Anaerobic Degradation of the Plant Sugar Sulfoquinovose Concomitant With H2S Production: Escherichia coli K-12 and Desulfovibrio sp. Strain DF1 as Co-culture Model.


:Sulfoquinovose (SQ, 6-deoxy-6-sulfoglucose) is produced by plants and other phototrophs and its biodegradation is a relevant component of the biogeochemical carbon and sulfur cycles. SQ is known to be degraded by aerobic bacterial consortia in two tiers via C3-organosulfonates as transient intermediates to CO2, water and sulfate. In this study, we present a first laboratory model for anaerobic degradation of SQ by bacterial consortia in two tiers to acetate and hydrogen sulfide (H2S). For the first tier, SQ-degrading Escherichia coli K-12 was used. It catalyzes the fermentation of SQ to 2,3-dihydroxypropane-1-sulfonate (DHPS), succinate, acetate and formate, thus, a novel type of mixed-acid fermentation. It employs the characterized SQ Embden-Meyerhof-Parnas pathway, as confirmed by mutational and proteomic analyses. For the second tier, a DHPS-degrading Desulfovibrio sp. isolate from anaerobic sewage sludge was used, strain DF1. It catalyzes another novel fermentation, of the DHPS to acetate and H2S. Its DHPS desulfonation pathway was identified by differential proteomics and demonstrated by heterologously produced enzymes: DHPS is oxidized via 3-sulfolactaldehyde to 3-sulfolactate (SL) by two NAD+-dependent dehydrogenases (DhpA, SlaB); the SL is cleaved by an SL sulfite-lyase known from aerobic bacteria (SuyAB) to pyruvate and sulfite. The pyruvate is oxidized to acetate, while the sulfite is used as electron acceptor in respiration and reduced to H2S. In conclusion, anaerobic sulfidogenic SQ degradation was demonstrated as a novel link in the biogeochemical sulfur cycle. SQ is also a constituent of the green-vegetable diet of herbivores and omnivores and H2S production in the intestinal microbiome has many recognized and potential contributions to human health and disease. Hence, it is important to examine bacterial SQ degradation also in the human intestinal microbiome, in relation to H2S production, dietary conditions and human health.


Front Microbiol


Burrichter A,Denger K,Franchini P,Huhn T,Müller N,Spiteller D,Schleheck D




Has Abstract


2018-11-27 00:00:00








  • Diversity-Stability Dynamics of the Amphibian Skin Microbiome and Susceptibility to a Lethal Viral Pathogen.

    abstract::Variation among animals in their host-associated microbial communities is increasingly recognized as a key determinant of important life history traits including growth, metabolism, and resistance to disease. Quantitative estimates of the factors shaping the stability of host microbiomes over time at the individual le...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Harrison XA,Price SJ,Hopkins K,Leung WTM,Sergeant C,Garner TWJ

    更新日期:2019-12-20 00:00:00

  • Toll-like receptor 4 confers inflammatory response to Suilysin.

    abstract::Streptococcus suis serotype 2 (SS2) is an emerging human pathogen worldwide. A large outbreak occurred in the summer of 2005 in China. Serum samples from this outbreak revealed that levels of the main proinflammatory cytokines were significantly higher in patients with streptococcal toxic-shock-like syndrome (STSLS) t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Bi L,Pian Y,Chen S,Ren Z,Liu P,Lv Q,Zheng Y,Zhang S,Hao H,Yuan Y,Jiang Y

    更新日期:2015-06-26 00:00:00

  • Molecular Epidemiology of Staphylococcus epidermidis Implicated in Catheter-Related Bloodstream Infections at an Academic Hospital in Pretoria, South Africa.

    abstract::Staphylococcus epidermidis is one of the most prevalent pathogens implicated in catheter-related bloodstream infections (CRBSI) at an academic hospital in Pretoria, South Africa. Little is known about the clonality and the prevalence of antibiotic resistance and virulence genes in S. epidermidis (e.g., icaAB, IS256, m...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Ehlers MM,Strasheim W,Lowe M,Ueckermann V,Kock MM

    更新日期:2018-03-07 00:00:00

  • Benthic microbial communities of coastal terrestrial and ice shelf Antarctic meltwater ponds.

    abstract::The numerous perennial meltwater ponds distributed throughout Antarctica represent diverse and productive ecosystems central to the ecological functioning of the surrounding ultra oligotrophic environment. The dominant taxa in the pond benthic communities have been well described however, little is known regarding the...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Archer SD,McDonald IR,Herbold CW,Lee CK,Cary CS

    更新日期:2015-05-27 00:00:00

  • From Petri Dish to Patient: Bioavailability Estimation and Mechanism of Action for Antimicrobial and Immunomodulatory Natural Products.

    abstract::The new era of multidrug resistance of pathogens against frontline antibiotics has compromised the immense therapeutic gains of the 'golden age,' stimulating a resurgence in antimicrobial research focused on antimicrobial and immunomodulatory components of botanical, fungal or microbial origin. While much valuable inf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Sadgrove NJ,Jones GL

    更新日期:2019-10-31 00:00:00

  • Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity.

    abstract::Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiL...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Leastro MO,Castro DYO,Freitas-Astúa J,Kitajima EW,Pallás V,Sánchez-Navarro JÁ

    更新日期:2020-06-09 00:00:00

  • Staphylococcus aureus Isolates from Goat and Sheep Milk Seem to Be Closely Related and Differ from Isolates Detected from Bovine Milk.

    abstract::Dairy goat and sheep farms suffer severe economic losses due to intramammary infections, with Staphylococcus aureus representing the main cause of clinical mastitis in small ruminants. In addition, S. aureus contamination of goat and sheep milk may cause staphylococcal food poisoning, as many traditional caprine and o...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Merz A,Stephan R,Johler S

    更新日期:2016-03-14 00:00:00

  • Prevalence, Molecular Characterization, and Antibiotic Susceptibility of Cronobacter sakazakii Isolates from Powdered Infant Formula Collected from Chinese Retail Markets.

    abstract::Cronobacter sakazakii is an opportunistic pathogen that causes severe infections in neonates and infants through contaminated powdered infant formula (PIF). Therefore, the aim of this study was a large-scale study on determine the prevalence, molecular characterization and antibiotic susceptibility of C. sakazakii iso...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Fei P,Jiang Y,Jiang Y,Yuan X,Yang T,Chen J,Wang Z,Kang H,Forsythe SJ

    更新日期:2017-10-17 00:00:00

  • Vertical Beta-Diversity of Bacterial Communities Depending on Water Stratification.

    abstract::Many studies indicate that variation of marine bacterial beta diversity in the horizontal dimension is mainly attributable to environmental and spatial effects. However, whether and how these two effects drive bacterial beta diversity in the vertical dimension remains unclear, especially when considering seasonal vari...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Cheng WH,Lu HP,Chen CC,Jan S,Hsieh CH

    更新日期:2020-03-19 00:00:00

  • Corrigendum: Impact of Salicylic Acid and PGPR on the Drought Tolerance and Phytoremediation Potential of Helianthus annus.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2018.02507.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误


    authors: Khan N,Zandi P,Ali S,Mehmood A,Adnan Shahid M,Yang J

    更新日期:2019-09-27 00:00:00

  • Microbial Group Dynamics in Plant Rhizospheres and Their Implications on Nutrient Cycling.

    abstract::Plant rhizospheres encompass a dynamic zone of interactions between microorganisms and their respective plant hosts. For decades, researchers have worked to understand how these complex interactions influence different aspects of plant growth, development, and evolution. Studies of plant-microbial interactions in the ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Garcia J,Kao-Kniffin J

    更新日期:2018-07-11 00:00:00

  • No Effect of Microgravity and Simulated Mars Gravity on Final Bacterial Cell Concentrations on the International Space Station: Applications to Space Bioproduction.

    abstract::Microorganisms perform countless tasks on Earth and they are expected to be essential for human space exploration. Despite the interest in the responses of bacteria to space conditions, the findings on the effects of microgravity have been contradictory, while the effects of Martian gravity are nearly unknown. We perf...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Santomartino R,Waajen AC,de Wit W,Nicholson N,Parmitano L,Loudon CM,Moeller R,Rettberg P,Fuchs FM,Van Houdt R,Finster K,Coninx I,Krause J,Koehler A,Caplin N,Zuijderduijn L,Zolesi V,Balsamo M,Mariani A,Pellari SS,C

    更新日期:2020-10-14 00:00:00

  • Serotype Distribution and Antimicrobial Susceptibility of Streptococcus pneumoniae in Pre- and Post- PCV7/13 Eras, Taiwan, 2002-2018.

    abstract::In Taiwan, the 7-valent pneumococcal conjugate vaccine (PCV7) was introduced in 2006 and a PCV13 national childhood catchup program was implemented in 2013. To delineate the trend of serotype distribution and antimicrobial susceptibility following vaccination programs, we investigated a total of 1845 Streptococcus pne...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Wu CJ,Lai JF,Huang IW,Shiau YR,Wang HY,Lauderdale TL

    更新日期:2020-10-22 00:00:00

  • Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review.

    abstract::Aspergillus exists commonly in many crops and any process of crop growth, harvest, storage, and processing can be polluted by this fungus. Once it forms a biofilm, Aspergillus can produce many toxins, such as aflatoxin B1 (AFB1), ochratoxin, zearalenone, fumonisin, and patulin. Among these toxins, AFB1 possesses the h...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审


    authors: Wang Q,Yang Q,Wu W

    更新日期:2020-03-27 00:00:00

  • Corrigendum: Living at the Extremes: Extremophiles and the Limits of Life in a Planetary Context.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2019.00780.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误


    authors: Merino N,Aronson HS,Bojanova DP,Feyhl-Buska J,Wong ML,Zhang S,Giovannelli D

    更新日期:2019-08-13 00:00:00

  • Double Maximum Ratios of Viruses to Bacteria in the Water Column: Implications for Different Regulating Mechanisms.

    abstract::The viruses play an important role in limiting bacterial abundance in oceans and, hence, in regulating bacterial biogeochemical functions. A cruise was conducted in September 2005 along a transect in the deep South China Sea (SCS). The results showed the double maxima in the ratio of viral to bacterial abundance (VBR)...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: He L,Yin K,Yuan X

    更新日期:2019-07-16 00:00:00

  • Bacterial Communities of the Canola Rhizosphere: Network Analysis Reveals a Core Bacterium Shaping Microbial Interactions.

    abstract::The rhizosphere hosts a complex web of prokaryotes interacting with one another that may modulate crucial functions related to plant growth and health. Identifying the key factors structuring the prokaryotic community of the plant rhizosphere is a necessary step toward the enhancement of plant production and crop yiel...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Floc'h JB,Hamel C,Lupwayi N,Harker KN,Hijri M,St-Arnaud M

    更新日期:2020-07-10 00:00:00

  • Evolutionary Implications of Anoxygenic Phototrophy in the Bacterial Phylum Candidatus Eremiobacterota (WPS-2).

    abstract::Genome-resolved environmental metagenomic sequencing has uncovered substantial previously unrecognized microbial diversity relevant for understanding the ecology and evolution of the biosphere, providing a more nuanced view of the distribution and ecological significance of traits including phototrophy across diverse ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Ward LM,Cardona T,Holland-Moritz H

    更新日期:2019-07-23 00:00:00

  • First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile.

    abstract::The VPAP30 strain was isolated as the highly predominant bacteria from an episode of massive larval mortality occurring in a commercial culture of the Chilean scallop Argopecten purpuratus. The main aims of this study were, to characterize and identify the pathogenic strain using biochemical and molecular methods to d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,收录出版


    authors: Rojas R,Miranda CD,Santander J,Romero J

    更新日期:2016-09-20 00:00:00

  • Hyper-Osmotic Stress Elicits Membrane Depolarization and Decreased Permeability in Halotolerant Marine Debaryomyces hansenii Strains and in Saccharomyces cerevisiae.

    abstract::The use of seawater and marine microorganisms can represent a sustainable alternative to avoid large consumption of freshwater performing industrial bioprocesses. Debaryomyces hansenii, which is a known halotolerant yeast, possess metabolic traits appealing for developing such processes. For this purpose, we studied s...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Capusoni C,Arioli S,Donzella S,Guidi B,Serra I,Compagno C

    更新日期:2019-01-29 00:00:00

  • Prevalence of Antibiotic and Heavy Metal Resistance Determinants and Virulence-Related Genetic Elements in Plasmids of Staphylococcus aureus.

    abstract::The use of antibiotics on a mass scale, particularly in farming, and their release into the environment has led to a rapid emergence of resistant bacteria. Once emerged, resistance determinants are spread by horizontal gene transfer among strains of the same as well as disparate bacterial species. Their accumulation i...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Bukowski M,Piwowarczyk R,Madry A,Zagorski-Przybylo R,Hydzik M,Wladyka B

    更新日期:2019-04-24 00:00:00

  • Environmental Pollutant Benzo[a]Pyrene Impacts the Volatile Metabolome and Transcriptome of the Human Gut Microbiota.

    abstract::Benzo[a]pyrene (B[a]P) is a ubiquitous, persistent, and carcinogenic pollutant that belongs to the large family of polycyclic aromatic hydrocarbons. Population exposure primarily occurs via contaminated food products, which introduces the pollutant to the digestive tract. Although the metabolism of B[a]P by host cells...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Defois C,Ratel J,Denis S,Batut B,Beugnot R,Peyretaillade E,Engel E,Peyret P

    更新日期:2017-08-15 00:00:00

  • Rhythmic and sustained oscillations in metabolism and gene expression of Cyanothece sp. ATCC 51142 under constant light.

    abstract::Cyanobacteria, a group of photosynthetic prokaryotes, oscillate between day and night time metabolisms with concomitant oscillations in gene expression in response to light/dark cycles (LD). The oscillations in gene expression have been shown to sustain in constant light (LL) with a free running period of 24 h in a mo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Gaudana SB,Krishnakumar S,Alagesan S,Digmurti MG,Viswanathan GA,Chetty M,Wangikar PP

    更新日期:2013-12-06 00:00:00

  • Genetic Characterization of a bla VIM-24-Carrying IncP-7β Plasmid p1160-VIM and a bla VIM-4-Harboring Integrative and Conjugative Element Tn6413 From Clinical Pseudomonas aeruginosa.

    abstract::This study presents three novel integrons In1394, In1395, and In1443, three novel unit transposons Tn6392, Tn6393, and Tn6403, one novel conjugative element (ICE) Tn6413, and the first sequenced IncP-7 resistance plasmid p1160-VIM from clinical Pseudomonas aeruginosa. Detailed sequence comparison of p1160-VIM (carryin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Zeng L,Zhan Z,Hu L,Jiang X,Zhang Y,Feng J,Gao B,Zhao Y,Yang W,Yang H,Yin Z,Zhou D

    更新日期:2019-02-26 00:00:00

  • Building of Pressure-Assisted Ultra-High Temperature System and Its Inactivation of Bacterial Spores.

    abstract::The pressure-assisted ultra-high temperature (PAUHT) system was built by using soybean oil as pressure-transmitting medium, and the multiple regression equation of soybean oil temperature change (ΔTP ) during pressurization as a function of initial temperature (Ti ) and set pressure (P) was developed: ΔTP = -13.45 + 0...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Liang D,Zhang L,Wang X,Wang P,Liao X,Wu X,Chen F,Hu X

    更新日期:2019-06-10 00:00:00

  • Immunodeficiency Promotes Adaptive Alterations of Host Gut Microbiome: An Observational Metagenomic Study in Mice.

    abstract::The crosstalk between the gut microbiota and immune state of the host is an essential focus in academia and clinics. To explore the dynamic role of the microbiota in response to immune deficiency, we comprehensively assessed the microbiome of 90 mouse fecal samples, across three time points including two immunodeficie...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Zheng S,Zhao T,Yuan S,Yang L,Ding J,Cui L,Xu M

    更新日期:2019-11-01 00:00:00

  • QseC Mediates Osmotic Stress Resistance and Biofilm Formation in Haemophilus parasuis.

    abstract::Haemophilus parasuis is known as a commensal organism discovered in the upper respiratory tract of swine where the pathogenic bacteria survive in various adverse environmental stress. QseC, a histidine protein kinase of the two-component regulatory systems CheY/QseC, is involved in the environmental adaptation in bact...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: He L,Dai K,Wen X,Ding L,Cao S,Huang X,Wu R,Zhao Q,Huang Y,Yan Q,Ma X,Han X,Wen Y

    更新日期:2018-02-13 00:00:00

  • Deletion of the Small RNA Chaperone Protein Hfq down Regulates Genes Related to Virulence and Confers Protection against Wild-Type Brucella Challenge in Mice.

    abstract::Brucellosis is one of the most common zoonotic epidemics worldwide. Brucella, the etiological pathogen of brucellosis, has unique virulence characteristics, including the ability to survive within the host cell. Hfq is a bacterial chaperone protein that is involved in the survival of the pathogen under stress conditio...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Lei S,Zhong Z,Ke Y,Yang M,Xu X,Ren H,An C,Yuan J,Yu J,Xu J,Qiu Y,Shi Y,Wang Y,Peng G,Chen Z

    更新日期:2016-01-20 00:00:00

  • Catabolism of Alkylphenols in Rhodococcus via a Meta-Cleavage Pathway Associated With Genomic Islands.

    abstract::The bacterial catabolism of aromatic compounds has considerable promise to convert lignin depolymerization products to commercial chemicals. Alkylphenols are a key class of depolymerization products whose catabolism is not well-elucidated. We isolated Rhodococcus rhodochrous EP4 on 4-ethylphenol and applied genomic an...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Levy-Booth DJ,Fetherolf MM,Stewart GR,Liu J,Eltis LD,Mohn WW

    更新日期:2019-08-20 00:00:00

  • Cathelicidin-Derived Synthetic Peptide Improves Therapeutic Potential of Vancomycin Against Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa (PA) is the leading cause of corneal blindness worldwide. A constant increase in multi-drug resistant PA strains have heightened the challenge of effectively managing corneal infections with conventional antibiotics. Antimicrobial peptides are promising antibiotic analogs with a unique mode of a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章


    authors: Mohammed I,Said DG,Nubile M,Mastropasqua L,Dua HS

    更新日期:2019-09-19 00:00:00