Abstract:
:Salmonella infection is one of the main causes of food-borne diarrheal diseases worldwide. Although most Salmonella infections can be cleared without treatment, some cause serious illnesses that require antibiotic treatment. In view of the growing emergence of antibiotic-resistant Salmonella strains, novel treatments are increasingly required. Furthermore, there is a striking paucity of data on how a balanced human gut microbiota responds to Salmonella infection. This study aimed to evaluate whether a balanced gut microbiota protects against Salmonella growth and to compare two antimicrobial approaches for managing Salmonella infection: bacteriophage (phage) treatment and antibiotic treatment. Anaerobically cultivated human intestinal microflora (ACHIM) is a feasible model for the human gut microbiota and naturally inhibits Salmonella infection. By mimicking Salmonella infection in vitro using ACHIM, we observed a large reduction of Salmonella growth by the ACHIM itself. Treatments with phage and antibiotic further inhibited Salmonella growth. However, phage treatment had less impact on the nontargeted bacteria in ACHIM than the antibiotic treatment did. Phage treatment has high specificity when combating Salmonella infection and offers a noninvasive alternative to antibiotic treatment. IMPORTANCE Antibiotic-resistant bacteria are a global threat. Therefore, alternative approaches for combatting bacteria, especially antibiotic-resistant bacteria, are urgently needed. Using a human gut microbiota model, we demonstrate that bacteriophages (phages) are able to substantially decrease pathogenic Salmonella without perturbing the microbiota. Conversely, antibiotic treatment leads to the eradication of close to all commensal bacteria, leaving only antibiotic-resistant bacteria. An unbalanced microbiota has been linked to many diseases both in the gastrointestinal tract or "nonintestinal" diseases. In our study, we show that the microbiota provides a protective effect against Salmonella. Since phage treatment preserves the healthy gut microbiota, it is a feasible superior alternative to antibiotic treatment. Furthermore, when combating infections caused by pathogenic bacteria, gut microbiota should be considered.
journal_name
mSystemsjournal_title
mSystemsauthors
Hu YOO,Hugerth LW,Bengtsson C,Alisjahbana A,Seifert M,Kamal A,Sjöling Å,Midtvedt T,Norin E,Du J,Engstrand Ldoi
10.1128/mSystems.00119-18subject
Has Abstractpub_date
2018-10-02 00:00:00issue
5issn
2379-5077pii
mSystems00119-18journal_volume
3pub_type
杂志文章相关文献
mSystems文献大全abstract::Sulfur is an essential nutrient that contributes to cellular redox homeostasis, transcriptional regulation, and translation initiation when incorporated into different biomolecules. Transport and reduction of extracellular sulfate followed by cysteine biosynthesis is a major pathway of bacterial sulfur assimilation. F...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00285-19
更新日期:2019-08-06 00:00:00
abstract::Networks encode the interactions between the components in complex systems and play an essential role in understanding complex systems. Microbial ecological networks provide a system-level insight for comprehensively understanding complex microbial interactions, which play important roles in microbial community assemb...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00124-19
更新日期:2019-05-21 00:00:00
abstract::Primary production by Prochlorococcus, the smallest known free-living photosynthetic organism in terms of both physical and genomic size, is thought to have a significant role in global carbon cycles. Despite its small size and low growth rate, Prochlorococcus numerically dominates the phytoplankton community in the n...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00254-18
更新日期:2019-02-26 00:00:00
abstract::In bacterial cells, inhibition of ribosomes by sublethal concentrations of antibiotics leads to a decrease in the growth rate despite an increase in ribosome content. The limitation of ribosomal activity results in an increase in the level of expression from ribosomal promoters; this can deplete the pool of RNA polyme...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00575-20
更新日期:2020-09-08 00:00:00
abstract::GapMind is a Web-based tool for annotating amino acid biosynthesis in bacteria and archaea (http://papers.genomics.lbl.gov/gaps). GapMind incorporates many variant pathways and 130 different reactions, and it analyzes a genome in just 15 s. To avoid error-prone transitive annotations, GapMind relies primarily on a dat...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00291-20
更新日期:2020-06-23 00:00:00
abstract::Biocathodes provide a stable electron source to drive reduction reactions in electrotrophic microbial electrochemical systems. Electroautotrophic biocathode communities may be more robust than monocultures in environmentally relevant settings, but some members are not easily cultivated outside the electrode environmen...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00002-17
更新日期:2017-03-28 00:00:00
abstract::The reactive intermediate deaminase RidA (EC 3.5.99.10) is conserved across all domains of life and deaminates reactive enamine species. When Salmonella entericaridA mutants are grown in minimal medium, 2-aminoacrylate (2AA) accumulates, damages several pyridoxal 5'-phosphate (PLP)-dependent enzymes, and elicits an ob...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00843-19
更新日期:2020-03-10 00:00:00
abstract::Estimations of microbial community diversity based on metagenomic data sets are affected, often to an unknown degree, by biases derived from insufficient coverage and reference database-dependent estimations of diversity. For instance, the completeness of reference databases cannot be generally estimated since it depe...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00039-18
更新日期:2018-04-10 00:00:00
abstract::Simplified microbial communities, or "benchtop microbiomes," enable us to manage the profound complexity of microbial ecosystems. Widespread activities aiming to design and control communities result in novel resources for testing ecological theories and also for realizing new biotechnologies. There is much to be gain...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00106-19
更新日期:2019-05-28 00:00:00
abstract::Gut microbial β-glucuronidase (GUS) enzymes play important roles in drug efficacy and toxicity, intestinal carcinogenesis, and mammalian-microbial symbiosis. Recently, the first catalog of human gut GUS proteins was provided for the Human Microbiome Project stool sample database and revealed 279 unique GUS enzymes org...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00452-19
更新日期:2019-08-27 00:00:00
abstract::Bumble bees are important pollinators in natural and agricultural ecosystems. Their social colonies are founded by individual queens, which, as the predominant reproductive females of colonies, contribute to colony function through worker production and fitness through male and new queen production. Therefore, queen h...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00631-19
更新日期:2019-12-10 00:00:00
abstract::Quantitative understanding and prediction of microbial community dynamics are an outstanding challenge. We test the hypothesis that metabolic mechanisms provide a foundation for accurate prediction of dynamics in microbial systems. In our research, metabolic models have been able to accurately predict species interact...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00146-19
更新日期:2019-06-11 00:00:00
abstract::High-nucleic-acid (HNA) and low-nucleic-acid (LNA) bacteria are two operational groups identified by flow cytometry (FCM) in aquatic systems. A number of reports have shown that HNA cell density correlates strongly with heterotrophic production, while LNA cell density does not. However, which taxa are specifically ass...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00093-19
更新日期:2019-09-10 00:00:00
abstract::The intestinal microbiota contains trillions of commensal microorganisms that shape multiple aspects of host physiology and disease. In contrast to the host's genome, the microbiome is amenable to change over the course of an organism's lifetime, providing an opportunity to therapeutically modulate the microbiome's im...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00216-18
更新日期:2019-02-19 00:00:00
abstract::Health-associated oral Streptococcus species are promising probiotic candidates to protect against dental caries. Ammonia production through the arginine deiminase system (ADS), which can increase the pH of oral biofilms, and direct antagonism of caries-associated bacterial species are desirable properties for oral pr...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00158-18
更新日期:2018-12-18 00:00:00
abstract::Microbiology, like many areas of life science research, is increasingly data-intensive. As such, bioinformatics and data science skills have become essential to leverage microbiome sequencing data for discovery. Short intensive courses have sprung up as formal computational training opportunities at individual institu...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00467-19
更新日期:2019-08-20 00:00:00
abstract::Single mutations frequently alter several aspects of cell behavior but rarely reveal whether a particular statistically significant change is biologically significant. To determine which behavioral changes are most important for multicellular self-organization, we devised a new methodology using Myxococcus xanthus as ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00518-20
更新日期:2020-07-14 00:00:00
abstract::The 2,3-seco pathway, the pathway for anaerobic cholesterol degradation, has been established in the denitrifying betaproteobacterium Sterolibacterium denitrificans. However, knowledge of how microorganisms respond to cholesterol at the community level is elusive. Here, we applied mesocosm incubation and 16S rRNA sequ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00113-18
更新日期:2018-10-30 00:00:00
abstract::Recent studies of mammalian microbiomes have identified strong phylogenetic effects on bacterial community composition. Bats (Mammalia: Chiroptera) are among the most speciose mammals on the planet and the only mammal capable of true flight. We examined 1,236 16S rRNA amplicon libraries of the gut, oral, and skin micr...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00511-19
更新日期:2019-11-12 00:00:00
abstract::A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc2155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analy...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00534-20
更新日期:2020-10-06 00:00:00
abstract::Methylobacter species, members of the Methylococcales, have recently emerged as some of the globally widespread, cosmopolitan species that play a key role in the environmental consumption of methane across gradients of dioxygen tensions. In this work, we approached the question of how Methylobacter copes with hypoxia,...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00770-19
更新日期:2020-01-21 00:00:00
abstract::Given the complexity of host-microbiota symbioses, scientists and philosophers are asking questions at new biological levels of hierarchical organization-what is a holobiont and hologenome? When should this vocabulary be applied? Are these concepts a null hypothesis for host-microbe systems or limited to a certain spe...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00028-16
更新日期:2016-03-29 00:00:00
abstract::Biological sulfur cycling in polar, low-temperature ecosystems is an understudied phenomenon in part due to difficulty of access and the dynamic nature of glacial environments. One such environment where sulfur cycling is known to play an important role in microbial metabolisms is located at Borup Fiord Pass (BFP) in ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00504-20
更新日期:2020-08-04 00:00:00
abstract::Intestinal bacterial dysbiosis has been increasingly linked to ankylosing spondylitis (AS), which is a prototypic and best studied subtype of spondyloarthritis (SpA). Fungi and bacteria coexist in the human gut and interact with each other. Although they have been shown to contribute actively to health or disease, no ...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00176-18
更新日期:2019-03-26 00:00:00
abstract::Using automated genome analysis tools, it is often unclear to what degree genetic variability in homologous biosynthetic pathways relates to structural variation. This hampers strain prioritization and compound identification and can lead to overinterpretation of chemical diversity. Here, we assessed the metabolic pot...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00125-20
更新日期:2020-06-02 00:00:00
abstract::Fecal microbiota transplantation (FMT) is a proposedly useful strategy for the treatment of gastrointestinal (GI) disorders through remediation of the patient gut microbiota. However, its therapeutic success has been variable, necessitating research to uncover mechanisms that improve patient response. Antibiotic pretr...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00404-19
更新日期:2020-01-28 00:00:00
abstract::Marine oil spills can impact both coastal and offshore marine environments, but little information is available on how the microbial response to oil and dispersants might differ between these biomes. Here, we describe the compositional and functional response of microbial communities to different concentrations of oil...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00668-20
更新日期:2020-08-25 00:00:00
abstract::The gut microbiota has been implicated in immunoglobin A nephropathy (IgAN) because the intestinal immune response is assumed to be one of the disease triggers. Since the microbial composition is heritable, we hypothesize that genetic variants controlling gut microbiota composition may be associated with susceptibilit...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00819-20
更新日期:2021-01-12 00:00:00
abstract::Vitamin B12 is synthesized by prokaryotes in the rumens of dairy cows-and this has implications in human nutrition since humans rely on consumption of dairy products for vitamin B12 acquisition. However, the concentration of vitamin B12 in milk is highly variable, and there is interest in determining what causes vitam...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00107-20
更新日期:2020-03-03 00:00:00
abstract::Increasing rates of antibiotic-resistant bacterial infection are one of the most pressing contemporary global health concerns. The ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) have been identified as the...
journal_title:mSystems
pub_type: 杂志文章
doi:10.1128/mSystems.00024-18
更新日期:2018-04-17 00:00:00