miR-627/HMGB1/NF-κB regulatory loop modulates TGF-β1-induced pulmonary fibrosis.


:Pulmonary fibrosis (PF) is a fibroproliferative disease that can eventually lead to fatal lung failure. It is characterized by abnormal proliferation of fibroblasts, dysregulated fibroblast differentiation to myofibroblast, and disorganized collagen and extracellular matrix production, deposition and degradation. There is still a lack of effective treatment strategies for PF. Extracellular high-mobility group box protein 1 (HMGB1) induces PF through NF-κB-mediated TGF-β1 release. Herein, we first validate the suppressive effect of HMGB1 knockdown on TGF-β1-induced α-smooth muscle actin (α-SMA) and collagen I protein expression. In PF, miRNAs exert different effects through targeting various downstream target messenger RNAs. We searched an online database for dysregulated miRNAs in PF tissues; among them, miR-627 was predicted by online tools to target HMGB1 to inhibit its expression. miR-627 overexpression could partially reverse TGF-β1-induced normal human lung fibroblast proliferation, as well as α-SMA and collagen I protein expression. miR-627 inhibition could partially reverse the suppressive effect of HMGB1 knockdown on TGF-β1-induced α-SMA and collagen I protein expression through direct binding to the 3'-untranslated region of HMGB1. Moreover, miR-627/HMGB1 affected TGF-β1 release through RAGE/NF-κB signaling; miR-627/HMGB1 and RAGE/NF-κB signaling formed a regulatory loop to modulate TGF-β1-induced PF in vitro. In conclusion, miR-627 may be a potential agent that targets HMGB1 to inhibit its expression, thereby improving TGF-β1-induced PF in vitro.


J Cell Biochem


Li J,Kong X,Jiang S,Liao W,Zhang Z,Song J,Liang Y,Zhang W




Has Abstract


2019-03-01 00:00:00












  • High expression of SGTA in esophageal squamous cell carcinoma correlates with proliferation and poor prognosis.

    abstract::Receptor tyrosine kinases (RTKs) expression and the growth factor such as platelet-derived growth factor (PDGF) and their receptors have been considered relevant in the process of angiogenesis and dissemination in esophageal squamous cell carcinoma (ESCC). Small glutamine-rich tetratricopeptide repeat-containing prote...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yang X,Cheng L,Li M,Shi H,Ren H,Ding Z,Liu F,Wang Y,Cheng C

    更新日期:2014-01-01 00:00:00

  • Identification of an IQGAP1/AKAP79 complex in beta-cells.

    abstract::IQGAP1, is a recently discovered scaffold protein proposed to regulate membrane cytoskeleton events through protein-protein interactions with F-actin, E-cadherin, beta-catenin, and CLIP170. The binding of IQGAP1 to its partners is regulated by calcium/calmodulin (Ca(++)/CaM) and the small molecular weight guanine nucl...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Nauert JB,Rigas JD,Lester LB

    更新日期:2003-09-01 00:00:00

  • Characterization of LipN (Rv2970c) of Mycobacterium Tuberculosis H37Rv and its Probable Role in Xenobiotic Degradation.

    abstract::LipN (Rv2970c) belongs to the Lip family of M. tuberculosis H37Rv and is homologous to the human Hormone Sensitive Lipase. The enzyme demonstrated preference for short carbon chain substrates with optimal activity at 45°C/pH 8.0 and stability between pH 6.0-9.0. The specific activity of the enzyme was 217 U/mg protein...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Jadeja D,Dogra N,Arya S,Singh G,Singh G,Kaur J

    更新日期:2016-02-01 00:00:00

  • Identification of messenger and long noncoding RNAs associated with gallbladder cancer via gene expression profile analysis.

    abstract::The present study aimed to investigate the long noncoding RNAs (lncRNAs) and messenger RNAs (mRNAs) involved in the progression of gallbladder cancer and explore the potential physiopathologic mechanisms of gallbladder cancer in terms of competing endogenous RNAs (ceRNAs). The original lncRNA and mRNA expression profi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Kong L,Wu Q,Zhao L,Ye J,Li N,Yang H

    更新日期:2019-12-01 00:00:00

  • The determination of the potential anticancer effects of Coriandrum sativum in PC-3 and LNCaP prostate cancer cell lines.

    abstract::Coriander (Coriandrum sativum L.) is such an herb from the Apiaceae family, used both for its medicinal and nutritional properties for many centuries. In this study, the effects of C. sativum extract on gene expression, viability, colony formation, migration, and invasion of PC-3 and LNCaP prostate cancer cell lines h...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Elmas L,Secme M,Mammadov R,Fahrioglu U,Dodurga Y

    更新日期:2019-03-01 00:00:00

  • Oxysterol-induced osteogenic differentiation of marrow stromal cells is regulated by Dkk-1 inhibitable and PI3-kinase mediated signaling.

    abstract::Osteoporosis and its complications cause morbidity and mortality in the aging population, and result from increased bone resorption by osteoclasts in parallel with decreased bone formation by osteoblasts. A widely accepted strategy for improving bone health is targeting osteoprogenitor cells in order to stimulate thei...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Amantea CM,Kim WK,Meliton V,Tetradis S,Parhami F

    更新日期:2008-10-01 00:00:00

  • Characterization of caveolae from rat heart: localization of postreceptor signal transduction molecules and their rearrangement after norepinephrine stimulation.

    abstract::Caveolae are plasma membrane subcompartments that have been implicated in signal transduction. In many cellular systems, caveolae are rich in signal transduction molecules such as G proteins and receptor-associated tyrosine kinases. An important structural component of the caveolae is caveolin. Recent evidence show th...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: De Luca A,Sargiacomo M,Puca A,Sgaramella G,De Paolis P,Frati G,Morisco C,Trimarco B,Volpe M,Condorelli G

    更新日期:2000-04-01 00:00:00

  • Acute physical exercise increases leptin-induced hypothalamic extracellular signal-regulated kinase1/2 phosphorylation and thermogenesis of obese mice.

    abstract::The obesity is a result of energy imbalance and the increase in thermogenesis seems an interesting alternative for the treatment of this disease. The mechanism of energy expenditure through thermogenesis is tightly articulated in the hypothalamus by leptin. The hypothalamic extracellular signal-regulated kinase-1/2 (E...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Gaspar RC,Muñoz VR,Kuga GK,Nakandakari SCBR,Minuzzi LG,Botezelli JD,da Silva ASR,Cintra DE,de Moura LP,Ropelle ER,Pauli JR

    更新日期:2019-01-01 00:00:00

  • Molybdenum enzymes and molybdenum cofactor in mycobacteria.

    abstract::When intracelluar pathogens enter the host macrophages where in addition to oxidative and antibiotic mechanisms of antimicrobial activity, nutrients are deprived. Human pathogen Mycobacterium tuberculosis is one of macrophage parasitisms, which can replicate and persist for decades in dormancy state in virulent enviro...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Shi T,Xie J

    更新日期:2011-10-01 00:00:00

  • Study on the relationship between insulin growth factor 1 and liver fibrosis in patients with chronic hepatitis C with type 2 diabetes mellitus.

    abstract:OBJECTIVE:To investigate the correlation between serum protein level of insulin growth factor 1 (IGF-1) and the degree of liver fibrosis in patients with chronic hepatitis C (CHC) combined with type 2 diabetes mellitus (T2DM). METHODS:The cases are divided into four groups. Then serum levels of IFG-1, alanine aminotra...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Cao LH,Lu FM,Lu XJ,Zhu LY

    更新日期:2018-11-01 00:00:00

  • Inhibition of erythrocyte membrane shape change by band 3 cytoplasmic fragment.

    abstract::The ATP-dependent transformation of crenated white human erythrocyte ghosts into smoothed disc and cup forms is inhibited by the soluble 40-45-kilodalton (kDa) cytoplasmic portion of the major transmembrane protein, band 3. The band 3 fragment was prepared by chymotryptic treatment of inverted vesicles stripped of per...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Carter DP,Fairbanks G

    更新日期:1984-01-01 00:00:00

  • A phenolic acid phenethyl urea derivative protects against irradiation-induced osteoblast damage by modulating intracellular redox state.

    abstract::Because irradiation may cause osteoradionecrosis, antioxidant supplementation is often used to suppress irradiation-mediated injury. This study examined whether a synthetic phenethyl urea compound, (E)-1-(3,4-dihydroxyphenethyl)-3-(3,4-dihydroxystyryl)urea (DPDS-U), prevents irradiation-mediated cellular damage in MC3...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Kim KA,Kook SH,Song JH,Lee JC

    更新日期:2014-11-01 00:00:00

  • Functional domains of necdin for protein-protein interaction, nuclear matrix targeting, and cell growth suppression.

    abstract::Necdin is a growth suppressor expressed predominantly in postmitotic neurons. The necdin gene is involved in the etiology of the genomic imprinting-associated neurodevelopmental disorder Prader-Willi syndrome and belongs to the MAGE gene family. All the MAGE family proteins contain a large homology domain termed the M...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Taniura H,Kobayashi M,Yoshikawa K

    更新日期:2005-03-01 00:00:00

  • Embryonic undifferentiated cells show scattering activity on a surface coated with immobilized E-cadherin.

    abstract::Rearrangement of cell-cell adhesion is a critical event in embryonic development and tissue formation. We investigated the regulatory function of E-cadherin, a key adhesion protein, in the developmental process by using E-cadherin/IgG Fc fusion protein as an adhesion matrix in cell culture. F9 embryonal carcinoma cell...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Nagaoka M,Ise H,Harada I,Koshimizu U,Maruyama A,Akaike T

    更新日期:2008-01-01 00:00:00

  • Methylation of progesterone receptor isoform A promoter in normal breast tissue: An epigenetic link between early age at menarche and risk of breast cancer?

    abstract::Emerging evidence indicates that some altered patterns of methylation that occur in breast tumors may also be found in breast tissue of healthy women in relation to the breast cancer (BC) risk factors. Progesterone receptor (PR) isoform α is a crucial regulator of breast hormone responsiveness and its hypermethylation...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Daraei A,Izadi P,Khorasani G,Nafissi N,Naghizadeh MM,Younosi N,Meysamie A,Mansoori Y,Nariman-Saleh-Fam Z,Bastami M,Saadatian Z,Zendehbad Z,Tavakkoly-Bazzaz J

    更新日期:2019-08-01 00:00:00

  • Passage-dependent expression of STRO-1 in human gingival mesenchymal stem cells.

    abstract::The expression of STRO-1, the essential mesenchymal stem cell marker, was found to decrease with advancing passages in few tissues. Because STRO-1 was identified and isolated from human gingiva, we were interested to know its status after a few passages. Human gingival mesenchymal stem cells (HGMSCs) were isolated fro...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Ranga Rao S,Subbarayan R

    更新日期:2019-03-01 00:00:00

  • Gene coexpression networks analysis of sickle stroke risk.

    abstract::Stroke is one of the most destructive complications of sickle cell disease (SCD), and SCD is also the most common cause of childhood stroke. Sickle cell stroke is complex and has a genetic endothelial basis. Here, we further investigated this genetic basis using weighted gene coexpression network analysis. This system...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Liu FF,Wang J,Hu F,Wei Q,Li K

    更新日期:2019-09-01 00:00:00

  • Overexpression of regucalcin suppresses cell response for tumor necrosis factor-alpha or transforming growth factor-beta1 in cloned normal rat kidney proximal tubular epithelial NRK52E cells.

    abstract::The regulatory role of regucalcin on cell responses for tumor necrosis factor-alpha (TNF-alpha) or transforming growth factor-beta1 (TGF-beta1) was investigated using the cloned normal rat kidney proximal tubular epithelial NRK52E cells overexpressing regucalcin. NRK52E cells (wild type) and stable regucalcin (RC)/pCX...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Nakagawa T,Yamaguchi M

    更新日期:2007-04-01 00:00:00

  • miR-125b regulates side population in breast cancer and confers a chemoresistant phenotype.

    abstract::Resistance to chemotherapy is a major obstacle for the effective treatment of breast cancer and is partially due to the presence of drug resistant stem cell-like side population (SP). Previous studies have shown elevated miR-125b is associated with chemoresistance and metastasis; however, the relationship between miR-...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wang HJ,Guo YQ,Tan G,Dong L,Cheng L,Li KJ,Wang ZY,Luo HF

    更新日期:2013-10-01 00:00:00

  • Cultured AIDS-related Kaposi's sarcoma (AIDS-KS) cells demonstrate impaired bioenergetic adaptation to oxidant challenge: implication for oxidant stress in AIDS-KS pathogenesis.

    abstract::Despite its recognition as the most prevalent HIV associated cancer, speculation still abounds regarding the pathogenesis of AIDS-related Kaposi's sarcoma (AIDS-KS). However, it has been established that both cytokines, e.g. IL-6, and HIV-associated products, e.g., Tat, are integral in AIDS-KS cellular proliferation. ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Mallery SR,Bailer RT,Hohl CM,Ng-Bautista CL,Ness GM,Livingston BE,Hout BL,Stephens RE,Brierley GP

    更新日期:1995-11-01 00:00:00

  • Functional interrelationships between nuclear structure and transcriptional control: contributions to regulation of cell cycle- and tissue-specific gene expression.

    abstract::Multiple levels of nuclear structure contribute to functional interrelationships with transcriptional control in vivo. The linear organization of gene regulatory sequences is necessary but insufficient to accommodate the requirements for physiological responsiveness to homeostatic, developmental, and tissue-related si...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Stein GS,Stein JL,Lian JB,van Wijnen AJ,Montecino M

    更新日期:1996-08-01 00:00:00

  • Characterization of the nuclear matrix proteins in a transgenic mouse model for prostate cancer.

    abstract::The nuclear matrix (NM) contains a number of proteins that have been found to be associated with transformation. We have previously identified changes in the NM associated with prostate cancer. In this study, we examine the molecular changes that are associated with prostate cancer development in transgenic adenocarci...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Leman ES,Arlotti JA,Dhir R,Greenberg N,Getzenberg RH

    更新日期:2002-01-01 00:00:00

  • Simultaneous contraction and buckling of stress fibers in individual cells.

    abstract::It has been proposed that buckling of actin stress fibers (SFs) may be associated with their disassembly. However, much of the detail remains unknown partly because the use of an elastic membrane sheet, conventionally necessary for inducing SF buckling with a mechanical compression to adherent cells, may limit high qu...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Deguchi S,Matsui TS,Sato M

    更新日期:2012-03-01 00:00:00

  • Dietary Saturated Fat Promotes Development of Hepatic Inflammation Through Toll-Like Receptor 4 in Mice.

    abstract::Nonalcoholic steatohepatitis (NASH) is currently the third most common cause of end stage liver disease necessitating transplantation. The question remains how inflammation and NASH develop in the setting of nonalcoholic fatty liver disease (NAFLD) and steatosis. Understand the roles of toll-like receptor 4 (TLR4) and...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sutter AG,Palanisamy AP,Lench JH,Esckilsen S,Geng T,Lewin DN,Cowart LA,Chavin KD

    更新日期:2016-07-01 00:00:00

  • Isolation and characterization of squamous carcinoma cells resistant to photodynamic therapy.

    abstract::Photodynamic therapy (PDT) employing methyl δ-aminolevulinic acid (Me-ALA), as a precursor of the photosensitizer protoporphyrin IX (PpIX), is used for the treatment of non melanoma cutaneous cancer (NMCC). However, one of the problems of PDT is the apparition of resistant cell populations. The aim of this study was t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Milla LN,Cogno IS,Rodríguez ME,Sanz-Rodríguez F,Zamarrón A,Gilaberte Y,Carrasco E,Rivarola VA,Juarranz A

    更新日期:2011-09-01 00:00:00

  • LncRNA MALAT1 promotes oxidized low-density lipoprotein-induced autophagy in HUVECs by inhibiting the PI3K/AKT pathway.

    abstract::Emerging evidence suggests that long noncoding RNAs (lncRNAs) are involved in many biological processes, such as cell growth, differentiation, apoptosis, and autophagy. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), highly expressed in endothelial cells, is well conserved and implicated in endothelia...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Li S,Pan X,Yang S,Ma A,Yin S,Dong Y,Pei H,Bi X,Li W

    更新日期:2019-03-01 00:00:00

  • Loss of BMI-1 dampens migration and EMT of colorectal cancer in inflammatory microenvironment through TLR4/MD-2/MyD88-mediated NF-κB signaling.

    abstract::Increasing evidence from various clinical and experimental studies has demonstrated that the inflammatory microenvironment created by immune cells facilitates tumor migration. Epithelial-mesenchymal transition (EMT) is involved in the progression of cancer invasion and metastasis in an inflammatory microenvironment. B...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Ye K,Chen QW,Sun YF,Lin JA,Xu JH

    更新日期:2018-02-01 00:00:00

  • N-end rule pathway inhibitor sensitizes cancer cells to antineoplastic agents by regulating XIAP and RAD21 protein expression.

    abstract::Anticancer drugs exert their effects on cancer cells by deregulating many pathways linked to cell cycle, apoptosis, etc. but cancer cells gradually become resistive against anticancer drugs, thereby necessitating the development of newer generation anticancer molecules. N-end rule pathway has been shown to be involved...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Pore SK,Ganguly A,Sau S,Godeshala S,Kanugula AK,Ummanni R,Kotamraju S,Banerjee R

    更新日期:2020-01-01 00:00:00

  • Computational and mutational analysis of TatD DNase of Bacillus anthracis.

    abstract::The role of TatD DNases as DNA repair enzymes or cell death (apoptotic) nucleases is well established in prokaryotes as well as eukaryotes. The current study aims to characterize the TatD nuclease from Bacillus anthracis (Ba TatD) and to explore its key histidine catalytic residues. Ba TatD was found to be a metal-dep...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Singh D,Rahi A,Kumari R,Gupta V,Gautam G,Aggarwal S,Rehan M,Bhatnagar R

    更新日期:2019-02-05 00:00:00

  • Stimulatory effect of regucalcin on mitochondrial ATP-dependent calcium uptake activity in rat kidney cortex.

    abstract::The effect of regucalcin, which is a regulatory protein of Ca(2+) signaling, on Ca(2+)-ATPase activity in isolated rat renal cortex mitochondria was investigated. The presence of regucalcin (50, 100, and 250 nM) in the enzyme reaction mixture led to a significant increase in Ca(2+)-ATPase activity. Regucalcin signific...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Xue JH,Takahashi H,Yamaguchi M

    更新日期:2000-10-20 00:00:00