Abstract:
Aims:The purpose of the present research is to investigate the effects of the VHL protein antagonist, VH298, on functional activities of fibroblasts and vascular endothelial cells and the effects on the wound healing process in a streptozotocin-induced hyperglycaemic rat model. Methods:HIF-1α and hydroxy-HIF-1α protein levels in VH298-treated rat fibroblasts (rFb) were measured by immunoblotting, rFb proliferation was detected by the CCK-8 assay, and mRNA levels of related genes were measured by quantitative RT-PCR. In vitro wound healing was simulated by the scratch test; angiogenesis was measured by the human umbilical vein endothelial cell (hUVEC) tube formation assay. VH298 or PBS was locally injected into wounds in rat models with streptozotocin- (STZ-) induced hyperglycaemia, the wound tissues were harvested, and haematoxylin-eosin (HE) and Masson trichrome staining and immunohistochemical processes were conducted. Results:HIF-1α and hydroxy-HIF-1α levels increased in VH298-treated rFb, in a time- and dose-dependent manner. Thirty micromolar VH298 could significantly increase cell proliferation, angiogenesis, and gene expression of type I collagen-α1 (Col1-α1), vascular endothelial growth factor A (VEGF-A), and insulin-like growth factor 1 (IGF-1). The VH298-treated wound had a better healing pattern, activation of HIF-1 signalling, and vascularization. Conclusions:Taken together, VH298 activated the HIF-1 signalling pathway by stabilizing both HIF-1α and hydroxy-HIF-1α. VH298 enhanced rFb functions, promoted hUVEC angiogenesis, and accelerated wound healing in the rat model mimicking diabetes mellitus.
journal_name
J Diabetes Resjournal_title
Journal of diabetes researchauthors
Qiu S,Jia Y,Sun Y,Han P,Xu J,Wen G,Chai Ydoi
10.1155/2019/1897174subject
Has Abstractpub_date
2019-02-17 00:00:00pages
1897174eissn
2314-6745issn
2314-6753journal_volume
2019pub_type
杂志文章abstract:PURPOSE:The aim was to explore the effect of the chromium picolinate (CrPic) administration on the pancreas and macroangiopathy of type II diabetes mellitus rats. METHODS:The type II diabetes mellitus (T2DM) rat model was induced by low-dose streptozotocin (STZ). The rats were randomly divided into 5 groups (ten rats ...
journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2014/717219
更新日期:2014-01-01 00:00:00
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journal_title:Journal of diabetes research
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更新日期:2018-05-29 00:00:00
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更新日期:2018-03-20 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
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更新日期:2019-08-14 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
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更新日期:2020-02-06 00:00:00
abstract:Background:Diabetic retinopathy (DR) is a severe complication of diabetes mellitus. DR is considered as a neurovascular disease. Retinal ganglion cell (RGC) loss plays an important role in the vision function disorder of diabetic patients. Histone deacetylase3 (HDAC3) is closely related to injury repair and nerve regen...
journal_title:Journal of diabetes research
pub_type: 杂志文章
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更新日期:2020-03-01 00:00:00
abstract::Diabetes mellitus (DM) is a major worldwide health problem and its prevalence has been rapidly increasing in the last century. It is caused by defects in insulin secretion or insulin action or both, leading to hyperglycemia. Of the various types of DM, type 2 occurs most frequently. Multiple genes and their interactio...
journal_title:Journal of diabetes research
pub_type: 杂志文章,评审
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更新日期:2015-01-01 00:00:00
abstract::Previous studies investigating determinants of changes in glycemic status among individuals with prediabetes mainly focused on glucose-defined prediabetes. In this study, we examined determinants of a regression to normoglycemia or a progression to diabetes among individuals with HbA1c-defined prediabetes. The study i...
journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2018/5703652
更新日期:2018-10-14 00:00:00
abstract:Objective:To investigate the effects of cod-liver oil on metabolic status and high-sensitivity C-reactive protein (hs-CRP) in patients with gestational diabetes mellitus (GDM). Methods:This study was a randomized, double-blinded, placebo-controlled trial with the allocation ratio of 1 : 1. The contents of EPA and DHA ...
journal_title:Journal of diabetes research
pub_type: 杂志文章,随机对照试验
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journal_title:Journal of diabetes research
pub_type: 杂志文章
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更新日期:2018-09-02 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章,评审
doi:10.1155/2016/4270301
更新日期:2016-01-01 00:00:00
abstract::Elevated systolic pulmonary artery pressure (s-PAP, ≥35 mmHg) serves as an independent predictor of mortality in hemodialysis (HD) and diabetic (DM) patients. A polymorphism in the antioxidant Haptoglobin (Hp) gene has been shown to regulate the bioavailability of nitric oxide (NO), a major mediator of pulmonary vascu...
journal_title:Journal of diabetes research
pub_type: 杂志文章,多中心研究
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更新日期:2015-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2016/1632061
更新日期:2016-01-01 00:00:00
abstract:AIMS:This study assesses the impact of using an AP-system at home on fear of hypoglycaemia. In addition, satisfaction and acceptance of the new technology are evaluated. METHODS:In a multicentre, multinational study of 75 patients using the MD-Logic AP during four consecutive nights in home setting 59 of them (aged 10...
journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2015/590308
更新日期:2015-01-01 00:00:00
abstract:AIM:To evaluate an agreement in identifying dysglycemia between fasting plasma glucose (FPG) and the 2 hr postprandial glucose tolerance test (OGTT) in a population with high risk of diabetes. METHODS:A total of 6,884 individuals aged 35-65 years recruited for a community-based diabetes prevention program were tested ...
journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2015/396505
更新日期:2015-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2013/432659
更新日期:2013-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2016/6156217
更新日期:2016-01-01 00:00:00
abstract::Objectives. To compare the prevalence of diabetic peripheral neuropathy (DPN) and that of cardiac autonomic neuropathy (CAN) between South Asians and White Caucasians with type 2 diabetes and to explore reasons for observed differences. Methods. A cross-sectional study of casually selected South Asian and White Caucas...
journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2017/1273789
更新日期:2017-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
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abstract::Aldehyde dehydrogenase (ALDH) gene superfamily consists of 19 isozymes. They are present in various organs and involved in metabolizing aldehydes that are biologically generated. For instance, ALDH2, a cardiac mitochondrial ALDH isozyme, is known to detoxify 4-hydroxy-2-nonenal, a reactive aldehyde produced upon lipid...
journal_title:Journal of diabetes research
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2015/485759
更新日期:2015-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2016/3917806
更新日期:2016-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2016/3142175
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Intestinal dysfunction in the ob/ob mouse model of diabetes mimics that seen clinically. METHODS:We determined the effects of a 4-week genistein diet (600 mg genistein/kg food) on intestinal function (contractility, morphology, AChR, and motility) in female ob/ob and lean mice. RESULTS:Contractility of the...
journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2017/3568146
更新日期:2017-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2016/8315454
更新日期:2016-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2017/2879249
更新日期:2017-01-01 00:00:00
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pub_type: 杂志文章,随机对照试验
doi:10.1155/2016/5027356
更新日期:2016-01-01 00:00:00
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更新日期:2015-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章,meta分析,评审
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更新日期:2016-01-01 00:00:00
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journal_title:Journal of diabetes research
pub_type: 杂志文章
doi:10.1155/2019/5487962
更新日期:2019-12-17 00:00:00