Abstract：
:PEGylation is a biochemical modification process of bioactive molecules with polyethylene glycol (PEG), which lends several desirable properties to proteins/peptides, antibodies, and vesicles considered to be used for therapy or genetic modification of cells. However, PEGylation of proteins is a complex process and can be carried out using more than one strategy that depends on the nature of the protein and the desired application. Proteins of interest are covalently conjugated or non-covalently complexed with inert PEG strings. Purification of PEGylated protein is another critical step, which is mainly carried out based on electrostatic interactions or molecular sizes using chromatography. Several PEGylated drugs are being used for diseases like anemia, kidney disease, multiple sclerosis, hemophilia and cancers. With the advancement and increased specificity of the PEGylation process, the world of drug therapy, and specifically cancer therapy could benefit by utilizing this technique to create more stable and non-immunogenic therapies. In this article we describe the structure and functions of PEGylation and how this chemistry helps in drug discovery. Moreover, special emphasis has been given to CCN-family proteins that can be targeted or used as therapy to prevent or block cancer progression through PEGylation technology.
journal_name
J Cell Commun Signaljournal_title
Journal of cell communication and signalingauthors
Gupta V,Bhavanasi S,Quadir M,Singh K,Ghosh G,Vasamreddy K,Ghosh A,Siahaan TJ,Banerjee S,Banerjee SKdoi
10.1007/s12079-018-0492-0subject
Has Abstractpub_date
2019-09-01 00:00:00pages
319-330issue
3eissn
1873-9601issn
1873-961Xpii
10.1007/s12079-018-0492-0journal_volume
13pub_type
杂志文章,评审abstract：:Mice lacking the pro-adhesive matricellular protein connective tissue growth factor (CTGF/CCN2) display an embryonic lethal phenotype due to defects in bone and cartilage. However, the specific role of CCN2 in skin development is unknown. Here, we generated mice deleted for CCN2 in the entire body (using a cre/lox sys...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-011-0129-z
更新日期：2011-08-01 00:00:00
abstract：:In breast cancer cells, the linoleic acid (LA), an ω-6 essential polyunsaturated fatty acid, induces a variety of biological processes, including migration and invasion. Extracellular vesicles (EVs) are structures released by normal and malignant cells into extracellular space, and their function is dependent on their...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-018-0490-2
更新日期：2019-06-01 00:00:00
abstract：:CCN3 is a member of the CCN family of cell growth and differentiation regulators that play key roles during embryonic development, and are associated with severe human pathologies. The level of CCN genes' expression is of prognostic value in several types of tumors. In the present manuscript, we report the isolation a...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-007-0009-8
更新日期：2007-09-01 00:00:00
abstract：:Glutamine (gln) metabolism has emerged as a cancer therapeutic target in past few years, however, the effect of gln-deprivation of bCSCs remains elusive in breast cancer. In this study, effect of glutamine on stemness and differentiation potential of bCSCs isolated from MCF-7 and MDAMB-231 were studied. We have shown ...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-020-00603-1
更新日期：2021-01-28 00:00:00
abstract：:The Fifth International Workshop on the CCN Family of Genes was held in Toronto October 18-22, 2008. This bi-annual workshop provides a unique opportunity for the presentation and discussion of cutting edge research in the CCN field. The CCN family members have emerged as extracellular matrix associated proteins which...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-009-0036-8
更新日期：2008-12-01 00:00:00
abstract：:In June 2015, Thomson Reuters informed our publisher Springer that the Journal of Cell Communication and Signaling, the official journal of the International CCN Society, « had been selected for coverage in Thomson Reuter's products and services. Beginning with V. 1 (1) 2007, this publication would be indexed and abst...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-015-0306-6
更新日期：2015-09-01 00:00:00
abstract：:Tumor cells release extracellular vesicles (EVs) into the tumor microenvironment that may facilitate malignant progression and metastasis. Breast carcinoma EVs express high levels of the thrombospondin-1 and signal regulatory protein-α receptor CD47, which is the target of several experimental therapeutics currently i...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0428-0
更新日期：2018-03-01 00:00:00
abstract：:Inflammation is a crucial component in the pathogenesis of many vascular diseases, such as atherosclerosis and diabetes. Inflammasomes are intracellular signalling complexes whose activation promotes inflammation. Nucleotide-binding domain and Leucine-rich repeat Receptor containing a Pyrin domain 3 (NLRP3) is a patte...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0396-4
更新日期：2017-09-01 00:00:00
abstract：:Glutamate metabolism plays a vital role in biosynthesis of nucleic acids and proteins. It is also associated with a number of different stress responses. Deficiency of enzymes involved in glutamate metabolism is associated with various disorders including gyrate atrophy, hyperammonemia, hemolytic anemia, γ-hydoxybutyr...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-015-0315-5
更新日期：2016-03-01 00:00:00
abstract：:Human bronchial epithelial cells exposed to synthetic double-stranded RNA (poly I:C) exhibited increased IL-6 and RANTES secretion and TLR2 expression that was inhibited following TLR3 silencing. Increased NF-κB and Stat3 phosphorylation were detected after poly I:C exposure and pretreatment with neutralizing antibody...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-012-0185-z
更新日期：2013-06-01 00:00:00
abstract：:Hepatocyte exosomes (ExoHep) are proposed to mediate physiological or pathophysiological signaling in a variety of hepatic target cells. ExoHep were purified from the medium of primary mouse hepatocytes or AML12 cells and characterized as ~100 nm nanovesicles that were positive for proteins commonly found in exosomes ...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0421-7
更新日期：2018-03-01 00:00:00
abstract：:Cell adhesion and migration are complex processes that require integrin activation, the formation and dissolution of focal adhesion (FAs), and linkage of actin cytoskeleton to the FAs. The IPP (ILK, PINCH, Parvin) complex regulates FA formation via binding of the adaptor protein ILK to β1 integrin, PINCH and parvin. T...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-013-0207-5
更新日期：2013-12-01 00:00:00
abstract：:Interferon gamma (IFN-ɣ) is a pleiotropic cytokine which plays dual contrasting roles in cancer. Although IFN-ɣ has been clinically used to treat various malignancies, it was recently shown to have protumorigenic activities. Reactive oxygen species (ROS) are overproduced in cancer cells, mainly due to NADPH oxidase ac...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-016-0362-6
更新日期：2017-03-01 00:00:00
abstract：:CCN proteins play crucial roles in development, angiogenesis, cell motility, matrix turnover, proliferation, and other fundamental cell processes. Early embryonic lethality in CCN5 knockout and over-expressing mice led us to characterize CCN5 distribution in early development. Previous papers in this series showed tha...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-012-0176-0
更新日期：2012-12-01 00:00:00
abstract：:There is no therapy for chronic fibroproliferative diseases, in spite of the fact that current health statistics suggest that these (which include cardiovascular disease, pulmonary fibrosis, diabetic nephropathy, liver cirrhosis and systemic sclerosis) have been estimated to cause approximately 45% of the deaths in th...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-011-0156-9
更新日期：2012-03-01 00:00:00
abstract：:We recently reported that transgenic mice with cardiac-restricted overexpression of CCN2/CTGF have substantially increased tolerance towards ischemia/reperfusion injury. The purpose of this study was to investigate to what extent fully differentiated cardiac myocytes are direct targets of CCN2, and to resolve the sign...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-012-0183-1
更新日期：2013-03-01 00:00:00
abstract：:Connexin43 (Cx43) gap junctions expressed in astrocytes can significantly impact neuronal survival in stroke. However, little is known regarding Cx43 spatial and temporal expression during the initial stages of brain ischemia. Using immunohistochemistry and Western blot analysis, we examined Cx43 spatial and temporal ...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0430-6
更新日期：2018-03-01 00:00:00
abstract：:Pancreatitis is an inflammatory condition of the pancreas which, in its chronic form, involves tissue destruction, exocrine and endocrine insufficiency, increased risk of pancreatic cancer, and an extensive fibrotic pathology which is due to unrelenting collagen deposition by pancreatic stellate cells (PSC). In respon...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-014-0220-3
更新日期：2014-06-01 00:00:00
abstract：:The activity of the Insulin-like Growth Factors (IGFs) ligands elicited via their receptors and transduced by various intracellular signal pathways is modulated by the IGF Binding Proteins (IGFBPs). Among all the IGFBPs, IGFBP-2 has been implicated in the regulation of IGF activity in most tissue and organs. Besides b...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-015-0261-2
更新日期：2015-06-01 00:00:00
abstract：:The discovery of the gap junction structure, its functions and the family of the "connexin" genes, has been basically ignored by the major biological disciplines. These connexin genes code for proteins that organize to form membrane-associated hemi-channels, "connexons", co-join with the connexons of neighboring cells...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-010-0108-9
更新日期：2011-03-01 00:00:00
abstract：:Fibrotic diseases are a significant cause of mortality. It is being increasingly appreciated that the cellular microenvironment plays a key role in promoting pathological fibrosis. A previous Bits and Bytes described an elegant series of experiments published by Bruce Riser and colleagues (Am J Pathol. 2009: 174:1725-...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-015-0281-y
更新日期：2015-03-01 00:00:00
abstract：:Lung immaturity is the major cause of morbidity and mortality in premature infants, especially those born <28 weeks of gestation. These infants are at high risk of developing respiratory distress syndrome (RDS), a lung disease caused by insufficient surfactant production and immaturity of saccular/alveolar type II epi...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-017-0443-1
更新日期：2018-03-01 00:00:00
abstract：:The lectin from Canavalia ensiformis (Concanavalin-A, ConA), one of the most abundant lectins known, enables one to mimic biological lectin/carbohydrate interactions that regulate extracellular matrix protein recognition. As such, ConA is known to induce membrane type-1 matrix metalloproteinase (MT1-MMP) which express...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-009-0084-0
更新日期：2010-03-01 00:00:00
abstract：:Extracellular molecules coordinate the multiple signaling pathways spatiotemporally to exchange information between cells during development. Understanding the regulation of these signal molecule-dependent pathways elucidates the mechanism of intercellular crosstalks. CCN2/CTGF is one of the CCN family members that bi...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-018-0487-x
更新日期：2019-03-01 00:00:00
abstract：:The ubiquitin proteasome pathway plays a critical role in regulating many processes in the cell which are important for tumour cell growth and survival. Inhibition of proteasome function has emerged as a powerful strategy for anti-cancer therapy. Clinical validation of the proteasome as a therapeutic target was achiev...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-011-0121-7
更新日期：2011-06-01 00:00:00
abstract：:It is well-established that the expression of CCN family of matricellular proteins is altered in essentially all cancers and, hence, targeting these proteins may be a novel therapeutic approach to treating these diseases. For example, CCN6 (WISP3) is downregulated in aggressive breast cancers, and this phenomenon appe...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-012-0189-8
更新日期：2013-06-01 00:00:00
abstract：:Hepatoblastoma (HB) is one of the most common liver malignancies in children, while the molecular basis of the disease is largely unknown. Therefore, this study aims to explore the key genes and molecular mechanisms of the pathogenesis of HB using a bioinformatics approach. The gene expression dataset GSE131329 was us...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-020-00584-1
更新日期：2020-10-13 00:00:00
abstract：:Transforming growth factor beta (TGF-beta) and connective tissue growth factor (CTGF) have been described to induce the production of extracellular matrix (ECM) proteins and have been reported to be increased in different fibrotic disorders. Skeletal muscle fibrosis is a common feature of Duchenne muscular dystrophy (...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-008-0018-2
更新日期：2007-12-01 00:00:00
abstract：:The aim of this study was to investigate the effects of homocysteine (Hcy), a risk factor for cardiovascular diseases, hypertension, stroke and obesity, on expression of CD36 that regulates uptake of oxidized low-density lipoprotein (Ox-LDL) by adipocytes and differentiation of 3T3-L1 cells to adipocytes. Cell viabili...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章
doi：10.1007/s12079-015-0316-4
更新日期：2016-03-01 00:00:00
abstract：:Periostin is a secretory protein with a multi-domain structure, comprising an amino-terminal cysteine-rich EMI domain, four internal FAS 1 domains, and a carboxyl-terminal hydrophilic domain. These adjacent domains bind to extracellular matrix proteins (type I collagen, fibronectin, tenascin-C, and laminin γ2), and BM...
journal_title：Journal of cell communication and signaling
pub_type： 杂志文章,评审
doi：10.1007/s12079-017-0422-6
更新日期：2018-03-01 00:00:00
