Abstract:
:Oysters are important mariculture species worldwide. Because of their filter-feeding behaviors, oysters can accumulate microorganisms, including pathogens, from surrounding water and concentrate bacteria in high numbers. Rapid and suitable methods for quantification of Escherichia coli in oysters are necessary considering that oysters are perishable foods often consumed raw and some countries use E. coli as the regulatory limit. The objective of this study was to develop a qPCR method for quantification of E. coli in oysters. Additionally, different methods were evaluated for DNA extraction from oyster samples and the more reliable method was chosen. Primers and probe were designed targeting uidA gene of E. coli and shown to specifically amplify DNA from E. coli. Standard curves with bacterial DNA extracted from oysters samples artificially inoculated with E. coli were conducted. A good correlation was noticed when the qPCR method was compared to a culture method in oyster samples. This is the first report of a method exclusively developed for direct quantification of E. coli in oyster, the method showed to be suitable for quantification of E. coli in oysters and could be useful in routine analyses, as it requires less time than the culture method.
journal_name
Food Microbioljournal_title
Food microbiologyauthors
Miotto M,Fonseca AA Júnior,Barretta C,da Silva HS,Pellizzaro T,De Dea Lindner J,Vieira CRW,Parveen S,Prudencio ESdoi
10.1016/j.fm.2018.08.015subject
Has Abstractpub_date
2019-02-01 00:00:00pages
85-92eissn
0740-0020issn
1095-9998pii
S0740-0020(18)30241-7journal_volume
77pub_type
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