Abstract:
:Therapeutically exploiting vascular and metabolic endpoints becomes critical to translational cancer studies because altered vascularity and deregulated metabolism are two important cancer hallmarks. The metabolic and vascular phenotypes of three sibling breast tumor lines with different metastatic potential are investigated in vivo with a newly developed quantitative spectroscopy system. All tumor lines have different metabolic and vascular characteristics compared to normal tissues, and there are strong positive correlations between metabolic (glucose uptake and mitochondrial membrane potential) and vascular (oxygen saturations and hemoglobin concentrations) parameters for metastatic (4T1) tumors but not for micrometastatic (4T07) and nonmetastatic (67NR) tumors. A longitudinal study shows that both vascular and metabolic endpoints of 4T1 tumors increased up to a specific tumor size threshold beyond which these parameters decreased. The synchronous changes between metabolic and vascular parameters, along with the strong positive correlations between these endpoints suggest that 4T1 tumors rely on strong oxidative phosphorylation in addition to glycolysis. This study illustrates the great potential of our optical technique to provide valuable dynamic information about the interplay between the metabolic and vascular status of tumors, with important implications for translational cancer investigations.
journal_name
J Biophotonicsjournal_title
Journal of biophotonicsauthors
Zhu C,Li M,Vincent T,Martin HL,Crouch BT,Martinez AF,Madonna MC,Palmer GM,Dewhirst MW,Ramanujam Ndoi
10.1002/jbio.201800372subject
Has Abstractpub_date
2019-04-01 00:00:00pages
e201800372issue
4eissn
1864-063Xissn
1864-0648journal_volume
12pub_type
杂志文章abstract::A multiple exposure laser speckle contrast imaging (MELSCI) setup for visualizing blood perfusion was developed using a field programmable gate array (FPGA), connected to a 1000 frames per second (fps) 1-megapixel camera sensor. Multiple exposure time images at 1, 2, 4, 8, 16, 32 and 64 milliseconds were calculated by...
journal_title:Journal of biophotonics
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abstract::A conceptually new type of corneal inlays for a customized treatment of presbyopia is presented. The diffractive inlay consists on a small aperture disc having an array of micro-holes distributed inside the open zones of a Fresnel zone plate. In this way, the central hole of the disc lets pass the zero order diffracti...
journal_title:Journal of biophotonics
pub_type: 信件
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abstract::Surface-enhanced Raman scattering (SERS) spectroscopy possesses the most promising advantage of multiplex detection for biosensing applications, which is achieved due to the narrow 'fingerprint' Raman spectra from the analyte molecules. We developed an ultrasensitive platform for the multiplex detection of cancer biom...
journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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abstract::One of the challenges in transcranial low-level laser therapy (LLLT) is to optimally choose illumination parameters, such as wavelength. However, there is sparse study on the wavelengths comparison especially on human transcranial LLLT. Here, we employed Monte Carlo modeling and visible human phantom to compute the pe...
journal_title:Journal of biophotonics
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abstract::The use of conventional fluorescence microscopy for characterizing tissue pathological states is limited by overlapping spectra and the dependence on excitation power and fluorophore concentration. Fluorescence lifetime imaging microscopy (FLIM) can overcome these limitations due to its insensitivity to fluorophore co...
journal_title:Journal of biophotonics
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abstract::We present a pseudo-real-time retinal layer segmentation for high-resolution Sensorless Adaptive Optics-Optical Coherence Tomography (SAO-OCT). Our pseudo-real-time segmentation method is based on Dijkstra's algorithm that uses the intensity of pixels and the vertical gradient of the image to find the minimum cost in ...
journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700123
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journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.200910052
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journal_title:Journal of biophotonics
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doi:10.1002/jbio.201300190
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journal_title:Journal of biophotonics
pub_type: 杂志文章,评审
doi:10.1002/jbio.201300198
更新日期:2015-01-01 00:00:00
abstract::Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label-free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201700402
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abstract::A robust method is presented for evaluating the diffusion properties of chemicals in ex vivo biological tissues. Using this method that relies only on thickness and collimated transmittance measurements, the diffusion properties of glycerol, fructose, polypropylene glycol and water in muscle tissues were evaluated. Am...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800333
更新日期:2019-04-01 00:00:00
abstract::Skin fibrosis, often referred to as skin scarring, is a significant international health problem with limited treatment options. The hallmarks of skin fibrosis are increased fibroblast proliferation, collagen production, and migration speed. Recently published clinical observations indicate that visible red light may ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201600059
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journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201800201
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journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201500315
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abstract::Lasers are used in dentistry for a variety of indications. One of these is the disinfection of root canals or the sterilization of residual caries. Many studies have demonstrated the capacity to kill bacteria for lasers but the fundamental mechanism of the laser effect remains quite unclear. With our experiments we wa...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201000007
更新日期:2010-06-01 00:00:00
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journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300119
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
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journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201200079
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abstract::The research work presented in this paper focuses on qualitative tissue differentiation by monitoring the intensity ratios of atomic emissions using 'Laser Induced Breakdown Spectroscopy' (LIBS) on the plasma plume created during laser tissue ablation. The background of this study is to establish a real time feedback ...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.201300159
更新日期:2015-01-01 00:00:00
abstract::To this day, any photosensitizers for the photodynamic treatment of pulmonary illnesses have been administered intravenously. There is, however, an intrinsic difficulty in reaching the target cells or bacteria in the respiratory system. Nebulization could overcome distribution problems and alleviate side effects by de...
journal_title:Journal of biophotonics
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abstract::The content of dermal beta-carotene can be a good indicator showing the body health. Because, it is involved in production of vitamin A maintaining healthy skin and mucous membranes. Also, it reduces the risk of cardiovascular diseases and its antioxidant capacity prevents the formation of cancerous cells. In this wor...
journal_title:Journal of biophotonics
pub_type: 杂志文章
doi:10.1002/jbio.202000124
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abstract::Near-infrared light allows measuring tissue oxygenation. These measurements relay on oxygenation-dependent absorption spectral changes. However, the tissue scattering, which is also spectral dependent, introduces an intrinsic error. Most methods focus on the volume reflectance from a semi-infinite sample. We have prop...
journal_title:Journal of biophotonics
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abstract::This study presents numerical simulations of UVC light propagation through seven different filtered face respirators (FFR) to determine their suitability for Ultraviolet germicidal inactivation (UVGI). UV propagation was modeled using the FullMonte program for two external light illuminations. The optical properties o...
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abstract::The purpose of this study was to evaluate thermal and near-infrared (NIR) reflectance imaging methods for the assessment of the activity of root caries lesions. In addition, changes in the lesion structure were monitored with polarization sensitive optical coherence tomography (PS-OCT). Artificial bovine and natural r...
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