Abstract:
:The use of corneal tissue for ex vivo therapeutic evaluations is limited due to its rapid loss of viability after excision. Optimization of storage conditions may allow prolonged retention of physical tissue properties. In this study, we evaluated how storage in optimized organ culture (OC) medium at 37°C or phosphate-buffered saline (PBS) at 2-8°C impacted physical properties of bovine corneas. Tissue hydration, permeability and histology were monitored at baseline and following 1, 4 and 7 days of storage. Corneas stored in OC demonstrated significantly higher hydration and permeability when compared to those stored in PBS. Histology revealed that storage in OC consistently caused detachment of the epithelial layer by day 4 of storage, whereas both storage conditions caused a significant increase in stromal thickness and tissue vacuolation. This study highlights the limitations of currently available corneal tissue storage approaches for ex vivo drug permeation studies.
journal_name
Ophthalmic Resjournal_title
Ophthalmic researchauthors
Thakur SS,Shrestha D,Rupenthal IDdoi
10.1159/000493488subject
Has Abstractpub_date
2019-01-01 00:00:00pages
204-209issue
4eissn
0030-3747issn
1423-0259pii
000493488journal_volume
61pub_type
杂志文章abstract:OBJECTIVE:To assess changes of tear film function in patients with pterygium after pterygium excision combined with limbal-conjunctival autograft transplantation. METHODS:Sixty eyes of 60 patients were entered in the study. The Schirmer test, tear breakup time (BUT) and mucus fern test (MFT) were evaluated in the pati...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000321531
更新日期:2011-01-01 00:00:00
abstract::The purpose of this study was to determine the expression of metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in the aqueous humour of patients with exfoliation syndrome (XFS). XFS and control samples were analysed for their MMP content by zymography and for their tissue inhibitors by ELISA. In XFS eyes, ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000063661
更新日期:2002-05-01 00:00:00
abstract:PURPOSE:The aim of this study was to evaluate the choroidal thickness (CT) and its determinants in primary angle closure glaucoma (PACG) eyes with and without malignant glaucoma (MG). METHODS:In this cross-sectional study, we enrolled 25 patients with bilateral primary angle closure (PAC) diseases; 1 eye of each patie...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000490914
更新日期:2018-01-01 00:00:00
abstract::The ophthalmic examination of patients with hypertrophic cardiomyopathy treated with high doses of verapamil over a considerable period of time showed no evidence of a side effect of the drug, thus proving its ocular safety. This could be verified by densitometric image analysis of Scheimpflug photographs of the eyes....
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265327
更新日期:1984-01-01 00:00:00
abstract::The eyes of 200 rats (Mol:SPRD, Moellegaard Ltd., Skensvet, Denmark), 8 weeks of age and of both sexes, were examined routinely with a photo-slitlamp microscope (Zeiss) and opthalmoscope (Heine) 3-4 times in the course of four different 12-week toxicity studies. The animals were kept under constant lighting conditions...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267492
更新日期:1994-01-01 00:00:00
abstract::The turnover and migration of synthesized glycoproteins in the cultured retinal pigment epithelium have been studied. Quantitative study indicated that half-time of 3H-fucosylglycoproteins was 11.6 days. Autoradiography was performed several times after incubation of 3H-fucose. Retinal pigment epithelium constantly sy...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265423
更新日期:1986-01-01 00:00:00
abstract:AIMS:To evaluate the biologic effects of fluoroquinolone eye solutions without preservatives on cultured human corneal epithelial cells in vitro. METHODS:We studied the effect of diverse generations of topical fluoroquinolones such as ofloxacin 0.3%, levofloxacin 0.5%, tosufloxacin 0.3%, moxifloxacin 0.5% and gatiflox...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000357976
更新日期:2014-01-01 00:00:00
abstract::Previous research from this laboratory showed that human lacrimal fluid peroxidase has cyclic variations during the menstrual cycle, correlated with plasma levels of 17beta-oestradiol. In the present investigation, variations of enzyme activity and total protein content during the oestrous cycle of young adult female ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055666
更新日期:2001-05-01 00:00:00
abstract:INTRODUCTION:Retinitis pigmentosa (RP) is an inherited retinal disorder, characterized by photoreceptor degeneration inducing progressive vision loss. This study evaluates its impact on quality of life (QOL) and emotional states of patients affected by RP. METHODS:A cross-sectional study was conducted on 60 RP patient...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000435886
更新日期:2015-01-01 00:00:00
abstract::To evaluate the effect of single or repeated topical applications of prostaglandin E2 (PGE2) to the cornea on the blood-aqueous barrier, we have measured the extent of flare in the anterior chamber of rabbit eyes at various time intervals. PGE2 was applied to the cornea for 4 min with the use of a glass cylinder in pi...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267895
更新日期:1996-01-01 00:00:00
abstract::Dry eye is the most prominent pathology among those involving the ocular surface: a decrease of the aqueous (less frequent) or the lipid (more frequent) component of the tear film is the cause of the diminished stability of tears that is observed in this pathology. Dry eye shows a clear distribution linked to both sex...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000489093
更新日期:2018-01-01 00:00:00
abstract:PURPOSE:To analyze a multiflash multifocal electroretinogram in 20 patients with open-angle glaucoma (OAG). METHODS:The stimulation sequence consisted of a binary m-sequence (L(max) 200 cd/m(2), L(min) <1 cd/m(2)). Each m-sequence stimulus was followed by three global flashes (luminance: 400 cd/m(2)) at an interval of...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000048333
更新日期:2002-03-01 00:00:00
abstract::The rate of reepithelialization of the rabbit cornea, receiving isotonic NaCl solution and acetylcysteine (Mucomyst) solution at 10 and 20% concentrations, following a superficial epithelial ulcer was studied by fluorescein staining, photography and light microscopy. Acetylcysteine solution does not seem to retard nor...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265198
更新日期:1982-01-01 00:00:00
abstract::HY-1 genotype chick lenses display epithelial hyperplasia and associated lens opacities. Membrane proteins from HY-1 and normal chick lenses were separated by isoelectric focusing, and 45Ca2+ binding capacities were determined. Markedly higher (congruent to 900-fold) binding of 45Ca2+ was observed in high molecular we...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265397
更新日期:1985-01-01 00:00:00
abstract::Equine recurrent uveitis (ERU) is an autoimmune disease that occurs with a high prevalence (10%) in horses. ERU represents the only reliable spontaneous model for human autoimmune uveitis. We already identified and characterized novel autoantigens (malate dehydrogenase, recoverin, CRALBP) by analyzing the autoantibody...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000119867
更新日期:2008-01-01 00:00:00
abstract::We examined the density and morphology of lens epithelial cells (LECs) in vivo in a group of normal volunteers and cataract patients by using a newly developed noncontact specular microscope. There was a statistically significant decrease in the cell density of LECs in a group of cataract patients over the age of 80 y...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055694
更新日期:2001-11-01 00:00:00
abstract:PURPOSE:The purpose of our study was to investigate the profiles of inflammatory cytokines and the macrophage polarization gene in a choroidal neovascularization (CNV) mouse model before and after intravitreal aflibercept treatment. METHODS:The CNV mouse model was conducted by laser photocoagulation. A total of 58 cyt...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000513588
更新日期:2020-12-04 00:00:00
abstract:PURPOSE:The possibility of providing cultured corneal endothelial cells (CECs) for clinical transplantation has gained much attention. However, the worldwide need for human (h) donor corneas far exceeds supply. The pig (p) might provide an alternative source. The aim of this study was to compare the proliferative capac...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000342978
更新日期:2013-01-01 00:00:00
abstract::Toxoplasma gondii is an extremely successful opportunistic parasite which infects approximately one third of the human population worldwide. The impact of this parasite on human health becomes particularly manifest in congenital damage with infection and subsequent inflammation of neuronal tissues including the retina...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000363141
更新日期:2014-01-01 00:00:00
abstract::Inflammation is substantially contributing to the development and worsening of diabetic retinopathy in general and diabetic macular edema (DME) in particular, which provides the rationale to treat DME with corticosteroids. While anti-vascular endothelial growth factor (VEGF) agents are mostly chosen as a first-line tr...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000499540
更新日期:2019-01-01 00:00:00
abstract::We studied lens opacification of rabbit lenses in culture. Opacification was produced by maintaining rabbit lenses in medium containing calcium concentrations as high as 10 mmol/l and the opacity was measured using LASER spectroscopy. Increasing calcium caused significant opacification in lens cortex while the transpa...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265380
更新日期:1985-01-01 00:00:00
abstract::Given the propensity of a large number of melanogenic pathways that can be modulated by cellular redox status, a causal role of the deficiency of ocular pigments such as melanin in the pathogenesis of age-related macular degeneration and evidence that melanin production does occur in the adult eye, it seems not improb...
journal_title:Ophthalmic research
pub_type: 杂志文章,评审
doi:10.1159/000081632
更新日期:2004-11-01 00:00:00
abstract:PURPOSE:To assess and describe sequential morphological changes in the choroidal neovascularization (CNV) net using optical coherence tomography angiography (OCTA) in patients undergoing treatment with intravitreal antivascular endothelial growth factor (VEGF). METHODS:Prospective cohort study. OCTA was performed sequ...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000442671
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND/AIMS:To evaluate the safety of suramin compared with mitomycin-C (MMC) as an adjunctive agent in trabeculectomy by determining its ciliary body toxicity at predetermined effective dosages in rabbit eyes. METHODS:Thirty-two New Zealand albino rabbits received either suramin (200, 300, 400, or 800 mg/ml) or M...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000187625
更新日期:2009-01-01 00:00:00
abstract::In order to develop a more adequate ultraviolet (UV)-induced cataract model, a combination of l-buthionine sulfoximine (BSO) treatment was applied to experimental animals. A single administration of BSO 4 mmol/kg caused reduced glutathione concentration until experimental day 8. In the 0.067-J/cm2 UV-B irradiation exp...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000267957
更新日期:1996-01-01 00:00:00
abstract:BACKGROUND:The aim of this work was to evaluate the narrow anterior chamber angle (ACA) in Chinese patients by anterior segment optical coherence tomography (As-OCT). METHODS:Eighty right eyes of 80 consecutive patients with a shallow peripheral anterior chamber but no peripheral anterior synechia were enrolled in thi...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000448523
更新日期:2017-01-01 00:00:00
abstract::In a search for a pharmacologic adjuvant in the management of posttraumatic proliferative vitreoretinopathy (PVR), we investigated the effect of intravitreal injection of prinomastat (AG3340) on an experimental model. Posterior penetrating eye trauma was created in one eye each of 24 New Zealand white rabbits. One wee...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000055636
更新日期:2001-01-01 00:00:00
abstract::A system for isolation of capillaries from the retina has been adapted to biochemical studies in vitro. Insulin receptors were identified in human and calf retinal blood vessels. Binding of 125I insulin by calf retinal blood vessels takes place through high affinity (low capacity) and low affinity (high capacity) rece...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265234
更新日期:1983-01-01 00:00:00
abstract:OBJECTIVE:In this study, we used a rat model of retinal detachment (RD) to investigate the effects of transient receptor potential mucolipin 1 (TRPML1) on photoreceptor cells and the underlying mechanism. METHODS:An RD model was established by subretinal injection of sodium hyaluronate, and mucolipin synthetic agonist...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000512104
更新日期:2020-10-07 00:00:00
abstract::Indirect measurement in vivo of nuclear color of 64 cataractous eyes was made using a method of image analysis of photographed lenses developed by the authors. The grade of yellow of lens color was characterized by its dominant wavelength and excitation purity in the CIE chromaticity diagram. The differences were smal...
journal_title:Ophthalmic research
pub_type: 杂志文章
doi:10.1159/000265345
更新日期:1985-01-01 00:00:00