Comparative Transcriptomic and Metagenomic Analyses of Influenza Virus-Infected Nasal Epithelial Cells From Multiple Individuals Reveal Specific Nasal-Initiated Signatures.


:In vitro and in vivo research based on cell lines and animals are likely to be insufficient in elucidating authentic biological and physiological phenomena mimicking human systems, especially for generating pre-clinical data on targets and biomarkers. There is an obvious need for a model that can further bridge the gap in translating pre-clinical findings into clinical applications. We have previously generated a model of in vitro differentiated human nasal epithelial cells (hNECs) which elucidated the nasal-initiated repertoire of immune responses against respiratory viruses such as influenza A virus and rhinovirus. To assess their clinical utility, we performed a microarray analysis of influenza virus-infected hNECs to elucidate nasal epithelial-initiated responses. This was followed by a metagenomic analysis which revealed transcriptomic changes comparable with clinical influenza datasets. The primary target of influenza infection was observed to be the initiator of innate and adaptive immune genes, leaning toward type-1 inflammatory activation. In addition, the model also elucidated a down-regulation of metabolic processes specific to the nasal epithelium, and not present in other models. Furthermore, the hNEC model detected all 11 gene signatures unique to influenza infection identified from a previous study, thus supporting the utility of nasal-based diagnosis in clinical settings. In conclusion, this study highlights that hNECs can serve as a model for nasal-based clinical translational studies and diagnosis to unravel nasal epithelial responses to influenza in the population, and as a means to identify novel molecular diagnostic markers of severity.


Front Microbiol


Tan KS,Yan Y,Koh WLH,Li L,Choi H,Tran T,Sugrue R,Wang Y,Chow VT




Has Abstract


2018-11-14 00:00:00








  • Gene Turnover Contributes to the Evolutionary Adaptation of Acidithiobacillus caldus: Insights from Comparative Genomics.

    abstract::Acidithiobacillus caldus is an extremely acidophilic sulfur-oxidizer with specialized characteristics, such as tolerance to low pH and heavy metal resistance. To gain novel insights into its genetic complexity, we chosen six A. caldus strains for comparative survey. All strains analyzed in this study differ in geograp...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Zhang X,Liu X,He Q,Dong W,Zhang X,Fan F,Peng D,Huang W,Yin H

    更新日期:2016-12-06 00:00:00

  • Gene polymorphisms in pattern recognition receptors and susceptibility to idiopathic recurrent vulvovaginal candidiasis.

    abstract:OBJECTIVE:Approximately 5% of women suffer from recurrent vulvovaginal candidiasis (RVVC). It has been hypothesized that genetic factors play an important role in the susceptibility to RVVC. The aim of this study was to assess the effect of genetic variants of genes encoding for pattern recognition receptors (PRRs) on ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Rosentul DC,Delsing CE,Jaeger M,Plantinga TS,Oosting M,Costantini I,Venselaar H,Joosten LA,van der Meer JW,Dupont B,Kullberg BJ,Sobel JD,Netea MG

    更新日期:2014-09-23 00:00:00

  • Responses of beneficial Bacillus amyloliquefaciens SQR9 to different soilborne fungal pathogens through the alteration of antifungal compounds production.

    abstract::Bacillus amyloliquefaciens SQR9 exhibited predominantly antagonistic activities against a broad range of soilborne pathogens. The fungi-induced SQR9 extracts possess stronger antifungal activities compared with SQR9 monoculture extracts. To investigate how SQR9 fine-tunes lipopeptides (LPs) and a siderophore bacilliba...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Li B,Li Q,Xu Z,Zhang N,Shen Q,Zhang R

    更新日期:2014-11-21 00:00:00

  • Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections.

    abstract::Phage therapy requires libraries of well-characterized phages. Here we describe the generation of phage libraries for three target species: Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. The basic phage characteristics on the isolation host, sequence analysis, growth properties, and host range and...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Gibson SB,Green SI,Liu CG,Salazar KC,Clark JR,Terwilliger AL,Kaplan HB,Maresso AW,Trautner BW,Ramig RF

    更新日期:2019-11-12 00:00:00

  • The Diverse Search for Synthetic, Semisynthetic and Natural Product Antibiotics From the 1940s and Up to 1960 Exemplified by a Small Pharmaceutical Player.

    abstract::The 1940s and 1950s witnessed a diverse search for not just natural product antibiotics but also for synthetic and semisynthetic compounds. This review revisits this epoch, using the research by a Danish pharmaceutical company, LEO Pharma, as an example. LEO adopted a strategy searching for synthetic antibiotics towar...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Leisner JJ

    更新日期:2020-06-12 00:00:00

  • Genomic analysis of six new Geobacillus strains reveals highly conserved carbohydrate degradation architectures and strategies.

    abstract::In this work we report the whole genome sequences of six new Geobacillus xylanolytic strains along with the genomic analysis of their capability to degrade carbohydrates. The six sequenced Geobacillus strains described here have a range of GC contents from 43.9% to 52.5% and clade with named Geobacillus species throug...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Brumm PJ,De Maayer P,Mead DA,Cowan DA

    更新日期:2015-05-12 00:00:00

  • Genetic Identification of a PilT Motor in Geobacter sulfurreducens Reveals a Role for Pilus Retraction in Extracellular Electron Transfer.

    abstract::The metal-reducing bacterium Geobacter sulfurreducens requires the expression of conductive pili to reduce iron oxides and to wire electroactive biofilms, but the role of pilus retraction in these functions has remained elusive. Here we show that of the four PilT proteins encoded in the genome of G. sulfurreducens, Pi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Speers AM,Schindler BD,Hwang J,Genc A,Reguera G

    更新日期:2016-10-17 00:00:00

  • A Candidate Secreted Effector Protein of Rubber Tree Powdery Mildew Fungus Contributes to Infection by Regulating Plant ABA Biosynthesis.

    abstract::Powdery mildew infects a wide range of crops and economic plants, causing substantial losses. Rubber trees (Hevea brasiliensis) are the primary source of natural rubber, and powdery mildew infection causes significant losses to natural rubber yields. How the causal agent, Erysiphe quercicola, establishes successful in...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Li X,Liu Y,He Q,Li S,Liu W,Lin C,Miao W

    更新日期:2020-11-24 00:00:00

  • Prevalence and Antibiotic Resistance of Non-typhoidal Salmonella Isolated from Raw Chicken Carcasses of Commercial Broilers and Spent Hens in Tai'an, China.

    abstract::The present study was aimed to determine the prevalence and characteristics of Salmonella isolated from meat samples of commercial broilers (CB) and spent hens (SH). Between March and June 2016, 200 retail raw chicken carcasses (100 from CB and 100 from SH) were obtained from local supermarkets in Tai'an city of China...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Li S,Zhou Y,Miao Z

    更新日期:2017-10-31 00:00:00

  • A modular method for the extraction of DNA and RNA, and the separation of DNA pools from diverse environmental sample types.

    abstract::A method for the extraction of nucleic acids from a wide range of environmental samples was developed. This method consists of several modules, which can be individually modified to maximize yields in extractions of DNA and RNA or separations of DNA pools. Modules were designed based on elaborate tests, in which permu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Lever MA,Torti A,Eickenbusch P,Michaud AB,Šantl-Temkiv T,Jørgensen BB

    更新日期:2015-05-19 00:00:00

  • Competition Experiments for Legume Infection Identify Burkholderia phymatum as a Highly Competitive β-Rhizobium.

    abstract::Members of the genus Burkholderia (β-proteobacteria) have only recently been shown to be able to establish a nitrogen-fixing symbiosis with several legumes, which is why they are also referred to as β-rhizobia. Therefore, very little is known about the competitiveness of these species to nodulate different legume host...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Lardi M,de Campos SB,Purtschert G,Eberl L,Pessi G

    更新日期:2017-08-15 00:00:00

  • SMRT Sequencing Revealed Mitogenome Characteristics and Mitogenome-Wide DNA Modification Pattern in Ophiocordyceps sinensis.

    abstract::Single molecule, real-time (SMRT) sequencing was used to characterize mitochondrial (mt) genome of Ophiocordyceps sinensis and to analyze the mt genome-wide pattern of epigenetic DNA modification. The complete mt genome of O. sinensis, with a size of 157,539 bp, is the fourth largest Ascomycota mt genome sequenced to ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Kang X,Hu L,Shen P,Li R,Liu D

    更新日期:2017-07-27 00:00:00

  • Transcriptome Analysis Reveals the Flexibility of Cordycepin Network in Cordyceps militaris Activated by L-Alanine Addition.

    abstract::Cordycepin, isolated from the traditional medicinal fungus Cordyceps militaris, has gained much attention due to its various clinical functions. Previous reports of L-alanine addition could significantly improve cordycepin production, but the molecular mechanism remains unknown. In this study, transcriptome analysis o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Chen BX,Wei T,Xue LN,Zheng QW,Ye ZW,Zou Y,Yang Y,Yun F,Guo LQ,Lin JF

    更新日期:2020-04-24 00:00:00

  • Modeling the Inactivation of Intestinal Pathogenic Escherichia coli O157:H7 and Uropathogenic E. coli in Ground Chicken by High Pressure Processing and Thymol.

    abstract::Disease causing Escherichia coli commonly found in meat and poultry include intestinal pathogenic E. coli (iPEC) as well as extraintestinal types such as the Uropathogenic E. coli (UPEC). In this study we compared the resistance of iPEC (O157:H7) to UPEC in chicken meat using High Pressure Processing (HPP) in with (th...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Chien SY,Sheen S,Sommers CH,Sheen LY

    更新日期:2016-06-14 00:00:00

  • In vitro and in vivo Virulence Potential of the Emergent Species of the Acinetobacter baumannii (Ab) Group.

    abstract::The increased use of molecular identification methods and mass spectrometry has revealed that Acinetobacter spp. of the A. baumannii (Ab) group other than A. baumannii are increasingly being recovered from human samples and may pose a health challenge if neglected. In this study 76 isolates of 5 species within the Ab ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Cosgaya C,Ratia C,Marí-Almirall M,Rubio L,Higgins PG,Seifert H,Roca I,Vila J

    更新日期:2019-10-24 00:00:00

  • The Road to Infection: Host-Microbe Interactions Defining the Pathogenicity of Streptococcus bovis/Streptococcus equinus Complex Members.

    abstract::The Streptococcus bovis/Streptococcus equinus complex (SBSEC) comprises several species inhabiting the animal and human gastrointestinal tract (GIT). They match the pathobiont description, are potential zoonotic agents and technological organisms in fermented foods. SBSEC members are associated with multiple diseases ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Jans C,Boleij A

    更新日期:2018-04-10 00:00:00

  • In situ Immune Signatures and Microbial Load at the Nasopharyngeal Interface in Children With Acute Respiratory Infection.

    abstract::Acute respiratory infection (ARI) is the most frequent cause for hospitalization in infants and young children. Using multiplexed nCounter technology to digitally quantify 600 human mRNAs in parallel with 14 virus- and 5 bacterium-specific RNAs, we characterized viral and bacterial presence in nasopharyngeal aspirates...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Fukutani KF,Nascimento-Carvalho CM,Bouzas ML,Oliveira JR,Barral A,Dierckx T,Khouri R,Nakaya HI,Andrade BB,Van Weyenbergh J,de Oliveira CI

    更新日期:2018-11-09 00:00:00

  • To Construct an Engineered (S)-Equol Resistant E. coli for in Vitro (S)-Equol Production.

    abstract::(S)-equol is one of the major metabolites of daidzein that is produced by human and animal gut bacteria. Most of the physiological functions of soybean isoflavones, such as anti-oxidative activity, anti-cancer activity, and cardiovascular protection have been ascribed to (S)-equol. However, only 30-50% people contain ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Li H,Mao S,Chen H,Zhu L,Liu W,Wang X,Yin Y

    更新日期:2018-06-04 00:00:00

  • Determining the Distribution of Fluorescent Organic Matter in the Indian Ocean Using in situ Fluorometry.

    abstract::In order to determine the dynamics of marine fluorescent organic matter (FOM) using high-resolution spatial data, in situ fluorometers have been used in the open ocean. In this study, we measured FOM during the Global Ocean Ship-based Hydrographic Investigations Program (GO-SHIP) expedition from early December 2019 to...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Shigemitsu M,Uchida H,Yokokawa T,Arulananthan K,Murata A

    更新日期:2020-12-23 00:00:00

  • Fungal Enolase, β-Tubulin, and Chitin Are Detected in Brain Tissue from Alzheimer's Disease Patients.

    abstract::Recent findings provide evidence that fungal structures can be detected in brain tissue from Alzheimer's disease (AD) patients using rabbit polyclonal antibodies raised against whole fungal cells. In the present work, we have developed and tested specific antibodies that recognize the fungal proteins, enolase and β-tu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Pisa D,Alonso R,Rábano A,Horst MN,Carrasco L

    更新日期:2016-11-07 00:00:00

  • Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry-Based Metabolomics Reveals Metabolic Alterations in the Mouse Cerebellum During Toxoplasma gondii Infection.

    abstract::Toxoplasma gondii is a protozoan parasite with a remarkable neurotropism. We recently showed that T. gondii infection can alter the global metabolism of the cerebral cortex of mice. However, the impact of T. gondii infection on the metabolism of the cerebellum remains unknown. Here we apply metabolomic profiling to di...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Ma J,He JJ,Hou JL,Zhou CX,Elsheikha HM,Zhu XQ

    更新日期:2020-07-10 00:00:00

  • The Nuclear-Localized RxLR Effector PvAvh74 From Plasmopara viticola Induces Cell Death and Immunity Responses in Nicotiana benthamiana.

    abstract::Downy mildew is one of the most serious diseases of grapevine (Vitis spp). The causal agent of grapevine downy mildew, Plasmopara viticola, is an obligate biotrophic oomycete. Although oomycete pathogens such as P. viticola are known to secrete RxLR effectors to manipulate host immunity, there have been few studies of...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Yin X,Shang B,Dou M,Liu R,Chen T,Xiang G,Li Y,Liu G,Xu Y

    更新日期:2019-07-10 00:00:00

  • Characterization of an Omega-3 Desaturase From Phytophthora parasitica and Application for Eicosapentaenoic Acid Production in Mortierella alpina.

    abstract::Omega-3 long-chain polyunsaturated fatty acids (LC-PUFAs) have important therapeutic and nutritional benefits in humans. In the biosynthesis pathways of these LC-PUFAs, omega-3 desaturase plays a critical role. In this study, we report a new omega-3 desaturase (PPD17) from Phytophthora parasitica. This desaturase shar...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Tang X,Chen H,Mei T,Ge C,Gu Z,Zhang H,Chen YQ,Chen W

    更新日期:2018-08-14 00:00:00

  • Intestinal Short Chain Fatty Acids and their Link with Diet and Human Health.

    abstract::The colon is inhabited by a dense population of microorganisms, the so-called "gut microbiota," able to ferment carbohydrates and proteins that escape absorption in the small intestine during digestion. This microbiota produces a wide range of metabolites, including short chain fatty acids (SCFA). These compounds are ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Ríos-Covián D,Ruas-Madiedo P,Margolles A,Gueimonde M,de Los Reyes-Gavilán CG,Salazar N

    更新日期:2016-02-17 00:00:00

  • Endophytic Bacterial Community Structure and Function of Herbaceous Plants From Petroleum Hydrocarbon Contaminated and Non-contaminated Sites.

    abstract::Bacterial endophytes (BEs) are non-pathogenic residents of healthy plant tissues that can confer benefits to plants. Many Bacterial endophytes have been shown to contribute to plant growth and health, alleviation of plant stress and to in-planta contaminant-degradation. This study examined the endophytic bacterial com...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Lumactud R,Fulthorpe RR

    更新日期:2018-08-23 00:00:00

  • Diet, Microbiota and Gut-Lung Connection.

    abstract::The gut microbial community (Gut microbiota) is known to impact metabolic functions as well as immune responses in our body. Diet plays an important role in determining the composition of the gut microbiota. Gut microbes help in assimilating dietary nutrients which are indigestible by humans. The metabolites produced ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Anand S,Mande SS

    更新日期:2018-09-19 00:00:00

  • Non-pylori Helicobacters (NHPHs) Induce Shifts in Gastric Microbiota in Helicobacter pylori-Infected Patients.

    abstract::To explore the effects of gastric non-H. pylori Helicobacter species(NHPH) on the structure and potential function of gastric microbiota, we employed 16S rRNA gene sequencing on 164 gastric biopsy specimens from NHPH (H. suis, H. felis, H. salomonis) /H. pylori coinfection individuals, H. pylori monoinfection individu...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Peng X,Zhou L,Gong Y,Song Z,He L,Lin S,Zhang J

    更新日期:2017-06-08 00:00:00

  • Phosphoproteomics analysis of a clinical Mycobacterium tuberculosis Beijing isolate: expanding the mycobacterial phosphoproteome catalog.

    abstract::Reversible protein phosphorylation, regulated by protein kinases and phosphatases, mediates a switch between protein activity and cellular pathways that contribute to a large number of cellular processes. The Mycobacterium tuberculosis genome encodes 11 Serine/Threonine kinases (STPKs) which show close homology to euk...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Fortuin S,Tomazella GG,Nagaraj N,Sampson SL,Gey van Pittius NC,Soares NC,Wiker HG,de Souza GA,Warren RM

    更新日期:2015-02-10 00:00:00

  • The Importance of H in Particulate Organic Matter Stoichiometry, Export and Energy Flow.

    abstract::The discipline of marine ecological stoichiometry has progressed rapidly over the past two decades, and continues to be at the forefront of microbial oceanography. Most of this effort has been focused on the elements carbon (C) and nitrogen (N), and to a lesser extent phosphorus (P), with little consideration of hydro...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Karl DM,Grabowski E

    更新日期:2017-05-09 00:00:00

  • Antimicrobial active herbal compounds against Acinetobacter baumannii and other pathogens.

    abstract::Bacterial pathogens cause a number of lethal diseases. Opportunistic bacterial pathogens grouped into ESKAPE pathogens that are linked to the high degree of morbidity, mortality and increased costs as described by Infectious Disease Society of America. Acinetobacter baumannii is one of the ESKAPE pathogens which cause...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Tiwari V,Roy R,Tiwari M

    更新日期:2015-06-18 00:00:00