Carbon Fate and Flux in Prochlorococcus under Nitrogen Limitation.

Abstract:

:Primary production by Prochlorococcus, the smallest known free-living photosynthetic organism in terms of both physical and genomic size, is thought to have a significant role in global carbon cycles. Despite its small size and low growth rate, Prochlorococcus numerically dominates the phytoplankton community in the nutrient-poor oligotrophic ocean, the largest biome of the Earth's surface. How nutrient limitation, and nitrogen limitation in particular, affects the fate and flux of carbon fixed by Prochlorococcus is currently unknown. To address this gap in knowledge, we compared the bulk rates of photosynthesis and organic carbon release, the concentrations of intracellular metabolites, and the rates of assimilated carbon into the metabolite pools between replete and N-limited chemostat cultures. Total photosynthesis of our N-limited cultures was less than half of those observed in replete cultures, and nitrogen limitation also appears to cause a larger proportion of total fixed carbon to be released to the environment. Our data suggest this occurs in concert with the maintenance of large slow-moving pools of metabolites, including nitrogen-rich molecules such as glutamate. Additionally, we report field data suggesting metabolisms of Prochlorococcus are comparable to results we observe in our laboratory studies. Accounting for these observations, potential metabolic mechanisms utilized by Prochlorococcus are discussed as we build upon our understanding of nutrient-limited photosynthesis and carbon metabolism. IMPORTANCE Photosynthetic microbes are the predominant sources of organic carbon in the sunlit regions of the ocean. During photosynthesis, nitrogen and carbon metabolism are coordinated to synthesize nitrogen-containing organics such as amino acids and nucleic acids. In large regions of the ocean, nitrogen is thought to limit the growth of phytoplankton. The impact of nitrogen limitation on the synthesis of organic carbon is not well understood, especially for the most abundant photosynthetic organism in the nitrogen-limited regions of the ocean, Prochlorococcus. This study compares the carbon metabolism of nitrogen-replete and nitrogen-limited Prochlorococcus spp. to determine how nitrogen availability influences inorganic carbon assimilation into an organic form. Metabolomics and physiological data revealed that cells under nitrogen limitation have reduced metabolic flux and total carbon fixation rates while maintaining elevated metabolite pool levels and releasing a larger proportion of total fixed carbon to the environment.

journal_name

mSystems

journal_title

mSystems

authors

Szul MJ,Dearth SP,Campagna SR,Zinser ER

doi

10.1128/mSystems.00254-18

subject

Has Abstract

pub_date

2019-02-26 00:00:00

issue

1

issn

2379-5077

pii

mSystems00254-18

journal_volume

4

pub_type

杂志文章

相关文献

mSystems文献大全
  • Selected Immunological Mediators and Cervical Microbial Signatures in Women with Chlamydia trachomatis Infection.

    abstract::In the female genital ecosystem, the complex interplay between the host immune system and the resident microflora protects against urogenital pathogens, like Chlamydia trachomatisC. trachomatis is responsible for urethritis and cervicitis; however, most chlamydial infections are asymptomatic and, thus, not treated, po...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00094-19

    authors: Filardo S,Di Pietro M,Tranquilli G,Latino MA,Recine N,Porpora MG,Sessa R

    更新日期:2019-06-04 00:00:00

  • Galacto-Oligosaccharides Modulate the Juvenile Gut Microbiome and Innate Immunity To Improve Broiler Chicken Performance.

    abstract::Improvements in growth performance and health are key goals in broiler chicken production. Inclusion of prebiotic galacto-oligosaccharides (GOS) in broiler feed enhanced the growth rate and feed conversion of chickens relative to those obtained with a calorie-matched control diet. Comparison of the cecal microbiota id...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00827-19

    authors: Richards PJ,Flaujac Lafontaine GM,Connerton PL,Liang L,Asiani K,Fish NM,Connerton IF

    更新日期:2020-01-14 00:00:00

  • Comparative Genomics Reveals Ecological and Evolutionary Insights into Sponge-Associated Thaumarchaeota.

    abstract::Thaumarchaeota are frequently reported to associate with marine sponges (phylum Porifera); however, little is known about the features that distinguish them from their free-living thaumarchaeal counterparts. In this study, thaumarchaeal metagenome-assembled genomes (MAGs) were reconstructed from metagenomic data sets ...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00288-19

    authors: Zhang S,Song W,Wemheuer B,Reveillaud J,Webster N,Thomas T

    更新日期:2019-08-13 00:00:00

  • Integrating CRISPR-Enabled Trackable Genome Engineering and Transcriptomic Analysis of Global Regulators for Antibiotic Resistance Selection and Identification in Escherichia coli.

    abstract::It is important to expedite our understanding of antibiotic resistance to address the increasing numbers of fatalities and environmental pollution due to the emergence of antibiotic resistance and multidrug-resistant strains. Here, we combined the CRISPR-enabled trackable genome engineering (CREATE) technology and tra...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00232-20

    authors: Chen C,Choudhury A,Zhang S,Garst AD,Song X,Liu X,Chen T,Gill RT,Wang Z

    更新日期:2020-04-21 00:00:00

  • Genome Reduction and Microbe-Host Interactions Drive Adaptation of a Sulfur-Oxidizing Bacterium Associated with a Cold Seep Sponge.

    abstract::As the most ancient metazoan, sponges have established close relationships with particular microbial symbionts. However, the characteristics and physiology of thioautotrophic symbionts in deep-sea sponges are largely unknown. Using a tailored "differential coverage binning" method on 22-Gb metagenomic sequences, we re...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00184-16

    authors: Tian RM,Zhang W,Cai L,Wong YH,Ding W,Qian PY

    更新日期:2017-03-21 00:00:00

  • Dynamic Changes of Gut Microbial Communities of Bumble Bee Queens through Important Life Stages.

    abstract::Bumble bees are important pollinators in natural and agricultural ecosystems. Their social colonies are founded by individual queens, which, as the predominant reproductive females of colonies, contribute to colony function through worker production and fitness through male and new queen production. Therefore, queen h...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00631-19

    authors: Wang L,Wu J,Li K,Sadd BM,Guo Y,Zhuang D,Zhang Z,Chen Y,Evans JD,Guo J,Zhang Z,Li J

    更新日期:2019-12-10 00:00:00

  • Identification of Pathogenicity-Associated Loci in Klebsiella pneumoniae from Hospitalized Patients.

    abstract::Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients, but the factors that de...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00015-18

    authors: Martin RM,Cao J,Wu W,Zhao L,Manthei DM,Pirani A,Snitkin E,Malani PN,Rao K,Bachman MA

    更新日期:2018-06-26 00:00:00

  • Ecology and Host Identity Outweigh Evolutionary History in Shaping the Bat Microbiome.

    abstract::Recent studies of mammalian microbiomes have identified strong phylogenetic effects on bacterial community composition. Bats (Mammalia: Chiroptera) are among the most speciose mammals on the planet and the only mammal capable of true flight. We examined 1,236 16S rRNA amplicon libraries of the gut, oral, and skin micr...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00511-19

    authors: Lutz HL,Jackson EW,Webala PW,Babyesiza WS,Kerbis Peterhans JC,Demos TC,Patterson BD,Gilbert JA

    更新日期:2019-11-12 00:00:00

  • Exploring Linkages between Taxonomic and Functional Profiles of the Human Microbiome.

    abstract::Microbiome studies typically focus on characterizing the taxonomic and functional profiles of the microbes within a community. Functional profiling is generally thought to be superior to taxonomic profiling for investigating human-microbe interactions, but there are several limitations and challenges to existing appro...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00163-17

    authors: Langille MGI

    更新日期:2018-03-27 00:00:00

  • Metabolic Modeling of Streptococcus mutans Reveals Complex Nutrient Requirements of an Oral Pathogen.

    abstract::Streptococcus mutans is a Gram-positive bacterium that thrives under acidic conditions and is a primary cause of tooth decay (dental caries). To better understand the metabolism of S. mutans on a systematic level, we manually constructed a genome-scale metabolic model of the S. mutans type strain UA159. The model, cal...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00529-19

    authors: Jijakli K,Jensen PA

    更新日期:2019-10-29 00:00:00

  • Altered Bacterial-Fungal Interkingdom Networks in the Guts of Ankylosing Spondylitis Patients.

    abstract::Intestinal bacterial dysbiosis has been increasingly linked to ankylosing spondylitis (AS), which is a prototypic and best studied subtype of spondyloarthritis (SpA). Fungi and bacteria coexist in the human gut and interact with each other. Although they have been shown to contribute actively to health or disease, no ...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00176-18

    authors: Li M,Dai B,Tang Y,Lei L,Li N,Liu C,Ge T,Zhang L,Xu Y,Hu Y,Li P,Zhang Y,Yuan J,Li X

    更新日期:2019-03-26 00:00:00

  • SARS-CoV-2 Titers in Wastewater Are Higher than Expected from Clinically Confirmed Cases.

    abstract::Wastewater surveillance represents a complementary approach to clinical surveillance to measure the presence and prevalence of emerging infectious diseases like the novel coronavirus SARS-CoV-2. This innovative data source can improve the precision of epidemiological modeling to understand the penetrance of SARS-CoV-2...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00614-20

    authors: Wu F,Zhang J,Xiao A,Gu X,Lee WL,Armas F,Kauffman K,Hanage W,Matus M,Ghaeli N,Endo N,Duvallet C,Poyet M,Moniz K,Washburne AD,Erickson TB,Chai PR,Thompson J,Alm EJ

    更新日期:2020-07-21 00:00:00

  • Chemical Profiling Provides Insights into the Metabolic Machinery of Hydrocarbon-Degrading Deep-Sea Microbes.

    abstract::Marine microbes are known to degrade hydrocarbons; however, microbes inhabiting deep-sea sediments remain largely unexplored. Previous studies into the classical pathways of marine microbial metabolism reveal diverse chemistries; however, metabolic profiling of marine microbes cultured with hydrocarbons is limited. In...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00824-20

    authors: Moreno-Ulloa A,Sicairos Diaz V,Tejeda-Mora JA,Macias Contreras MI,Castillo FD,Guerrero A,Gonzalez Sanchez R,Mendoza-Porras O,Vazquez Duhalt R,Licea-Navarro A

    更新日期:2020-11-10 00:00:00

  • Pseudomonas aeruginosa Alters Its Transcriptome Related to Carbon Metabolism and Virulence as a Possible Survival Strategy in Blood from Trauma Patients.

    abstract::Trauma patients (TPs) are highly susceptible to infections, which often lead to sepsis. Among the numerous causative agents, Pseudomonas aeruginosa is especially important, as P. aeruginosa sepsis is often fatal. Understanding the mechanism of its pathogenesis in bloodstream infections is imperative; however, this mec...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00312-18

    authors: Elmassry MM,Mudaliar NS,Kottapalli KR,Dissanaike S,Griswold JA,San Francisco MJ,Colmer-Hamood JA,Hamood AN

    更新日期:2019-05-07 00:00:00

  • Data-Driven Models Reveal Mutant Cell Behaviors Important for Myxobacterial Aggregation.

    abstract::Single mutations frequently alter several aspects of cell behavior but rarely reveal whether a particular statistically significant change is biologically significant. To determine which behavioral changes are most important for multicellular self-organization, we devised a new methodology using Myxococcus xanthus as ...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00518-20

    authors: Zhang Z,Cotter CR,Lyu Z,Shimkets LJ,Igoshin OA

    更新日期:2020-07-14 00:00:00

  • Microbial Metabolic Redundancy Is a Key Mechanism in a Sulfur-Rich Glacial Ecosystem.

    abstract::Biological sulfur cycling in polar, low-temperature ecosystems is an understudied phenomenon in part due to difficulty of access and the dynamic nature of glacial environments. One such environment where sulfur cycling is known to play an important role in microbial metabolisms is located at Borup Fiord Pass (BFP) in ...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00504-20

    authors: Trivedi CB,Stamps BW,Lau GE,Grasby SE,Templeton AS,Spear JR

    更新日期:2020-08-04 00:00:00

  • Sex Bias in Gut Microbiome Transmission in Newly Paired Marmosets (Callithrix jacchus).

    abstract::Social behavior can alter the microbiome composition via transmission among social partners, but there have been few controlled experimental studies of gut microbiome transmission among social partners in primates. We collected longitudinal fecal samples from eight unrelated male-female pairs of marmoset monkeys prior...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00910-19

    authors: Zhu L,Clayton JB,Suhr Van Haute MJ,Yang Q,Hassenstab HR,Mustoe AC,Knights D,Benson AK,French JA

    更新日期:2020-03-24 00:00:00

  • Mycobacterium Phage Butters-Encoded Proteins Contribute to Host Defense against Viral Attack.

    abstract::A diverse set of prophage-mediated mechanisms protecting bacterial hosts from infection has been recently uncovered within cluster N mycobacteriophages isolated on the host, Mycobacterium smegmatis mc2155. In that context, we unveil a novel defense mechanism in cluster N prophage Butters. By using bioinformatics analy...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00534-20

    authors: Mageeney CM,Mohammed HT,Dies M,Anbari S,Cudkevich N,Chen Y,Buceta J,Ware VC

    更新日期:2020-10-06 00:00:00

  • The Dimension of Time in Host-Microbiome Interactions.

    abstract::The intestinal microbiota contains trillions of commensal microorganisms that shape multiple aspects of host physiology and disease. In contrast to the host's genome, the microbiome is amenable to change over the course of an organism's lifetime, providing an opportunity to therapeutically modulate the microbiome's im...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00216-18

    authors: Uhr GT,Dohnalová L,Thaiss CA

    更新日期:2019-02-19 00:00:00

  • Getting the Hologenome Concept Right: an Eco-Evolutionary Framework for Hosts and Their Microbiomes.

    abstract::Given the complexity of host-microbiota symbioses, scientists and philosophers are asking questions at new biological levels of hierarchical organization-what is a holobiont and hologenome? When should this vocabulary be applied? Are these concepts a null hypothesis for host-microbe systems or limited to a certain spe...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00028-16

    authors: Theis KR,Dheilly NM,Klassen JL,Brucker RM,Baines JF,Bosch TC,Cryan JF,Gilbert SF,Goodnight CJ,Lloyd EA,Sapp J,Vandenkoornhuyse P,Zilber-Rosenberg I,Rosenberg E,Bordenstein SR

    更新日期:2016-03-29 00:00:00

  • Probiotic Bifidobacterium lactis V9 Regulates the Secretion of Sex Hormones in Polycystic Ovary Syndrome Patients through the Gut-Brain Axis.

    abstract::Although a few studies have investigated the intestinal microbiota of women with polycystic ovary syndrome (PCOS), the functional and metabolic mechanisms of the microbes associated with PCOS, as well as potential microbial biomarkers, have not yet been identified. To address this gap, we designed a two-phase experime...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00017-19

    authors: Zhang J,Sun Z,Jiang S,Bai X,Ma C,Peng Q,Chen K,Chang H,Fang T,Zhang H

    更新日期:2019-04-16 00:00:00

  • Comparative Genomics and Metabolomics in the Genus Nocardia.

    abstract::Using automated genome analysis tools, it is often unclear to what degree genetic variability in homologous biosynthetic pathways relates to structural variation. This hampers strain prioritization and compound identification and can lead to overinterpretation of chemical diversity. Here, we assessed the metabolic pot...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00125-20

    authors: Männle D,McKinnie SMK,Mantri SS,Steinke K,Lu Z,Moore BS,Ziemert N,Kaysser L

    更新日期:2020-06-02 00:00:00

  • A Complex Interplay between Nitric Oxide, Quorum Sensing, and the Unique Secondary Metabolite Tundrenone Constitutes the Hypoxia Response in Methylobacter.

    abstract::Methylobacter species, members of the Methylococcales, have recently emerged as some of the globally widespread, cosmopolitan species that play a key role in the environmental consumption of methane across gradients of dioxygen tensions. In this work, we approached the question of how Methylobacter copes with hypoxia,...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00770-19

    authors: Yu Z,Pesesky M,Zhang L,Huang J,Winkler M,Chistoserdova L

    更新日期:2020-01-21 00:00:00

  • Differential Gene Expression of Three Mastitis-Causing Escherichia coli Strains Grown under Planktonic, Swimming, and Swarming Culture Conditions.

    abstract::Bacterial motility is thought to play an important role in virulence. We have previously shown that proficient bacterial swimming and swarming in vitro is correlated with the persistent intramammary infection phenotype observed in cattle. However, little is known about the gene regulation differences important for dif...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00064-16

    authors: Lippolis JD,Brunelle BW,Reinhardt TA,Sacco RE,Thacker TC,Looft TP,Casey TA

    更新日期:2016-08-09 00:00:00

  • Computational Genomics of Specialized Metabolism: from Natural Product Discovery to Microbiome Ecology.

    abstract::Microbial and plant specialized metabolites, also known as natural products, are key mediators of microbe-microbe and host-microbe interactions and constitute a rich resource for drug development. In the past decade, genome mining has emerged as a prominent strategy for natural product discovery. Initially, such minin...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00182-17

    authors: Medema MH

    更新日期:2018-03-06 00:00:00

  • Microbiota-Derived Short-Chain Fatty Acids Promote LAMTOR2-Mediated Immune Responses in Macrophages.

    abstract::ABSRTACTKlebsiella pneumoniae is a common cause of human-pneumonia-derived sepsis with high morbidity and mortality. The microbiota promotes and maintains host immune homeostasis. The mechanisms by which the gut microbiota affects the host defenses in the respiratory system systematically, however, remain poorly under...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00587-20

    authors: Wu T,Li H,Su C,Xu F,Yang G,Sun K,Xu M,Lv N,Meng B,Liu Y,Hu L,Liu Y,Gao Y,Wang H,Lan Y,Xu D,Li J

    更新日期:2020-11-03 00:00:00

  • Predicting Phenotypic Polymyxin Resistance in Klebsiella pneumoniae through Machine Learning Analysis of Genomic Data.

    abstract::Polymyxins are used as treatments of last resort for Gram-negative bacterial infections. Their increased use has led to concerns about emerging polymyxin resistance (PR). Phenotypic polymyxin susceptibility testing is resource intensive and difficult to perform accurately. The complex polygenic nature of PR and our in...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00656-19

    authors: Macesic N,Bear Don't Walk OJ IV,,Pe'er I,Tatonetti NP,Peleg AY,Uhlemann AC

    更新日期:2020-05-26 00:00:00

  • A Decrease in Transcription Capacity Limits Growth Rate upon Translation Inhibition.

    abstract::In bacterial cells, inhibition of ribosomes by sublethal concentrations of antibiotics leads to a decrease in the growth rate despite an increase in ribosome content. The limitation of ribosomal activity results in an increase in the level of expression from ribosomal promoters; this can deplete the pool of RNA polyme...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00575-20

    authors: Zhang Q,Brambilla E,Li R,Shi H,Cosentino Lagomarsino M,Sclavi B

    更新日期:2020-09-08 00:00:00

  • Diagnosing and Predicting Mixed-Culture Fermentations with Unicellular and Guild-Based Metabolic Models.

    abstract::Multispecies microbial communities determine the fate of materials in the environment and can be harnessed to produce beneficial products from renewable resources. In a recent example, fermentations by microbial communities have produced medium-chain fatty acids (MCFAs). Tools to predict, assess, and improve the perfo...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00755-20

    authors: Scarborough MJ,Hamilton JJ,Erb EA,Donohue TJ,Noguera DR

    更新日期:2020-09-29 00:00:00

  • Metatranscriptomics Supports the Mechanism for Biocathode Electroautotrophy by "Candidatus Tenderia electrophaga".

    abstract::Biocathodes provide a stable electron source to drive reduction reactions in electrotrophic microbial electrochemical systems. Electroautotrophic biocathode communities may be more robust than monocultures in environmentally relevant settings, but some members are not easily cultivated outside the electrode environmen...

    journal_title:mSystems

    pub_type: 杂志文章

    doi:10.1128/mSystems.00002-17

    authors: Eddie BJ,Wang Z,Hervey WJ 4th,Leary DH,Malanoski AP,Tender LM,Lin B,Strycharz-Glaven SM

    更新日期:2017-03-28 00:00:00