Upregulation of autophagy by Ginsenoside Rg2 in MCF-7 cells.


:Autophagy is a major intracellular degradation process that plays an important role in cell survival, stress responses, nutrient sensing and development. Our previous studies have shown that Rg2, a triterpenoid saponin contained in ginseng, protects cells against UVB-induced genotoxicity by increasing DNA repair, in possible association with modulation of protein levels involved in p53 pathway. In this study, we determined an upregulation of autophagy by Rg2. Rg2 treatment for 24 h in MCF-7, a breast cancer cell, did not show cytotoxicity up to 200 μM. Rg2 also upregulated the level of p-p53, p-AMPK, p-ACC, Atg-7 and LC3-II and decreased the level of p62 in concentration-dependent manners. We also determined the level of p53, AMPK, p62, Atg-7 and LC3 after UVB exposure and subsequent incubation in growth medium for 24 h. UVB increased the level of p-p53, p-AMPK, p-ACC and decreased the levels of p62, Atg-7 and LC3-II. Interestingly, Rg2 treatment for 24 h after UVB exposure increased the levels of p-p53, p-AMPK, p-ACC, Atg-7 and LC3-II and decreased the level of cyclobutane pyrimidine dimer, a UVB-induced DNA damage in concentration-dependent manners. All these results suggest that Rg2 increased autophagy and decreased UVB-induced DNA damage, in possible association with the modulation of protein levels in p53- and autophagic pathways.


Animal cells and systems


Chung Y,Jeong S,Choi HS,Ro S,Lee JS,Park JK




Has Abstract


2018-11-19 00:00:00














  • Vascular endothelial growth factor improves the therapeutic effects of cyclodextrin in Niemann-Pick type C mice.

    abstract::Niemann-Pick type C disease (NP-C) is a fatal neurodegenerative disorder caused by a deficiency in the function of the NPC1 gene. Malfunction of this gene/protein leads to progressive accumulation of unesterified cholesterol and sphingolipids in many organs, including the brain. To date, drugs that target pivotal stag...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Jeong MS,Bae JS,Jin HK

    更新日期:2019-08-25 00:00:00

  • Predicting climate-driven shifts in the breeding phenology of Varied Tits (Sittiparus various) in South Korean forests.

    abstract::Phenological shifts of plants and animals due to climate change can vary among regions and species, requiring study of local ecosystems to understand specific impacts. The reproductive timing of insectivorous songbirds in temperate forests is tightly synchronized with peak prey abundance, and thus they can be suscepti...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Jeong MS,Choi CY,Kim H,Lee WS

    更新日期:2019-10-10 00:00:00

  • Microstructure and mechanical properties of rostrum in Cyrtotrachelus longimanus (Coleoptera: Curculionidae).

    abstract::The microstructure, composition and mechanical properties of the rostrum in Cyrtotrachelus longimanus (JHC Fabre) were studied utilizing light, fluorescent, scanning electron microscopy (SEM) and energy-dispersive spectroscopy. SEM images show the morphological characteristics of rostrum's cross section; it is a typic...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Li L,Guo C,Li X,Xu S,Han C

    更新日期:2017-05-31 00:00:00

  • Localization of integrin heterodimer α9β1 on the surface of uterine endometrial stromal and epithelial cells in mice.

    abstract::Previously, we reported that endometrial stromal (ES) and endometrial epithelial (EE) cells did not attach to tenascin C, indicating the absence of active integrin α9β1 on the surface of mouse ES and EE cells. However, that study used recombinant tenascin C without fibronectin (FN) type III repeats interacting with in...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Park HJ,Yun JI,Lee ST

    更新日期:2020-08-05 00:00:00

  • A new southern distribution limit for Sorex mirabilis Ognev, 1937 (Eulipotyphla, Soricidae).

    abstract::Sorex mirabilis, a small insectivorous mammal, is a very rare, poorly known species distributed at high latitudes and altitudes of northeastern Asia. The occurrence of this species in the Republic of Korea was known from late 1990s. We found a new specimen from a location of a 50' lower latitude and 400 m lower altitu...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Park SJ,Lee WS

    更新日期:2019-01-03 00:00:00

  • Distribution status and habitat characteristics of the endangered freshwater fish, Microphysogobio rapidus (Cyprinidae).

    abstract::To investigate distribution, habitat characteristics, and current conservation status of the endangered endemic species, rapid small gudgeon Microphysogobio rapidus (Cyprinidae), we surveyed a total of 79 sites from the historic records (20 sites) plus additional sites (59 sites) with good habitat conditions, analyzed...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Hong YK,Sung HC,Ko MH,Kim KS,Bang IC

    更新日期:2017-07-10 00:00:00

  • Knockdown of LncRNAZFAS1 suppresses cell proliferation and metastasis in non-small cell lung cancer.

    abstract::To evaluate the effects of LncRNAZFAS1 on cell proliferation and tumor metastasis in non-small cell lung cancer (NSCLC), we detected the expression level of LncRNAZFAS1 in NSCLC-related tissues and cells. qRT-PCR results revealed that LncRNAZFAS1 in tumor tissues was significantly higher than that in normal lung tissu...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Zhou Y,Hu XW,Yang SJ,Yu Z

    更新日期:2020-03-02 00:00:00

  • Metagenomic analysis of the marine coastal invertebrates of South Korea as assessed by Ilumina MiSeq.

    abstract::Metagenomic analysis was carried out for the first time on the marine coastal invertebrates of South Korea. Samples collected from coastal areas of Korea were filtered through a 63 µm mesh, then their 18S rDNA V4 regions were amplified. High-throughput sequencing of PCR amplicons using Illumina MiSeq and BLAST against...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Kim H,Kim H,Hwang HS,Kim W

    更新日期:2016-12-30 00:00:00

  • Optimization of episomal reprogramming for generation of human induced pluripotent stem cells from fibroblasts.

    abstract::Generation of induced pluripotent stem cells (iPSCs) by defined factors (OCT4, SOX2, C-MYC, and KLF4) from various human primary cells has been reported. Human fibroblasts have been widely used as a cellular source in reprogramming studies over recent decades. The original method of iPSC generation uses retro- or lent...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Bang JS,Choi NY,Lee M,Ko K,Lee HJ,Park YS,Jeong D,Chung HM,Ko K

    更新日期:2018-03-15 00:00:00

  • Upregulated lncRNA Gm2044 inhibits male germ cell development by acting as miR-202 host gene.

    abstract::Long non-coding RNAs (lncRNAs) have been found to participate in the regulation of human spermatogenic cell development. However, little is known about the abnormal expression of lncRNAs associated with spermatogenic failure and their molecular mechanisms. Using lncRNA microarray of testicular tissue for male infertil...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Liang M,Hu K,He C,Zhou J,Liao Y

    更新日期:2019-03-14 00:00:00

  • Exploring glia to better understand Alzheimer's disease.

    abstract::The amyloid-β (Aβ) hypothesis has been the leading explanation for the pathogenesis of Alzheimer's disease (AD). The most common traits of AD are cognitive impairments and memory loss, which are associated with the accumulation of Aβ. Aβ aggregates activate glial cells, which in turn remove Aβ. Because microglia act a...

    journal_title:Animal cells and systems

    pub_type: 杂志文章,评审


    authors: Kim YS,Jung HM,Yoon BE

    更新日期:2018-08-12 00:00:00

  • Upregulating miR-637 aggravates endoplasmic reticulum stress-induced apoptosis in gastric cancer cells by suppressing Calreticulin.

    abstract::Gastric cancer is a leading cause of cancer death worldwide. Endoplasmic reticulum (ER) stress-induced apoptosis has been confirmed to be important in the treatment of gastric cancer. MiR-637 has recently been found to exert inhibitory effects on gastric cancer, and this study aimed to investigate whether miR-637 coul...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Kong Q,Zhang Z,Liang Z

    更新日期:2020-09-10 00:00:00

  • Dusp1 modulates activin/smad2 mediated germ layer specification via FGF signal inhibition in Xenopus embryos.

    abstract::Activin, a member of the transforming growth factor (TGF-β) superfamily, induces mesoderm, endoderm and neuro-ectoderm formation in Xenopus embryos. Despite several previous studies, the complicated gene regulatory network and genes involved in this induction await more elaboration. We identified expression of various...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Umair Z,Kumar S,Rafiq K,Kumar V,Reman ZU,Lee SH,Kim S,Lee JY,Lee U,Kim J

    更新日期:2020-11-27 00:00:00

  • Paternal bias expression of Igf2as is enhancer-dependent on the imprinting cluster of Igf2, H19 and Nctc1 in muscle cells.

    abstract::Igf2, H19, and Nctc1 are linked co-regulated genes on distal mouse chromosome 7. This locus is an important model both for studying mechanisms of monoallelic expression and for elucidating the role of cis-regulatory elements - enhancers and insulators - in organizing chromatin and gene expression across a large domain...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Kim JY,Youn HY,Pfeifer K,Eun B

    更新日期:2019-05-16 00:00:00

  • Endothelin-converting enzyme-1 expression in acute and chronic liver injury in fibrogenesis.

    abstract::Endothelin-1 (ET-1) induces contraction, proliferation, and collagen synthesis of activated hepatic stellate cells and is a potent mediator of portal hypertension. Endothelin-converting enzyme-1 (ECE-1) generates ET-1 from the inactive precursor big-endothelin-1. The cellular distribution and activity of ECE-1 in the ...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Cho TJ,Kim HJ,Cho J

    更新日期:2019-06-09 00:00:00

  • How to interpret and integrate multi-omics data at systems level.

    abstract::Current parallel sequencing technologies generate biological sequence data explosively and enable omics studies that analyze collective biological features. The more omics data that is accumulated, the more they show the regulatory complexity of biological phenotypes. This high order regulatory complexity needs system...

    journal_title:Animal cells and systems

    pub_type: 杂志文章,评审


    authors: Jung GT,Kim KP,Kim K

    更新日期:2020-01-30 00:00:00

  • Betaine enhances the cellular survival via mitochondrial fusion and fission factors, MFN2 and DRP1.

    abstract::Betaine is a key metabolite of the methionine cycle and known for attenuating alcoholic steatosis in the liver. Recent studies have focused on the protection effect of betaine in mitochondrial regulation through the enhanced oxidative phosphorylation system. However, the mechanisms of its beneficial effects have not b...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Jung Kim M

    更新日期:2018-08-30 00:00:00

  • Transcriptome sequencing combined with bioinformatics predicts potential genes and pathways associated with bupivacaine-induced apoptosis.

    abstract::This study aimed to explore the potential genes and pathways associated with bupivacaine-induced apoptosis. Human neuroblastoma cell line SH-SY5Y was used in this study. The effect of bupivacaine on cell viability of SH-SY5Y was detected by Cell Counting Kit-8. Transcriptome sequencing was performed for SH-SY5Y cells ...

    journal_title:Animal cells and systems

    pub_type: 杂志文章


    authors: Wei P,Wang T,Han S,Ma G,Gao S,Yang Z

    更新日期:2017-02-22 00:00:00