Abstract:
:Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies.
journal_name
Annu Rev Biophysjournal_title
Annual review of biophysicsauthors
Romei MG,Boxer SGdoi
10.1146/annurev-biophys-051013-022846subject
Has Abstractpub_date
2019-05-06 00:00:00pages
19-44eissn
1936-122Xissn
1936-1238journal_volume
48pub_type
杂志文章,评审abstract::The transcription cycle can be roughly divided into three stages: initiation, elongation, and termination. Understanding the molecular events that regulate all these stages requires a dynamic view of the underlying processes. The development of techniques to visualize and quantify transcription in single living cells ...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-062215-010838
更新日期:2016-07-05 00:00:00
abstract::The biochemical processes leading to the synthesis of new proteins are random, as they typically involve a small number of diffusing molecules. They lead to fluctuations in the number of proteins in a single cell as a function of time and to cell-to-cell variability of protein abundances. These in turn can lead to phe...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-083012-130401
更新日期:2013-01-01 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-050511-102310
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-101209-104901
更新日期:2010-01-01 00:00:00
abstract::The living cell is an incredibly complex entity, and the goal of predictively and quantitatively understanding its function is one of the next great challenges in biology. Much of what we know about the cell concerns its constituent parts, but to a great extent we have yet to decode how these parts are organized to yi...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev.biophys.050708.133652
更新日期:2010-01-01 00:00:00
abstract::Sound-induced mechanical stimuli are detected by elaborate mechanosensory transduction (MT) machinery in highly specialized hair cells of the inner ear. Genetic studies of inherited deafness in the past decades have uncovered several molecular constituents of the MT complex, and intense debate has surrounded the molec...
journal_title:Annual review of biophysics
pub_type: 杂志文章
doi:10.1146/annurev-biophys-062420-081842
更新日期:2020-12-07 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
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更新日期:2011-01-01 00:00:00
abstract::Self-cleaving hammerhead, hairpin, hepatitis delta virus, and glmS ribozymes comprise a family of small catalytic RNA motifs that catalyze the same reversible phosphodiester cleavage reaction, but each motif adopts a unique structure and displays a unique array of biochemical properties. Recent structural, biochemical...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev.biophys.050708.133710
更新日期:2009-01-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2018-05-20 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-042910-155306
更新日期:2012-01-01 00:00:00
abstract::NF-κB (nuclear factor kappa B) family transcription factors are master regulators of immune and inflammatory processes in response to both injury and infection. In the latent state, NF-κBs are sequestered in the cytosol by their inhibitor IκB (inhibitor of NF-κB) proteins. Upon stimulations of innate immune receptors ...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-083012-130338
更新日期:2013-01-01 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章
doi:10.1146/annurev-biophys-082520-080201
更新日期:2021-01-04 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-070816-033647
更新日期:2017-05-22 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-070816-034125
更新日期:2017-05-22 00:00:00
abstract::My research began with theory and methods for ultracentrifugal studies of proteins, first at the University of Wisconsin, Madison, with Bob Alberty and Jack Williams, then at Oxford University with A.G. ("Sandy") Ogston, and finally back at Wisconsin with Williams and Lou Gosting. In 1959 I joined Arthur Kornberg's Bi...
journal_title:Annual review of biophysics
pub_type: 传,历史文章,杂志文章,评审
doi:10.1146/annurev.biophys.37.032807.125948
更新日期:2008-01-01 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-052118-115647
更新日期:2019-05-06 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-052118-115500
更新日期:2019-05-06 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-070317-032905
更新日期:2018-05-20 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-052118-115342
更新日期:2019-05-06 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-121219-081629
更新日期:2020-05-06 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev.biophys.050708.133637
更新日期:2009-01-01 00:00:00
abstract::Equilibrium sampling of biomolecules remains an unmet challenge after more than 30 years of atomistic simulation. Efforts to enhance sampling capability, which are reviewed here, range from the development of new algorithms to parallelization to novel uses of hardware. Special focus is placed on classifying algorithms...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-042910-155255
更新日期:2011-01-01 00:00:00
abstract::Proteins and other macromolecules can act as molecular machines that convert energy from one form to another through cycles of conformational transitions. In a macroscopically fluctuating environment or at the single-molecule level, the probability for a molecule to be in any state j fluctuates, and the probability cu...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-042910-155355
更新日期:2011-01-01 00:00:00
abstract::It has been known for many decades that cell surface, soluble-secreted, and extracellular matrix proteins are generally rich in disulfide bonds, but only more recently has the functional diversity of disulfide bonding in extracellular proteins been appreciated. In addition to the classic mechanisms by which disulfide ...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-050511-102321
更新日期:2012-01-01 00:00:00
abstract::Cells actively sense the mechanical properties of the extracellular matrix, such as its rigidity, morphology, and deformation. The cell-matrix interaction influences a range of cellular processes, including cell adhesion, migration, and differentiation, among others. This article aims to review some of the recent prog...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-051013-023102
更新日期:2015-01-01 00:00:00
abstract::Since the year 2000 a number of large RNA three-dimensional structures have been determined by X-ray crystallography. Structures composed of more than 100 nucleotide residues include the signal recognition particle RNA, group I intron, the GlmS ribozyme, RNAseP RNA, and ribosomal RNAs from Haloarcula morismortui, Esch...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev.biophys.36.040306.132755
更新日期:2008-01-01 00:00:00
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journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-070317-033309
更新日期:2018-05-20 00:00:00
abstract::In the past decade, a concerted effort to successfully capture specific tertiary packing interactions produced specific three-dimensional structures for many de novo designed proteins that are validated by nuclear magnetic resonance and/or X-ray crystallographic techniques. However, the success rate of computational d...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-083012-130315
更新日期:2013-01-01 00:00:00
abstract::RNA is the fundamental information transfer system in the cell. The ability to follow single messenger RNAs (mRNAs) from transcription to degradation with fluorescent probes gives quantitative information about how the information is transferred from DNA to proteins. This review focuses on the latest technological dev...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev-biophys-070317-033037
更新日期:2018-05-20 00:00:00
abstract::Imaging mass spectrometry combines the power of mass spectrometry to identify complex molecules based on mass with sample imaging. Recent advances in secondary ion mass spectrometry have improved sensitivity and spatial resolution, so that these methods have the potential to bridge between high-resolution structures o...
journal_title:Annual review of biophysics
pub_type: 杂志文章,评审
doi:10.1146/annurev.biophys.050708.133634
更新日期:2009-01-01 00:00:00