Abstract:
:Hippocampal mossy fiber axons simultaneously activate CA3 pyramidal cells and stratum lucidum interneurons (SLINs), the latter providing feedforward inhibition to control CA3 pyramidal cell excitability. Filopodial extensions of giant boutons of mossy fibers provide excitatory synaptic input to the SLIN. These filopodia undergo extraordinary structural plasticity causally linked to execution of memory tasks, leading us to seek the mechanisms by which activity regulates these synapses. High-frequency stimulation of the mossy fibers induces long-term depression (LTD) of their calcium-permeable AMPA receptor synapses with SLINs; previous work localized the site of induction to be postsynaptic and the site of expression to be presynaptic. Yet, the underlying signaling events and the identity of the retrograde signal are incompletely understood. We used whole cell recordings of SLINs in hippocampal slices from wild-type and mutant mice to explore the mechanisms. Genetic and pharmacologic perturbations revealed a requirement for both the receptor tyrosine kinase TrkB and its agonist, brain-derived neurotrophic factor (BDNF), for induction of LTD. Inclusion of inhibitors of Trk receptor kinase and PLC in the patch pipette prevented LTD. Endocannabinoid receptor antagonists and genetic deletion of the CB1 receptor prevented LTD. We propose a model whereby release of BDNF from mossy fiber filopodia activates TrkB and PLCγ1 signaling postsynaptically within SLINs, triggering synthesis and release of an endocannabinoid that serves as a retrograde signal, culminating in reduced glutamate release. Insights into the signaling pathways by which activity modifies function of these synapses will facilitate an understanding of their contribution to the local circuit and behavioral consequences of hippocampal granule cell activity. NEW & NOTEWORTHY We investigated signaling mechanisms underlying plasticity of the hippocampal mossy fiber filopodial synapse with interneurons in stratum lucidum. High-frequency stimulation of the mossy fibers induces long-term depression of this synapse. Our findings are consistent with a model in which brain-derived neurotrophic factor released from filopodia activates TrkB of a stratum lucidum interneuron; the ensuing activation of PLCγ1 induces synthesis of an endocannabinoid, which provides a retrograde signal leading to reduced release of glutamate presynaptically.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Pan E,Zhao Z,McNamara JOdoi
10.1152/jn.00669.2018subject
Has Abstractpub_date
2019-02-01 00:00:00pages
609-619issue
2eissn
0022-3077issn
1522-1598journal_volume
121pub_type
杂志文章abstract::1. High-threshold Ca2+ currents were studied in large pyramidal cells acutely dissociated from the rat amygdaloid complex. L-, N-, and P-type currents were present in about equal proportions in these cells and accounted for most of the whole-cell current. ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.71.1.433
更新日期:1994-01-01 00:00:00
abstract::Our objective was to assess the mechanical changes associated with spasticity in elbow muscles of chronic hemiparetic stroke survivors and to compare these changes with those recorded in the ankle muscles of a similar cohort. We first characterized elbow dynamic stiffness by applying pseudorandom binary positional per...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00049.2007
更新日期:2007-08-01 00:00:00
abstract::1. The individual joint rotations of a movement sequence might be controlled either by a central motor plan or by motion-dependent (i.e., kinesthetic) sensory input. Most previous research has focused on how the nervous system uses central motor plans to control movement sequences. This study examined how the nervous ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.63.1.161
更新日期:1990-01-01 00:00:00
abstract::To determine whether visual discrimination in macaque frontal eye field (FEF) is contingent on saccade planning, unit activity was recorded in two monkeys during blocked go and no-go visual search trials. The eye movements made by monkeys after correct no-go trials, in addition to an attenuation of the visual response...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.2.1046
更新日期:1997-02-01 00:00:00
abstract::An orthodromically evoked late hyperpolarizing potential (LHP) was studied using intracellular recording techniques in rat hippocampal CA1 pyramidal cells in vitro. Several tests indicated that the LHP is not blocked by GABA antagonists, but rather comprises the initial portion of the stimulation-induced burst afterhy...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.52.5.892
更新日期:1984-11-01 00:00:00
abstract::Following peripheral nerve injury, moderate daily exercise conducted on a level treadmill results in enhanced axon regeneration and modest improvements in functional recovery. If the exercise is conducted on an upwardly inclined treadmill, even more motor axons regenerate successfully and reinnervate muscle targets. W...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00129.2016
更新日期:2016-09-01 00:00:00
abstract::1. Previous studies indicate that after transection of the rostral spinal cord, larval lamprey begin to recover locomotor behavior 2 wk posttransection and recovery is complete at approximately 8 wk. To examine the mechanisms underlying behavioral recovery after spinal cord transection, in the present study the time c...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.72.2.847
更新日期:1994-08-01 00:00:00
abstract::During early development neuronal networks express slow oscillating synchronized activity. The activity can be driven by several, not necessarily mutually exclusive, mechanisms. Each mechanism might have distinctive consequences for the phenomenology, formation, or sustainment of the early activity pattern. Here we st...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00889.2010
更新日期:2011-09-01 00:00:00
abstract::Neural precursor cells (NPCs) in the mammalian olfactory bulb give rise to local inhibitory neurons that integrate into existing circuitry throughout adult life. However, the functional properties of neurotransmitter receptors expressed by NPCs are not well understood. In this study, we use patch-clamp recording and c...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00821.2009
更新日期:2010-03-01 00:00:00
abstract::1. We investigated the glycine-induced response in ventromedial hypothalamic (VMH) neurons freshly dissociated from 8- to 12-day-old rats using the nystatin and gramicidin perforated patch recording modes. The nystatin-formed pores in the plasma membrane are permeable for both monovalent cations and anions, whereas th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1994.72.4.1530
更新日期:1994-10-01 00:00:00
abstract::Odor responses of neocortical neurons in rabbits under light pentobarbital anesthesia were studied in a restricted area in the lateral half of the prefrontal cortex close to the frontal pole. A preevent cumulative sum chart provided a visual display of the change in unit discharge rate following each odor stimulation,...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.52.4.638
更新日期:1984-10-01 00:00:00
abstract::How the CNS masters the many degrees of freedom of the musculoskeletal system to control goal-directed movements is a long-standing question. We have recently provided support to the hypothesis that the CNS relies on a modular control architecture by showing that the phasic muscle patterns for fast reaching movements ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01377.2007
更新日期:2008-09-01 00:00:00
abstract::Brief movements of a large-field visual stimulus elicit short-latency tracking eye movements termed "ocular following responses" (OFRs). To address the question of whether OFRs can be elicited by purely binocular motion signals in the absence of monocular motion cues, we measured OFRs from monkeys using dichoptic moti...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01316.2007
更新日期:2008-05-01 00:00:00
abstract::1. Bicuculline-induced epileptiform bursts in slices of neocortical tissue resected from children (3 mo to 14 yr) undergoing neurosurgical treatment for intractable epilepsy were studied with conventional intracellular recording techniques. The purposes of this study were to characterize the bursts evoked in immature ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1993.70.5.1759
更新日期:1993-11-01 00:00:00
abstract::In rats, whisker trimming during development leads to persistent alterations in the function of cortical barrel circuits and to behavioral deficits later in life. Here we examined how whisker trimming begun either at birth (P0) or on postnatal day 12 (P12), around the onset of whisking behavior, affects receptive fiel...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00569.2005
更新日期:2005-12-01 00:00:00
abstract::1. The purpose of this study is to analyze the kinematics of stepping from the point of view of limb control by attempting to dissociate the various neuronal and mechanical factors that shape actual movements observed experimentally. The cat hindlimb, like the primate arm, is a multijointed mechanical system with redu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.74.6.2266
更新日期:1995-12-01 00:00:00
abstract::Contrasting theories of visual attention have emphasized selection by spatial location, individual features, and whole objects. We used functional magnetic resonance imaging to ask whether and how attention to one feature of an object spreads to other features of the same object. Subjects viewed two spatially superimp...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00828.2012
更新日期:2013-11-01 00:00:00
abstract::1. Retzius (R) neurons of the fifth and sixth segmental ganglia of the leech, called R(5,6) neurons are specialized to innervate the adjacent reproductive organs and are morphologically and functionally distinct from R neurons of standard ganglia [R(x) cells]. In this study the electrical properties of the R(x) and R(...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1995.73.3.957
更新日期:1995-03-01 00:00:00
abstract::Spatiotemporal patterns of dorsal root-evoked potentials were studied in transverse slices of the rat spinal cord by monitoring optical signals from a voltage-sensitive dye with multiple-photodiode optic camera. Typically, dorsal root stimulation generated two basic waveforms of voltage images: dual-component images c...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00209.2005
更新日期:2005-09-01 00:00:00
abstract::Trimming all but two whiskers in adult rats produces a predictable change in cortical cell-evoked responses characterized by increased responsiveness to the two intact whiskers and decreased responsiveness to the trimmed whiskers. This type of synaptic plasticity in rat somatic sensory cortex, called "whisker pairing ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.6.3216
更新日期:1998-06-01 00:00:00
abstract::Although fluorescence microscopy has proven to be one of the most powerful tools in biology, its application to the intact animal has been limited to imaging several hundred micrometers below the surface. The rest of the animal has eluded investigation at the microscopic level without excising tissue or performing ext...
journal_title:Journal of neurophysiology
pub_type:
doi:10.1152/jn.01007.2003
更新日期:2004-04-01 00:00:00
abstract::Cardiovascular function is regulated by a dynamic balance composed of sympathetic and parasympathetic activity. Sympathoexcitatory presympathetic neurons (PSNs) in the rostral ventrolateral medulla project directly to cardiac and vasomotor sympathetic preganglionic neurons in the spinal cord. In proximity to the PSNs ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01054.2012
更新日期:2013-08-01 00:00:00
abstract::The deep dorsal horn represents a major site for the integration of spinal sensory information. The bulbospinal monoamine transmitters, released from serotonergic, noradrenergic, and dopaminergic systems, exert modulatory control over spinal sensory systems as does acetylcholine, an intrinsic spinal cord biogenic amin...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.86.5.2183
更新日期:2001-11-01 00:00:00
abstract::Effective connectivity between cortical areas decreases during slow wave sleep. This decline can be observed in the reduced interareal propagation of activity evoked either directly in cortex by transcranial magnetic stimulation (TMS) or by sensory stimulation. We present here a large-scale model of the thalamocortica...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00059.2009
更新日期:2009-10-01 00:00:00
abstract::Impulse from soleus muscle afferents were recorded in premammillary cats that were walking on a treadmill. In normal walking the effects of gamma-motoneurons on impulse rates of muscle spindle afferents are confounded by the effects of the large length changes that occur. To isolate the effects of gamma-motoneurons th...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1985.53.2.341
更新日期:1985-02-01 00:00:00
abstract::Sciatic nerve section (axotomy) increases the excitability of rat dorsal root ganglion (DRG) neurons. The changes in Ca2+ currents, K+ currents, Ca2+ sensitive K+ current, and hyperpolarization-activated cation current (I(H)) that may be associated with this effect were examined by whole cell recording. Axotomy affect...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.85.2.644
更新日期:2001-02-01 00:00:00
abstract::The Wilson-Cowan equations were originally shown to produce limit cycle oscillations for a range of parameters. Others subsequently showed that two coupled Wilson-Cowan oscillators could produce chaos, especially if the oscillator coupling was from inhibitory interneurons of one oscillator to excitatory neurons of the...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00323.2019
更新日期:2019-12-01 00:00:00
abstract::Studying adaptation to Coriolis perturbations of arm movements has advanced our understanding of motor control and learning. We have now applied this paradigm to two-dimensional postural sway. We measured how subjects (n = 8) standing at the center of a fully enclosed rotating room who made voluntary anterior-posterio...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00606.2018
更新日期:2019-06-01 00:00:00
abstract::1. We recorded single cell activities in the caudate nucleus of the monkeys trained to perform a series of visuomotor tasks. In the first part of this paper, we summarize the types and locations of neurons in the monkey caudate nucleus. In the second part, we report the characteristics of neurons related to saccadic e...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1989.61.4.780
更新日期:1989-04-01 00:00:00
abstract::The cholinergic system is critically involved in synaptic models of learning and memory by enhancing dendritic [Ca(2+)](i) signals. Diffuse cholinergic innervation suggests subcellular modulation of membrane currents and Ca(2+) signals. Here we use ion-selective microelectrodes to study spread of carbachol (CCh) after...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1999.82.4.1909
更新日期:1999-10-01 00:00:00