Abstract:
BACKGROUND:Methods to improve the clinical efficacy of currently available antibiotics against multidrug resistant bacteria in cystic fibrosis (CF) chronic rhinosinusitis (CRS) are greatly needed. Ivacaftor, a cystic fibrosis transmembrane conductance regulator potentiator, was recently identified as having potentially beneficial off-target effects as a weak inhibitor of bacterial DNA gyrase and topoisomerase IV. The objective of the current study is to evaluate whether ivacaftor enhances the antimicrobial activity of ciprofloxacin against Pseudomonas aeruginosa. METHODS:The planktonic growth of the PAO-1 strain of P. aeruginosa was studied in the presence of ciprofloxacin and/or ivacaftor. Effects were measured according to optical density of cultured PAO-1 at 600 nm. For a static PAO-1 biofilm assay, the PAO-1 strain was inoculated and cultured for 72 h in the presence of the drugs. Formed PAO-1 biofilms were quantified by crystal violet staining and imaged with confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). RESULTS:PAO-1 growth was significantly reduced in the presence of ivacaftor (8 or 16 µg/mL) and ciprofloxacin (0.02 or 0.05 µg/mL) compared to ciprofloxacin alone ( P < .001). Similarly, ivacaftor (8 or 16 µg/mL) showed a significant reduction of PAO-1 biofilms when treated with 0.05 µg/mL of ciprofloxacin. Significant synergism was noted between ciprofloxacin and 16 µg/mL of ivacaftor ( P < .0001) in reducing planktonic growth and biofilm formation. Quantitative measurements with crystal violet staining were correlated to CLSM and SEM images. CONCLUSION:Ivacaftor enhanced ciprofloxacin's antimicrobial activity against P. aeruginosa. Further studies evaluating the efficacy of ivacaftor/ciprofloxacin combination for P. aeruginosa for CF CRS are warranted.
journal_name
Am J Rhinol Allergyjournal_title
American journal of rhinology & allergyauthors
Cho DY,Lim DJ,Mackey C,Skinner D,Zhang S,McCormick J,Woodworth BAdoi
10.1177/1945892418815615subject
Has Abstractpub_date
2019-03-01 00:00:00pages
129-136issue
2eissn
1945-8924issn
1945-8932journal_volume
33pub_type
杂志文章abstract:BACKGROUND:Ginseng (Panax ginseng C.A. Meyer) has been used in Asian countries for the treatment of various diseases. However, the mechanisms of liquid Panax ginseng (LG) on allergic inflammatory response in epidermal growth factor (EGF)-stimulated human airway epithelial cells remain largely unclear. METHODS:MUC5AC, ...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2011.25.3586
更新日期:2011-03-01 00:00:00
abstract:BACKGROUND:This study was designed to determine whether there is any correlation between results of the skin-prick test and the severity of symptoms in allergic rhinitis. METHODS:We retrospectively evaluated 150 patients with persistent or intermittent allergic rhinitis confirmed by positive skin tests and scaled fro...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2012.26.3750
更新日期:2012-01-01 00:00:00
abstract:PURPOSE:To evaluate the role of allergen immunotherapy (AIT) in the treatment of allergic rhinitis (AR). METHOD:Individual studies, systematic reviews, and practice guidelines that included information on sublingual AIT (SLIT) and subcutaneous AIT (SCIT) in the treatment of AR were considered. RESULTS:AIT via the SLI...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章,评审
doi:10.2500/ajra.2016.30.4253
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:A number of studies suggested that limited economical airborne allergy screening can successfully predict true respiratory allergy and be of use to primary care providers (PCPs) in improving the accuracy of their differential diagnosis and treatment of allergic rhinitis. More accurate diagnosis would lead to...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2012.26.3764
更新日期:2012-05-01 00:00:00
abstract:BACKGROUNDS:Diagnosis of allergic fungal rhinosinusitis (AFRS) is complicated because of the presence of fungi on mucosal surfaces of sinonasal passages. The objectives of this study were to define, using immunohistochemistry, lymphocyte populations associated with noninvasive fungal-related chronic rhinosinusitis (CRS...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2013.27.3882
更新日期:2013-05-01 00:00:00
abstract:BACKGROUND:Standardized allergen-specific immunotherapy (SIT) has been used in China for years. However, there is no extensive study of the safety of standardized SIT in Chinese patients until now. The aim of the current study is to perform a prospective and multicenter study to evaluate the systemic reactions (SRs) of...
journal_title:American journal of rhinology & allergy
pub_type: 临床试验,杂志文章,多中心研究
doi:10.2500/ajra.2014.28.4005
更新日期:2014-01-01 00:00:00
abstract:BACKGROUND:Conclusive evidence exists that biofilms are present on the mucosa of chronic rhinosinusitis (CRS) patients. Less is known about the species constituting these biofilms. This study developed a fluorescence in situ hybridization (FISH) protocol for characterization of bacterial and fungal biofilms in CRS. ME...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2009.23.3413
更新日期:2009-11-01 00:00:00
abstract::Background Since the Food and Drug Administration approval of balloon sinuplasty (BSP) in 2005, no nationally representative population-based studies have examined the outcomes and adverse events of BSP. Objective To assess the rates of complications and revision surgery in patients who received BSP, functional endosc...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.1177/1945892418782248
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:Chronic rhinosinusitis with nasal polyps (CRSwNPs) is the most challenging form of CRS to treat. Clinical practices vary widely among physicians around the world, often with little evidence to support current therapies. METHODS:This study evaluates various medical and surgical treatments with a focus on the...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2013.27.3982
更新日期:2013-11-01 00:00:00
abstract:BACKGROUND:Liquid-based cytology (LBC) has achieved great success in cytological diagnosis of various cancers when compared with conventional smear methods. However, its application in diagnosing nasopharyngeal carcinoma (NPC) has never been studied. METHODS:Eighty-four consecutive patients who underwent nasopharyngea...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2009.23.3341
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:The technical challenges of endoscopic sinus surgery (ESS) and the high risk of complications support the development of alternative modalities to train residents in these procedures. Virtual reality simulation is becoming a useful tool for training the skills necessary for minimally invasive surgery; howeve...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2014.28.4046
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Psoriasin is known to be expressed in diverse organs, where it exerts antimicrobial activity. Psoriasin is also involved in the local host defense mechanism against pathogens. We hypothesized that allergy-related T-helper cell type 2 (Th2) cytokines may regulate the expression of psoriasin. METHODS:We treat...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2014.28.4087
更新日期:2014-11-01 00:00:00
abstract:BACKGROUND:Allergic rhinitis (AR) is currently the most prevalent allergic disease in children and adolescents. OBJECTIVE:Surveys conducted by population-based studies of East Asia revealed an increased prevalence of behavioral disorders in patients with AR. Thus, in this study, we explored the prevalence of attention...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2017.31.4439
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:Allergic rhinitis is commonly treated with antihistamines. Monitoring improvement of airway inflammation noninvasively using nasal nitric oxide (nNO) would be clinically useful. To determine the anti-inflammatory effect of oral levocetirizine dihydrochloride (LC), we measured nNO and nasal eosinophils (nEos...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章,随机对照试验
doi:10.2500/ajra.2011.25.3668
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Assessing surgical competency in otolaryngology is challenging, and residency programs are now responsible for ensuring the surgical competency of their graduates. Therefore, more objective assessment tools are being incorporated into the evaluation process. Objective structured assessment of technical skill...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2014.28.4051
更新日期:2014-07-01 00:00:00
abstract:BACKGROUND:Nasal septal deviation (NSD) is a very common deformity. Because it may induce chronic upper airway obstruction in the nose, clinicians encounter some patients who have concerns about whether this can be a risk factor for general health. However, the influence of NSD on general health has rarely been studied...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2016.30.4277
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Biofilm formation has been implicated as an etiologic factor in the development of chronic rhinosinusitis (CRS). Nasal irrigation with surfactants including dilute baby shampoo have been proposed as an antibiofilm treatment for CRS patients. The effect of dilute baby shampoo on normal sinonasal mucosal funct...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2011.25.3583
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:The goal of endoscopic sinus surgery in chronic sinusitis is to create good drainage of the paranasal sinuses. Metal stents used to prevent lateralization of the middle concha are associated with adverse effects. OBJECTIVE:The aim of this experimental study was to evaluate the feasibility and safety of a ne...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2017.31.4401
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:The management of allergic rhinitis (AR) encompasses education, pharmacotherapy, immunotherapy, and surgery. FK506 (tacrolimus) is an immunosuppressant that inhibits allergic reactions. The purpose of this study was to reveal whether FK506 treatment reduces allergic inflammation in an AR mouse model and to ...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2012.26.3743
更新日期:2012-03-01 00:00:00
abstract:BACKGROUND:Skin prick test (SPT) or Phadiatop, a multi-allergen IgE screening test, was used as a tool for detecting aeroallergen sensitization. OBJECTIVE:To compare SPT and Phadiatop as a tool for diagnosis allergic rhinitis (AR) using the nasal provocation test (NPT) as a comparative standard. METHODS:Children aged...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.1177/1945892420938300
更新日期:2021-01-01 00:00:00
abstract::Chronic rhinosinusitis (CRS) affects 12.5% of the U.S. POPULATION:CRS can be divided into CRS with nasal polyps (CRSwNP) and CRS without nasal polyps. Some individuals with CRSwNP do not respond to standard-of-care medical and surgical management. For these individuals, targeted biologic agents are emerging as an imp...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章,评审
doi:10.2500/ajra.2017.31.4412
更新日期:2017-03-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to investigate the expression and distribution of superoxide anion, NADPH oxidase (NOX)1, and NOX4 in healthy, allergic nasal mucosa and nasal polyps to evaluate the possible influence of oxidative stress on the development of allergic rhinitis and nasal polyps. METHODS:The exp...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2009.23.3340
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:CD203c is a basophil surface marker and its expression is rapidly up-regulated after cross-linking of high-affinity immunoglobulin E (IgE) receptor (FcepsilonR1) by an allergen. CD203c basophil activation tests have been studied for the in vitro diagnosis of several allergic conditions. However, there is li...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2011.25.3723
更新日期:2011-11-01 00:00:00
abstract:BACKGROUND:Gelsolin is an actin-binding protein with multiple cellular functions including apoptosis and is reported to be down-regulated in various cancers and premalignant lesions. The objective of this study was to identify gelsolin and caspase-3 expression in inverted papilloma (IP) and investigate the role of gels...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2012.26.3753
更新日期:2012-05-01 00:00:00
abstract:BACKGROUND:The ciliary epithelial cells in the paranasal sinuses produce nasal nitric oxide (NO) continuously and plays a variety of roles in the paranasal sinuses. The purpose of this study was to assess whether we can use the levels of nasal NO (nNO) and exhaled NO (eNO) as a tool for evaluation in chronic rhinosinus...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2014.28.3984
更新日期:2014-01-01 00:00:00
abstract::Background Retronasal olfaction is important in flavor detection and enjoyment. The ability to identify specific individual retronasal odors may play a role in quality of life for patients with chronic rhinosinusitis (CRS). Objective To identify patterns and improve understanding of retronasal identification of indivi...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.1177/1945892418793540
更新日期:2018-09-01 00:00:00
abstract:BACKGROUND:Chronic rhinosinusitis (CRS) has a multifactorial etiology, with a debate about the role of inhalant allergy in the pathogenesis of CRS. OBJECTIVE:This study assessed the impact of allergy status on externalized paranasal sinuses after tumor resection to determine if a predisposition to inhalant allergy bro...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2017.31.4388
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Epiphora has a significant impact on the patient's quality of life and is commonly caused by nasolacrimal duct obstruction. Dacryocystorhinostomy (DCR) has traditionally been performed via an external approach, which involves a skin incision. With the advent of endoscopes, the endonasal approach to DCR has g...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.2500/ajra.2018.32.4494
更新日期:2018-01-01 00:00:00
abstract:BACKGROUND:Patients report nasal congestion as the most bothersome seasonal allergic rhinitis (SAR) symptom. Measurement of this symptom in previous research has largely been based on subjective patient ratings. This study was designed to measure efficacy, onset, and duration of action of the corticosteroid mometasone ...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章,随机对照试验
doi:10.2500/ajra.2010.24.3548
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND:Sex hormones are known to have some influence on nasal functions, but their effect on the decongestive response of the nasal mucosa during menstrual cycle is still undetermined. OBJECTIVES:The aim of this study was to examine the nasal physiology, the interconnectedness of olfactory and respiratory nasal fu...
journal_title:American journal of rhinology & allergy
pub_type: 杂志文章
doi:10.1177/1945892421990308
更新日期:2021-01-26 00:00:00