Trans Complementation of Replication-defective Omsk Hemorrhagic Fever Virus for Antiviral Study.

Abstract:

:Omsk hemorrhagic fever virus (OHFV) is a tick-borne flavivirus classified as a biosafety level-4 (BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion (OHFV-ΔNS1) and reporter virus replacing NS1 with the Gaussia luciferase (Gluc) (OHFV-ΔNS1-Gluc). Both the defective OHFV-ΔNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1 (BHK21NS1) but not in naïve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z' value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories.

journal_name

Virol Sin

journal_title

Virologica Sinica

authors

Zhang Q,Li N,Deng C,Zhang Z,Li X,Yoshii K,Ye H,Zhang B

doi

10.1007/s12250-019-00109-0

subject

Has Abstract

pub_date

2019-08-01 00:00:00

pages

412-422

issue

4

eissn

1674-0769

issn

1995-820X

pii

10.1007/s12250-019-00109-0

journal_volume

34

pub_type

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