Abstract:
Introduction:Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. Methods:A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite. Results:Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819. Conclusion:Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.
journal_name
Afr Health Scijournal_title
African health sciencesauthors
Shahnazi M,Mehrizi MZ,Alizadeh SA,Heydarian P,Saraei M,Alipour M,Hajialilo Edoi
10.4314/ahs.v19i1.6subject
Has Abstractpub_date
2019-03-01 00:00:00pages
1346-1352issue
1eissn
1680-6905issn
1729-0503pii
jAFHS.v19.i1.pg1346journal_volume
19pub_type
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