Oncolytic adenovirus Delta-24-RGD induces a widespread glioma proteotype remodeling during autophagy.

Abstract:

:Adenovirus Delta-24-RGD has shown a remarkable efficacy in a phase I clinical trial for glioblastoma. Delta-24-RGD induces autophagy in glioma cells, however, the molecular derangements associated with Delta-24-RGD infection remains poorly understood. Here, proteomics was applied to characterize the glioma metabolic disturbances soon after Delta-24-RGD internalization and late in infection. Minutes post-infection, a rapid survival reprogramming of glioma cells was evidenced by an early c-Jun activation and a time-dependent dephosphorylation of multiple survival kinases. At 48 h post-infection (hpi), a severe intracellular proteostasis impairment was characterized, detecting differentially expressed proteins related to mRNA splicing, cytoskeletal organization, oxidative response, and inflammation. Specific kinase-regulated protein interactomes for Delta-24-RGD-modulated proteome revealed interferences with the activation dynamics of protein kinases C and A (PKC, PKA), tyrosine-protein kinase Src (c-Src), glycogen synthase kinase-3 (GSK-3) as well as serine/threonine-protein phosphatases 1 and 2A (PP1, PP2A) at 48hpi, in parallel with adenoviral protein overproduction. Moreover, the late activation of the nuclear factor kappa B (NF-κB) correlates with the extracellular increment of specific cytokines involved in migration, and activation of different inflammatory cells. Taken together, our integrative analysis provides further insights into the effects triggered by Delta-24-RGD in the modulation of tumor suppression and immune response against glioma. SIGNIFICANCE: The current study provides new insights regarding the molecular mechanisms governing the glioma metabolism during Delta-24-RGD oncolytic adenoviral therapy. The compilation and analysis of intracellular and extracellular proteomics have led us to characterize: i) the cell survival reprogramming during Delta-24-RGD internalization, ii) the proteostatic disarrangement induced by Delta-24-RGD during the autophagic stage, iii) the protein interactomes for Delta-24-RGD-modulated proteome, iv) the regulatory effects on kinase dynamics induced by Delta-24-RGD late in infection, and v) the overproduction of multitasking cytokines upon Delta-24-RGD treatment. We consider that the quantitative molecular maps generated in this study may establish the foundations for the development of complementary adenoviral based-vectors to increase the potency against glioma.

journal_name

J Proteomics

journal_title

Journal of proteomics

authors

González-Morales A,Zabaleta A,García-Moure M,Alonso MM,Fernández-Irigoyen J,Santamaría E

doi

10.1016/j.jprot.2018.11.020

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

168-178

eissn

1874-3919

issn

1876-7737

pii

S1874-3919(18)30418-4

journal_volume

194

pub_type

杂志文章
  • Proteomic alterations induced by ionic liquids in Aspergillus nidulans and Neurospora crassa.

    abstract:UNLABELLED:This study constitutes the first attempt to understand at the proteomic level the fungal response to ionic liquid stress. Ascomycota are able to grow in media supplemented with high concentrations of an ionic liquid, which, in turn, lead to major alterations in the fungal metabolic footprint. Herein, we anal...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2013.09.015

    authors: Martins I,Hartmann DO,Alves PC,Planchon S,Renaut J,Leitão MC,Rebelo LP,Silva Pereira C

    更新日期:2013-12-06 00:00:00

  • Protein methylome analysis in Arabidopsis reveals regulation in RNA-related processes.

    abstract::Protein methylation has been proposed as an important post-translational modification, which occurs predominantly on lysine and arginine residues. Recent discoveries have revealed that protein methylation is also present on non-histones besides histones, and plays critical roles in regulating protein stability and fun...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2019.103601

    authors: Liang Q,Geng Q,Jiang L,Liang M,Li L,Zhang C,Wang W

    更新日期:2020-02-20 00:00:00

  • Assessment of the humoral immune response to cancer.

    abstract::One of the deadly hallmarks of cancer is its ability to prosper within the constraints of the host immune system. Recent advances in immunoproteomics and high-throughput technologies have lead to profiling of the antibody repertoire in cancer patients. This in turn has lead to the identification of tumour associated a...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2012.01.021

    authors: Murphy MA,O'Leary JJ,Cahill DJ

    更新日期:2012-08-03 00:00:00

  • The variations in the nuclear proteome reveal new transcription factors and mechanisms involved in UV stress response in Pinus radiata.

    abstract:UNLABELLED:The importance of UV stress and its side-effects over the loss of plant productivity in forest species demands a deeper understanding of how pine trees respond to UV irradiation. Although the response to UV stress has been characterized at system and cellular levels, the dynamics within the nuclear proteome ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2016.03.003

    authors: Pascual J,Alegre S,Nagler M,Escandón M,Annacondia ML,Weckwerth W,Valledor L,Cañal MJ

    更新日期:2016-06-30 00:00:00

  • Verification of a biomarker discovery approach for detection of Down syndrome in amniotic fluid via multiplex selected reaction monitoring (SRM) assay.

    abstract::Prenatal screening test for Down syndrome (DS) can be improved by discovery of novel biomarkers. A multiplex selected reaction monitoring (SRM) assay was developed to test previously identified thirteen candidate proteins in amniotic fluid (AF). One unique peptide was selected for each protein based on discovery data,...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2011.05.025

    authors: Cho CK,Drabovich AP,Batruch I,Diamandis EP

    更新日期:2011-09-06 00:00:00

  • Glycation of glucose sensitive lysine residues K36, K438 and K549 of albumin is associated with prediabetes.

    abstract::Prediabetes is a risk factor for the development of diabetes. Early diagnosis of prediabetes may prevent the onset and progression of diabetes and its associated complications. Therefore, this study aimed at the identification of novel markers for efficient prediction of prediabetes. In this pursuit, we have evaluated...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2019.103481

    authors: Rathore R,Sonwane BP,Jagadeeshaprasad MG,Kahar S,Santhakumari B,Unnikrishnan AG,Kulkarni MJ

    更新日期:2019-09-30 00:00:00

  • Comparative proteomic analysis of whole saliva from chronic periodontitis patients.

    abstract::Chronic periodontal disease is a chronic inflammatory process affecting tooth supporting tissues in the presence of pathogenic bacterial biofilm. There is some evidence for changes in the protein composition of whole saliva from chronic periodontitis patients, but there have been no studies using a proteomic approach....

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2010.02.018

    authors: Gonçalves Lda R,Soares MR,Nogueira FC,Garcia C,Camisasca DR,Domont G,Feitosa AC,Pereira Dde A,Zingali RB,Alves G

    更新日期:2010-05-07 00:00:00

  • Comparative proteomic and physiological analyses reveal the protective effect of exogenous calcium on the germinating soybean response to salt stress.

    abstract::Calcium enhances salt stress tolerance of soybeans. Nevertheless, the molecular mechanism of calcium's involvement in resistance to salt stress is unclear. A comparative proteomic approach was used to investigate protein profiles in germinating soybeans under NaCl-CaCl2 and NaCl-LaCl3 treatments. A total of 80 protein...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2014.09.023

    authors: Yin Y,Yang R,Han Y,Gu Z

    更新日期:2015-01-15 00:00:00

  • An MRM-based workflow for quantifying cardiac mitochondrial protein phosphorylation in murine and human tissue.

    abstract::The regulation of mitochondrial function is essential for cardiomyocyte adaptation to cellular stress. While it has long been understood that phosphorylation regulates flux through metabolic pathways, novel phosphorylation sites are continually being discovered in all functionally distinct areas of the mitochondrial p...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2012.02.014

    authors: Lam MP,Scruggs SB,Kim TY,Zong C,Lau E,Wang D,Ryan CM,Faull KF,Ping P

    更新日期:2012-08-03 00:00:00

  • Bottom up proteomics reveals novel differentiation proteins in neuroblastoma cells treated with 13-cis retinoic acid.

    abstract::Neuroblastoma, a cancer of the sympathetic nervous system, is the second most common pediatric cancer. A unique feature of neuroblastoma is remission in some patients due to spontaneous differentiation of metastatic tumors. 13-cis retinoic acid (13-cis RA) is currently used in the clinic to treat neuroblastoma due to ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2019.103491

    authors: Halakos EG,Connell AJ,Glazewski L,Wei S,Mason RW

    更新日期:2019-10-30 00:00:00

  • A phosphoproteomic study reveals shp-1 cleavage reprograms LPS signaling via a PI-3K/NF-κB and mTORC1 related mechanism.

    abstract::The reprogrammed lipopolysaccharide (LPS) pathway has been reported to render patients more susceptible to the development of post-traumatic multiple organ dysfunction syndrome (MODS). To facilitate thorough understanding of this mechanism, a phosphoproteomic study was utilized to screen the potential signaling molecu...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2015.07.003

    authors: Xu E,Chen J,Wang Y,Ke Z,Luo S,Zou Z

    更新日期:2015-10-14 00:00:00

  • Deep learning embedder method and tool for mass spectra similarity search.

    abstract::Spectral similarity calculation is widely used in protein identification tools and mass spectra clustering algorithms while comparing theoretical or experimental spectra. The performance of the spectral similarity calculation plays an important role in these tools and algorithms especially in the analysis of large-sca...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2020.104070

    authors: Qin C,Luo X,Deng C,Shu K,Zhu W,Griss J,Hermjakob H,Bai M,Perez-Riverol Y

    更新日期:2021-02-10 00:00:00

  • Intrinsic disorder and multiple phosphorylations constrain the evolution of the flightin N-terminal region.

    abstract::Flightin is a myosin binding phosphoprotein that originated in the ancestor to Pancrustacea ~500 MYA. In Drosophila melanogaster, flightin is essential for length determination and flexural rigidity of thick filaments. Here, we show that among 12 Drosophila species, the N-terminal region is characterized by low sequen...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2015.12.006

    authors: Lemas D,Lekkas P,Ballif BA,Vigoreaux JO

    更新日期:2016-03-01 00:00:00

  • Quantitative proteomics analysis by iTRAQ revealed underlying changes in thermotolerance of Arthrospira platensis.

    abstract::Growth temperature is a critical factor that affects cultivation of Arthrospira platensis which is a type of cyanobacterium widely known as Spirulina that has significant commercial value. To investigate the molecular mechanism underlying the thermotolerance of Spirulina, differential protein expression profiling was ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2017.06.015

    authors: Chang R,Lv B,Li B

    更新日期:2017-08-08 00:00:00

  • Intraspecies variation in the venom of the rattlesnake Crotalus simus from Mexico: different expression of crotoxin results in highly variable toxicity in the venoms of three subspecies.

    abstract:UNLABELLED:The composition and toxicological profile of the venom of the rattlesnake Crotalus simus in Mexico was analyzed at the subspecies and individual levels. Venoms of the subspecies C. s. simus, C. s. culminatus and C. s. tzabcan greatly differ in the expression of the heterodimeric neurotoxin complex 'crotoxin'...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2013.05.024

    authors: Castro EN,Lomonte B,del Carmen Gutiérrez M,Alagón A,Gutiérrez JM

    更新日期:2013-07-11 00:00:00

  • Twenty years of protein interaction studies for biological function deciphering.

    abstract::Intensive methodological developments and technology innovation have been devoted to protein-protein interaction studies over 20years. Genetic indirect assays and sophisticated large scale biochemical analyses have jointly contributed to the elucidation of protein-protein interactions, still with a lot of drawbacks de...

    journal_title:Journal of proteomics

    pub_type: 历史文章,杂志文章,评审

    doi:10.1016/j.jprot.2014.03.038

    authors: Legrain P,Rain JC

    更新日期:2014-07-31 00:00:00

  • RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.

    abstract::Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensi...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2015.07.029

    authors: Bi Z,Merl-Pham J,Uehlein N,Zimmer I,Mühlhans S,Aichler M,Walch AK,Kaldenhoff R,Palme K,Schnitzler JP,Block K

    更新日期:2015-10-14 00:00:00

  • Plant proteomics update (2007-2008): Second-generation proteomic techniques, an appropriate experimental design, and data analysis to fulfill MIAPE standards, increase plant proteome coverage and expand biological knowledge.

    abstract::This review is the continuation of three previously published articles [Jorrin JV, Maldonado AM, Castillejo MA. Plant proteome analysis: a 2006 update. Proteomics 2007; 7: 2947-2962; Rossignol M, Peltier JB, Mock HP, Matros A, Maldonado AM, Jorrin JV. Plant proteome analysis: a 2004-2006 update. Proteomics 2006; 6: 55...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2009.01.026

    authors: Jorrín-Novo JV,Maldonado AM,Echevarría-Zomeño S,Valledor L,Castillejo MA,Curto M,Valero J,Sghaier B,Donoso G,Redondo I

    更新日期:2009-04-13 00:00:00

  • Dynamic remodeling of the interactomes of Nematostella vectensis Hsp70 isoforms under heat shock.

    abstract::Heat shock protein 70s (Hsp70s) are a highly conserved class of molecular chaperones that fold a large proportion of the proteome. Nematostella vectensis (Nv) is an estuarine sea anemone that has emerged as a model species to characterize molecular responses to physiological stressors due to its exposure to diverse, e...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2019.103416

    authors: Knighton LE,Nitika,Waller SJ,Strom O,Wolfgeher D,Reitzel AM,Truman AW

    更新日期:2019-08-30 00:00:00

  • The role of heat stress on the age related protein carbonylation.

    abstract:UNLABELLED:Since the proteins are involved in many physiological processes in the organisms, modifications of proteins have important outcomes. Protein modifications are classified in several ways and oxidative stress related ones take a wide place. Aging is characterized by the accumulation of oxidized proteins and de...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2013.06.025

    authors: Bozaykut P,Sozen E,Kaga E,Ece A,Ozaltin E,Ek B,Ozer NK,Grune T,Bergquist J,Karademir B

    更新日期:2013-08-26 00:00:00

  • Proteomics survey of Solanaceae family: Current status and challenges ahead.

    abstract::Solanaceae is one of the major economically important families of higher plants and has played a central role in human nutrition since the dawn of human civilization. Therefore, researchers have always been interested in understanding the complex behavior of Solanaceae members to identify key transcripts, proteins or ...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2017.05.016

    authors: Ghatak A,Chaturvedi P,Paul P,Agrawal GK,Rakwal R,Kim ST,Weckwerth W,Gupta R

    更新日期:2017-10-03 00:00:00

  • Cellular proteome alterations in response to enterovirus 71 and coxsackievirus A16 infections in neuronal and intestinal cell lines.

    abstract::Hand, foot and mouth disease is mainly caused by enterovirus A71 (EV-A71) and coxsackievirus A16 (CV-A16), but EV-A71 is also associated with severe neurological complications. Host factors may contribute to the different clinical outcomes of EV-A71 and CV-A16 infections. A neurovirulent EV-A71 strain (EV-A71/UH1) fro...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2015.05.016

    authors: Chan SY,Sam IC,Lai JK,Chan YF

    更新日期:2015-07-01 00:00:00

  • The use of omics profiling to improve outcomes of bone regeneration and osseointegration. How far are we from personalized medicine in dentistry?

    abstract::Increased life expectancy and broader restorative dental treatment alternatives for missing teeth have resulted in an increasing request of bone regeneration/augmentation procedures not only in healthy patients, but also in elderly and medically compromised ones. This is also combined with a growing demand for short i...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2018.01.017

    authors: Calciolari E,Donos N

    更新日期:2018-09-30 00:00:00

  • Leaf proteome profiling of transgenic mint infected with Alternaria alternata.

    abstract:UNLABELLED:The genus Mentha has been widely used in food, flavor, culinary, cosmetic and pharmaceutical industries. Substantial damage to this crop happened regularly due to environmental stresses like metal toxicity and pathogen attack. Here, an approach has been taken to raise transgenic mint over-expressing γ-glutam...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2013.01.020

    authors: Sinha R,Bhattacharyya D,Majumdar AB,Datta R,Hazra S,Chattopadhyay S

    更新日期:2013-11-20 00:00:00

  • Proteomic analysis of porcine alveolar macrophages infected with porcine circovirus type 2.

    abstract::Post-weaning multi-systemic wasting syndrome (PMWS), which is primarily caused by porcine circovirus type 2 (PCV2), is an emerging swine immunosuppressive disease. To characterize the interaction between target immune cells and PCV2, the differential proteomes of porcine alveolar macrophages, with and without PCV2 inf...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2012.03.039

    authors: Cheng S,Zhang M,Li W,Wang Y,Liu Y,He Q

    更新日期:2012-06-18 00:00:00

  • Comparative proteomic analysis of four Bacillus clausii strains: proteomic expression signature distinguishes protein profile of the strains.

    abstract::A comparative proteomic approach, using two dimensional gel electrophoresis and mass spectrometry, has been developed to compare and elucidate the differences among the cellular proteomes of four closely related isogenic O/C, SIN, N/R and T, B. clausii strains during both exponential and stationary phases of growth. I...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2011.06.032

    authors: Lippolis R,Gnoni A,Abbrescia A,Panelli D,Maiorano S,Paternoster MS,Sardanelli AM,Papa S,Gaballo A

    更新日期:2011-11-18 00:00:00

  • Method and platform standardization in MRM-based quantitative plasma proteomics.

    abstract:UNLABELLED:There exists a growing demand in the proteomics community to standardize experimental methods and liquid chromatography-mass spectrometry (LC/MS) platforms in order to enable the acquisition of more precise and accurate quantitative data. This necessity is heightened by the evolving trend of verifying and va...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2013.07.026

    authors: Percy AJ,Chambers AG,Yang J,Jackson AM,Domanski D,Burkhart J,Sickmann A,Borchers CH

    更新日期:2013-12-16 00:00:00

  • Guidelines for reporting quantitative mass spectrometry based experiments in proteomics.

    abstract:UNLABELLED:Mass spectrometry is already a well-established protein identification tool and recent methodological and technological developments have also made possible the extraction of quantitative data of protein abundance in large-scale studies. Several strategies for absolute and relative quantitative proteomics an...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2013.02.026

    authors: Martínez-Bartolomé S,Deutsch EW,Binz PA,Jones AR,Eisenacher M,Mayer G,Campos A,Canals F,Bech-Serra JJ,Carrascal M,Gay M,Paradela A,Navajas R,Marcilla M,Hernáez ML,Gutiérrez-Blázquez MD,Velarde LF,Aloria K,Beaskoetxea

    更新日期:2013-12-16 00:00:00

  • Deciphering the molecular mechanisms underlying sea urchin reversible adhesion: A quantitative proteomics approach.

    abstract:UNLABELLED:Marine bioadhesives have unmatched performances in wet environments, being an inspiration for biomedical applications. In sea urchins specialized adhesive organs, tube feet, mediate reversible adhesion, being composed by a disc, producing adhesive and de-adhesive secretions, and a motile stem. After tube foo...

    journal_title:Journal of proteomics

    pub_type: 杂志文章

    doi:10.1016/j.jprot.2016.02.026

    authors: Lebesgue N,da Costa G,Ribeiro RM,Ribeiro-Silva C,Martins GG,Matranga V,Scholten A,Cordeiro C,Heck AJ,Santos R

    更新日期:2016-04-14 00:00:00

  • Titanium dioxide as chemo-affinity chromatographic sorbent of biomolecular compounds--applications in acidic modification-specific proteomics.

    abstract::Characterization of the biomolecules involved in molecular processes occurring in biological systems such as the human cell remains central to biology, biotechnology, and medicine. One of the preferred methods of selectively purifying specific classes of biomolecules from complex biological matrices for further charac...

    journal_title:Journal of proteomics

    pub_type: 杂志文章,评审

    doi:10.1016/j.jprot.2011.07.024

    authors: Engholm-Keller K,Larsen MR

    更新日期:2011-12-21 00:00:00