Abstract：
Background:Toxoplasmosis is a common infection all around the world. During pregnancy; it may lead to congenital disorders or abortion in human and animals. Severe damage of toxoplasmosis indicates to require effective vaccine. One of dense granules antigen is GRA4 that secrete from tachyzoite and bradyzoite. GRA4 genome is unique without intron and is one of the major immunogenic proteins from Toxoplasma gondii. Methods:We confirmed the cloning of GRA4 gene into pcDNA3 by restriction enzyme and PCR of GRA4 gene with pcGRA4 plasmids as template. Then with using calcium-phosphate method we transfected the pcGRA4 into CHO (Chinesehamster ovary) cells. The yielded protein was separated by SDS-PAGE and moved by electroblotting to nitrocellulose paper. Results:Result of SDS-PAGE analysis showed the appearance of band approximately 42 kDa which was absent in the negative control, that was able to identify toxoplasmosis antibody IgM+ serum in western blot analysis. Conclusion:pcGRA4 plasmid is able to synthesis of antigenic protein in CHO cells. The ability of pcGRA4 for induction of protective immune response against toxoplasmosis will be evaluated in mouse model.
journal_name
Iran J Parasitoljournal_title
Iranian journal of parasitologyauthors
Aghdasi M,Ghaffarifar F,Forooghi F,Dalimi Asl AH,Sharifi Z,Maspi Nsubject
Has Abstractpub_date
2018-07-01 00:00:00pages
392-398issue
3eissn
1735-7020issn
2008-238Xjournal_volume
13pub_type
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journal_title：Iranian journal of parasitology
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journal_title：Iranian journal of parasitology
pub_type： 杂志文章
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journal_title：Iranian journal of parasitology
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