MicroRNA-205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1.


:MicroRNA-205 (miR-205) is involved in various physiological and pathological processes, but its biological function in follicular atresia remains unclear. In this study, we investigated miR-205 expression in mouse granulosa cells (mGCs) and analyzed its functions in primary mGCs by performing a series of in vitro experiments. Quantitative real-time polymerase chain reaction showed that miR-205 expression was significantly higher in early atretic follicles and progressively atretic follicles than in healthy follicles. miR-205 overexpression in mGCs significantly promoted apoptosis and caspase-3/9 activities, as well as inhibited estrogen (E2) release and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene encoding cyclic AMP response element (CRE)-binding protein 1 (CREB1) was a direct target of miR-205 in mGCs. CREB1 upregulation partially rescued the effects of miR-205 on apoptosis, caspase-3/9 activities, E2 production, and CYP19A1 expression on mGCs. These results indicate that miR-205 might play an important role in ovarian follicular development and provide new insights into follicular atresia.


J Cell Biochem


Zhang P,Wang J,Lang H,Wang W,Liu X,Liu H,Tan C,Li X,Zhao Y,Wu X




Has Abstract


2018-12-16 00:00:00






  • Atorvastatin stimulates the production of osteoprotegerin by human osteoblasts.

    abstract::Recently, HMG-CoA reductase inhibitors (statins), potent inhibitors of cholesterol biosynthesis, have been linked to protective effects on bone metabolism. Because of their widespread use, prevention of bone loss and fractures would be a desirable side effect. However, the mechanisms how statins may affect bone metabo...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Viereck V,Gründker C,Blaschke S,Frosch KH,Schoppet M,Emons G,Hofbauer LC

    更新日期:2005-12-15 00:00:00

  • Effects of selenium supplementation on expression of SEPP1 in mRNA and protein levels in subjects with and without metabolic syndrome suffering from coronary artery disease: Selenegene study a double-blind randomized controlled trial.

    abstract::Selenoprotein P (SePP) is involved in the protection against diseases. The present study is the first investigation of the effect of selenium supplementation on plasma selenium and expression of SEPP1 in mRNA and protein levels based on metabolic syndrome (MetS), in individuals suffering from coronary artery diseases....

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,随机对照试验


    authors: Gharipour M,Ouguerram K,Nazih EH,Salehi M,Behmanesh M,Roohafza H,Hosseini SM,Nezafati P,Dianatkhah M,Gharipour A,Haghjoo S,Sarrafzadegan N,Sadeghi M

    更新日期:2018-11-01 00:00:00

  • Sorafenib induces endometrial carcinoma apoptosis by inhibiting Elk-1-dependent Mcl-1 transcription and inducing Akt/GSK3β-dependent protein degradation.

    abstract::Endometrial carcinoma (EC) is one of the main gynecologic malignancies affecting women, but effective treatments are currently lacking. In the present study, we investigated the effect of sorafenib, a general kinase inhibitor, on several EC cell lines (HEC1A, HEC1B, and RL95-2). Sorafenib induced cell death in EC cell...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sun NK,Huang SL,Chang TC,Chao CC

    更新日期:2013-08-01 00:00:00

  • Increasing and decreasing protein stability: effects of revertant substitutions on the thermal denaturation of phage lambda repressor.

    abstract::The thermal denaturations of five revertant lambda repressors containing single amino acid substitutions in their N-terminal domains have been studied by differential scanning calorimetry. Two substitutions slightly decrease stability, and the remaining three render the protein more stable than wild type. The Gly48---...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Hecht MH,Hehir KM,Nelson HC,Sturtevant JM,Sauer RT

    更新日期:1985-01-01 00:00:00

  • DNA methylation in embryonic stem cells.

    abstract::Embryonic stem cells (ESCs) are pluripotent, self-renewing cells. These cells can be used in applications such as cell therapy, drug development, disease modeling, and the study of cellular differentiation. Investigating the interplay of epigenetics, genetics, and gene expression in control of pluripotence and differe...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Altun G,Loring JF,Laurent LC

    更新日期:2010-01-01 00:00:00

  • Upregulated microRNA-485 suppresses apoptosis of renal tubular epithelial cells in mice with lupus nephritis via regulating the TGF-β-MAPK signaling pathway by inhibiting RhoA expression.

    abstract::Lupus nephritis (LN) is a common and severe complication of systemic lupus erythematosus. Without intervention, LN may cause acute kidney injury and end-stage renal disease. This study aims to determine whether microRNA-485 (miR-485) affects renal tubular epithelial cells (RTECs) in LN mice via the TGF-β-MAPK signalin...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Tian Y,Han YX,Guo HF,Jin HT,Sun C,Qi X,Ma LY,Bo SW

    更新日期:2018-11-01 00:00:00

  • Soluble 80-kd fragment of cell-CAM 120/80 disrupts cell-cell adhesion.

    abstract::Calcium-dependent cell adhesion molecules (CAMs) mediate intercellular adhesion in epithelial cells and in preimplantation mammalian embryos. One of these molecules, cell-CAM 120/80, is found on cells as a 120-kd membrane glycoprotein and as a soluble 80-kd species in conditioned culture medium [Damsky et al: Cell 34:...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Wheelock MJ,Buck CA,Bechtol KB,Damsky CH

    更新日期:1987-07-01 00:00:00

  • Nuclear ultrastructures associated with the RNA synthesis and processing.

    abstract::Relatively little is known about the spatial organization of RNA synthesis, processing, and transport in (mammalian) cell nuclei. This review summarizes results of electron microscopic mapping of RNA synthetic sites and macromolecules involved directly, or indirectly, in the metabolism of RNAs in somatic cell mammalia...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Raska I

    更新日期:1995-09-01 00:00:00

  • MiR-377 Regulates Inflammation and Angiogenesis in Rats After Cerebral Ischemic Injury.

    abstract::Ischemic stroke is the leading cause of disabilities worldwide. MicroRNA-377 (miR-377) plays important roles in ischemic injury. The present study focused on the mechanisms of miR-377 in protecting ischemic brain injury in rats. Cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in rats. Primary ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Fan Y,Ding S,Sun Y,Zhao B,Pan Y,Wan J

    更新日期:2018-01-01 00:00:00

  • Protective effect of hypoglycemic granule against diabetes-induced liver injury by alleviating glycolipid metabolic disorder and oxidative stress.

    abstract::The current study was designed to explore the therapeutic effect and mechanism of different extraction, which came from hypoglycemic granule on diabetes-induced liver injury. The ethanol fraction (HGEF) and aqueous fraction (HGAF) from hypoglycemic granule were prepared and administered p.o. to diabetic mice for 17 we...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yang X,Zhang P,Zhang F,Ke Z,Chen Q,Liu C

    更新日期:2020-06-01 00:00:00

  • Pyrosequencing for accurate imprinted allele expression analysis.

    abstract::Genomic imprinting is an epigenetic mechanism that restricts gene expression to one inherited allele. Improper maintenance of imprinting has been implicated in a number of human diseases and developmental syndromes. Assays are needed that can quantify the contribution of each paternal allele to a gene expression profi...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yang B,Damaschke N,Yao T,McCormick J,Wagner J,Jarrard D

    更新日期:2015-07-01 00:00:00

  • Activation of c-Jun NH2-terminal kinases by interleukin-1 beta in normal human osteoblastic and rat UMR-106 cells.

    abstract::We recently demonstrated the activation of extracellular signal- regulated protein kinase 1 and 2 (ERK1 and ERK2) by IGF-1, FGF-2, and PDGF-BB in normal human osteoblastic (HOB) cells as well as in rat and mouse osteoblastic cells. In this report, we have examined whether c-Jun NH2-Terminal Kinase (JNK) pathway is act...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Chaudhary LR,Avioli LV

    更新日期:1998-04-01 00:00:00

  • Cyr61 is up-regulated in prostate cancer and associated with the p53 gene status.

    abstract::Cysteine-rich 61 (Cyr61) is a member of the CCN protein family that has been implicated in diverse biological processes such as cell adhesion, proliferation, angiogenesis, and tumorigenesis. Altered expression of Cyr61 is found to be associated with human cancers. Here we show that Cyr61 was up-regulated in prostate c...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Lv H,Fan E,Sun S,Ma X,Zhang X,Han DM,Cong YS

    更新日期:2009-03-01 00:00:00

  • lncRNA expression signatures in periodontitis revealed by microarray: the potential role of lncRNAs in periodontitis pathogenesis.

    abstract::Periodontitis, a common chronic inflammatory disease of the periodontium, is caused by dental plaque formation induced by microorganisms. Recent studies have demonstrated that lncRNAs play a critical role in the regulation of gene expression and in the pathogenesis of diseases. To demonstrate that periodontitis is ass...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zou Y,Li C,Shu F,Tian Z,Xu W,Xu H,Tian H,Shi R,Mao X

    更新日期:2015-04-01 00:00:00

  • Constitutive and inducible expression of cytochromes P4501A (CYP1A1 and CYP1A2) in normal prostate and prostate cancer cells.

    abstract::Constitutive and benzo[a]pyrene (B[a]P) inducible expression of CYP1A1 and CYP1A2 in prostate cancer and normal prostate epithelial cells were examined by immunoblotting. Androgen independent prostate cancer cell lines DU145 and PC3 have constitutive expression of CYP1A and CYP1A1 and CYP1A2, respectively. Four microm...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Sterling KM Jr,Cutroneo KR

    更新日期:2004-02-01 00:00:00

  • Extracellular Signal-Regulated Protein Kinase, c-Jun N-Terminal Protein Kinase, and Calcineurin Regulate Transient Receptor Potential M3 (TRPM3) Induced Activation of AP-1.

    abstract::Stimulation of transient receptor potential M3 (TRPM3) cation channels with pregnenolone sulfate induces an influx of Ca2+ ions into the cells and a rise in the intracellular Ca2+ concentration, leading to the activation of the activator protein-1 (AP-1) transcription factor. Here, we show that expression of a constit...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Lesch A,Rössler OG,Thiel G

    更新日期:2017-08-01 00:00:00

  • Dysregulated Metabolism of the Amyloid-β Protein and Therapeutic Approaches in Alzheimer Disease.

    abstract::Amyloid-β protein (Aβ) is the main component of senile plaques in the brains of Alzheimer disease (AD) patients. Aβ is proteolytically derived from amyloid-β precursor protein by β- and γ-secretases. Secreted Aβ is then eliminated from the central nervous system by multiple clearance mechanisms, including phagocytosis...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Kikuchi K,Kidana K,Tatebe T,Tomita T

    更新日期:2017-12-01 00:00:00

  • 4-Deoxyraputindole C induces cell death and cell cycle arrest in tumor cell lines.

    abstract::Several molecules extracted from natural products exhibit different biological activities, such as ion channel modulation, activation of signaling pathways, and anti-inflammatory or antitumor activity. In this study, we tested the antitumor ability of natural compounds extracted from the Raputia praetermissa plant. Am...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Vital WD,Torquato HFV,Jesus LOP,Judice WAS,Silva MFDGFD,Rodrigues T,Justo GZ,Veiga TAM,Paredes-Gamero EJ

    更新日期:2019-06-01 00:00:00

  • Overlapped differentially expressed genes between acute lymphoblastic leukemia and chronic lymphocytic leukemia revealed potential key genes and pathways involved in leukemia.

    abstract::Common differentially expressed genes (DEGs) in acute lymphoblastic leukemia (ALL) and chronic lymphocytic leukemia (CLL) might play critical roles in the pathogenesis and process of leukemia. We collected RNA sequencing (RNA-seq) data of human CLL, ALL samples, and normal peripheral blood CD19+ B cells as well as thy...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Zhang S,Zhang Q,Yin J,Wu X

    更新日期:2019-09-01 00:00:00

  • Conversion of immortal liver progenitor cells into pancreatic endocrine progenitor cells by persistent expression of Pdx-1.

    abstract::The conversion of expandable liver progenitor cells into pancreatic beta cells would provide a renewable cell source for diabetes cell therapy. Previously, we reported the establishment of liver epithelial progenitor cells (LEPCs). In this work, LEPCs were modified into EGFP/Pdx-1 LEPCs, cells with stable expression o...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Jin CX,Li WL,Xu F,Geng ZH,He ZY,Su J,Tao XR,Ding XY,Wang X,Hu YP

    更新日期:2008-05-01 00:00:00

  • Ultraviolet radiation (UVR) activates p38 MAP kinase and induces post-transcriptional stabilization of the C/EBPdelta mRNA in G0 growth arrested mammary epithelial cells.

    abstract::The G(0) growth arrest (quiescent) state is highly conserved in evolution to promote survival under adverse environmental conditions. To maintain viability, G(0) growth arrested cells limit gene expression to essential growth control and pro-survival genes. CCAAT enhancer binding protein delta (C/EBPdelta), a member o...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Li B,Si J,DeWille JW

    更新日期:2008-04-01 00:00:00

  • Evaluate the Efficacy and Safety of Anti-Epileptic Medications for Partial Seizures of Epilepsy: A Network Meta-Analysis.

    abstract::Epilepsy is a brain and neurological disorder with high prevalence. It was reported that more than 70% of epileptic seizures were controlled by anti-epileptic medications, whereas the lack of evidence with respect to head-to-head comparisons motivated researchers to seek alternative approaches that are able to provide...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,meta分析,评审


    authors: Zhao T,Feng X,Liu J,Gao J,Zhou C

    更新日期:2017-09-01 00:00:00

  • Stat1-mediated cytoplasmic attenuation in osteoimmunology.

    abstract::Signal transducer and activator of transcription 1 (Stat1) is a critical mediator of gene transcription in type I interferon (IFN-alpha/beta) signaling that is essential for host defense against viruses. In the skeletal system, type I IFNs (IFN-alpha/beta) also play an important physiological role in the inhibition of...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Takayanagi H,Kim S,Koga T,Taniguchi T

    更新日期:2005-02-01 00:00:00

  • Analgesic effects of microRNA-129-5p against bone cancer pain through the EphB1/EphrinB2 signaling pathway in mice.

    abstract::The study aims to investigate the analgesic effects of microRNA-129-5p (miR-129-5p) on bone cancer pain (BCP) by targeting Eph receptor B1 (EphB1) through the EphB1/EphrinB2 signaling pathway. BCP mice models were established, and C3H/HeJ female mice were classified into the normal, blank, negative control (NC), miR-1...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Yu SN,Liu GF,Li LY,Zhao GQ,Liu L,Li XF

    更新日期:2019-03-01 00:00:00

  • E-, P-, and N-cadherin are co-expressed in the nasopharyngeal carcinoma cell line TW-039.

    abstract::The cadherin/catenin complex plays a key role in the initiation of cell-cell recognition, and adhesion, and the elaboration of structural and functional organization in multicellular tissues and organs. It is associated with tumor metastasis and also acts as an "invasion suppressor" of cancer cells. Nasopharyngeal car...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Lou PJ,Chen WP,Lin CT,DePhilip RM,Wu JC

    更新日期:1999-11-01 00:00:00

  • Integrin alpha V beta 3 targeted dendrimer-rapamycin conjugate reduces fibroblast-mediated prostate tumor progression and metastasis.

    abstract::Therapeutic strategies targeting both cancer cells and associated cells in the tumor microenvironment offer significant promise in cancer therapy. We previously reported that generation 5 (G5) dendrimers conjugated with cyclic-RGD peptides target cells expressing integrin alpha V beta 3. In this study, we report a nov...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Hill EE,Kim JK,Jung Y,Neeley CK,Pienta KJ,Taichman RS,Nor JE,Baker JR Jr,Krebsbach PH

    更新日期:2018-11-01 00:00:00

  • Expression of bone-specific genes by hypertrophic chondrocytes: implication of the complex functions of the hypertrophic chondrocyte during endochondral bone development.

    abstract::Endochondral bone formation is one of the most extensively examined developmental sequences within vertebrates. This process involves the coordinated temporal/spatial differentiation of three separate tissues (cartilage, bone, and the vasculature) into a variety of complex structures. The differentiation of chondrocyt...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Gerstenfeld LC,Shapiro FD

    更新日期:1996-07-01 00:00:00

  • The decreased lncRNA ZEB2-AS1 in pre-eclampsia controls the trophoblastic cell line HTR-8/SVneo's invasive and migratory abilities via the miR-149/PGF axis.

    abstract::Pre-eclampsia (PE) is a pregnancy disease that causes maternal death and threatens the health of newborns. Accumulating evidence has revealed the essential roles of long noncoding RNAs (lncRNAs) in the progression of PE. The present investigation determined lncRNA ZEB2 antisense RNA 1 (ZEB2-AS1) expression in PE and l...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Gao Y,Guo X,Li Y,Sha W,She R

    更新日期:2019-10-01 00:00:00

  • Amino-terminus domain of the androgen receptor as a molecular target to prevent the hormonal progression of prostate cancer.

    abstract::Prostate cancer has a propensity to metastasize to the bone. Currently the only effective systemic treatment for these patients is androgen ablation therapy. However, the tumor will invariably progress to an androgen-independent stage and the patient will succumb to his disease within approximately 2 years. The earlie...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审


    authors: Wang G,Sadar MD

    更新日期:2006-05-01 00:00:00

  • Biochemical and functional changes of rat liver spheroids during spheroid formation and maintenance in culture: I. morphological maturation and kinetic changes of energy metabolism, albumin synthesis, and activities of some enzymes.

    abstract::In the process of isolated single liver cells coming together to form three-dimensional spheroids, cells undergo dramatic environmental changes. How liver cells respond to these changes has not been well studied before. This study characterized the functional and biochemical changes during liver spheroid formation and...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章


    authors: Ma M,Xu J,Purcell WM

    更新日期:2003-12-15 00:00:00