract::Basidiomycetes, that is, mushroom-type fungi, are known to produce pigments in response to environmental impacts. As antioxidants with a high level of unsaturation, these compounds can neutralize highly oxidative species. In the event of close contact with other microbes, the enzymatically controlled pigment productio...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201700369

    authors: Tauber JP,Matthäus C,Lenz C,Hoffmeister D,Popp J

    更新日期:2018-06-01 00:00:00

  • Dynamic imaging and quantification of subcellular motion with eigen-decomposition optical coherence tomography-based variance analysis.

    abstract::The dynamic properties of subcellular organism are important biomarkers of the health. Imaging subcellular level dynamics provides effective solutions for evaluating cell metabolism and testing the responses of cells to pathogens and drugs in pharmaceutical engineering. In this paper, we demonstrate an innovative appr...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201900076

    authors: Wei W,Tang P,Xie Z,Li Y,Wang RK

    更新日期:2019-10-01 00:00:00

  • Determination of the metabolic index using the fluorescence lifetime of free and bound nicotinamide adenine dinucleotide using the phasor approach.

    abstract::The fluorescence lifetime of nicotinamide adenine dinucleotide (NADH) is commonly used in conjunction with the phasor approach as a molecular biomarker to provide information on cellular metabolism of autofluorescence imaging of cells and tissue. However, in the phasor approach, the bound and free lifetime defining th...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201900156

    authors: Ranjit S,Malacrida L,Stakic M,Gratton E

    更新日期:2019-11-01 00:00:00

  • Three-dimensional (3D) backward and forward second harmonic generation (SHG) microscopy of biological tissues.

    abstract::In this work we aim to show how it is possible to exploit the second harmonic generation (SHG) signal for producing multimodal microscopic images of biological tissues. SHG microscopy constitutes an important tool for high-resolution, high-contrast, three-dimensional studies of live cell and tissue architectures. The ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章,评审

    doi:10.1002/jbio.200810060

    authors: Bianchini P,Diaspro A

    更新日期:2008-12-01 00:00:00

  • Assesment of apoptosis induced changes in scattering using optical coherence tomography.

    abstract::The aim of this study is to identify changes in scattering with optical coherence tomography (OCT) and relate these measurements with mitochondrial changes during the initiation of apoptosis. Human retinal pigment epithelial cells were cultured and apoptosis was induced using 10% alcohol. Using the attenuation coeffic...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201500198

    authors: de Bruin DM,Broekgaarden M,van Gemert MJ,Heger M,de la Rosette JJ,Van Leeuwen TG,Faber DJ

    更新日期:2016-09-01 00:00:00

  • N2NSR-OCT: Simultaneous denoising and super-resolution in optical coherence tomography images using semisupervised deep learning.

    abstract::Optical coherence tomography (OCT) imaging shows a significant potential in clinical routines due to its noninvasive property. However, the quality of OCT images is generally limited by inherent speckle noise of OCT imaging and low sampling rate. To obtain high signal-to-noise ratio (SNR) and high-resolution (HR) OCT ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.202000282

    authors: Qiu B,You Y,Huang Z,Meng X,Jiang Z,Zhou C,Liu G,Yang K,Ren Q,Lu Y

    更新日期:2021-01-01 00:00:00

  • Photoacoustically-guided photothermal killing of mosquitoes targeted by nanoparticles.

    abstract::In biomedical applications, nanoparticles have demonstrated the potential to eradicate abnormal cells in small localized pathological zones associated with cancer or infections. Here, we introduce a method for nanotechnology-based photothermal (PT) killing of whole organisms considered harmful to humans or the environ...

    journal_title:Journal of biophotonics

    pub_type: 杂志文章

    doi:10.1002/jbio.201200199

    authors: Foster SR,Galanzha EI,Totten DC,Beneš H,Shmookler Reis RJ,Zharov VP

    更新日期:2014-07-01 00:00:00