Abstract：
:Exosomes are cell-derived vesicles that are secreted by many eukaryotic cells. It has recently attracted attention as vehicles of intercellular communication. Virus-infected cells release exosomes, which contain viral proteins, RNA, and pathogenic molecules. However, the role of exosomes in virus infection process remains unclear and needs to be further investigated. In this study, we aimed to evaluate the effects of exosomes on rabies virus infection. OptiPrep™ density gradient centrifugation was used to isolate exosomes from rabies virus-infected cell culture supernatants. A rabies virus G protein enzyme-linked immunosorbent assay and acetylcholinesterase activity assays were performed to verify the centrifugation fractions. Exosomes were then characterized using transmission electron microscopy and Western blotting. Our results showed that rabies virus infection increased the release of exosomes. Treatment with GW4869 and si-Rab27a, two exosomal secretion inhibitors, inhibited exosome release. Furthermore, the inhibitors reduced the levels of extracellular and intracellular viral RNA. These data indicated that exosomes may participate in the viral infection process. Moreover, our results establish a basis for future research into the roles of exosomes in rabies virus infection and as potential targets for developing new antiviral strategies.
journal_name
Virol Sinjournal_title
Virologica Sinicaauthors
Wang J,Wu F,Liu C,Dai W,Teng Y,Su W,Kong W,Gao F,Cai L,Hou A,Jiang Cdoi
10.1007/s12250-019-00087-3subject
Has Abstractpub_date
2019-02-01 00:00:00pages
59-65issue
1eissn
1674-0769issn
1995-820Xpii
10.1007/s12250-019-00087-3journal_volume
34pub_type
杂志文章abstract：:Human cytomegalovirus (HCMV), a herpesvirus, is an important human pathogen that causes asymptomatic infections in healthy or immunocompetent individuals but can lead to severe and potentially life-threatening complications in immune-immature individuals such as neonates or immune-compromised patients such as organ-tr...
journal_title：Virologica Sinica
pub_type： 杂志文章,评审
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abstract：:Hepatitis B virus (HBV) infection is still a worldwide health problem; however, the current antiviral therapies for chronic hepatitis B are limited in efficacy. The outcome of HBV infection is thought to be the result of complex interactions between the HBV and the host immune system. While the role of the adaptive im...
journal_title：Virologica Sinica
pub_type： 杂志文章,评审
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journal_title：Virologica Sinica
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journal_title：Virologica Sinica
pub_type： 杂志文章,评审
doi：10.1007/s12250-010-3136-y
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journal_title：Virologica Sinica
pub_type： 杂志文章,meta分析
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journal_title：Virologica Sinica
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更新日期：2010-02-01 00:00:00
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journal_title：Virologica Sinica
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journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-019-00134-z
更新日期：2019-10-01 00:00:00
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journal_title：Virologica Sinica
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更新日期：2017-02-01 00:00:00
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journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-019-00174-5
更新日期：2020-02-01 00:00:00
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journal_title：Virologica Sinica
pub_type： 杂志文章
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journal_title：Virologica Sinica
pub_type： 杂志文章,评审
doi：10.1007/s12250-016-3726-4
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journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-017-4077-5
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journal_title：Virologica Sinica
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journal_title：Virologica Sinica
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journal_title：Virologica Sinica
pub_type： 杂志文章
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journal_title：Virologica Sinica
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journal_title：Virologica Sinica
pub_type： 杂志文章
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journal_title：Virologica Sinica
pub_type： 杂志文章,评审
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更新日期：2020-09-01 00:00:00
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journal_title：Virologica Sinica
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journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-012-3284-3
更新日期：2012-12-01 00:00:00
abstract：:The epidemiology of HIV-1 varies in different areas of the world, and it is possible that this complexity may leave unique footprints in the viral genome. Thus, we attempted to find significant patterns in global HIV-1 genome sequences. By applying the rule inference algorithm RIPPER (Repeated Incremental Pruning to P...
journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-013-3348-z
更新日期：2013-08-01 00:00:00
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journal_title：Virologica Sinica
pub_type： 杂志文章
doi：10.1007/s12250-020-00317-z
更新日期：2020-12-01 00:00:00
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journal_title：Virologica Sinica
pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
