MicroRNA-543 promotes ovariectomy-induced osteoporosis through inhibition of AKT/p38 MAPK signaling pathway by targeting YAF2.


:The present study aimed to determine the roles of miRNA-543 in osteoporosis in rats induced by ovariectomy. The osteoporosis rat model was established by ovariectomy induction. MiRNA-543 expression in osteoblasts was measured by quantitative real-time polymerase chain reaction. The cell proliferation and apoptosis were measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry assays, respectively. Western blot analysis was conducted to examine the expression of YAF-2 and AKT signaling. TargetScan analysis and dual-luciferase reporter assay were performed to determine the target gene of miRNA-543. MiRNA-543 was significantly upregulated in osteoporosis rat model. Overexpression of miRNA-543 significantly suppressed cell growth and promoted apoptosis in osteoblasts, whereas downregulation of miRNA-543 significantly enhanced cell growth and inhibited apoptosis. MiRNA-543 upregulation significantly inhibited YAF-2 expression and suppressed the phosphorylation and expression of AKT and p38 mitogen-activated protein kinases (MAPK) in osteoblasts. Furthermore, YAF-2 knockdown enhanced the effects of miRNA-543 on apoptosis in osteoblasts. AKT inhibitor MK2206 and p38 MAPK inhibitor SB203580 also enhanced the effects of miRNA-543 on apoptosis in osteoblasts. Our findings revealed that inhibition of miRNA-543 could protect osteoblasts against ovariectomy-induced osteoporosis through AKT/p38 MAPK signaling pathway by targeting YAF2.


J Cell Biochem


Li X,Ning L,Zhao X,Wan S




Has Abstract


2018-12-02 00:00:00






  • Identification of an IQGAP1/AKAP79 complex in beta-cells.

    abstract::IQGAP1, is a recently discovered scaffold protein proposed to regulate membrane cytoskeleton events through protein-protein interactions with F-actin, E-cadherin, beta-catenin, and CLIP170. The binding of IQGAP1 to its partners is regulated by calcium/calmodulin (Ca(++)/CaM) and the small molecular weight guanine nucl...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Nauert JB,Rigas JD,Lester LB

    更新日期:2003-09-01 00:00:00

  • Isolation and characterization of squamous carcinoma cells resistant to photodynamic therapy.

    abstract::Photodynamic therapy (PDT) employing methyl δ-aminolevulinic acid (Me-ALA), as a precursor of the photosensitizer protoporphyrin IX (PpIX), is used for the treatment of non melanoma cutaneous cancer (NMCC). However, one of the problems of PDT is the apparition of resistant cell populations. The aim of this study was t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Milla LN,Cogno IS,Rodríguez ME,Sanz-Rodríguez F,Zamarrón A,Gilaberte Y,Carrasco E,Rivarola VA,Juarranz A

    更新日期:2011-09-01 00:00:00

  • Potentiation of cancerous progression by LISCH7 via direct stimulation of TGFB1 transcription in triple-negative breast cancer.

    abstract::As an aggressive breast cancer (BCa) subtype, triple-negative breast cancer (TNBC) responses poorly to chemotherapy and endocrine therapy, and usually has a worse prognosis. This is largely due to the lack of specific therapeutic targets, laying claim to an imperious demand to clarify the key signaling pathways potent...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Tang X,Zhou Y,Liu Y,Zhang W,Liu C,Yan C

    更新日期:2020-11-01 00:00:00

  • Reversal of BCRP-mediated multidrug resistance by stable expression of small interfering RNAs.

    abstract::Breast cancer resistance protein (BCRP) is an ATP-binding cassette multidrug transporter that confers resistance to various anticancer drugs like Mitoxantrone. Overexpression of BCRP confers multidrug resistance (MDR) in cancer cells and is a frequent impediment to successful chemotherapy. For stable reversal of BCRP-...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Lv H,He Z,Liu X,Yuan J,Yu Y,Chen Z

    更新日期:2007-09-01 00:00:00

  • Tissue crosstalk in lung development.

    abstract::Lung development follows a stereotypic program orchestrated by key interactions among epithelial and mesenchymal tissues. Deviations from this developmental program can lead to pulmonary diseases including bronchopulmonary dysplasia and pulmonary hypertension. Significant efforts have been made to examine the cellular...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Hines EA,Sun X

    更新日期:2014-09-01 00:00:00

  • Transforming growth factor-beta 1 regulation of bone sialoprotein gene transcription: identification of a TGF-beta activation element in the rat BSP gene promoter.

    abstract::Transforming growth factor-beta (TGF-beta) increases steady-state mRNA levels of several extracellular matrix proteins in mineralized connective tissues. Bone sialoprotein (BSP) is a major constituent of the bone matrix, thought to initiate and regulate the formation of mineral crystals. To determine the molecular pat...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Ogata Y,Niisato N,Furuyama S,Cheifetz S,Kim RH,Sugiya H,Sodek J

    更新日期:1997-06-15 00:00:00

  • Reveromycin A-Induced Apoptosis in Osteoclasts Is Not Accompanied by Necrosis.

    abstract::Reveromycin A (RM-A), a small natural product isolated from Streptomyces bacteria, is a potential osteoporosis therapeutic in that it specifically induces apoptosis in osteoclasts but not osteoblasts. The purpose of the study presented here was to further elucidate the intracellular mechanisms of RM-A death effects in...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Mead B,Morgan H,Mann-Knowlton A,Tedeschi L,Sloan C,Lang S,Hines C,Gragg M,Stofer J,Riemann K,Derr T,Heller E,Collins D,Landis P,Linna N,Jones D

    更新日期:2015-08-01 00:00:00

  • Multi-color palette of fluorescent proteins for imaging the tumor microenvironment of orthotopic tumorgraft mouse models of clinical pancreatic cancer specimens.

    abstract::Pancreatic-cancer-patient tumor specimens were initially established subcutaneously in NOD/SCID mice immediately after surgery. The patient tumors were then harvested from NOD/SCID mice and passaged orthotopically in transgenic nude mice ubiquitously expressing red fluorescent protein (RFP). The primary patient tumors...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Suetsugu A,Katz M,Fleming J,Truty M,Thomas R,Moriwaki H,Bouvet M,Saji S,Hoffman RM

    更新日期:2012-07-01 00:00:00

  • Modulation of the ERK pathway of signal transduction by cysteine proteinase inhibitors.

    abstract::Cell proliferation requires the coordinate synthesis and degradation of many proteins. In addition to the well-characterized involvement of the proteasome in the degradation of several cell cycle-regulated proteins, it has been established that cysteine proteinases are also involved in the control of cell proliferatio...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Torres C,Li M,Walter R,Sierra F

    更新日期:2000-09-18 00:00:00

  • Are primary cultures realistic models of prostate cancer?

    abstract::Primary cultures fill a unique niche among the repertoire of in vitro model systems available to investigate the biology of the normal and malignant human prostate. This review summarizes some of the properties of primary cultures, with special emphasis on two questions: are primary cultures from adenocarcinomas reall...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Peehl DM

    更新日期:2004-01-01 00:00:00

  • Down regulation of miR200c promotes radiation-induced thymic lymphoma by targeting BMI1.

    abstract::The miR-200c has recently been implicated in the epithelial to mesenchymal transition (EMT) process by directly target the EMT related transcriptional factors ZEB1 and ZEB2. The expression of this miRNA is inversely correlated with tumorgenecity and invasiveness in several human cancers. However, little is known about...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Cui J,Cheng Y,Zhang P,Sun M,Gao F,Liu C,Cai J

    更新日期:2014-06-01 00:00:00

  • Phosphorylation of the insulin receptor by casein kinase I.

    abstract::Insulin receptor was examined as a substrate for the multipotential protein kinase casein kinase I. Casein kinase I phosphorylated partially purified insulin receptor from human placenta as shown by immunoprecipitation of the complex with antiserum to the insulin receptor. Analysis of the phosphorylated complex by pol...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Tuazon PT,Pang DT,Shafer JA,Traugh JA

    更新日期:1985-01-01 00:00:00

  • Mitochondrial inhibitory factor protein 1 attenuates coupling factor 6-induced aging signal.

    abstract::Coupling factor 6 (CF6) forces a counter-clockwise rotation of plasma membrane F1 Fo complex, resulting in proton import and accelerated aging. Inhibitory factor peptide 1 (IF1) suppresses a unidirectional counter-clockwise rotation of F1 Fo complex without affecting ATP synthesis. We tested the hypothesis that IF1 ma...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Osanai T,Tanaka M,Mikami K,Kitajima M,Magota K,Tomita H,Okumura K

    更新日期:2018-07-01 00:00:00

  • Role of immune system in tumor progression and carcinogenesis.

    abstract::Tumor micro-environment has potential to customize the behavior of the immune cell according to their need. In immune-eliminating phase, immune cells eliminate transformed cells but after tumor establishment innate and adaptive immune cells synergistically provide shelter as well as fulfill their requirement that help...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Upadhyay S,Sharma N,Gupta KB,Dhiman M

    更新日期:2018-07-01 00:00:00

  • Atorvastatin stimulates the production of osteoprotegerin by human osteoblasts.

    abstract::Recently, HMG-CoA reductase inhibitors (statins), potent inhibitors of cholesterol biosynthesis, have been linked to protective effects on bone metabolism. Because of their widespread use, prevention of bone loss and fractures would be a desirable side effect. However, the mechanisms how statins may affect bone metabo...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Viereck V,Gründker C,Blaschke S,Frosch KH,Schoppet M,Emons G,Hofbauer LC

    更新日期:2005-12-15 00:00:00

  • HOXB13 promotes proliferation, migration, and invasion of glioblastoma through transcriptional upregulation of lncRNA HOXC-AS3.

    abstract::HOXB13 exerts a close relation in several human cancers. This study explored the role of HOXB13 in glioblastoma (GBM), a brain tissue with the highest aggressive rate and mortality in adults. Through microarray and immunohistochemistry analyses, HOXB13 was highly expressed in GBM tissues. Furthermore, we showed that h...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wang X,Sun Y,Xu T,Qian K,Huang B,Zhang K,Song Z,Qian T,Shi J,Li L

    更新日期:2019-09-01 00:00:00

  • Lactosylceramide-Induced Phosphorylation Signaling to Group IVA Phospholipase A2 via Reactive Oxygen Species in Tumor Necrosis Factor-α-Treated Cells.

    abstract::The activity of α-type cytosolic phospholipase A2 (cPLA2 α, group IVA PLA2 ), which releases arachidonic acid (AA), is mainly regulated by the Ca2+ -induced intracellular translocation/attachment of the enzyme to substrate membranes and its phosphorylation. We previously reported that tumor necrosis factor-α (TNFα) st...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Nakamura H,Moriyama Y,Watanabe K,Tomizawa S,Yamazaki R,Takahashi H,Murayama T

    更新日期:2017-12-01 00:00:00

  • miR-21 downregulates the tumor suppressor P12 CDK2AP1 and stimulates cell proliferation and invasion.

    abstract::The present study was undertaken to investigate the regulation of P12(CDK2AP1) by miRNAs. A conserved target site for miR-21 within the CDK2AP1-3'-UTR at nt 349-370 was predicted by bioinformatics software and an inverse correlation of miR-21 and CDK2AP1 protein was observed. Highly specific amplification and quantifi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zheng J,Xue H,Wang T,Jiang Y,Liu B,Li J,Liu Y,Wang W,Zhang B,Sun M

    更新日期:2011-03-01 00:00:00

  • Timely Degradation of Wip1 Phosphatase by APC/C Activator Protein Cdh1 is Necessary for Normal Mitotic Progression.

    abstract::Wip1 belongs to the protein phosphatase C (PP2C) family, of which expression is up-regulated by a number of external stresses, and serves as a stress modulator in normal physiological conditions. When overexpressed, premature dephosphorylation of stress-mediators by Wip1 results in abrogation of tumor surveillance, th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Jeong HC,Gil NY,Lee HS,Cho SJ,Kim K,Chun KH,Cho H,Cha HJ

    更新日期:2015-08-01 00:00:00

  • Free Fatty Acids Induce Autophagy and LOX-1 Upregulation in Cultured Aortic Vascular Smooth Muscle Cells.

    abstract::Elevation of free fatty acids (FFAs) is known to affect microvascular function and contribute to obesity-associated insulin resistance, hypertension, and microangiopathy. Proliferative and synthetic vascular smooth muscle cells (VSMCs) increase intimal thickness and destabilize atheromatous plaques. This study aimed t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Cheng CI,Lee YH,Chen PH,Lin YC,Chou MH,Kao YH

    更新日期:2017-05-01 00:00:00

  • Functional analysis of DNA sequences located within a cluster of DNase I hypersensitive sites colocalizing with a MAR element at the upstream border of the chicken alpha-globin gene domain.

    abstract::We have cloned and sequenced a genomic DNA fragment of chicken containing a cluster of DNase I hypersensitive sites (DHS) located 11-15 kb upstream from the first gene of the alpha-globin gene domain and including a constitutive DHS flanked by two erythroid-specific ones. A 1.2-kb subfragment of the DNA fragment under...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Razin SV,Shen K,Ioudinkova E,Scherrer K

    更新日期:1999-07-01 00:00:00

  • Phospholipase C-γ2 promotes intracellular survival of mycobacteria.

    abstract::Mycobacterium tuberculosis (Mtb) infects millions of people each year. These bacilli can survive inside macrophages. To favor their survival, pathogen alters various signal transduction pathways in host cells. Phospholipase C (PLC) signaling regulates various processes in mammalian cells but has never been investigate...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Paroha R,Chaurasiya SK,Chourasia R

    更新日期:2019-04-01 00:00:00

  • Cellular Migration Ability Is Modulated by Extracellular Purines in Ovarian Carcinoma SKOV-3 Cells.

    abstract::Extracellular nucleotides and nucleosides have emerged as important elements regulating tissue homeostasis. Acting through specific receptors, have the ability to control gene expression patterns to direct cellular fate. We observed that SKOV-3 cells express the ectonucleotidases: ectonucleotide pyrophosphatase 1 (ENP...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Martínez-Ramírez AS,Díaz-Muñoz M,Battastini AM,Campos-Contreras A,Olvera A,Bergamin L,Glaser T,Jacintho Moritz CE,Ulrich H,Vázquez-Cuevas FG

    更新日期:2017-12-01 00:00:00

  • ACVR1-knockout promotes osteogenic differentiation by activating the Wnt signaling pathway in mice.

    abstract::Osteogenic differentiation refers to the process of bone formation and remodeling, which is controlled by complex molecular mechanisms. Activin A receptor type I (ACVR1) is reported to be associated with osteogenic differentiation. However, the underlying molecular mechanism remains elusive. Therefore, this study eval...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wang Y,Sun JC,Wang HB,Xu XM,Kong QJ,Wang YJ,Zheng B,Shi GD,Shi JG

    更新日期:2018-12-16 00:00:00

  • Proteomic analysis of the function of spot in Helicobacter pylori anti-oxidative stress in vitro and colonization in vivo.

    abstract::As a microaerobe, Helicobacter pylori employs the global regulator SpoT for defending against oxidative stress in vitro. However, the mechanisms how SpoT affects bacterial gene expression is still unknown. Moreover, the function of SpoT in H. pylori colonization in the host is remaining undetermined. To explore the fu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sun Y,Li X,Li W,Zhao M,Wang L,Liu S,Zeng J,Liu Z,Jia J

    更新日期:2012-11-01 00:00:00

  • High expression of SGTA in esophageal squamous cell carcinoma correlates with proliferation and poor prognosis.

    abstract::Receptor tyrosine kinases (RTKs) expression and the growth factor such as platelet-derived growth factor (PDGF) and their receptors have been considered relevant in the process of angiogenesis and dissemination in esophageal squamous cell carcinoma (ESCC). Small glutamine-rich tetratricopeptide repeat-containing prote...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yang X,Cheng L,Li M,Shi H,Ren H,Ding Z,Liu F,Wang Y,Cheng C

    更新日期:2014-01-01 00:00:00

  • The role of membrane lectins in Dictyostelium discoideum aggregation as ascertained by specific univalent antibodies against discoidin I.

    abstract::Antibodies against pure discoidin I have been used as a tool to ascertain the role of this lectin in aggregation of Dictyostelium discoideum. Discoidin I is widely expressed over the cell surface of aggregation-competent AX-2 cells, as ascertained by indirect immunofluorescence with specific (antidiscoidin I) antibodi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Cano A,Pestaña A

    更新日期:1984-01-01 00:00:00

  • Methylation of the 5' flanking sequences of the ribosomal DNA in human cell lines and in a human-hamster hybrid cell line.

    abstract::In a human lymphoblastoid cell line (Z83) in which rDNA genes on chromosome 22 are amplified but transcribed at a low level, immunocytological studies with antibodies to 5 methylcytidine provided evidence for hypermethylation of the rDNA. The extent of methylation of the 5' flanking sequences of the ribosomal DNA was ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Dante R,Percy ME,Baldini A,Markovic VD,Miller DA,Rocchi M,Niveleau A,Miller OJ

    更新日期:1992-12-01 00:00:00

  • Endothelin modulates osteopontin and osteocalcin messenger ribonucleic acid expression in rat osteoblastic osteosarcoma cells.

    abstract::Endothelins (ETs) are vasoconstrictive peptides produced mainly by endothelial cells. The ET receptors are expressed in many types of cells including osteoblast-like cells. The purpose of this study was to examine the effects of endothelin on the expression of osteoblastic phenotype-related genes. We found that endoth...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Shioide M,Noda M

    更新日期:1993-10-01 00:00:00

  • Computational and mutational analysis of TatD DNase of Bacillus anthracis.

    abstract::The role of TatD DNases as DNA repair enzymes or cell death (apoptotic) nucleases is well established in prokaryotes as well as eukaryotes. The current study aims to characterize the TatD nuclease from Bacillus anthracis (Ba TatD) and to explore its key histidine catalytic residues. Ba TatD was found to be a metal-dep...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Singh D,Rahi A,Kumari R,Gupta V,Gautam G,Aggarwal S,Rehan M,Bhatnagar R

    更新日期:2019-02-05 00:00:00