Abstract:
:To explore the antitumor effect of hypoxia-inducible factor-1α short hairpin RNA (HIF-1α shRNA) delivered by ultrasound targeted microbubble destruction (UTMD) and transcatheter arterial embolization (TAE) on rats with hepatic cancer. After the models of transplantation hepatoma were established, Wistar rats were randomly divided into 4 groups: Control group, UTMD group, TAE group, and UTMD+TAE group. Contrast-enhanced ultrasound (CEUS) was used to monitor tumor size on day 14 after four different treatments. Western blotting and immunohistochemistry were applied to measure the protein level of HIF-1α and VEGF in the hepatic cancer tissue. In comparison with UTMD+TAE group (21.25±10.68 days), the mean survival time was noticeably shorter in the Control group and TAE group (13.02±4.30 days and 15.03±7.32 days) (p<0.05, respectively). There was no statistical difference between UTMD+TAE group and UTMD group of the mean survival time (p>0.05). In addition, our results proved that the tumor sizes in UTMD+TAE group were obviously smaller than those in other groups (p<0.05, respectively). By CEUS, we clearly found that the tumor size was the smallest on day 14 in the UTMD+TAE group. The western blotting and immunohistochemistry results proved that the protein levels of HIF-1α and VEGF in UTMD+TAE group were obviously lower than those in TAE group and Control group on days 7 and 14 (p<0.05, respectively). However, there was no statistical difference between UTMD+TAE group and UTMD group (p>0.05). In this study we tried to explore the antitumor effect through a combination of UTMD-mediated HIF-1α shRNA transfection and TAE on rats with hepatic cancer. Our results showed that UTMD-mediated HIF-1α shRNA transfection and TAE can obviously silence HIF-1α and VEGF expression, thereby successfully inhibiting the growth of the tumor.
journal_name
Biomed Res Intjournal_title
BioMed research internationalauthors
Liao Y,Luo H,He Z,Kuang Y,Chen P,Zhang X,Chen J,Wen Q,Xie Y,Ding Sdoi
10.1155/2019/1937460subject
Has Abstractpub_date
2019-02-19 00:00:00pages
1937460eissn
2314-6133issn
2314-6141journal_volume
2019pub_type
杂志文章abstract::Numerous studies have reported that mesenchymal stem cells (MSCs) can ameliorate neurological deficits in ischemic stroke models. Among the various hypotheses that have been suggested to explain the therapeutic mechanism underlying these observations, neurogenesis is thought to be critical. To enhance the therapeutic ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/129145
更新日期:2014-01-01 00:00:00
abstract::Virulence-associated traits have frequently been studied in enterococci and are considered to contribute towards the pathogenicity of infections. In the present study, Enterococcus isolates were collected during diagnostic investigations from meat turkeys in Germany. Twenty-eight isolates of three different Enterococc...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/6147695
更新日期:2019-10-30 00:00:00
abstract::Picobirnavirus (PBV) which has been included in the list of viruses causing enteric infection in animals is highly versatile because of its broad host range and genetic diversity. PBVs are among the most recent and emerging small, nonenveloped viruses with a bisegmented double-stranded RNA genome, classified under a n...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2014/780752
更新日期:2014-01-01 00:00:00
abstract::We applied a metabolomic approach to monitor the modifications occurring in goat vitreous humor (VH) metabolite composition at different times (0, 6, 12, 18, and 24 hours) after death. The (1)H-NMR analysis of the VH samples was performed for the simultaneous determination of several metabolites (i.e., the metabolite ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/627201
更新日期:2015-01-01 00:00:00
abstract:Background:Osteoarthritis (OA) is a common disease characterized by articular cartilage degeneration and secondary hyperosteogenesis. Genetic factors are associated with the occurrence of OA. While several studies have shown that the matrix metalloproteinase-1- (MMP-1-) 1607 1G/2G (rs1799750) polymorphism may be relate...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2020/5190587
更新日期:2020-05-20 00:00:00
abstract::Background. Neuroinflammation which presents as a possible mechanism of delirium is associated with MCP-1, an important proinflammatory factor which is expressed on astrocytes. It is known that dexmedetomidine (DEX) possesses potent anti-inflammatory properties. This study aimed to investigate the potential effects of...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/6352159
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Recently antiangiogenic therapy with bevacizumab has shown a high but transient efficacy in glioblastoma (GBM). Indeed, GBM is one of the most angiogenic human tumors and endothelial proliferation is a hallmark of the disease. We therefore hypothesized that tumor cells may participate in endothelial prolifer...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/827327
更新日期:2014-01-01 00:00:00
abstract:Background:Since the dawn of human history, foodborne diseases have been a problem for all societies, and it is an increasing public health issue worldwide. The objective of this study is to examine knowledge, practice, and attitude in food safety among college students in the city Gondar, northwest Ethiopia. Methods:...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2021/6686392
更新日期:2021-01-12 00:00:00
abstract::Effects of ketamine and lidocaine on electroencephalographic (EEG) changes were evaluated in minimally anaesthetized dogs, subjected to electric stimulus. Six dogs were subjected to six treatments in a crossover design with a washout period of one week. Dogs were subjected to intravenous boluses of lidocaine 2 mg/kg, ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/305367
更新日期:2015-01-01 00:00:00
abstract::Adipose-derived stem cells (ADSCs) are an abundant cell source and provide an easy way to harvest mesenchymal stem cells, which are the focus of considerable attention in regenerative medicine. Electric fields (EF) play roles in many biological events and have been reported to promote cell proliferation, migration, an...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/7134719
更新日期:2020-11-06 00:00:00
abstract::Different opinions exist on the relationship between the C-reactive protein-to-albumin ratio (CAR) and the prognosis of colon cancer. This study is aimed at evaluating the relationship between CAR and prognosis of stage II-III colon cancer and establishing a clinical prognosis model. Patients were randomised to a trai...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/8812974
更新日期:2020-11-22 00:00:00
abstract::Iodinated contrast (IC) is clinically used in diagnostic and interventional procedures, but its use can result in contrast-induced acute kidney injury (CI-AKI). Chronic kidney disease (CKD) and chronic hyperglycemia (CH) are important predisposing factors to CI-AKI. The aim of this study was to investigate the impact ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/3019410
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:To investigate the availability of correction of mandibular plane using software for vertical measurements in cone beam computed tomography (CBCT) according to the sites of the mandible. METHODS:CBCT scans of six dry mandibles were performed at 0-, 5-, 10-, 15-, and 20-degree angles relative to CBCT scannin...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/808625
更新日期:2015-01-01 00:00:00
abstract::Although considerable progress has been made in the management of patients with invasive fungal infections, Candida bloodstream infections are still widespread in hospital settings. Incidence rates vary geographically, often because of different patient populations. The aim of the present study was to describe the epi...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/256580
更新日期:2015-01-01 00:00:00
abstract::Epithelial Cl(-) secretion plays important roles in water secretion preventing bacterial/viral infection and regulation of body fluid. We previously suggested that quercetin would be a useful compound for maintaining epithelial Cl(-) secretion at a moderate level irrespective of cAMP-induced stimulation. However, we n...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2014/902735
更新日期:2014-01-01 00:00:00
abstract::Although the dynamic motions and peptidyl transferase activity seem to be embedded in the rRNAs, the ribosome contains more than 50 ribosomal proteins (r-proteins), whose functions remain largely elusive. Also, the precise forms of some of these r-proteins, as being part of the ribosome, are not structurally solved du...
journal_title:BioMed research international
pub_type: 杂志文章,评审
doi:10.1155/2015/539238
更新日期:2015-01-01 00:00:00
abstract::It has been reported that many modifications occur with the increase of oxidative stress during storage in erythrocytes. In order to delay these negative changes, we evaluated whether the addition of substances likely to protect antioxidant capacity in stored blood would be useful. Therefore, we investigated the effec...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/3057384
更新日期:2016-01-01 00:00:00
abstract::An excessive pulmonary inflammatory response could explain the poor prognosis of chronic obstructive pulmonary disease (COPD) patients submitted to invasive mechanical ventilation. The aim of this study was to evaluate the response to normal tidal volume mechanical ventilation in an elastase-induced murine model of pu...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2018/5721293
更新日期:2018-12-19 00:00:00
abstract:BACKGROUND:This study aimed to analyze differences in necrotic changes between cortical and cancellous bone in resection margins after segmental mandibulectomy for advanced mandibular osteoradionecrosis. METHODS:Anteroposterior bone specimens from eleven patients who underwent segmental mandibulectomy with simultaneou...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/3125842
更新日期:2017-01-01 00:00:00
abstract:UNLABELLED:Ehlers-Danlos syndrome hypermobility type a. k. a. joint hypermobility syndrome (JHS/EDS-HT) is a hereditary musculoskeletal disorder associating generalized joint hypermobility with chronic pain. Anecdotal reports suggest a prominent role for kinesiophobia in disease manifestations, but no study has systema...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/580460
更新日期:2013-01-01 00:00:00
abstract:Objective:The aim of this study is to explore the potential pathogenesis of juvenile dermatomyositis by bioinformatics analysis of gene chips, which would screen the hub genes, identify potential biomarkers, and reveal the development mechanism of juvenile dermatomyositis. Material and Methods:We retrieved juvenile de...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/7814287
更新日期:2019-12-07 00:00:00
abstract::Human epididymis protein 4 (HE4) is an available tumor biomarker mainly for detecting ovarian cancer. However, it is unknown whether it can be a novel indicator for diagnosis of diabetic kidney disease (DKD). The aim of this study was to investigate the possibility of serum HE4 as a novel biomarker for DKD in patients...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2019/4831459
更新日期:2019-11-11 00:00:00
abstract::POZ/BTB domains are widespread modules detected in a variety of different biological contexts. Here, we report a biophysical characterization of the POZ/BTB of KCTD6, a protein that is involved in the turnover of the muscle small ankyrin-1 isoform 5 and, in combination with KCTD11, in the ubiquitination and degradatio...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/162674
更新日期:2013-01-01 00:00:00
abstract::To protect against the environmental pollution, the present research was undertaken to enumerate the Bacterial Technologies (BTs) on the restoration of polluted urban rivers, that is, Fenghu-Song Yang River (FSR) and Xuxi River (XXR). Experimental research accounted for the physiochemical parameters (pH; temperature; ...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/241769
更新日期:2015-01-01 00:00:00
abstract::Hosts' innate defense systems are upregulated by antimicrobial peptide elicitors (APEs). Our aim was to investigate the effects of hyperthermia, ultraviolet A rays (UVA), and ultraviolet C rays (UVC) as well as glucose and ascorbic acid (AA) on the regulation of human β-defensin 1 (DEFB1), cathelicidin (CAMP), and int...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2015/714580
更新日期:2015-01-01 00:00:00
abstract::Bioadhesive nasal dosage forms are an attractive method for overcoming rapid mucociliary clearance transport in the nose and for delivering the drug directly to brain. The present study was designed to formulate chondroitin sulphate (CS) and chitosan (CH) nasal inserts employing zolmitriptan, an antimigraine drug. The...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2013/958465
更新日期:2013-01-01 00:00:00
abstract::Shigellosis is a global human health problem and the incidence is highest among children. In the present work, main Shigella virulence genes was examined by PCR and compared to symptoms of pediatric shigellosis. Thirty Shigella isolates were identified from an etiologic study at which 1,339 children ranging 0-10 years...
journal_title:BioMed research international
pub_type: 临床试验,杂志文章
doi:10.1155/2014/539697
更新日期:2014-01-01 00:00:00
abstract::Ultrasound tomography (UST) image segmentation is fundamental in breast density estimation, medicine response analysis, and anatomical change quantification. Existing methods are time consuming and require massive manual interaction. To address these issues, an automatic algorithm based on GrabCut (AUGC) is proposed i...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2017/2059036
更新日期:2017-01-01 00:00:00
abstract:Background:A deficiency in steroid 5α-reductase type 2 is an autosomal recessive disorder. Affected individuals manifested ambiguous genitalia, which is caused by decreased dihydrotestosterone (DHT) synthesis in the fetus. Methods:We analyzed 25 patients with 5α-reductase deficiency in China. Seventeen of the 25 patie...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2020/1789514
更新日期:2020-06-09 00:00:00
abstract:PURPOSE:To investigate whether P. acnes could induce disc degeneration and Modic changes when inoculated into the discs of rabbits. METHOD:A wild-type strain of P. acnes isolated from a patient associated with Modic change and disc degeneration was inoculated into the intervertebral discs of rabbits. Meanwhile, S. aur...
journal_title:BioMed research international
pub_type: 杂志文章
doi:10.1155/2016/9612437
更新日期:2016-01-01 00:00:00