Molecular characterization of Hymenolepis nana based on nuclear rDNA ITS2 gene marker.

Abstract:

Introduction:Hymenolepis nana is a zoonotic tapeworm with widespread distribution. The goal of the present study was to identify the parasite in the specimens collected from NorthWestern regions of Iran using PCR-sequencing method. Methods:A total of 1521 stool samples were collected from the study individuals. Initially, the identification of hymenolepis nana was confirmed by parasitological method including direct wet-mount and formalin-ethyl acetate concentration methods. Afterward, PCR-sequencing analysis of ribosomal ITS2 fragment was targeted to investigate the molecular identification of the parasite. Results:Overall, 0.65% (10/1521) of the isolates were contaminated with H. nana in formalin-ethyl acetate concentration. All ten isolates were succefully amplified by PCR and further sequenced. The determined sequences were deposited in GenBank under the accession numbers MH337810 -MH337819. Conclusion:Our results clarified the presence of H. nana among the patients in the study areas. In addition, the molecular technique could be accessible when the human eggs are the only sources available to identify and diagnose the parasite.

journal_name

Afr Health Sci

journal_title

African health sciences

authors

Shahnazi M,Mehrizi MZ,Alizadeh SA,Heydarian P,Saraei M,Alipour M,Hajialilo E

doi

10.4314/ahs.v19i1.6

subject

Has Abstract

pub_date

2019-03-01 00:00:00

pages

1346-1352

issue

1

eissn

1680-6905

issn

1729-0503

pii

jAFHS.v19.i1.pg1346

journal_volume

19

pub_type

杂志文章
  • Modern lineages of Mycobacterium tuberculosis in Addis Ababa, Ethiopia: implications for the tuberculosis control programe.

    abstract:BACKGROUND:The genotyping of Mycobacterium tuberculosis strains is important to have unique insights into the dissemination dynamics and evolutionary genetics of this pathogen and for TB control as it allows the detection of suspected outbreaks and the tracing of transmission chains. OBJECTIVE:To characterize M. tuber...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v12i3.15

    authors: Mihret A,Bekele Y,Aytenew M,Assefa Y,Abebe M,Wassie L,Loxton GA,Yamuah L,Aseffa A,Walzl G,Howe R

    更新日期:2012-09-01 00:00:00

  • Normative ultrasound values of renal parenchymal thickness among adults in Enugu, South-East Nigeria.

    abstract:BACKGROUND:Reduction in renal length was found to be an insufficient independent indicator of chronic renal disease. OBJECTIVE:To determine the ultrasound normative values of renal parenchymal thickness (RPT) among adults and correlate them with age and somatometric parameters. METHODS:This was a prospective clinic b...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v14i3.27

    authors: Eze C,Okoye J,Agwu K

    更新日期:2014-09-01 00:00:00

  • Pumpkin seed oil alleviates oxidative stress and liver damage induced by sodium nitrate in adult rats: biochemical and histological approach.

    abstract:Background:Nitrate (NO3) is the most common chemical contaminant in the world's ground water aquifer. Oxidative stress has been proposed as a possible mechanism involved in NO3 toxicity on non-target organism. Objectives:The current study aimed to elucidate the potential protective effect of Telfairia occidentalis (pu...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v20i1.48

    authors: Rouag M,Berrouague S,Djaber N,Khaldi T,Boumendjel M,Taibi F,Abdennour C,Boumendjel A,Messarah M

    更新日期:2020-03-01 00:00:00

  • Validity of common ultrasound methods of fetal weight estimation in late pregnancy among women in Kwale, Niger Delta region, Nigeria.

    abstract:BACKGROUND:Accuracy of some ultrasound equations used in our locality for fetal weight estimation is doubtful. OBJECTIVE:To assess the accuracy of common ultrasound equations used for fetal weight estimation. SUBJECTS AND METHODS:A longitudinal study was conducted on selected Nigerian obstetric population at Central ...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v15i1.27

    authors: Eze CU,Egwuanumku KI,Agwuna KK,Odidi L,Ochie K,Nwadike IU

    更新日期:2015-03-01 00:00:00

  • Impact of weight reduction on insulin resistance, adhesive molecules and adipokines dysregulation among obese type 2 diabetic patients.

    abstract:Background:Type 2 diabetes mellitus is usually related to vascular problems and is associated with impairment in endothelial function characterized by impaired endothelial-dependent vasodilation and increased platelet adhesion. There is limitation in clinical studies that have addressed the beneficial effects of weight...

    journal_title:African health sciences

    pub_type: 杂志文章,随机对照试验

    doi:10.4314/ahs.v18i4.5

    authors: Abd El-Kader SM,Al-Jiffri OH

    更新日期:2018-12-01 00:00:00

  • The hazards of conducting induction of labour in high-risk pregnancies at district hospitals in low- and middle-income countries: lessons to learn from a case report.

    abstract:Background:Induction of labour (IOL) is an obstetric procedure that should be conducted in a healthcare facility with the capacity to provide optimal care based on the patient risk status. Inadequate monitoring, untimely procedure and lack of readily available and experienced medical staff to participate in the care of...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v20i3.27

    authors: Ngene NC,Moodley J

    更新日期:2020-09-01 00:00:00

  • Blindness and scalp haematoma in a child following a snakebite.

    abstract:BACKGROUND:Snake envenomation is a major public health problem of the Savannah regions of West Africa. Ocular manifestations of snakebites are rare with few reports documenting blindness as a complication. OBJECTIVE:To highlight an unusual manifestation of snake bites and its attendant problems. METHODS:A report of s...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v15i3.46

    authors: Katibi OS,Adepoju FG,Olorunsola BO,Ernest SK,Monsudi KF

    更新日期:2015-09-01 00:00:00

  • Experiences of pregnant adolescents--voices from Wakiso district, Uganda.

    abstract:BACKGROUND:In Uganda, morbidity and mortality among adolescent mothers and their children are high. Social factors behind this problem need to be better understood. OBJECTIVE:To explore problems that pregnant adolescents face in order to design appropriate policies and interventions. METHODS:This was a descriptive st...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.5555/afhs.2005.5.4.304

    authors: Atuyambe L,Mirembe F,Johansson A,Kirumira EK,Faxelid E

    更新日期:2005-12-01 00:00:00

  • Attitude of married women towards contraceptive use in Ilorin Metropolis, Kwara State, Nigeria.

    abstract:Background:Contraceptive use helps in preventing unplanned pregnancy and reducing maternal death among married women. Objective:To investigate the attitude of married women towards contraceptive use in Ilorin metropolis, Kwara State. The study also examined whether the variables of age and educational attainment would...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v19i2.10

    authors: Adegboyega LO

    更新日期:2019-06-01 00:00:00

  • Comparison of Pulmonary TB DOTS clinic medication before and after the introduction of daily DOTS treatment and attitudes of treatment defaulters in the Western Division of the Gambia.

    abstract:BACKGROUND:Over a third of the world's population is infected with the tuberculin bacteria. In 1993 WHO launched the DOTS-strategy to enhance treatment compliance. Despite Gambia's adaption in 1985 it falls below WHO target of 85% cure rate. The defaulter rate was 14% in 2001, which reached 16% in the urban areas of th...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:

    authors: Sanneh AF,Pollock JI

    更新日期:2010-06-01 00:00:00

  • Analysis of personality traits as a risk factor in crash related trauma.

    abstract:BACKGROUND:Due to increasing stress, individual personality traits are becoming a significant contributor to CRT (Crash Related Trauma). In the present study, we hypothesized that there will be no difference in personality characteristics of CRT patients and control subjects and there will be no association between tra...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v16i3.27

    authors: Kumar V,Goyal R,Singh A,Sharma V,Srivastava RN,Kumar S,Kumar A

    更新日期:2016-09-01 00:00:00

  • Implementation of POCT in the diabetic clinic in a large hospital.

    abstract:AIM:Point-of-care testing (POCT) is gaining renewed interest, especially in resource-limiting primary health care, due to rise in prevalence of communicable and non-communicable diseases hence POCT needscontinuous appraisal. METHODS:Random glucose and glycated haemoglobin (HbA1c) were measured in 104 diabetic patients...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v15i3.26

    authors: Tanyanyiwa D,Dandara C,Bhana SA,Pauly B,Marule F,Ramokoka M,Bwititi P,Nwose U,Nkosi B

    更新日期:2015-09-01 00:00:00

  • Colistin resistance in Acinetobacter baumannii isolated from critically ill patients: clinical characteristics, antimicrobial susceptibility and outcome.

    abstract:Background:Acinetobacter baumannii (AB) is increasingly becoming a clinically relevant organism due to the rising number of associated nosocomial infections. The therapeutic options are extremely minimal because of its ability to develop resistance to all available antimicrobials, including colistin (CST). Data on the ...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v19i3.13

    authors: Elham B,Fawzia A

    更新日期:2019-09-01 00:00:00

  • Intestinal ameliorative effects of traditional Ogi-tutu, Vernonia amygdalina and Psidium guajava in mice infected with Vibrio cholera.

    abstract:BACKGROUND:Cholera, a severe acute watery diarrhea caused by Vibrio cholerae is endemic in Nigeria with most cases occurring in the rural areas. In South West Nigeria, some individuals resort to alternative treatments such as Ogi-tutu, Psidium guajava and Vernonia amygdalina during infections. The effectiveness of thes...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v16i2.33

    authors: Shittu OB,Ajayi OL,Bankole SO,Popoola TO

    更新日期:2016-06-01 00:00:00

  • Breastfeeding skills of full-term newborns and associated factors in a low-and-middle-income setting.

    abstract:Background:Normative information on the breastfeeding of term newborns may guide clinicians in early identification of breastfeeding difficulties and oro-pharyngeal dysphagia (OPD), and may support optimal breastfeeding practices. Objective:To describe breastfeeding skills of term newborn infants in a South African ho...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v19i3.43

    authors: Krüger EE,Kritzinger AA,Pottas LL

    更新日期:2019-09-01 00:00:00

  • Sero-prevalence and associated factors of Helicobacter pylori infection among adult patients with dyspepsia attending the gastroenterology unit in a tertiary hospital in Mwanza, Tanzania.

    abstract:BACKGROUND:Prevalence of H.pylori infection varies greatly between populations in different countries. This study was conducted to determine the magnitude of H.pylori among adult patients with dyspepsia attending the gastroenterology unit at Bugando medical centre. METHODS:A cross sectional study involving 202 dyspept...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v16i3.7

    authors: Jaka H,Mushi MF,Mirambo MM,Wilson L,Seni J,Mtebe M,Mshana SE

    更新日期:2016-09-01 00:00:00

  • Adverse drug reactions in patients admitted on internal medicine wards in a district and regional hospital in Uganda.

    abstract:INTRODUCTION:The burden of both community and hospital acquired adverse drug reactions (ADRs) are some of the important issues in pharmacotherapy. At the time of this study there was very scanty literature in this area from Africa. OBJECTIVE:This study was done to determine the frequency and characteristics of ADRs in...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:

    authors: Tumwikirize WA,Ogwal-Okeng JW,Vernby A,Anokbonggo WW,Gustafsson LL,Lundborg SC

    更新日期:2011-03-01 00:00:00

  • Is there any relationship between RDW levels and atrial fibrillation in hypertensive patient?

    abstract:BACKGROUND:Atrial fibrillation (AF) is the most common cardiac arrhythmia and increases the risk of stroke and death. Patients with hypertensive have an increased risk of developing atrial fibrillation. RDW (Red blood cell distribution width) levels are elevated in cardiovascular disorders including heart failure, stab...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v14i1.41

    authors: Sarikaya S,Şahin Ş,Akyol L,Börekçi E,Yilmaz YK,Altunkaş F,Karaman K

    更新日期:2014-03-01 00:00:00

  • Breast cancer resistance protein (BCRP) gene expression in a cohort of adult Egyptian patients with acute myeloid leukemia.

    abstract:Background:Acute myeloid leukemia (AML), an aggressive clonal disease, is genetically heterozygous. The prognostic role of expression of Breast Cancer Resistance Protein (BCRP) gene, which behaves as a multidrug transporter, in adult AML is ambiguous. Objective:The objective is to assess the level of mRNA expression o...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v18i4.15

    authors: El-Masry MW,Gouda HM,Shaheen IA,Edesa W,Hassan NM,Ramzy R

    更新日期:2018-12-01 00:00:00

  • Case report of a 26 year old primigravida with patent ductus arteriosus (PDA) in heart failure.

    abstract::Congenital heart disease is an important cause of maternal morbidity and mortality during pregnancy. Pregnancy alters the circulatory and respiratory physiology with attendant deleterious effect on the mother with congenital heart disease and the foetus. Additional insult to the circulatory physiology by other factors...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:

    authors: Akintunde AA,Opadijo OG

    更新日期:2011-03-01 00:00:00

  • Hemorrhagic fever in Saudi Arabia: challenge to public health, effective management and future considerations.

    abstract:Background:Viral hemorrhagic fevers (VHF) refers to a group of febrile illnesses caused by different viruses that result in high mortality in animals and humans. Many risk factors like increased human-animal interactions, climate change, increased mobility of people and limited diagnostic facility have contributed to t...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v20i3.17

    authors: Kumosani TA,Al-Malki AL,Razvi SS,Balgoon MJ,Kaleem M,Huwait EA,Alghamdi MA,Yaghmoor SS,Abualnaja KO,Barbour EK,Al-Madani KA,AlToukhi MH,Kumosani AT,Moselhy SS

    更新日期:2020-09-01 00:00:00

  • Knowledge, sexual behaviours and risk perception of sexually transmitted infections among students of the polytechnic, Ibadan, Oyo state.

    abstract:Background:Current evidence shows that the surge of sexually transmitted infections (STIs) is of public health importance. In Nigeria, about 3 million cases of STIs are reported annually with the highest rates observed among young people. Objective:This study was designed to investigate the knowledge, sexual behaviour...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v20i1.7

    authors: Oharume IM

    更新日期:2020-03-01 00:00:00

  • Recombinant human endostatin reduces hypertrophic scar formation in rabbit ear model through down-regulation of VEGF and TIMP-1.

    abstract:BACKGROUND:Recombinant human endostatin (Endostar) has been widely used to suppress angiogenesis in carcinoma patients. Hypertrophic scar (HS) tissue, much like a carcinoma, is often associated with angiogenesis. However, there have been few studies conducted on the effects of Endostar on HS or its mechanism. OBJECTIV...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v16i2.23

    authors: Wang P,Jiang LZ,Xue B

    更新日期:2016-06-01 00:00:00

  • Maternal nutrition: how is Eastern and Southern Africa faring and what needs to be done?

    abstract:BACKGROUND:The progress in key maternal health indicators in the Eastern and Southern Africa Region (ESAR) over the past two decades has been slow. OBJECTIVE:This paper analyzed available information on nutrition programs and nutrition-specific interventions targeting maternal nutrition in the ESAR and proposes steps ...

    journal_title:African health sciences

    pub_type: 杂志文章,meta分析

    doi:10.4314/ahs.v15i2.28

    authors: Salam RA,Syed B,Syed S,Das JK,Zagre NM,Rayco-Solon P,Bhutta ZA

    更新日期:2015-06-01 00:00:00

  • Effect of nutrient intakes on anthropometric profiles among university students from a selected private University in Klang Valley, Malaysia.

    abstract:Background:Dietary composition is the cornerstone of weight management. This study aimed to investigate the effect of nutrient intakes on anthropometric profiles among university students. Methods:This cross-sectional study was conducted among 411 students aged 18-29 years, purposive sampled from a selected private un...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v19i2.50

    authors: Koo HC,Hadirah Z,Airina A,Nurul Alifatul Amrina R,Faziela N

    更新日期:2019-06-01 00:00:00

  • Evaluation of Crimean-Congo hemorrhagic fever suspected cases admitted to a secondary care hospital in Kastamonu, Turkey between 2014-2017.

    abstract:Background:Crimean-Congo hemorrhagic fever (CCHF) is an endemic zoonosis in Kastamonu, Turkey. Clinical and laboratory findings may not be specific in the early phase of the disease, hence bringing a challenge to the clinician. Objective:We aimed to distinguish CCHF cases among all suspected cases by comparing them wi...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v19i1.16

    authors: Gozdas HT

    更新日期:2019-03-01 00:00:00

  • Ergogenic effect of varied doses of coffee-caffeine on maximal aerobic power of young African subjects.

    abstract:BACKGROUND:Caffeine one of the readily available stimulants consumed daily by more than 80% of the world's population, making it the most widely consumed drug in history. The objective of this study was to determine the effects of different doses (5, 10 &15 mg.kg(-1)) of caffeine per kilogram body weight on maximal aer...

    journal_title:African health sciences

    pub_type: 杂志文章,随机对照试验

    doi:

    authors: Lamina S,Musa DI

    更新日期:2009-12-01 00:00:00

  • Relationship between plasma homocysteine and blood pressure in hypertensive Northern-Nigerians.

    abstract:Aim:The study sought to determine whether there is any relationship between plasma homocysteine and blood pressure levels in Nigerians with essential hypertension. Method:It was a cross-sectional analytical study done on 120 randomly selected hypertensive patients and 120 normal healthy controls seen at the large Conf...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v20i1.38

    authors: Onyemelukwe OU,Maiha BB

    更新日期:2020-03-01 00:00:00

  • Neurosyphilis masquerading as hemiparesis and Jacksonian epilepsy in an HIV positive patient: a case report.

    abstract:BACKGROUND:Neurosyphilis is a common but often missed disease worldwide, mainly because it has many manifestations making its diagnosis difficult. It is often missed among HIV patients as the search for other co-infections such as toxoplasmosis often overshadows its consideration. OBJECTIVES:To describe one of our cas...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:

    authors: Kyebambe PS

    更新日期:2010-06-01 00:00:00

  • Skilled delivery care service utilization in Ethiopia: analysis of rural-urban differentials based on national demographic and health survey (DHS) data.

    abstract:BACKGROUND:Despite the slight progress made on Antenatal Care (ANC) utilization, skilled delivery care service utilization in Ethiopia is still far-below any acceptable standards. Only 10% of women receive assistance from skilled birth attendants either at home or at health institutions, and as a result the country is ...

    journal_title:African health sciences

    pub_type: 杂志文章

    doi:10.4314/ahs.v14i4.29

    authors: Fekadu M,Regassa N

    更新日期:2014-12-01 00:00:00