Abstract:
:Urinary tract infection (UTI) is a prominent global health care burden. Although UTI is readily treated with antibiotics in healthy adults, complicated cases in immune-compromised individuals and the emerging antibiotic resistance of several uropathogens have accelerated the need for new treatment strategies. Here, we surveyed the composition of urinary exosomes in a mouse model of uropathgenic Escherichia coli (UPEC) UTI to identify specific urinary tract defense constituents for therapeutic development. We found an enrichment of the iron-binding glycoprotein lactoferrin in the urinary exosomes of infected mice. In subsequent in vitro studies, we identified human bladder epithelial cells as a source of lactoferrin during UPEC infection. We further established that exogenous treatment with human lactoferrin (hLf) reduces UPEC epithelial adherence and enhances neutrophil antimicrobial functions including bacterial killing and extracellular trap production. Notably, a single intravesicular dose of hLf drastically reduced bladder bacterial burden and neutrophil infiltration in our murine UTI model. We propose that lactoferrin is an important modulator of innate immune responses in the urinary tract and has potential application in novel therapeutic design for UTI.
journal_name
J Innate Immunjournal_title
Journal of innate immunityauthors
Patras KA,Ha AD,Rooholfada E,Olson J,Ramachandra Rao SP,Lin AE,Nizet Vdoi
10.1159/000499342subject
Has Abstractpub_date
2019-01-01 00:00:00pages
481-495issue
6eissn
1662-811Xissn
1662-8128pii
000499342journal_volume
11pub_type
杂志文章abstract:BACKGROUND:Oncolytic vesicular stomatitis virus (VSV) can be delivered intravenously to target primary and metastatic lesions, but the interaction between human peripheral blood leukocytes (PBLs) and VSV remains poorly understood. Our study aimed to assess the overall immunological consequences of ex vivo infection of ...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000485143
更新日期:2018-01-01 00:00:00
abstract::The host innate immune response, including the production of type-I IFN, represents the primary line of defense against invading viral pathogens. Of the hundreds of IFN-stimulated genes (ISGs) discovered to date, ISG15 was one of the first identified and shown to encode a ubiquitin-like protein that functions, in part...
journal_title:Journal of innate immunity
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journal_title:Journal of innate immunity
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journal_title:Journal of innate immunity
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doi:10.1159/000398799
更新日期:2015-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000445770
更新日期:2016-01-01 00:00:00
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journal_title:Journal of innate immunity
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pub_type: 杂志文章,评审
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journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000328011
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journal_title:Journal of innate immunity
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doi:10.1159/000480386
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journal_title:Journal of innate immunity
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doi:10.1159/000339402
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000181015
更新日期:2009-01-01 00:00:00
abstract::Previously, a novel cell type, the multinucleated giant hemocyte (MGH) was identified in the ananassae subgroup of Drosophilidae. These cells share several features with mammalian multinucleated giant cells, a syncytium of macrophages formed during granulomatous inflammation. We were able to show that MGHs also differ...
journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000502646
更新日期:2020-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000315050
更新日期:2010-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000360293
更新日期:2014-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000235656
更新日期:2009-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000323920
更新日期:2011-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000339826
更新日期:2013-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000339153
更新日期:2012-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000362647
更新日期:2014-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000350242
更新日期:2013-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
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journal_title:Journal of innate immunity
pub_type: 杂志文章
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000342430
更新日期:2013-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000439052
更新日期:2016-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000317665
更新日期:2010-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章
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journal_title:Journal of innate immunity
pub_type: 杂志文章
doi:10.1159/000509111
更新日期:2020-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000342427
更新日期:2013-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000323929
更新日期:2011-01-01 00:00:00
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journal_title:Journal of innate immunity
pub_type: 杂志文章,评审
doi:10.1159/000226247
更新日期:2009-01-01 00:00:00