Abstract:
:Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is a zoonotic pathogen capable of causing severe respiratory disease in humans. Although dromedary camels are considered as a major reservoir host, the MERS-CoV infection dynamics in camels are not fully understood. Through surveillance in Pakistan, nasal (n = 776) and serum (n = 1050) samples were collected from camels between November 2015 and February 2018. Samples were collected from animal markets, free-roaming herds and abattoirs. An in-house ELISA was developed to detect IgG against MERS-CoV. A total of 794 camels were found seropositive for MERS-CoV. Prevalence increased with the age and the highest seroprevalence was recorded in camels aged > 10 years (81.37%) followed by those aged 3.1-10 years (78.65%) and ≤ 3 years (58.19%). Higher prevalence was observed in female (78.13%) as compared to male (70.70%). Of the camel nasal swabs, 22 were found to be positive by RT-qPCR though with high Ct values. Moreover, 2,409 human serum samples were also collected from four provinces of Pakistan during 2016-2017. Among the sampled population, 840 humans were camel herders. Although we found a high rate of MERS-CoV antibody positive dromedaries (75.62%) in Pakistan, no neutralizing antibodies were detected in humans with and without contact to camels.
journal_name
Virol Sinjournal_title
Virologica Sinicaauthors
Zohaib A,Saqib M,Athar MA,Chen J,Sial AU,Khan S,Taj Z,Sadia H,Tahir U,Tayyab MH,Qureshi MA,Mansoor MK,Naeem MA,Hu BJ,Khan BA,Ujjan ID,Li B,Zhang W,Luo Y,Zhu Y,Waruhiu C,Khan I,Yang XL,Sajid MS,Corman VM,doi
10.1007/s12250-018-0051-0subject
Has Abstractpub_date
2018-10-01 00:00:00pages
410-417issue
5eissn
1674-0769issn
1995-820Xpii
10.1007/s12250-018-0051-0journal_volume
33pub_type
杂志文章abstract::HIV-indeterminate Western blotting (WB) results are typically obtained in WB confirmatory assays, and the number of indeterminate samples may increase with the detection of HIV infections, which will present considerable challenges for the management of HIV/AIDS. Nucleic acid detection has been used as a laboratory te...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00130-3
更新日期:2019-08-01 00:00:00
abstract::The virus receptors are key for the viral infection of host cells. Identification of the virus receptors is still challenging at present. Our previous study has shown that human virus receptor proteins have some unique features including high N-glycosylation level, high number of interaction partners and high expressi...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00259-6
更新日期:2020-07-28 00:00:00
abstract::Ebola virus (EBOV) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. Glycoprotein (GP) is the only envelope protein of EBOV, which may play a critical role in virus attachment and entry as well as stimulating host protective immune responses. However, the lack of expression ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-016-3845-y
更新日期:2016-12-01 00:00:00
abstract::Effective oral infection is set off by interaction of a group of conserved per os infectivity factors (PIFs) with larval midgut columnar epithelial cells. We constructed pseudotyped viruses by substituting pif1, pif2 or pif3 genes of Helicoverpa armigera nucleopolyhedrovirus (HearNPV) with their homologs from Mamestra...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0014-5
更新日期:2018-04-01 00:00:00
abstract::Porcine respiratory disease complex (PRDC) is a serious health problem that mainly affects growing and finishing pigs. PRDC is caused by a combination of viral and bacterial agents, such as porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), Mycoplasma hyopneumoniae (Myh), Actinob...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-014-3471-5
更新日期:2014-08-01 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors show clinical promise for the treatment of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the effect of HDAC inhibitor treatment on hepatitis C virus (HCV) replication in Huh7 human liver cells and in a mouse model of HCV infection. Viral replica...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0057-7
更新日期:2018-10-01 00:00:00
abstract::Banana bunchy top virus (BBTV) poses a serious danger to banana crops worldwide. BBTV-encoded protein B4 is a determinant of pathogenicity. However, the relevant molecular mechanisms underlying its effects remain unknown. In this study, we found that a functional peptide could be liberated from protein B4, likely via ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00094-4
更新日期:2019-06-01 00:00:00
abstract::A novel Clade 2.3.2.1c H5N1 reassortant virus caused several outbreaks in wild birds in some regions of China from late 2014 to 2015. Based on the genetic and phylogenetic analyses, the viruses possess a stable gene constellation with a Clade 2.3.2.1c HA, a H9N2-derived PB2 gene and the other six genes of Asian H5N1-o...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-016-3750-4
更新日期:2016-08-01 00:00:00
abstract::During virus infection, RIG-I-like receptors (RLRs) recognize viral RNAs and recruit the adaptor protein VISA to activate downstream signaling, leading to activation of transcription factors NF-κB and IRF3, which collaborate to induce type I interferons (IFNs). IFNs further induce expression of hundreds of IFN-stimula...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00267-6
更新日期:2020-08-07 00:00:00
abstract::A 62-year-old man had chronic hepatitis B virus (HBV) infection and was diagnosed with liver cirrhosis. At the time of diagnosis the patient's virologic markers were positive for hepatitis B surface antigen (HBsAg), antibody to hepatitis B e antigen (anti-HBe) and antibody to hepatitis B core antigen (anti-HBc), while...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3284-3
更新日期:2012-12-01 00:00:00
abstract::Recreational waters contaminated with human fecal pollution are a public health concern, and ensuring the safety of recreational waters for public use is a priority of both the Environmental Protection Agency (EPA) and the Centers for Disease Control and Prevention (CDC). Current recreational water standards rely on f...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-015-3644-x
更新日期:2015-10-01 00:00:00
abstract::Ebola virus (EBOV) and Marburg virus (MARV), belonging to the Filoviridae family, emerged four decades ago and caused severe viral hemorrhagic fever in human and other primates. As high as 50-90% mortality, filoviruses can cause significant threats to public health. However, so far no specific and efficient vaccine ha...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3186-9
更新日期:2011-04-01 00:00:00
abstract::Receptors interaction protein 2 (RIP2) is a specific adaptor molecule in the downstream of NOD2. The role of RIP2 during foot-and-mouth disease virus (FMDV) infection remains unknown. Here, our results showed that RIP2 inhibited FMDV replication and played an important role in the activation of IFN-β and NF-ĸB signal ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00322-2
更新日期:2021-01-05 00:00:00
abstract::Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00109-0
更新日期:2019-08-01 00:00:00
abstract::Bat SARS-like coronavirus (SL-CoV) has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike (S) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have be...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3292-y
更新日期:2013-04-01 00:00:00
abstract::Rotavirus (RV) is a major foodborne pathogen. For RV prevention and control, it is a key to uncover the interaction mechanism between virus and its receptors. However, it is hard to specially purify the viral receptors, including histo-blood group antigens (HBGAs). Previously, the protruding domain protein (P protein)...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00174-5
更新日期:2020-02-01 00:00:00
abstract::Our previous work has shown that Saffold virus (SAFV) induced several rodent and primate cell lines to undergo apoptosis (Xu et al. in Emerg Microb Infect 3:1-8, 2014), but the essential viral proteins of SAFV involved in apoptotic activity lack study. In this study, we individually transfected the viral proteins of S...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00116-1
更新日期:2019-06-01 00:00:00
abstract::Hepatitis C virus (HCV) is a global health concern, notably in Southeast Asia, and in Laos the presentation of the HCV-induced liver disease is poorly known. Our objective was thus to describe a comprehensive HCV infection pattern in order to guide national health policies. A study on a group of 1765 patients formerly...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0039-9
更新日期:2018-08-01 00:00:00
abstract::Chikungunya virus (CHIKV) is a mosquito-borne virus that causes epidemics widely in the world especially in the tropical and subtropical regions. Phylogenetic analysis has found that the CHIKV lineages were associated with the spatial and temporal distributions, which were related to the virus adaption to the major mo...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-017-4077-5
更新日期:2017-12-01 00:00:00
abstract::The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1's infection could repress the basal or inducible transcription ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3213-x
更新日期:2011-12-01 00:00:00
abstract::The protein HTRP (human transcription regulator protein) is encoded by the differential gene htrp and induced by Herpes simplex virus type 1 (HSV-1) infection in KMB-17 cells. HTRP was found to interact with SAP30 (mSin3A Association Protein), one of the components of co-repressor complex mSin3A, which is part of the ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-010-3147-8
更新日期:2010-12-01 00:00:00
abstract::The papain-like protease (PL(pro)) of Middle-East respiratory syndrome coronavirus (MERS-CoV) has proteolytic, deubiquitinating, and deISGylating activities. The latter two are involved in the suppression of the antiviral innate immune response of the host cell. To contribute to an understanding of this process, we pr...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-016-3742-4
更新日期:2016-08-01 00:00:00
abstract::The construction of the first infectious clone JFH-1 speeds up the research on hepatitis C virus (HCV). However, Huh7 cell line was the only highly permissive cell line for HCV infection and only a few clones were fully permissive. In this study, two different fully permissive clones of Huh7 cells, Huh7.5.1 and Huh7-L...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3342-5
更新日期:2013-08-01 00:00:00
abstract::An emerging mosquito-borne arbovirus named Zika virus (ZIKV), of the family Flaviviridae and genus Flavivirus, is becoming a global health threat. ZIKV infection was long neglected due to its sporadic nature and mild symptoms. However, recently, with its rapid spread from Asia to the Americas, affecting more than 30 c...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-016-3780-y
更新日期:2016-04-01 00:00:00
abstract::Isolates of Newcastle disease virus (NDV) from deceased wild and domestic pigeons in Kazakhstan were obtained from the Almaty region during 2005 and were genotypically analyzed by using reverse transcription polymerase chain reaction (RT-PCR) with primers specific to the viral fusion (F) protein gene. Part of the ampl...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3234-0
更新日期:2012-04-01 00:00:00
abstract::Conventional influenza vaccines need to be designed and manufactured yearly. However, they occasionally provide poor protection owing to antigenic mismatch. Hence, there is an urgent need to develop universal vaccines against influenza virus. Using nucleoprotein (NP) and extracellular domain of matrix protein 2 (M2e) ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00138-9
更新日期:2019-10-01 00:00:00
abstract::The aim of the work is the comparison of the epidemiology of influenza and acute respiratory virus infections (ARVI) in the Republic of Kazakhstan with the corresponding influenza epidemic in Russia induced by influenza pandemic virus A/California/07/2009 in 2009. Data on influenza and ARVI from the Republic of Kazakh...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3203-z
更新日期:2011-10-01 00:00:00
abstract::Dengue virus (DENV) and Zika virus (ZIKV) have spread throughout many countries in the developing world and infect millions of people every year, causing severe harm to human health and the economy. Unfortunately, there are few effective vaccines and therapies available against these viruses. Therefore, the discovery ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0068-4
更新日期:2018-12-01 00:00:00
abstract::Newcastle disease virus (NDV) and H9N2 subtype Avian influenza virus (AIV) are two notorious avian respiratory pathogens that cause great losses in the poultry industry. Current inactivated commercial vaccines against NDV and AIV have the disadvantages of inadequate mucosal responses, while an attenuated live vaccine ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00199-1
更新日期:2020-08-01 00:00:00
abstract::Oncolytic alphavirus M1 has been shown to selectively target and kill cancer cells, but cytopathic morphologies induced by M1 virus and the life cycle of the M1 strain in cancer cells remain unclear. Here, we study the key stages of M1 virus infection and replication in the M1 virus-sensitive HepG2 liver cancer cell l...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00339-7
更新日期:2021-01-22 00:00:00