Amputation-Site Soft-Tissue Restoration Using Adipose Stem Cell Therapy.


:Soft-tissue deficits in amputation stumps can lead to significant pain and disability. An emerging treatment option is stem cell-enriched fat grafting. This is the first study assessing the potential for this treatment modality in lower extremity amputation sites. In this prospective cohort study, five injured military personnel suffering from pain and limited function at amputation sites were recruited. Fat grafting enriched with stromal vascular fraction was performed at amputation sites to provide additional subcutaneous tissue padding over bony structures. Outcomes measures included complications, demographic data, physical examination, cellular subpopulations, cell viability, graft volume retention, pain, Lower Extremity Functional Scale, Functional Mobility Assessment, 36-Item Short-Form Health Survey, and rates of depression. Follow-up was 2 years. There were no significant complications. Volume retention was 61.5 ± 24.0 percent. Overall cell viability of the stromal vascular fraction was significantly correlated with volume retention (p = 0.016). There was no significant correlation between percentage of adipose-derived stem cells or number of cells in the stromal vascular fraction and volume retention. There was a nonsignificant trend toward improvement in pain scores (3.0 ± 2.5 to 1.2 ± 1.6; p = 0.180 at 2 years). There were no significant changes in disability indexes. Results from this pilot study demonstrate that stromal vascular fraction-enriched fat grafting is a safe, novel modality for the treatment of symptomatic soft-tissue defects in traumatic lower extremity amputations. Volume retention can be anticipated at slightly over 60 percent. Further studies are needed to assess efficacy. CLINICAL QUESTION/LEVEL OF EVIDENCE:: Therapeutic, IV.


Plast Reconstr Surg


Bourne DA,Thomas RD,Bliley J,Haas G,Wyse A,Donnenberg A,Donnenberg VS,Chow I,Cooper R,Coleman S,Marra K,Pasquina PF,Rubin JP




Has Abstract


2018-11-01 00:00:00














  • Optimal axon counts for brachial plexus nerve transfers to restore elbow flexion.

    abstract:BACKGROUND:Nerve transfer surgery has revolutionized the management of traumatic brachial plexus injures. However, the optimal size ratio of donor to recipient nerve has yet to be elucidated. The authors investigated the axon count ratios of ulnar and median fascicular transfers to restore elbow flexion. The authors hy...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Schreiber JJ,Byun DJ,Khair MM,Rosenblatt L,Lee SK,Wolfe SW

    更新日期:2015-01-01 00:00:00

  • Evidence-based medicine: cleft palate.

    abstract:LEARNING OBJECTIVES:After reading this article, the participant should be able to: 1. Describe recent changes in treatment of cleft palate. 2. Compare the efficacy of different surgical treatments. 3. Assess their own knowledge of cleft palate repair. 4. Determine where further individual in-depth study and development...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Chepla KJ,Gosain AK

    更新日期:2013-12-01 00:00:00

  • Digital two-dimensional photogrammetry: a comparison of three techniques of obtaining digital photographs.

    abstract::Three methods of obtaining digital photographs in a clinical setting were compared using direct anthropometry as a reference standard. The methods included a digital camera, scanning negatives from a 35-mm single-lens reflex camera, and scanning Polaroid photographs taken with a Polaroid camera designed for medical do...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Nechala P,Mahoney J,Farkas LG

    更新日期:1999-06-01 00:00:00

  • Nasolabial flap for nasal tip reconstruction.

    abstract:BACKGROUND:The nasolabial flap is a pedicled flap with a wide description and application for use in lateral nasal wall, ala, columella, and intraoral reconstruction. It is a versatile flap that is well tolerated by the patient, with very acceptable donor-site scars. The soft and spongy nature of the flap, with its pro...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Thornton JF,Weathers WM

    更新日期:2008-09-01 00:00:00

  • The operative treatment of isolated craniofacial dysostosis (plagiocephaly): a comparison of the unilateral and bilateral techniques.

    abstract::Both the safety and efficacy of the treatment of isolated craniofacial dysostosis (plagiocephaly) in infancy have been demonstrated. Opinions remain divided, however, as to the optimal type of procedure to be undertaken. In an attempt to answer this question, we have retrospectively evaluated a study population of 48 ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Bartlett SP,Whitaker LA,Marchac D

    更新日期:1990-05-01 00:00:00

  • Does breast reconstruction after mastectomy for breast cancer affect overall survival? Long-term follow-up of a retrospective population-based cohort.

    abstract:BACKGROUND:This study compared overall and breast cancer-specific survival using long-term follow-up data among women diagnosed with invasive breast cancer undergoing mastectomy or breast reconstruction. METHODS:Retrospective study using population-based data from Ontario Cancer Registry (1980 to 1990) including women...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,多中心研究


    authors: Platt J,Baxter NN,McLaughlin J,Semple JL

    更新日期:2015-03-01 00:00:00

  • Recurrent ventral herniation in Ehlers-Danlos syndrome.

    abstract::Ehlers-Danlos syndrome is an inherited collagen disorder characterized by skin hyperextensibility, joint laxity, and tissue friability. In this study, it was hypothesized that Ehlers-Danlos syndrome is frequently undiagnosed in patients who present for repair of ventral abdominal wall hernias. A retrospective chart re...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Girotto JA,Malaisrie SC,Bulkely G,Manson PN

    更新日期:2000-12-01 00:00:00

  • Comparison of rapid versus slow tissue expansion on skin-flap viability.

    abstract::The effect of rapid versus slow tissue expansion on random-pattern skin-flap survival lengths was evaluated in two groups of pigs. Each group (group 1--slow; group 2--rapid) was further evaluated for the effect that delay (A), delay plus expansion (B), expansion only (C), and acute flap elevation (D) had on flap lengt...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Schneider MS,Wyatt DB,Konvolinka CW,Hassanein KM,Hiebert JM

    更新日期:1993-11-01 00:00:00

  • The axial nasodorsum flap.

    abstract::A new axial flap is described for reconstruction of soft-tissue defects of the nose, including the supratip and columella. The blood supply to this flap originates from the lateral nasal branch of the angular artery adjacent to the nasolabial fold. This flap was used in seven patients between 1989 and 1994, and there ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Maruyama Y,Iwahira Y

    更新日期:1997-06-01 00:00:00

  • Indications and Limitations of Bilayer Wound Matrix-Based Lower Extremity Reconstruction: A Multidisciplinary Case-Control Study of 191 Wounds.

    abstract:BACKGROUND:Little is known about the efficacy of newer skin substitute scaffolds to reconstruct complex lower extremity wounds. The investigators present a multihospital experience of reconstructive surgeons utilizing collagen-GAG bilayer wound matrix in lower extremity soft-tissue reconstruction with the goals to (1) ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Shakir S,Messa CA 4th,Broach RB,Rhemtulla IA,Chatman B,D'Angelantonio A,Levin LS,Kovach SJ 3rd,Serletti JM,Fischer JP

    更新日期:2020-03-01 00:00:00

  • Interventions to Decrease Postoperative Edema and Ecchymosis after Rhinoplasty: A Systematic Review of the Literature.

    abstract:BACKGROUND:Today, minimally invasive procedures are becoming more popular because of the fast recovery. Rhinoplasty is a common facial plastic surgery procedure that can be associated with significant postoperative morbidities, especially periorbital edema and ecchymosis. The aim of this review is to summarize the resu...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Ong AA,Farhood Z,Kyle AR,Patel KG

    更新日期:2016-05-01 00:00:00

  • Post-circumcision epidermoid inclusion cysts of the clitoris.

    abstract::We describe our experiences during the last 5 years with 19 cases of epidermoid inclusion cysts of the clitoris resulting from circumcision. There were 14 children and 5 adults. The management consisted of complete excision of the cyst, and reconstruction of the clitoris, wherever possible. ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Ofodile FA,Oluwasanmi JO

    更新日期:1979-04-01 00:00:00

  • Gastrocnemius transposition flap in conjunction with limb-sparing surgery for primary bone sarcomas around the knee.

    abstract::A primary gastrocnemius transposition flap is a useful technique for prosthetic coverage following extensive soft-tissue and bone resection for sarcomas of the knee joint. The gastrocnemius transposition flap is also a useful secondary procedure to treat local complications following attempted limb-sparing surgery. Th...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Malawer MM,Price WM

    更新日期:1984-05-01 00:00:00

  • Unpredictable growth pattern of costochondral graft.

    abstract::Costochondral grafts have gained increasing popularity in reconstruction of the temporomandibular joint and condyle in children. This is a report on the long-term follow-up of eight adolescent patients who underwent reconstruction of the temporomandibular joint and ramus for correction of hemifacial microsomia or trau...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Guyuron B,Lasa CI Jr

    更新日期:1992-11-01 00:00:00

  • Thin anterolateral thigh perforator flap using a modified perforator microdissection technique and its clinical application for foot resurfacing.

    abstract:BACKGROUND:A thin skin flap is often required for optimal resurfacing of particular areas of the body. An anterolateral thigh perforator flap can be thinned to an extent to which it is vascularized by the subdermal plexus only. This study presents a novel flap thinning technique and its application for resurfacing the ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Yang WG,Chiang YC,Wei FC,Feng GM,Chen KT

    更新日期:2006-03-01 00:00:00

  • Comparison of the rotation-advancement and philtral ridge techniques for unilateral cleft lip repair.

    abstract:BACKGROUND:The Millard rotation-advancement flap has become the most widely used technique for unilateral cleft lip repair. The philtral ridge repair is a modified straight-line technique that was developed to further optimize the scar associated with the rotation-advancement flap. The purpose of this article is to int...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Cline JM,Oyer SL,Javidnia H,Nguyen SA,Sykes JM,Kline RM,Patel KG

    更新日期:2014-12-01 00:00:00

  • Benign and premalignant skin lesions.

    abstract:LEARNING OBJECTIVES:After reading this article, the participant should be able to: 1. Clinically describe various cutaneous neoplasms. 2. Identify the corresponding histopathologic findings. 3. Discuss the optimal treatment approach for each entity. SUMMARY:Cutaneous neoplasms are broadly viewed as benign, premalignan...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章,评审


    authors: Lee EH,Nehal KS,Disa JJ

    更新日期:2010-05-01 00:00:00

  • A Novel Model for Cutaneous Wound Healing and Scarring in the Rat.

    abstract:BACKGROUND:Current rodent models of wound healing and scarring are flawed because of rapid wound contraction and inconspicuous scarring after healing, which is not closely parallel to the physiologic process in humans. This study aimed to establish a novel model of wound healing and scarring in rats. METHODS:Excisiona...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Zhou S,Wang W,Zhou S,Zhang G,He J,Li Q

    更新日期:2019-02-01 00:00:00

  • Redefining the Ideal Buttocks: A Population Analysis.

    abstract:BACKGROUND:The buttocks are a key element of female beauty, with aesthetic gluteoplasty becoming one of the fastest growing plastic surgery procedures. However, there remains no clear standard for the ideal buttocks. The authors performed a population analysis of the characteristics of the ideal buttocks to guide surgi...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Wong WW,Motakef S,Lin Y,Gupta SC

    更新日期:2016-06-01 00:00:00

  • The radial forearm flap donor site: should we vein graft the artery? A comparative study.

    abstract::Controversy exists in the literature regarding reconstruction of the radial artery after elevation of the radial forearm flap. The literature suggests that reconstructing the radial artery with a vein graft is an important aspect in the use of this flap. In our experience, this has never been performed with no sequela...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Meland NB,Core GB,Hoverman VR

    更新日期:1993-04-01 00:00:00

  • Clinical and Quantitative Isokinetic Comparison of Abdominal Morbidity and Dynamics following DIEP versus Muscle-Sparing Free TRAM Flap Breast Reconstruction.

    abstract:BACKGROUND:The deep inferior epigastric perforator (DIEP) flap, which is a modification of the muscle-sparing free transverse rectus abdominis musculocutaneous (TRAM) flap, is being more frequently used in an effort to reduce postoperative abdominal morbidity. However, there is no consensus as to which of these flaps i...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Uda H,Kamochi H,Sarukawa S,Sunaga A,Sugawara Y,Yoshimura K

    更新日期:2017-12-01 00:00:00

  • The Pushback Pharyngeal Flap: An 18-Year Experience.

    abstract:BACKGROUND:The pharyngeal flap is one of the oldest and most popular techniques for correction of velopharyngeal insufficiency. The authors describe a large series using a technique that combines a pharyngeal flap with a palate pushback to avoid common causes of operative failure while restoring the velopharyngeal mech...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Rochlin DH,Mittermiller PA,Sheckter CC,Menard RM

    更新日期:2019-06-01 00:00:00

  • Health-related quality-of-life assessment and surgical outcomes for auricular reconstruction using autologous costal cartilage.

    abstract:BACKGROUND:This study aims to assess the health-related quality-of-life benefit following auricular reconstruction using autologous costal cartilage in children. In addition, key aspects of the surgical reconstruction are assessed. METHODS:After auricular reconstruction, patients completed two questionnaires. The firs...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Soukup B,Mashhadi SA,Bulstrode NW

    更新日期:2012-03-01 00:00:00

  • Anatomical study of the medial crura and the effect on nasal tip projection in open rhinoplasty.

    abstract:BACKGROUND:Common variations in morphology of the medial crura have been described. The authors' observation is that changes in nasal tip projection depend on the shape and strength of the medial crura. The authors investigated how differences in medial crura shape affect tip projection after surgical intervention. ME...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Patel KB,Mendonca DA,Skolnick G,Woo AS

    更新日期:2013-10-01 00:00:00

  • Keloids have continuous high metabolic activity.

    abstract::The adenosine triphosphate (ATP) content of keloids and scars resected from patients was demonstrated by high-performance liquid chromatography. The surface color of hypertrophic scars was red or pink and that of atrophic scars was white. The ATP content of red scars was (in mmol/g protein) 1.06 +/- 0.14, of pink scar...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Ueda K,Furuya E,Yasuda Y,Oba S,Tajima S

    更新日期:1999-09-01 00:00:00

  • The effect of age on human adipose-derived stem cells.

    abstract:BACKGROUND:Adipose-derived mesenchymal stem cells are a robust, multipotent cell source. They are easily harvested and exhibit promise in a variety of regenerative applications. The purpose of this study was to evaluate the aging impact on adipose-derived mesenchymal stem cells, relating to morphology, senescent proper...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Wu W,Niklason L,Steinbacher DM

    更新日期:2013-01-01 00:00:00

  • Quadrilateral space syndrome: diagnosis and operative decompression technique.

    abstract::We present a series of five patients with quadrilateral space syndrome. All patients had the diagnosis made on the basis of (1) tenderness over the quadrilateral space, (2) paresthesia over the lateral shoulder and upper posterior arm, and (3) deltoid weakness associated with decreased shoulder abduction. A history of...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Francel TJ,Dellon AL,Campbell JN

    更新日期:1991-05-01 00:00:00

  • Kinetics of E-selectin expression in surgical flaps.

    abstract::During the ischemia/reperfusion phenomenon, adhesion molecules seem to play a critical role in the recruitment of neutrophils to sites of eventual tissue injury. E-selectin is an endothelium-derived molecule that mediates adhesion of neutrophils to activated endothelial cells. In vitro expression of E-selectin, after ...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Wang C,Kerrigan CL,Stotland MA

    更新日期:1997-11-01 00:00:00

  • Complex abdominal-perineal reconstruction: options after pelvic bone resection.

    abstract::Reconstruction of abdominal-perineal wounds after tumor extirpation presents the plastic surgeon with a variety of complex reconstructive options. These options are complicated by the resection of the bony pelvis. Two cases are presented that combine the use of synthetic mesh for pelvic ring and floor reconstruction w...

    journal_title:Plastic and reconstructive surgery

    pub_type: 杂志文章


    authors: Evans GR,Goldberg DP,Ames FC

    更新日期:1996-09-01 00:00:00

  • Comparison of Limited and Full Dissection Abdominoplasty Using Laser Fluorescence Imaging to Evaluate Perfusion of the Abdominal Skin.

    abstract:BACKGROUND:In an effort to reduce risk, some surgeons promote a limited dissection abdominoplasty technique. They believe that preserving musculocutaneous perforators improves blood supply to the overlying abdominal tissue and that improved vascularity avoids seromas and reduces wound healing problems. Laser fluorescen...

    journal_title:Plastic and reconstructive surgery

    pub_type: 临床试验,杂志文章


    authors: Swanson E

    更新日期:2015-07-01 00:00:00