Exosomes derived from miR-146a-modified adipose-derived stem cells attenuate acute myocardial infarction-induced myocardial damage via downregulation of early growth response factor 1.

Abstract:

:Multiple studies demonstrated that early growth response factor 1 (EGR1) induces myocardial damage after acute myocardial infarction (AMI). Recent evidence indicates that microRNAs (miRNA) play an important role in exosome-mediated cardioprotection after AMI. Bioinformatics analysis has shown that miR-146a can regulate the expression of EGR1, so the aim of this study was to determine if miR-146a plays a role in exosome-mediated cardioprotection by regulation of EGR1 after AMI. Exosomes were isolated from wild- or miR-146a-modified adipose-derived stem cells (ADSCs), and the therapeutic effect of exosomes was assessed in an AMI model in rats and hypoxic-induced H9c2 model cells. The results showed that miR-146a containing exosomes had more effect than the exosome treatment group on the suppression of AMI-induced apoptosis, inflammatory response, and fibrosis in an AMI rat model. Both in vivo and in vitro experiments found that miR-146a interacted with the 3'-untranslated region of EGR1 and suppressed posttranscriptional EGR1 expression, which was confirmed by the luciferase reporter assay. We also found that suppressed EGR1 expression reversed AMI or hypoxia-induced TLR4/NFκB signal activation, which played an important role in the promotion of myocardial cell apoptosis, inflammatory response, and fibrosis. Taken together, these findings suggested that exosomes derived from miR-146a-modified ADSCs attenuated AMI-induced myocardial damage via downregulation of EGR1.

journal_name

J Cell Biochem

authors

Pan J,Alimujiang M,Chen Q,Shi H,Luo X

doi

10.1002/jcb.27731

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

4433-4443

issue

3

eissn

0730-2312

issn

1097-4644

journal_volume

120

pub_type

杂志文章
  • Inhibition of osteoblastic cell differentiation by conditioned medium derived from the human prostatic cancer cell line PC-3 in vitro.

    abstract::Human prostatic carcinoma frequently metastasizes to bone tissue and activates bone metabolism, especially bone formation, at the site of metastasis. It has been reported that an extract of prostatic carcinoma and conditioned medium (CM) of a human prostatic carcinoma cell line, PC-3, established from a bone metastast...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Kido J,Yamauchi N,Ohishi K,Kataoka M,Nishikawa S,Nakamura T,Kadono H,Ikedo D,Ueno A,Nonomura N,Okuyama A,Nagata T

    更新日期:1997-11-01 00:00:00

  • E-, P-, and N-cadherin are co-expressed in the nasopharyngeal carcinoma cell line TW-039.

    abstract::The cadherin/catenin complex plays a key role in the initiation of cell-cell recognition, and adhesion, and the elaboration of structural and functional organization in multicellular tissues and organs. It is associated with tumor metastasis and also acts as an "invasion suppressor" of cancer cells. Nasopharyngeal car...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Lou PJ,Chen WP,Lin CT,DePhilip RM,Wu JC

    更新日期:1999-11-01 00:00:00

  • T-box 3 negatively regulates osteoblast differentiation by inhibiting expression of osterix and runx2.

    abstract::T-box (Tbx)3, a known transcriptional repressor, is a member of a family of transcription factors, which contain a highly homologous DNA binding domain known as the Tbx domain. Based on the knowledge that mutation of the Tbx3 gene results in limb malformation, Tbx3 regulates osteoblast proliferation and its expression...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22035

    authors: Govoni KE,Linares GR,Chen ST,Pourteymoor S,Mohan S

    更新日期:2009-02-15 00:00:00

  • Human cardiomyocytes express high level of Na+/glucose cotransporter 1 (SGLT1).

    abstract::We have quantitatively measured gene expression for the sodium-dependent glucose cotransporters 1 and 2 (SGLT1 and SGLT2) in 23 human tissues using the method of real time PCR. As predicted, our results revealed that the expression of SGLT1 was very high in the small intestine (1.2E + 6 molecules/microg total RNA) rel...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10631

    authors: Zhou L,Cryan EV,D'Andrea MR,Belkowski S,Conway BR,Demarest KT

    更新日期:2003-10-01 00:00:00

  • 3,4-Dimethoxystilbene, a resveratrol derivative with anti-angiogenic effect, induces both macroautophagy and apoptosis in endothelial cells.

    abstract::Angiogenesis plays an important role in many pathological processes. Identification of novel anti-angiogenic agents will provide new insights into the mechanisms for angiogenesis as well as potential lead compounds for developing new drugs. In the present study, a series of resveratrol methylated derivatives have been...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24411

    authors: Zhang L,Jing H,Cui L,Li H,Zhou B,Zhou G,Dai F

    更新日期:2013-03-01 00:00:00

  • Smad8/9 Is Regulated Through the BMP Pathway.

    abstract::Members of the transforming growth factor-β (TGF-β) family function through Smad-dependent and Smad-independent pathways. The Smad-dependent pathway is stimulated through the phosphorylation of receptor-regulated Smad (R-Smad) and inhibited through the dephosphorylation of R-Smad or the gene induction of inhibitory Sm...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25478

    authors: Katakawa Y,Funaba M,Murakami M

    更新日期:2016-08-01 00:00:00

  • Recent Advances in Mechanobiology of Osteosarcoma.

    abstract::Mechanotransduction is a key process by which cells perceive extracellular mechanical cues/intercellular physical interactions and transform them into intracellular biochemical signals. This physiological process is crucial during bone development and bone remodeling throughout childhood and adult life, whereas severa...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.25660

    authors: Adamopoulos C,Gargalionis AN,Piperi C,Papavassiliou AG

    更新日期:2017-02-01 00:00:00

  • Inhibitory effect of uremic solutions on protein-DNA-complex formation of the vitamin D receptor and other members of the nuclear receptor superfamily.

    abstract::Chronic renal failure is often associated with a resistance to the biologically active form of vitamin D(3), the nuclear hormone 1alpha, 25-dihydroxyvitamin D(3) (VD). The actions of VD are mediated by the vitamin D receptor (VDR), a ligand-dependent transcription factor that binds as a dimeric complex with the retino...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19990901)74:3<386::aid-jcb

    authors: Toell A,Degenhardt S,Grabensee B,Carlberg C

    更新日期:1999-09-01 00:00:00

  • CDK2 destabilizes tumor suppressor C/EBPα expression through ubiquitin-mediated proteasome degradation in acute myeloid leukemia.

    abstract::Deregulation and functional inhibition of CCAAT-enhancer-binding protein α (C/EBPα), a key transcription factor of myeloid lineage leads to development of myeloid leukemia. In this study, we show that cyclin-dependent kinase 2 (CDK2) negatively regulates C/EBPα protein levels in myeloid leukemia cells. The overexpress...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29516

    authors: Thacker G,Mishra M,Sharma A,Singh AK,Sanyal S,Trivedi AK

    更新日期:2020-04-01 00:00:00

  • Signal transducer and activator of transcription 3 pathway mediates genipin-induced apoptosis in U266 multiple myeloma cells.

    abstract::It has drawn a lot of attention to target signal transducer and activator of transcription 3 (STAT3) as a potential strategy for cancer therapeutics. Using several myelogenous cell lines, the effect of genipin (an active compound of Gardenia fruit) on the STAT3 pathway and apoptosis was investigated. Genipin suppresse...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23077

    authors: Lee JC,Ahn KS,Jeong SJ,Jung JH,Kwon TR,Rhee YH,Kim SH,Kim SY,Yoon HJ,Zhu S,Chen CY,Kim SH

    更新日期:2011-06-01 00:00:00

  • Epigenetic modifications, chromatin distribution and TP53 transcription in a model of breast cancer progression.

    abstract::In the present paper we aimed to characterize epigenetic aspects and analyze TP53 transcription in the 21 T series, composed of breast cell lines: non-cancerous H16N2; Atypical Ductal Hyperplasia 21PT; Ductal Carcinoma in situ 21NT and Invasive Metastatic Carcinoma 21MT1. We detected a global genomic hypomethylation i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25003

    authors: Santos GC Jr,da Silva AP,Feldman L,Ventura GM,Vassetzky Y,de Moura Gallo CV

    更新日期:2015-04-01 00:00:00

  • SRrp37, a novel splicing regulator located in the nuclear speckles and nucleoli, interacts with SC35 and modulates alternative pre-mRNA splicing in vivo.

    abstract::We report here the identification and characterization of a novel SR-related protein, referred to as SRrp37, based on its apparent molecular weight and subcellular location. SRrp37 was identified through a yeast two-hybrid screen during the course of searching for proteins interacting with pNO40, a ribosomal 60S core ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22255

    authors: Ouyang P

    更新日期:2009-09-01 00:00:00

  • Endothelial cell fatty acid unsaturation mediates cold-induced oxidative stress.

    abstract::Ultraprofound hypothermia (< 5 degrees C) induces changes to cell membranes such as liquid-to-gel lipid transitions and oxidative stress that have a negative effect on membrane function and cell survival. We hypothesized that fatty acid substitution of endothelial cell lipids and alterations in their unsaturation woul...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20961

    authors: Zieger MA,Gupta MP,Siddiqui RA

    更新日期:2006-10-15 00:00:00

  • Amino-terminus domain of the androgen receptor as a molecular target to prevent the hormonal progression of prostate cancer.

    abstract::Prostate cancer has a propensity to metastasize to the bone. Currently the only effective systemic treatment for these patients is androgen ablation therapy. However, the tumor will invariably progress to an androgen-independent stage and the patient will succumb to his disease within approximately 2 years. The earlie...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.20802

    authors: Wang G,Sadar MD

    更新日期:2006-05-01 00:00:00

  • Fenofibrate improves renal function by amelioration of NOX-4, IL-18, and p53 expression in an experimental model of diabetic nephropathy.

    abstract::Among several pathological mechanisms involved in diabetic nephropathy, oxidative stress, inflammation, and apoptosis play a prominent role. Fenofibrate, a peroxisome proliferator-activated receptor-α (PPAR-α) agonist, has markedly improved oxidative stress and inflammatory responses, but there is no evidence about it...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27055

    authors: Yaribeygi H,Mohammadi MT,Rezaee R,Sahebkar A

    更新日期:2018-09-01 00:00:00

  • Immunological comparison of desmosomal components from several bovine tissues.

    abstract::A panel of monoclonal antibodies and conventional antisera directed against desmosomal proteins from bovine muzzle epidermis was used to identify immunologically related proteins from two other bovine stratified squamous epithelia, cornea and esophagus. Desmosome-enriched tissue fractions were prepared from epidermis,...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240260104

    authors: Giudice GJ,Cohen SM,Patel NH,Steinberg MS

    更新日期:1984-01-01 00:00:00

  • MicroRNA-9 inhibits retinal neovascularization in rats with diabetic retinopathy by targeting vascular endothelial growth factor A.

    abstract::Diabetic retinopathy (DR) is a leading cause of adult visual impairment and loss. This study aims to explore the effects of microRNA-9 (miR-9) on retinal neovascularization during DR by targeting the vascular endothelial growth factor A (VEGFA). DR rat models were successfully established. Retinal microvascular endoth...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.28081

    authors: Liu WL

    更新日期:2018-11-28 00:00:00

  • miR-381-3p restrains cervical cancer progression by downregulating FGF7.

    abstract::This study aimed at elucidating the molecular mechanism of miR-381-3p in cervical cancer progression, which may provide a novel therapeutic target for patients with cervical cancer. The expression of miR-381-3p was confirmed by quantitative reverse transcription polymerase chain reaction. Microarray analysis was condu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27438

    authors: Shang A,Zhou C,Bian G,Chen W,Lu W,Wang W,Li D

    更新日期:2019-01-01 00:00:00

  • Integrative genomics: liver regeneration and hepatocellular carcinoma.

    abstract::Numerous genome wide profiles of gene expression changes in human hepatocellular carcinoma (HCC), compared to normal liver tissue, have been reported. Hierarchical clustering of these data reveal distinct patterns, which underscore conservation between human disease and mouse models of HCC, as well as suggest specific...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.24104

    authors: Coban Z,Barton MC

    更新日期:2012-07-01 00:00:00

  • MPA increases idarubicin-induced apoptosis in chronic lymphatic leukaemia cells via caspase-3.

    abstract::The caspase family of protease is speculated to have a crucial role in apoptosis. The effect of treatment with Idarubicin (IDA) and Medroxyprogesterone acetate (MPA), used alone or in combination, on the activation of Caspase-3 in canine Chronic Lymphatic Leukaemia (CLL) cells was investigated, in order to clarify the...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10556

    authors: Florio S,Crispino L,Ciarcia R,Vacca G,Pagnini U,de Matteis A,Pacilio C,D'Andrilli G,Kumar C,Giordano A

    更新日期:2003-07-01 00:00:00

  • 1α,25(OH)2D3-dependent modulation of Akt in proliferating and differentiating C2C12 skeletal muscle cells.

    abstract::We previously reported that 1α,25-dihydroxy-vitamin D(3) [1α,25(OH)(2)D(3)] induces non-transcriptional rapid responses through activation of Src and MAPKs in the skeletal muscle cell line C2C12. In the present study we investigated the modulation of Akt by the secosteroid hormone in C2C12 cells at proliferative stage...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.23444

    authors: Buitrago CG,Arango NS,Boland RL

    更新日期:2012-04-01 00:00:00

  • Protective effects of microRNA-330 on amyloid β-protein production, oxidative stress, and mitochondrial dysfunction in Alzheimer's disease by targeting VAV1 via the MAPK signaling pathway.

    abstract::This study aims to explore the effect of miR-330 targeting VAV1 on amyloid β-protein (Aβ) production, oxidative stress (OS), and mitochondrial dysfunction in Alzheimer's disease (AD) mice through the MAPK signaling pathway. Putative targeted gene of miR-330 was performed by a miRNA target prediction website and dual-l...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26700

    authors: Zhou Y,Wang ZF,Li W,Hong H,Chen J,Tian Y,Liu ZY

    更新日期:2018-07-01 00:00:00

  • miR-200a mediates protection of thymosin β-4 in cardiac microvascular endothelial cells as a novel mechanism under hypoxia-reoxygenation injury.

    abstract::Thymosin β-4 (Tβ4) is a ubiquitous protein, which has been suggested to regulate multiple cell signal pathways and a variety of cellular functions. However, the role Tβ4 plays in the cardiac microvascular endothelial cells (CMECs) under myocardial ischemia/reperfusion injury is currently unknown. Here we investigated ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29237

    authors: Li Y,Zhu X,Liu X,Du A,Yu B

    更新日期:2019-11-01 00:00:00

  • The control of fracture healing and its therapeutic targeting: improving upon nature.

    abstract::Fracture repair is a complex process involving timed cellular recruitment, gene expression, and synthesis of compounds that regenerate native tissue to restore the mechanical integrity, and thus function of injured bone. While the majority of fractures heal without complication, this takes time and a subset of patient...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.22418

    authors: Komatsu DE,Warden SJ

    更新日期:2010-02-01 00:00:00

  • Association of vanadate-sensitive Mg(2+)-ATPase and shape change in intact red blood cells.

    abstract::Intact human erythrocytes, initially depleted of Mg2+ by EDTA incubation in the presence of A23187, exhibit Mg(2+)-dependent phosphate production of around 1.5 mmol per liter cells.h, half-maximally activated at around 0.4 mM added free Mg2+. This appears to correspond to Mg(2+)-stimulated adenosine triphosphatase (Mg...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240460403

    authors: Xu YH,Lu ZY,Conigrave AD,Auland ME,Roufogalis BD

    更新日期:1991-08-01 00:00:00

  • ECM-Dependence of Endothelial Progenitor Cell Features.

    abstract::Preserving self-renewal, multipotent capacity, and large-scale expansion of highly functional progenitor cells, including endothelial progenitor cells (EPCs), is a controversial issue. These current limitations, therefore, raise the need of developing promising in vitro conditions for prolonged expansion of EPCs witho...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25492

    authors: Siavashi V,Nassiri SM,Rahbarghazi R,Vafaei R,Sariri R

    更新日期:2016-08-01 00:00:00

  • Neurogenic Differentiation of Mesenchymal Stem Cells Induces Alterations in Extracellular Nucleotides Metabolism.

    abstract::The presented results show for the first time that the neurogenic transdifferentiation of hUC-MSCs considerably changes the elements of purinergic signaling profile. Although, it has been demonstrated in the literature that extracellular nucleotides and nucleosides determine the fate of mesenchymal and neural stem cel...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25664

    authors: Czarnecka J,Porowińska D,Bajek A,Hołysz M,Roszek K

    更新日期:2017-03-01 00:00:00

  • Inhibitory helix-loop-helix transcription factors Id1/Id3 promote bone formation in vivo.

    abstract::Bone formation is under the control of a set of transcription factors. Ids are inhibitory helix-loop-helix (HLH) transcription factors and expression of Id genes in osteoblasts is under the control of calciotropic agents such as BMP and vitamin D. However, the function of Ids during bone formation in vivo has not yet ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20154

    authors: Maeda Y,Tsuji K,Nifuji A,Noda M

    更新日期:2004-10-01 00:00:00

  • Calmodulin binding to the Fas-mediated death-inducing signaling complex in cholangiocarcinoma cells.

    abstract::We have previously demonstrated that the antagonists of calmodulin (CaM) induce apoptosis of cholangiocarcinoma cells partially through Fas-mediated apoptosis pathways. Recently, CaM has been shown to bind to Fas, which is regulated during Fas or CaM antagonist-mediated apoptosis in Jurkat cells and osteoclasts. Accor...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21447

    authors: Chen Y,Pawar P,Pan G,Ma L,Liu H,McDonald JM

    更新日期:2008-02-15 00:00:00

  • Role of the Helicobacter pylori-induced inflammatory response in the development of gastric cancer.

    abstract::Helicobacter pylori (H. pylori) infection causes chronic gastritis and peptic ulceration and is the strongest risk factor for the development of gastric cancer. The pathogenesis of H. pylori is believed to be associated with infection-initiated chronic gastritis, which is characterized by enhanced expression of many i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.24389

    authors: Lamb A,Chen LF

    更新日期:2013-03-01 00:00:00