Modeling traumatic injury in organotypic spinal cord slice culture obtained from adult rat.

Abstract:

:Nowadays there are various models of spinal cord injury (SCI) that recreate mechanisms of human SCI. The ex vivo modeling of injury is a robust approach, confronts with less experimental and ethical challenges. Currently almost all ex vivo models are obtained either from embryonic or postnatal animals, which can hardly mimic features of human SCI. This study was designed to develop SCI in slice culture of adult rats. Here, the lumbar enlargement of an adult rat was sliced and cultured. After seven days in vitro, a weight was dropped to simulate the injury. The result showed that although the rate of cell death in first days of in vitro was high, it reduced after 7 days and dropping a weight at the time caused significant rate of cell death in slices. It was shown that injury can disturb histological features and neuronal integrity in the slices. Treating the injured slices with valproic acid resulted in a significant decrease of TNF-α and increase of BDNF expression. Collected data revealed obtained slices from adult rat were able to adjust to the culture environment after 7 days and dropping a weight at the time point could simulate the injury. Besides mimicking the disturbing features of human SCI, this model can response to VPA pharmacological treatment.

journal_name

Tissue Cell

journal_title

Tissue & cell

authors

Pandamooz S,Salehi MS,Zibaii MI,Safari A,Nabiuni M,Ahmadiani A,Dargahi L

doi

10.1016/j.tice.2019.01.002

subject

Has Abstract

pub_date

2019-02-01 00:00:00

pages

90-97

eissn

0040-8166

issn

1532-3072

pii

S0040-8166(18)30357-4

journal_volume

56

pub_type

杂志文章
  • The influence of the donor on dental apical papilla stem cell properties.

    abstract::Stem cells from the human dental apical papilla (SCAP) can be obtained from almost all extracted wisdom teeth with an immature tooth root. Although different stem cell lines are used for studies, it remains elusive whether specific characteristics of the dental stem cell cultures such as proliferation rates or the cel...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2015.05.002

    authors: Prateeptongkum E,Klingelhöffer C,Morsczeck C

    更新日期:2015-08-01 00:00:00

  • Receptor-mediated endocytosis of a chemoreceptor involved in triggering the discharge of cnidae in a sea anemone tentacle.

    abstract::Collodial gold coated with the glycoprotein, bovine submaxillary mucin (BSM-gold), was used to localize chemoreceptors known to be involved in triggering the discharge of cnidae in sea anemones. BSM-gold binds exclusively at the apical surface of the supporting cell, the cell adjacent to the cnidocyte (Watson and Hess...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(87)90016-4

    authors: Watson GM,Hessinger DA

    更新日期:1987-01-01 00:00:00

  • Ultrastructural changes of bovine pulmonary artery endothelial cells irradiated in vitro with a 137Cs source.

    abstract::Bovine pulmonary artery endothelial cells in culture were evaluated by phase-contrast and electron microscopy at various times after being irradiated with 137Cs in vitro. Cells irradiated prior to reaching confluency showed vacuolization aand increased numbers of lysosomes beginning at 48 hr after irradiation with 300...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(83)90016-2

    authors: Lee SL,Douglas WH,Lin PS,Fanburg BL

    更新日期:1983-01-01 00:00:00

  • The polycystic ovarian (PCO) condition: apoptosis and epithelialization of the ovarian antral follicles are aspects of cystogenesis in the dehydroepiandrosterone (DHEA)-treated rat model.

    abstract::This investigation was designed to study apoptosis and epithelialization during cystogenesis of the dehydroepiandrosterone rat model. Using in situ DNA 3'- end-labeling with non-radioactive digoxigenindidesoxy-UTP (dig-ddUTP), apoptosis is initially seen in cumulus granulosa cells and other granulosa cells facing the ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(97)80017-1

    authors: Anderson E,Lee GY

    更新日期:1997-04-01 00:00:00

  • Ultrastructure of peritubular tissue in association with tubular hyalinization in human testis.

    abstract::Testicular peritubular tissue, also known as the tunica propria, surrounds the seminiferous tubules and is responsible for contractile, paracrine and transport functions. The aim of the present report is to describe the pathomorphology of peritubular tissue in association with tubular hyalinization in human testis. Tw...

    journal_title:Tissue & cell

    pub_type: 临床试验,杂志文章

    doi:10.1054/tice.1999.0005

    authors: Haider SG,Talati J,Servos G

    更新日期:1999-02-01 00:00:00

  • Scanning electron microscopy of induced cell rounding of mouse adrenal cortex tumor cells in culture.

    abstract::The surface topologies of mouse adrenal cortex tumor cells of primary or clonal origin grown as monolayer cell cultures were observed by scanning electron microscopy following their exposure to substances that effect steroid release and/or cell rounding. ACTH induced cell rounding with a concomitant profuse developmen...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(77)90034-9

    authors: Cuprak LJ,Lammi CJ,Bayer RC

    更新日期:1977-01-01 00:00:00

  • Histochemical specificity of cholinesterases to phenylthioacetate in differentiated neural tissues of insects and teleosts.

    abstract::Housefly brain cholinesterase was histochemically demonstrated to hydrolyse phenylthioacetate at a very high rate, similar in distribution to that previously reported for acetylthiocholine. However, teleost neural retina cholinesterase would not hydrolyse the aromatic substrate, but the enzyme did cleave acetylthiocho...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(70)80028-3

    authors: Booth GM,Whitt GS

    更新日期:1970-01-01 00:00:00

  • Hatching envelope formation in shrimp (Sicyonia ingentis) ova: Origin and sequential exocytosis of cortical vesicles.

    abstract::The ova of Sicyonia ingentis lack cortical vesicles at the time of spawning. Within 30 min post-spawning, two populations of cortical vesicles are organized in the ooplasm which, during cortical vesicle exocytosis (cortical reaction), successively release two morphologically different exudates. The first type of corti...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(88)90035-3

    authors: Pillai MC,Clark WH Jr

    更新日期:1988-01-01 00:00:00

  • Structure of the subgenual organ in the green lacewing, Chrysoperla carnea.

    abstract::REM and TEM studies of the subgenual organ in Chrysoperla carnea (Neuroptera: Chrysopidae) show that it is composed of three scolopidia, each with one sensory, one scolopale and one cap cell. The distal part of the dendrite shows a cilium with a '9 + 0' structure. The cross-handing pattern of the ciliary root has a pe...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(94)90100-7

    authors: Devetak D,Pabst MA

    更新日期:1994-04-01 00:00:00

  • The structure and function of the smooth septate junction in a transporting epithelium: the Malpighian tubules of the New Zealand glow-worm Arachnocampa luminosa.

    abstract::Junctional complexes between the epithelial cells in the four distinct regions of the glow-worm Malpighian tubule were investigated by electron microscopy using thin sectioning, freeze-fracturing, osmotic disruption and tracer techniques. The lateral plasma membranes of all four cell types are joined by smooth septate...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(80)90011-7

    authors: Green LF,Bergquist PR,Bullivant S

    更新日期:1980-01-01 00:00:00

  • A morphological study on gills of the brown shrimp, Penaeus aztecus.

    abstract::The gills of Penaeus aztecus were examined by light and electron microscopy. They are dendrobranchiate, consisting of a central axis with biserially arranged branches that subdivide into bifurcating filaments. A septum divides the lumina of these structures into afferent and efferent channels. Hemolymph from the stern...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(78)90008-3

    authors: Foster CA,Howse HD

    更新日期:1978-01-01 00:00:00

  • Evidence that Müller cells can phagocytize egg-lecithin-coated silicone particles.

    abstract::This study is designed to better understand the function of the Müller cells of the retina through the use of silicon particles that had been injected into the vitreous body of the eye and via an analysis of the number of gap junctions associated with these cells. Following intraocular injection of a silicone oil mixt...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(93)90028-j

    authors: Nishizono H,Murata Y,Tanaka M,Soji T,Herbert DC

    更新日期:1993-01-01 00:00:00

  • Bilateral eyestalk ablation of the blue swimmer crab, Portunus pelagicus, produces hypertrophy of the androgenic gland and an increase of cells producing insulin-like androgenic gland hormone.

    abstract::The androgenic glands (AG) of male decapod crustaceans produce insulin-like androgenic gland (IAG) hormone that controls male sex differentiation, growth and behavior. Functions of the AG are inhibited by gonad-inhibiting hormone originating from X-organ-sinus gland complex in the eyestalk. The AG, and its interaction...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2010.07.003

    authors: Sroyraya M,Chotwiwatthanakun C,Stewart MJ,Soonklang N,Kornthong N,Phoungpetchara I,Hanna PJ,Sobhon P

    更新日期:2010-10-01 00:00:00

  • Size changes in single muscle fibers during fixation and embedding.

    abstract::During fixation of single muscles fibers with glutaraldehyde, the volume of the fiber shrinks 20%, recovers in rinse and osmium tetroxide to near normal volume and shrinks 20% again when staining with uranyl acetate. This suggest that osmotic properties of membranes may not have been completely lost during fixation, p...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(75)90013-0

    authors: Eisenberg BR,Mobley BA

    更新日期:1975-01-01 00:00:00

  • Partial development of the steroidogenic ultrastructural features in degenerative corpora lutea after a single injection of pituitary extract in the Western painted turtle (Chrysemys picta).

    abstract::Pituitary glands were removed from sexually mature female turtles (Chrysemys picta) and they were injected intraperitoneally (i.p.) into other mature females of the same species (experimental). In addition mature females of the same species received saline injection only (controls). Initially all the turtles used in t...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2006.02.001

    authors: Mahmoud IY,Ba-Omar T,Alkindi A

    更新日期:2006-06-01 00:00:00

  • Different expressions of connexin 43 and 32 in the fibroblasts of human dental pulp.

    abstract::The expression and localization of gap junctional proteins connexin (Cx) 26, 32, and 43 was examined in human dental pulp. Dental pulp tissues were obtained from human third molars immediately after extraction. Some pulp tissues were used for cell culture, and the rest for histological observations. Immunostaining for...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(02)00028-9

    authors: Ibuki N,Yamaoka Y,Sawa Y,Kawasaki T,Yoshida S

    更新日期:2002-06-01 00:00:00

  • Development of serous cutaneous glands in Scinax nasica (Anura, Hylidae): patterns of poison biosynthesis and maturation in comparison with larval glands in specimens of other families.

    abstract::We examined the development of serous (poison) cutaneous glands in larval and juvenile Scinax nasica (Hylidae) at the ultrastructural level. We describe the biosynthesis and maturation of the cutaneous poison in comparison with the corresponding processes in representatives of Discoglossidae, Leptodactylidae, Pelobati...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(03)00049-1

    authors: Terreni A,Nosi D,Greven H,Delfino G

    更新日期:2003-08-01 00:00:00

  • In vitro effects of juvenile hormone analog on wing disc morphogenesis under ecdysteroid treatment in the female-wingless bagworm moth Eumeta variegata (Insecta: Lepidoptera, Psychidae).

    abstract::Female adults of the bagworm moth, Eumeta variegata, lack wings completely, whereas male adults of this species have functional wings. We previously found that ecdysteroid induces apoptotic events in the female wing rudiment of E. variegata in vitro, whereas the male wing discs cultured with 20-hydroxyecdysone (20E) u...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2011.01.003

    authors: Niitsu S,Lobbia S,Kamito T

    更新日期:2011-06-01 00:00:00

  • Phagocytosis plays an important role in clearing dead cells caused by mono(2-ethylhexyl) phthalate administration.

    abstract::The role of phagocytosis in eliminating apoptotic spermatogenic cells caused by mono(2-ethylhexyl) phthalate (MEHP) was studied. Twenty-one-day-old C57Bl/6N male mice were given a single dose of 800 mg/kg MEHP in corn oil by oral gavage and sacrificed at 1, 3, 5, 7 and 9 days after initial exposure. At the same time, ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2007.04.002

    authors: Tay TW,Andriana BB,Ishii M,Choi EK,Zhu XB,Alam MS,Tsunekawa N,Kanai Y,Kurohmaru M

    更新日期:2007-08-01 00:00:00

  • The ultrastructure of the peculiar synspermia of some Dysderidae (Araneae, Arachnida).

    abstract::The present study reports on the ultrastructure features of spermatozoa and spermatogenesis of several species of Dysderidae (Dysdera crocata, Dysdera erythrina, Dysdera ninnii, Harpactea arguta, Harpactea piligera, Dasumia taeniifera). Dysderid spiders are known to possess a peculiar sperm transfer form known as syns...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2004.08.001

    authors: Michalik P,Dallai R,Giusti F,Alberti G

    更新日期:2004-12-01 00:00:00

  • Arm-specific telomere dynamics of each individual chromosome in induced pluripotent stem cells revealed by quantitative fluorescence in situ hybridization.

    abstract::We have reported that telomere fluorescence units (TFUs) of established induced pluripotent stem cells (iPSCs) derived from human amnion (hAM933) and fetal lung fibroblasts (MRC-5) were significantly longer than those of the parental cells, and that the telomere extension rates varied quite significantly among clones ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2014.08.005

    authors: Terai M,Izumiyama-Shimomura N,Aida J,Ishikawa N,Kuroiwa M,Arai T,Toyoda M,Nakamura K,Takubo K

    更新日期:2014-12-01 00:00:00

  • Immunocytochemical study of tubulin in the 9 + '1' sperm axoneme of a monogenean (Platyhelminthes), Pseudodactylogyrus sp.

    abstract::The spermatozoon of the monopisthocotylean monogenean Pseudodactylogyrus sp. (a gill parasite of eels) has a single axoneme showing a 9 + '1' pattern, a nucleus and a mitochondrion, but has no cortical microtubules. This species thus provides a very simple model for the study of tubulin in the 9 + '1' axonemes of the ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/s0040-8166(97)80045-6

    authors: Mollaret I,Justine JL

    更新日期:1997-12-01 00:00:00

  • Acute effects of rat growth hormone (GH), human GH and prolactin on proliferating rat liver cells in vitro: a study of mitotic behaviour and ultrastructural alterations.

    abstract::Examination of livers from transgenic mice over expressing human growth hormone (hGH) revealed numerous alterations including a striking incidence of mitotic figures. The reason for increased proliferation is unclear, but could be related to effects of hGH, which is also acting as a lactogen in rodents. In order to id...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(94)90029-9

    authors: Vergani G,Mayerhofer A,Bartke A

    更新日期:1994-06-01 00:00:00

  • Studies on the in vivo cellular reactions of insects: an ultrastructural analysis of nodule formation in Galleria mellonella.

    abstract::Cellular aggregates or nodules which formed in Galleria mellonella larvae in response to injections of killed Bacillus cereus were examined during their first 24 hr of development. The initial aggregation stage was very rapid, occurring within 1 min, and was brought about by the release of an adhesive flocculent mater...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(77)90050-7

    authors: Ratcliffe NA,Gagen SJ

    更新日期:1977-01-01 00:00:00

  • 'Twisted plywood' structure and mineralization in the scales of a primitive living fish Amia calva.

    abstract::The basal plate of the scales of Amia calva is composed of regular double twisted plywood, as in Latimeria and Dipnoan scales. However, the progressive rotation of the fibrils direction is left-handed in Amia and right-handed in the 'Sarcopterygians'. So, the similarity between these peculiar plywoods is probably the ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(81)90046-x

    authors: Meunier FJ

    更新日期:1981-01-01 00:00:00

  • Eye muscles of the pike (Esox lucius) fiber types and their actomyosin ATPase and SDH activity.

    abstract::The eye muscles of the pike are organized in two distinct populations of fibers arranged in semicircular layers. The distal layer, consisting of small diameter muscle fibers, is mainly made up of type I and tonic (slow) fibers. The central and proximal (facing the eyeball) parts of the eye muscles are occupied by IIB ...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(91)90021-k

    authors: Zawadowska B

    更新日期:1991-01-01 00:00:00

  • Chemotaxis of granulocytes across bovine pulmonary artery intimal explants without endothelial cell injury.

    abstract::Emigration of granulocytes from vessel lumen to a site of injury is a hallmark of acute inflammation but whether this migration is necessarily associated with vascular damage is not clear. To follow the structural changes associated with granulocyte migration across an intact endothelial cell layer and to assess chang...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(84)90014-4

    authors: Meyrick B,Hoffman LH,Brigham KL

    更新日期:1984-01-01 00:00:00

  • Expression of asprosin in rat hepatic, renal, heart, gastric, testicular and brain tissues and its changes in a streptozotocin-induced diabetes mellitus model.

    abstract::In this study, we aimed to investigate the presence of asprosin (ASP) in the liver, kidneys, heart, stomach, testicles and brain and to determine the serum and tissue asprosin levels in diabetic rats. A total of 14 male Wistar Albino rats were divided into two groups, each containing 7 rats: (I) control group and (II)...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/j.tice.2020.101397

    authors: Kocaman N,Kuloğlu T

    更新日期:2020-10-01 00:00:00

  • Cytokeratin filament modulation in pulmonary microvessel endothelial cells by vasoactive agents and culture confluency.

    abstract::Recently, bovine pulmonary microvascular endothelial cells (PMV) were shown to contain cytokeratin 8 and 19 intermediate filaments (Patton et al., 1990). In this study, we examine the effect of culture contiguity and vasoactive agents on the content and assembly of cytokeratins in PMV. Immunofluorescent staining of PM...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(91)90069-6

    authors: Alexander JS,Patton WF,Yoon MU,Shepro D

    更新日期:1991-01-01 00:00:00

  • Redistribution of epidermal growth factor receptor as a function of cell density, cell-cell adhesion and calcium in human (A-431) cells.

    abstract::Cell surface expression of the epidermal growth factor (EGF) receptor in several cell lines declines as a function of increased cell density and is associated with diminished responsiveness to EGF. However, the mechanism whereby this density-induced down regulation of receptors occurs has not been discerned. In the pr...

    journal_title:Tissue & cell

    pub_type: 杂志文章

    doi:10.1016/0040-8166(93)90061-o

    authors: Suárez-Quian CA,Byers SW

    更新日期:1993-01-01 00:00:00