High reproducibility is attainable in assessing histoprognostic parameters of pT1 colorectal cancer using routine histopathology slides and immunohistochemistry analyses.


:Assessment of the risk of lymph node invasion and tumour recurrence is critical to determine whether additional surgery is required in patients with endoscopically-removed pT1 colorectal cancer (CRC). A reproducible assessment of this risk of recurrence based on histopathological parameters is crucial for relevant therapeutic decisions. The inter-observer reproducibility of these parameters was the subject of our study. Two pathologists independently analysed 163 endoscopically-removed pT1 CRC recorded in a local digestive cancer registry database (Finistère, France). Using haematoxylin-eosin-saffron (HES) and immunohistochemistry slides, they evaluated several parameters related to the risk of tumour recurrence according to the international pT1 CRC-dedicated guidelines. Based on Kappa and intra-class correlation coefficients, good to very good inter-observer agreement was obtained by analysing vertical and lateral margins, submucosal invasion, tumour differentiation and lymphovascular invasion. The reproducibility of tumour budding quantification was only fair on the basis of HES slides but reached a very good agreement using cytokeratin immunohistochemistry. Dual colour cytokeratin and podoplanin immunohistochemistry also improved inter-observer agreement for the detection of lymphovascular invasion. All patients with loco-regional nodal metastases (7 of 101 who underwent complementary surgery) or distant metastases (3 patients) were diagnosed as having a high risk of recurrence and requiring an additional surgery by the two observers. Our study showed that good to very good inter-observer agreement is achievable in evaluating the pathological parameters of recurrence risk in endoscopically-removed pT1 CRC. In addition to HES slides, the detection of lymphovascular invasion and tumour budding can benefit with more reproducible immunohistochemical analyses.






Barel F,Auffret A,Cariou M,Kermarrec T,Samaison L,Bourhis A,Badic B,Jézéquel J,Cholet F,Bail JP,Marcorelles P,Nousbaum JB,Robaszkiewicz M,Doucet L,Uguen A




Has Abstract


2019-01-01 00:00:00














  • Aeromonas septicemia: relationship of species and clinical features.

    abstract::Thirteen patients with Aeromonas species septicemia were seen at Westmead Hospital between 1983 and 1987. In 10 patients (77%) septicemia was caused by A. sobria, and in 3 (23%) by A. hydrophila, A. caviae was not isolated. Chronic underlying illness was present in 10 patients (77%), hematological malignancy being the...


    pub_type: 杂志文章


    authors: Dryden M,Munro R

    更新日期:1989-04-01 00:00:00

  • Expression and prognostic value of NSD1 and SETD2 in pancreatic ductal adenocarcinoma and its precursor lesions.

    abstract::Epigenetic regulation is emerging as a critical mechanism for pancreatic ductal adenocarcinoma (PDA) development. Histone methylation is an important regulatory mechanism, altering chromatin structure and promoter accessibility and causing aberrant gene expression. NSD1 and SETD2 genes encoding two histone H3K36 methy...


    pub_type: 杂志文章


    authors: Ettel M,Zhao L,Schechter S,Shi J

    更新日期:2019-06-01 00:00:00

  • Recurrent pyogenic cholangitis: a necropsy study.

    abstract::Forty-six necropsy cases of recurrent pyogenic cholangitis (RPC) among Chinese in Hong Kong were reviewed. Intrahepatic biliary pigment stones or bile sludge were present in all cases and liver abscesses were common (80%). Histologically the early lesion was marked by acute inflammation in portal tracts with frequent ...


    pub_type: 杂志文章


    authors: Chou ST,Chan CW

    更新日期:1980-07-01 00:00:00

  • Chromosome analysis of bone marrow samples from country patients.

    abstract::Co-operation by outside centres has made it possible to offer bone marrow chromosome analysis to patients living up to 1800 kilometres from the central cytogenetics laboratory. Diagnostic karyotypes have been obtained in 9 of 10 bone marrow specimens which were partially processed before being transported to the city ...


    pub_type: 杂志文章


    authors: Bell JA

    更新日期:1978-07-01 00:00:00

  • An ovarian tumour specific mucin antigen--immunohistological and biochemical studies.

    abstract::A new mucin antigen was detected in a mucinous cystadenoma of human ovary. An antiserum produced in rabbits against the crude cyst fluid, following appropriate absorptions, showed immunoreactivity exclusively with tumour epithelium, particularly endocervical type epithelium. The antigen responsible for the immunoreact...


    pub_type: 杂志文章


    authors: Ma J,Handley CJ,de Boer WG

    更新日期:1983-10-01 00:00:00

  • Heparin induced thrombocytopenia. Variable platelet-rich plasma reactivity to heparin dependent platelet aggregating factor.

    abstract::The heparin dependent platelet aggregating factor in the serum from an individual with heparin induced thrombocytopenia exhibited reactivity with only 50% of an extensive panel of normal platelet rich plasmas, and this variability was independent of the platelet rich plasma platelet Factor 4 content. Individual sera o...


    pub_type: 杂志文章


    authors: Salem HH,van der Weyden MB

    更新日期:1983-07-01 00:00:00

  • Detection of copy number variations in melanocytic lesions utilising array based comparative genomic hybridisation.

    abstract::Distinction between melanocytic naevi and melanoma occasionally poses a diagnostic challenge in ambiguous cases showing overlapping histological features. Melanomas are characterised by the presence of multiple genomic copy number variants (CNVs), while this is not a feature of naevi. We assessed the feasibility and u...


    pub_type: 杂志文章


    authors: Mesbah Ardakani N,Thomas C,Robinson C,Mina K,Harvey NT,Amanuel B,Wood BA

    更新日期:2017-04-01 00:00:00

  • Azthreonam: in vitro activity against urinary pathogens.

    abstract::The in vitro activity of azthreonam, a novel monocyclic beta-lactam antibiotic, was investigated for Gram negative urinary pathogens by determining the susceptibilities of 105 consecutive isolates from specimens submitted to the Auckland Hospital microbiology laboratory. All were sensitive or of intermediate sensitivi...


    pub_type: 杂志文章


    authors: Lang SD,McKenzie D

    更新日期:1983-04-01 00:00:00

  • Reduced CD151 expression is related to advanced tumour stage in urothelial bladder cancer.

    abstract:AIMS:CD151 belongs to the group of tetraspanins and is aberrantly expressed in different tumours and differential expression has been associated with prognosis. The aim of this study was to clarify the relationship of CD151 expression with tumour phenotype and clinical outcome in bladder cancer. METHODS:A bladder canc...


    pub_type: 杂志文章


    authors: Minner S,De Silva C,Rink M,Dahlem R,Chun F,Fisch M,Höppner W,Wagner W,Bokemeyer C,Terracciano L,Simon R,Sauter G,Wilczak W

    更新日期:2012-08-01 00:00:00

  • The fetal adrenal gland: definitive cortex cystic change, lipid patterns, and their relationship to fetal disease and maturity.

    abstract::A study of 103 fetal adrenals obtained from stillborn fetuses and those dying in the neonatal period was performed to assess the significance of definitive cortex cystic change, compact cell change and lipid patterns, and their relationship to other pathological processes. The findings confirm that these changes are m...


    pub_type: 杂志文章


    authors: Delprado WJ,Baird PJ

    更新日期:1984-07-01 00:00:00

  • NF-kappaB signalling proteins p50/p105, p52/p100, RelA, and IKKepsilon are over-expressed in oesophageal squamous cell carcinomas.

    abstract:AIMS:Nuclear factor-kappa B (NF-kappaB) activation has been recognised as an important mechanism in the development of cancers. However, expression status of NF-kappaB-related proteins in oesophageal squamous cell carcinoma (ESCC) tissues is largely unknown. In this study, we analysed expressions of NF-kappaB members (...


    pub_type: 杂志文章


    authors: Kang MR,Kim MS,Kim SS,Ahn CH,Yoo NJ,Lee SH

    更新日期:2009-01-01 00:00:00

  • Tumour necrosis factor receptor-associated factor-1 (TRAF-1) expression is increased in renal cell carcinoma patient serum but decreased in cancer tissue compared with normal: potential biomarker significance.

    abstract::Renal cell carcinoma (RCC) generally has a poor prognosis because of late diagnosis and metastasis. We have previously described decreased tumour necrosis factor receptor-associated factor-1 (TRAF-1) in RCC compared with paired normal kidney in a patient cohort in Australia. In the present study, TRAF-1 expression in ...


    pub_type: 杂志文章


    authors: Rajandram R,Yap NY,Pailoor J,Razack AH,Ng KL,Ong TA,Morais C,Gobe GC

    更新日期:2014-10-01 00:00:00

  • von Willebrand's disease characterized by increased ristocetin sensitivity and the presence of all von Willebrand factor multimers in plasma: a new subtype.

    abstract::The clinical and laboratory features of a patient with a recently recognized new variant of von Willebrand's disease are presented. The importance of this variant is that it is associated with a clinically significant bleeding diathesis but with a normal skin bleeding time, PTTK, factor VIIIc and platelet aggregation ...


    pub_type: 杂志文章


    authors: Wylie B,Gibson J,Uhr E,Kronenberg H

    更新日期:1988-01-01 00:00:00

  • Not only 'Flinders Island' spotted fever.

    abstract:AIM:To demonstrate that Flinders Island spotted fever (FISF), a spotted fever group rickettsial infection caused by Rickettsia honei, is found not only on Flinders Island (Bass Strait), Tasmania, but elsewhere in south-east Australia. METHODS:Cases of FISF were identified by rickettsial serology, culture and the detec...


    pub_type: 杂志文章


    authors: Unsworth NB,Stenos J,McGregor AR,Dyer JR,Graves SR

    更新日期:2005-06-01 00:00:00

  • The elaboration of a critical framework for understanding cancer: the cancer stem cell hypothesis.

    abstract::The cancer stem cell hypothesis suggests that malignant tumours may arise from a limited number of specialised cells possessing the key 'stem' properties of self-renewal and the ability to produce differentiated progeny. Such cells purportedly constitute a small fraction of most tumours but have greater potential to p...


    pub_type: 杂志文章,评审


    authors: Hemmings C

    更新日期:2010-02-01 00:00:00

  • Mesocolonic lymphangioma: a case report with immunoperoxidase and electron microscopic studies.

    abstract::A 14 yr old girl presented with a large cystic mass in the transverse mesocolon which was completely excised. Cystic lymphangioma was diagnosed, based on the specific histologic, immunoperoxidase and ultrastructural findings. ...


    pub_type: 杂志文章


    authors: Yuen ST,Ng IO,Ho J,Ma L

    更新日期:1992-07-01 00:00:00

  • Increased caveolin-1 expression associated with prolonged overall survival rate in hepatocellular carcinoma.

    abstract:AIMS:Recent study indicates that the binding of caveolin-1 (CAV1), the essential constituent of caveolae, to endothelial nitric oxide synthase (eNOS) prevents nitric oxide (NO) production in cirrhotic human liver. However, their interplay in hepatocellular carcinoma (HCC) remains undetermined. METHODS:Paraffin-embedde...


    pub_type: 杂志文章


    authors: Yang SF,Yang JY,Huang CH,Wang SN,Lu CP,Tsai CJ,Chai CY,Yeh YT

    更新日期:2010-01-01 00:00:00

  • Teratoid carcinosarcoma of the paranasal sinuses.

    abstract::Malignant tumours with teratoid or blastomatous features are exceptionally rare in the upper respiratory tract with only 8 documented cases, including the 3 in this report. The tumours occurred in adults aged 27 to 62 yr, and the sites of origin were the ethmoid sinus (4 cases), ethmoid and other paranasal sinuses (2 ...


    pub_type: 杂志文章


    authors: Shanmugaratnam K,Kunaratnam N,Chia KB,Chiang GS,Sinniah R

    更新日期:1983-10-01 00:00:00

  • The enigma of struma ovarii.

    abstract::Since its first description in the early part of the twentieth century, struma ovarii has elicited considerable interest because of its many unique features; however, at present a number of aspects remain enigmatic. Although the typical presentation is that of a pelvic mass, unusual clinical manifestations such as hyp...


    pub_type: 杂志文章,评审


    authors: Roth LM,Talerman A

    更新日期:2007-02-01 00:00:00

  • Virological diagnosis of Ebolavirus infection.

    abstract::Ebolaviruses, and the other viral causes of haemorrhagic fevers (VHF) have always posed special problems for diagnostic laboratories. These arise from the rarity of human infections, minimal documented experience with test delivery and interpretation, the paucity of established commercial or in-house assays, the lack ...


    pub_type: 杂志文章,评审


    authors: Smith DW,Rawlinson WD,Kok J,Dwyer DE,Catton M

    更新日期:2015-08-01 00:00:00

  • Acute tonsillitis in children: microbial pathogens in relation to age.

    abstract::Tonsil swabs were collected from 248 children (1 through 10 yr) who presented with acute tonsillitis. A beta-hemolytic streptococcus was grown from 129 (53%) of 244 cases and as the sole pathogen from 83 (47%) of 178 cases. Beta-Hemolytic streptococci were especially common over the age of 6 yr (68% of children) relat...


    pub_type: 杂志文章


    authors: Douglas RM,Miles H,Hansman D,Fadejevs A,Moore B,Bollen MD

    更新日期:1984-01-01 00:00:00

  • Local renal graft-versus-host reaction: sequential ultrastructural study.

    abstract::A sequential study has been made of the ultrastructure of local renal graft-versus-host reactions produced in cyclophosphamide-pretreated rats by injection of either allogeneic or xenogeneic (mouse) spleen cells beneath the renal capsule. Early in the reaction, immunoblasts were seen between the outer cortical tubules...


    pub_type: 杂志文章


    authors: Davies DJ,Conway MS

    更新日期:1975-10-01 00:00:00

  • Recent advances in the diagnosis of soft tissue tumours.

    abstract::Soft tissue tumours are relatively rare, but are diagnostically challenging as they comprise a large spectrum of diagnostic entities. Substantial advances have been made in recent years in identifying the underlying recurrent chromosomal and genomic alterations in a significant subset of soft tissue tumours, and this ...


    pub_type: 杂志文章,评审


    authors: Schaefer IM,Fletcher CDM

    更新日期:2018-01-01 00:00:00

  • The preparation of glomeruli from renal biopsy specimens for scanning electron microscopy.

    abstract::A method for the preparation of human renal biopsy specimens for scanning electron microscopy (SEM) is described. This involves glomerular isolation by fine needle dissection and the simultaneous processing of 10 or more specimens in a specially designed tissue cassette. The method is simple and reliable with minimal ...


    pub_type: 杂志文章


    authors: Ng W,So KF,So PC,Ngai HK

    更新日期:1982-07-01 00:00:00

  • The effect of 50 grams of glucose orally on a number of biochemical variables.

    abstract::The values of a number of biochemical variables have been studied before and after a 50-gram load of glucose orally. Reductions which were statistically significant were found for sodium, potassium, urea, total protein, albumin, calcium, phosphorus, urate, bilirubin, alkaline phosphatase, but not for bicarbonate, crea...


    pub_type: 杂志文章


    authors: Masarei JR

    更新日期:1977-01-01 00:00:00

  • 'Why don't they ever call?' Expectations of clinicians and pathologists regarding the communication of critical diagnoses in dermatopathology.

    abstract::Certain diagnoses in dermatopathology have significant implications for patient management and on occasion appropriate clinical care may be facilitated by a phone call from the reporting dermatopathologist to the referring doctor. Whether this is appropriate depends on a number of factors. The concept of 'critical dia...


    pub_type: 杂志文章


    authors: Korbl JD,Wood BA,Harvey NT

    更新日期:2018-04-01 00:00:00

  • Histopathology of the stapes in otosclerosis.

    abstract::A histopathological examination was made of 60 stapes obtained during surgery for clinical otosclerosis. The findings, correlated with the clinical history and macroscopic appearances of the stapes, led to the following observations. The histological extent of the focus of otosclerosis agrees well with the macroscopic...


    pub_type: 杂志文章


    authors: Iyer PV,Gristwood RE

    更新日期:1984-01-01 00:00:00

  • Lobular in situ neoplasia and columnar cell lesions: diagnosis in breast core biopsies and implications for management.

    abstract::Histopathologists are encountering intra-lobular epithelial proliferations more frequently in core biopsies taken from lesions identified in mammographic breast screening programmes. In particular, columnar cell lesions are increasingly being seen in core biopsies taken for the histological assessment of mammographica...


    pub_type: 杂志文章,评审


    authors: Pinder SE,Provenzano E,Reis-Filho JS

    更新日期:2007-04-01 00:00:00

  • Determination of the optimum incubation period of continuously monitored blood cultures from patients with suspected endocarditis or fungaemia.

    abstract:AIM:It is routine practice to prolong the incubation period of blood cultures from patients with suspected subacute bacterial endocarditis (SBE) or fungal infection. The protocol in this laboratory required 28 days incubation with weekly subcultures. Following the introduction of automated continuously monitored blood ...


    pub_type: 杂志文章


    authors: Varettas K,Taylor PC,Mukerjee C

    更新日期:2002-04-01 00:00:00

  • Hepatic granuloma in a man from North America caused by a nymph of Linguatula serrata.

    abstract::A calcified nodule on the liver of a 62-year-old man from North Carolina, USA, contained a degenerated nymph of Linguatula serrata. The nodule was incidentally discovered at laparotomy for malignant lymphoma and cholelithiasis. The cuticle was all that remained of the parasite, but sclerotized openings and large spine...


    pub_type: 杂志文章


    authors: Baird JK,Kassebaum LJ,Ludwig GK

    更新日期:1988-04-01 00:00:00