当前位置： SCI文献检索 > Cell Host & Microbe期刊下所有文献 > Spacer Acquisition Rates Determine the Immunological Diversity of the Type II CRISPR-Cas Immune Response.

Spacer Acquisition Rates Determine the Immunological Diversity of the Type II CRISPR-Cas Immune Response.

Abstract：

:CRISPR-Cas systems provide acquired immunity in prokaryotes. Upon infection, short sequences from the phage genome, known as spacers, are inserted between the CRISPR repeats. Spacers are transcribed into small RNA molecules that guide nucleases to their targets. The forces that shape the distribution of newly acquired spacers, which is observed to be uneven, are poorly understood. We studied the spacer patterns that arise after phage infection of Staphylococcus aureus harboring the Streptococcus pyogenes type II-A CRISPR-Cas system. We observed that spacer patterns are established early during the CRISPR-Cas immune response and correlate with spacer acquisition rates, but not with spacer targeting efficiency. The rate of spacer acquisition depended on sequence elements within the spacer, which in turn determined the abundance of different spacers within the adapted population. Our results reveal how the two main forces of the CRISPR-Cas immune response, acquisition and targeting, affect the generation of immunological diversity.

journal_name

Cell Host Microbe

journal_title

Cell host & microbe

authors

Heler R,Wright AV,Vucelja M,Doudna JA,Marraffini LA

doi

10.1016/j.chom.2018.12.016

subject

Has Abstract

pub_date

2019-02-13 00:00:00

pages

242-249.e3

issue

2

eissn

1931-3128

issn

1934-6069

pii

S1931-3128(18)30643-7

journal_volume

25

pub_type

杂志文章

相关文献

Cell Host & Microbe文献大全
  • A Knockout Screen of ApiAP2 Genes Reveals Networks of Interacting Transcriptional Regulators Controlling the Plasmodium Life Cycle.

    abstract：:A family of apicomplexa-specific proteins containing AP2 DNA-binding domains (ApiAP2s) was identified in malaria parasites. This family includes sequence-specific transcription factors that are key regulators of development. However, functions for the majority of ApiAP2 genes remain unknown. Here, a systematic knockou...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2016.12.003

    authors： Modrzynska K,Pfander C,Chappell L,Yu L,Suarez C,Dundas K,Gomes AR,Goulding D,Rayner JC,Choudhary J,Billker O

    更新日期：2017-01-11 00:00:00

  • Reciprocal inhibition between intracellular antiviral signaling and the RNAi machinery in mammalian cells.

    abstract：:RNA interference (RNAi) is an established antiviral defense mechanism in plants and invertebrates. Whether RNAi serves a similar function in mammalian cells remains unresolved. We find that in some cell types, mammalian RNAi activity is reduced shortly after viral infection via poly-ADP-ribosylation of the RNA-induced...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2013.09.002

    authors： Seo GJ,Kincaid RP,Phanaksri T,Burke JM,Pare JM,Cox JE,Hsiang TY,Krug RM,Sullivan CS

    更新日期：2013-10-16 00:00:00

  • All for one and one for all: herpesviral microRNAs close in on their prey.

    abstract：:Herpesviruses subvert immune cell activation by inhibiting NK cell receptor (NKG2D)-activating ligands such as MICB. A human cytomegalovirus microRNA was recently shown to repress MICB expression. Nachmani et al. (2009) extend this finding to two other human herpesviruses, providing evidence for a conserved functional...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2009.03.009

    authors： Dölken L,Jonjic S

    更新日期：2009-04-23 00:00:00

  • Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production.

    abstract：:The HIV-1 accessory protein Vpr enhances infection of primary macrophages through unknown mechanisms. Recent studies demonstrated that Vpr interactions with the cellular DCAF1-DDB1-CUL4 E3 ubiquitin ligase complex limit activation of innate immunity and interferon (IFN) induction. We describe a restriction mechanism t...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2014.10.014

    authors： Mashiba M,Collins DR,Terry VH,Collins KL

    更新日期：2014-12-10 00:00:00

  • The Spatial Heterogeneity of the Gut Limits Predation and Fosters Coexistence of Bacteria and Bacteriophages.

    abstract：:The ecological dynamics underlying the coexistence between antagonistic populations of bacteria and their viruses, bacteriophages (phages), in the mammalian gut microbiota remain poorly understood. We challenged a murine synthetic bacterial community with phages to study the factors allowing phages-bacteria coexistenc...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2020.06.002

    authors： Lourenço M,Chaffringeon L,Lamy-Besnier Q,Pédron T,Campagne P,Eberl C,Bérard M,Stecher B,Debarbieux L,De Sordi L

    更新日期：2020-09-09 00:00:00

  • Fungal Mimicry of a Mammalian Aminopeptidase Disables Innate Immunity and Promotes Pathogenicity.

    abstract：:Systemic fungal infections trigger marked immune-regulatory disturbances, but the mechanisms are poorly understood. We report that the pathogenic yeast of Blastomyces dermatitidis elaborates dipeptidyl-peptidase IVA (DppIVA), a close mimic of the mammalian ectopeptidase CD26, which modulates critical aspects of hemato...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2016.02.001

    authors： Sterkel AK,Lorenzini JL,Fites JS,Subramanian Vignesh K,Sullivan TD,Wuthrich M,Brandhorst T,Hernandez-Santos N,Deepe GS Jr,Klein BS

    更新日期：2016-03-09 00:00:00

  • HIV Latency: Stochastic across Multiple Scales.

    abstract：:Proviral latency is a major barrier to a cure for HIV. In this issue of Cell Host & Microbe, Hataye et al. (2019) show that reactivation of HIV latency is a non-deterministic, highly stochastic (i.e., noisy) process and propose that stochastic transitions to exponential viral expansion require a critical threshold of ...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2019.11.010

    authors： Hansen MMK,Martin B,Weinberger LS

    更新日期：2019-12-11 00:00:00

  • Hepatitis C virus blocks interferon effector function by inducing protein kinase R phosphorylation.

    abstract：:Hepatitis C virus (HCV) is a single-stranded RNA virus encoding a single polyprotein whose translation is driven by an internal ribosome entry site (IRES). HCV infection strongly induces antiviral interferon-stimulated gene (ISG) expression in the liver, yet it persists, suggesting that HCV can block ISG effector func...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2009.11.004

    authors： Garaigorta U,Chisari FV

    更新日期：2009-12-17 00:00:00

  • Commensal Bacteroides species induce colitis in host-genotype-specific fashion in a mouse model of inflammatory bowel disease.

    abstract：:The intestinal microbiota is important for induction of inflammatory bowel disease (IBD). IBD is associated with complex shifts in microbiota composition, but it is unclear whether specific bacterial subsets induce IBD and, if so, whether their proportions in the microbiota are altered during disease. Here, we fulfill...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2011.04.009

    authors： Bloom SM,Bijanki VN,Nava GM,Sun L,Malvin NP,Donermeyer DL,Dunne WM Jr,Allen PM,Stappenbeck TS

    更新日期：2011-05-19 00:00:00

  • Group A Streptococcal M1 Protein Sequesters Cathelicidin to Evade Innate Immune Killing.

    abstract：:The antimicrobial peptide LL-37 is generated upon proteolytic cleavage of cathelicidin and limits invading pathogens by directly targeting microbial membranes as well as stimulating innate immune cell function. However, some microbes evade LL-37-mediated defense. Notably, group A Streptococcus (GAS) strains belonging ...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2015.09.004

    authors： LaRock CN,Döhrmann S,Todd J,Corriden R,Olson J,Johannssen T,Lepenies B,Gallo RL,Ghosh P,Nizet V

    更新日期：2015-10-14 00:00:00

  • Structural basis for signaling by exclusive EDS1 heteromeric complexes with SAG101 or PAD4 in plant innate immunity.

    abstract：:Biotrophic plant pathogens encounter a postinfection basal resistance layer controlled by the lipase-like protein enhanced disease susceptibility 1 (EDS1) and its sequence-related interaction partners, senescence-associated gene 101 (SAG101) and phytoalexin deficient 4 (PAD4). Maintainance of separate EDS1 family memb...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2013.11.006

    authors： Wagner S,Stuttmann J,Rietz S,Guerois R,Brunstein E,Bautor J,Niefind K,Parker JE

    更新日期：2013-12-11 00:00:00

  • Got Lactobacillus? Commensals Power Growth.

    abstract：:Although Lactobacilli are generally considered probiotic agents in metazoans, the underlying molecular mechanisms are largely unknown. In this issue of Cell Host & Microbe, Erkosar et al. (2015) reveal that a Drosophila gut commensal, Lactobacillus plantarum(WJL), promotes animal growth by enhancing the host's capacit...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2015.09.012

    authors： Park DY,Lee WJ,Jang IH,Lee WJ

    更新日期：2015-10-14 00:00:00

  • The oral commensal microbiota bites back through Nod1.

    abstract：:The mechanisms through which commensal bacterial populations cause inflammatory disease when shifted to dysbiotic community structures are poorly understood. Jiao et al. (2013) demonstrate that, in the case of inflammatory disease in the mouth, stimulation of the intracellular pattern recognition receptor Nod1 is a cr...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2013.05.001

    authors： Curtis M

    更新日期：2013-05-15 00:00:00

  • Proteome-wide quantification and characterization of oxidation-sensitive cysteines in pathogenic bacteria.

    abstract：:Thiol-group oxidation of active and allosteric cysteines is a widespread regulatory posttranslational protein modification. Pathogenic bacteria, including Pseudomonas aeruginosa and Staphylococcus aureus, use regulatory cysteine oxidation to respond to and overcome reactive oxygen species (ROS) encountered in the host...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2013.02.004

    authors： Deng X,Weerapana E,Ulanovskaya O,Sun F,Liang H,Ji Q,Ye Y,Fu Y,Zhou L,Li J,Zhang H,Wang C,Alvarez S,Hicks LM,Lan L,Wu M,Cravatt BF,He C

    更新日期：2013-03-13 00:00:00

  • Fiber-Mediated Nourishment of Gut Microbiota Protects against Diet-Induced Obesity by Restoring IL-22-Mediated Colonic Health.

    abstract：:Dietary supplementation with fermentable fiber suppresses adiposity and the associated parameters of metabolic syndrome. Microbiota-generated fiber-derived short-chain fatty acids (SCFAs) and free fatty acid receptors including GPR43 are thought to mediate these effects. We find that while fermentable (inulin), but no...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2017.11.003

    authors： Zou J,Chassaing B,Singh V,Pellizzon M,Ricci M,Fythe MD,Kumar MV,Gewirtz AT

    更新日期：2018-01-10 00:00:00

  • The relationship of the oral microbiotia to periodontal health and disease.

    abstract：:The oral microbial community represents the best-characterized consortium associated with the human host. There are strong correlations between the qualitative composition of the oral microbiota and clinically healthy or diseased states. However, additional studies are needed to elucidate the mechanisms that define th...

    journal_title：Cell host & microbe

    pub_type： 杂志文章,评审

    doi：10.1016/j.chom.2011.09.008

    authors： Curtis MA,Zenobia C,Darveau RP

    更新日期：2011-10-20 00:00:00

  • Shigella phagocytic vacuolar membrane remnants participate in the cellular response to pathogen invasion and are regulated by autophagy.

    abstract：:Intracellular pathogens like Shigella flexneri enter host cells by phagocytosis. Once inside, the pathogen breaks the vacuolar membrane for cytosolic access. The fate and function of the vacuolar membrane remnants are not clear. Examining Shigella-infected nonmyeloid cells, we observed that proteins associated with va...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2009.07.005

    authors： Dupont N,Lacas-Gervais S,Bertout J,Paz I,Freche B,Van Nhieu GT,van der Goot FG,Sansonetti PJ,Lafont F

    更新日期：2009-08-20 00:00:00

  • Tousled-like kinases modulate reactivation of gammaherpesviruses from latency.

    abstract：:Kaposi's sarcoma-associated herpesvirus (KSHV) is linked to human malignancies. The majority of tumor cells harbor latent virus, and a small percentage undergo spontaneous lytic replication. Both latency and lytic replication are important for viral pathogenesis and spread, but the cellular players involved in the swi...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2012.12.005

    authors： Dillon PJ,Gregory SM,Tamburro K,Sanders MK,Johnson GL,Raab-Traub N,Dittmer DP,Damania B

    更新日期：2013-02-13 00:00:00

  • Cholesterol Metabolism by Uncultured Human Gut Bacteria Influences Host Cholesterol Level.

    abstract：:The human microbiome encodes extensive metabolic capabilities, but our understanding of the mechanisms linking gut microbes to human metabolism remains limited. Here, we focus on the conversion of cholesterol to the poorly absorbed sterol coprostanol by the gut microbiota to develop a framework for the identification ...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2020.05.013

    authors： Kenny DJ,Plichta DR,Shungin D,Koppel N,Hall AB,Fu B,Vasan RS,Shaw SY,Vlamakis H,Balskus EP,Xavier RJ

    更新日期：2020-08-12 00:00:00

  • Viperin Poisons Viral Replication.

    abstract：:Control of virus infection relies on the stimulation of interferon-stimulated genes (ISGs) that inhibit viral replication. In a recent Nature paper, Gizzi et al. (2018) discovered that the ISG viperin inhibits virus replication by generating the ribonucleotide ddhCTP, which interferes with RNA synthesis, thus offering...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2018.07.014

    authors： Ng LFP,Hiscox JA

    更新日期：2018-08-08 00:00:00

  • KREMEN1 Is a Host Entry Receptor for a Major Group of Enteroviruses.

    abstract：:Human type A Enteroviruses (EV-As) cause diseases ranging from hand-foot-and-mouth disease to poliomyelitis-like disease. Although cellular receptors are identified for some EV-As, they remain elusive for the majority of EV-As. We identify the cell surface molecule KREMEN1 as an entry receptor for coxsackievirus A10 (...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2018.03.019

    authors： Staring J,van den Hengel LG,Raaben M,Blomen VA,Carette JE,Brummelkamp TR

    更新日期：2018-05-09 00:00:00

  • Impaired stimulation of p38α-MAPK/Vps41-HOPS by LPS from pathogenic Coxiella burnetii prevents trafficking to microbicidal phagolysosomes.

    abstract：:Variations in lipopolysaccharide (LPS), a bacterial outer membrane component, determine virulence of the obligate intracellular bacterium Coxiella burnetii, but the underlying mechanisms are unknown. We find that while avirulent C. burnetii LPS (avLPS) stimulates host p38α-MAPK signaling required for proper traffickin...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2012.10.015

    authors： Barry AO,Boucherit N,Mottola G,Vadovic P,Trouplin V,Soubeyran P,Capo C,Bonatti S,Nebreda A,Toman R,Lemichez E,Mege JL,Ghigo E

    更新日期：2012-12-13 00:00:00

  • Induction of bone loss by pathobiont-mediated Nod1 signaling in the oral cavity.

    abstract：:Periodontitis is a common disease that is characterized by resorption of the alveolar bone and mediated by commensal bacteria that trigger host immune responses and bone destruction through unidentified mechanisms. We report that Nod1, an innate intracellular host receptor for bacterial peptidoglycan-related molecules...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2013.04.005

    authors： Jiao Y,Darzi Y,Tawaratsumida K,Marchesan JT,Hasegawa M,Moon H,Chen GY,Núñez G,Giannobile WV,Raes J,Inohara N

    更新日期：2013-05-15 00:00:00

  • Fragment and Conquer.

    abstract：:The replication vacuole of Legionella pneumophila makes contact with host mitochondria. In this issue of Cell Host & Microbe, Escoll et al. (2017) dissect the mechanisms of this interaction, the effect of the T4SS effector MitF on mitochondrial function, and the resultant metabolic reprogramming of infected cells to b...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2017.08.014

    authors： Kozjak-Pavlovic V,Chowdhury SR,Rudel T

    更新日期：2017-09-13 00:00:00

  • Plasmodium protein export at higher PEXEL resolution.

    abstract：:Intraerythrocytic malaria parasites send hundreds of effector proteins into the host cell. Diverse modes of export have been proposed for different proteins. In this issue, Grüring et al. (2012) present findings that bring the models together. ...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2012.11.001

    authors： Goldberg DE

    更新日期：2012-11-15 00:00:00

  • Tracking Vibrio cholerae Cell-Cell Interactions during Infection Reveals Bacterial Population Dynamics within Intestinal Microenvironments.

    abstract：:Vibrio cholerae is the causative agent of the diarrheal disease cholera. Although many V. cholerae virulence factors have been studied, the role of interbacterial interactions within the host gut and their influence on colonization are poorly understood. Here, we utilized the conjugative properties of a Vibrio-specifi...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2017.12.006

    authors： Fu Y,Ho BT,Mekalanos JJ

    更新日期：2018-02-14 00:00:00

  • Exploiting Combinatorial Interactions to Expand NLR Specificity.

    abstract：:Intracellular nucleotide-binding leucine-rich repeat (NLR) receptors play central roles in human and plant innate immunity. In this issue of Cell Host & Microbe, Wang et al. (2015) show that a single plant NLR can detect diverse pathogen effectors by partnering with different scaffolding proteins, which can each recog...

    journal_title：Cell host & microbe

    pub_type： 评论,杂志文章

    doi：10.1016/j.chom.2015.08.011

    authors： Innes RW

    更新日期：2015-09-09 00:00:00

  • Both Neutralizing and Non-Neutralizing Human H7N9 Influenza Vaccine-Induced Monoclonal Antibodies Confer Protection.

    abstract：:Pathogenic H7N9 avian influenza viruses continue to represent a public health concern, and several candidate vaccines are currently being developed. It is vital to assess if protective antibodies are induced following vaccination and to characterize the diversity of epitopes targeted. Here we characterized the binding...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2016.05.014

    authors： Henry Dunand CJ,Leon PE,Huang M,Choi A,Chromikova V,Ho IY,Tan GS,Cruz J,Hirsh A,Zheng NY,Mullarkey CE,Ennis FA,Terajima M,Treanor JJ,Topham DJ,Subbarao K,Palese P,Krammer F,Wilson PC

    更新日期：2016-06-08 00:00:00

  • Salmonella Typhoid Toxin PltB Subunit and Its Non-typhoidal Salmonella Ortholog Confer Differential Host Adaptation and Virulence.

    abstract：:Typhoidal and non-typhoidal Salmonelleae (NTS) cause typhoid fever and gastroenteritis, respectively, in humans. Salmonella typhoid toxin contributes to typhoid disease progression and chronic infection, but little is known about the role of its NTS ortholog. We found that typhoid toxin and its NTS ortholog induce dif...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2020.04.005

    authors： Lee S,Yang YA,Milano SK,Nguyen T,Ahn C,Sim JH,Thompson AJ,Hillpot EC,Yoo G,Paulson JC,Song J

    更新日期：2020-06-10 00:00:00

  • Type I Interferon Response Dysregulates Host Iron Homeostasis and Enhances Candida glabrata Infection.

    abstract：:Type I interferons (IFNs-I) fulfil multiple protective functions during pathogenic infections, but they can also cause detrimental effects and enhance immunopathology. Here, we report that IFNs-I promote the dysregulation of iron homeostasis in macrophages during systemic infections with the intracellular pathogen Can...

    journal_title：Cell host & microbe

    pub_type： 杂志文章

    doi：10.1016/j.chom.2020.01.023

    authors： Riedelberger M,Penninger P,Tscherner M,Seifert M,Jenull S,Brunnhofer C,Scheidl B,Tsymala I,Bourgeois C,Petryshyn A,Glaser W,Limbeck A,Strobl B,Weiss G,Kuchler K

    更新日期：2020-03-11 00:00:00