Abstract:
:Conventional influenza vaccines need to be designed and manufactured yearly. However, they occasionally provide poor protection owing to antigenic mismatch. Hence, there is an urgent need to develop universal vaccines against influenza virus. Using nucleoprotein (NP) and extracellular domain of matrix protein 2 (M2e) genes from the influenza A virus A/Beijing/30/95 (H3N2), we constructed four recombinant vaccinia virus-based influenza vaccines carrying NP fused with one or four copies of M2e genes in different orders. The recombinant vaccinia viruses were used to immunize BALB/C mice. Humoral and cellular responses were measured, and then the immunized mice were challenged with the influenza A virus A/Puerto Rico/8/34 (PR8). NP-specific humoral response was elicited in mice immunized with recombinant vaccinia viruses carrying full-length NP, while robust M2e-specific humoral response was elicited only in the mice immunized with recombinant vaccinia viruses carrying multiple copies of M2e. All recombinant viruses elicited NP- and M2e-specific cellular immune responses in mice. Only immunization with RVJ-4M2eNP induced remarkably higher levels of IL-2 and IL-10 cytokines specific to M2e. Furthermore, RVJ-4M2eNP immunization provided the highest cross-protection in mice challenged with 20 MLD50 of PR8. Therefore, the cross-protection potentially correlates with both NP and M2e-specific humoral and cellular immune responses induced by RVJ-4M2eNP, which expresses a fusion antigen of full-length NP preceded by four M2e repeats. These results suggest that the rational fusion of NP and multiple M2e antigens is critical toward inducing protective immune responses, and the 4M2eNP fusion antigen may be employed to develop a universal influenza vaccine.
journal_name
Virol Sinjournal_title
Virologica Sinicaauthors
Wang W,Huang B,Wang X,Tan W,Ruan Ldoi
10.1007/s12250-019-00138-9subject
Has Abstractpub_date
2019-10-01 00:00:00pages
583-591issue
5eissn
1674-0769issn
1995-820Xpii
10.1007/s12250-019-00138-9journal_volume
34pub_type
杂志文章abstract::Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the n...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3239-8
更新日期:2012-06-01 00:00:00
abstract::Human cytomegalovirus (HCMV), a herpesvirus, is an important human pathogen that causes asymptomatic infections in healthy or immunocompetent individuals but can lead to severe and potentially life-threatening complications in immune-immature individuals such as neonates or immune-compromised patients such as organ-tr...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-017-4055-y
更新日期:2017-10-01 00:00:00
abstract::The aim of the work is the comparison of the epidemiology of influenza and acute respiratory virus infections (ARVI) in the Republic of Kazakhstan with the corresponding influenza epidemic in Russia induced by influenza pandemic virus A/California/07/2009 in 2009. Data on influenza and ARVI from the Republic of Kazakh...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3203-z
更新日期:2011-10-01 00:00:00
abstract::The 5'-cap structures of eukaryotic mRNAs are important for RNA stability, pre-mRNA splicing, mRNA export, and protein translation. Many viruses have evolved mechanisms for generating their own cap structures with methylation at the N7 position of the capped guanine and the ribose 2'-Oposition of the first nucleotide,...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-016-3726-4
更新日期:2016-02-01 00:00:00
abstract::Ebola virus (EBOV) causes severe hemorrhagic fever in humans and non-human primates with high rates of fatality. Glycoprotein (GP) is the only envelope protein of EBOV, which may play a critical role in virus attachment and entry as well as stimulating host protective immune responses. However, the lack of expression ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-016-3845-y
更新日期:2016-12-01 00:00:00
abstract::Shiga toxin B-subunit (STxB) from Shigella dysenteriae targets in vivo antigen to cancer cells, dendritic cells (DC) and B cells, which preferentially express the globotriaosylceramide (Gb3) receptor. This pivotal role has encouraged scientists to investigate fusing STxB with other clinical antigens. Due to the challe...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3286-9
更新日期:2013-02-01 00:00:00
abstract::In this study, the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141-160 (epitope1), tandem repeat 200-213 (epitope2 (+2)) and the combination of two epitopes (epitope1-2) was genetically cloned into the prokaryotic expression vector pP...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-010-3041-4
更新日期:2010-02-01 00:00:00
abstract::Ion channels are membrane proteins that are found in a number of viruses and which are of crucial physiological importance in the viral life cycle. They have one common feature in that their action mode involves a change of electrochemical or proton gradient across the bilayer lipid membrane which modulates viral or c...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-010-3136-y
更新日期:2010-08-01 00:00:00
abstract::Ebola virus (EBOV) and Marburg virus (MARV), belonging to the Filoviridae family, emerged four decades ago and caused severe viral hemorrhagic fever in human and other primates. As high as 50-90% mortality, filoviruses can cause significant threats to public health. However, so far no specific and efficient vaccine ha...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-011-3186-9
更新日期:2011-04-01 00:00:00
abstract::Newcastle disease virus (NDV) and H9N2 subtype Avian influenza virus (AIV) are two notorious avian respiratory pathogens that cause great losses in the poultry industry. Current inactivated commercial vaccines against NDV and AIV have the disadvantages of inadequate mucosal responses, while an attenuated live vaccine ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00199-1
更新日期:2020-08-01 00:00:00
abstract::The virus receptors are key for the viral infection of host cells. Identification of the virus receptors is still challenging at present. Our previous study has shown that human virus receptor proteins have some unique features including high N-glycosylation level, high number of interaction partners and high expressi...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00259-6
更新日期:2020-07-28 00:00:00
abstract::Histone deacetylase (HDAC) inhibitors show clinical promise for the treatment of cancers, including hepatocellular carcinoma (HCC). In this study, we investigated the effect of HDAC inhibitor treatment on hepatitis C virus (HCV) replication in Huh7 human liver cells and in a mouse model of HCV infection. Viral replica...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0057-7
更新日期:2018-10-01 00:00:00
abstract::An exogenous avian leukosis virus (ALV) strain SDAU09C1 was isolated in DF-1 cells from one of 240 imported 1-day-old white meat-type grand parent breeder chicks. Inoculation of SDAU09C1 in ALV-free chickens induced antibody reactions specific to subgroup A or B. But gp85 amino acid sequence comparisons indicated that...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-010-3095-3
更新日期:2010-04-01 00:00:00
abstract::Conventional influenza vaccines are based on predicting the circulating viruses year by year, conferring limited effectiveness since the antigenicity of vaccine strains does not always match the circulating viruses. This necessitates development of universal influenza vaccines that provide broader and lasting protecti...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-020-00283-6
更新日期:2020-09-01 00:00:00
abstract::Our previous work has shown that Saffold virus (SAFV) induced several rodent and primate cell lines to undergo apoptosis (Xu et al. in Emerg Microb Infect 3:1-8, 2014), but the essential viral proteins of SAFV involved in apoptotic activity lack study. In this study, we individually transfected the viral proteins of S...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-019-00116-1
更新日期:2019-06-01 00:00:00
abstract::Bat SARS-like coronavirus (SL-CoV) has a genome organization almost identical to that of SARS-CoV, but the N-terminus of the Spike (S) proteins, which interacts with host receptor and is a major target of neutralizing antibodies against CoVs, of the two viruses has only 63-64% sequence identity. Although there have be...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3292-y
更新日期:2013-04-01 00:00:00
abstract::Circular RNAs (circRNAs) belong to a class of non-coding RNAs with diverse biological functions. However, little is known about their roles in case of pseudorabies virus (PrV) infection. Here, we analyzed the expression profile of host circRNAs from a virulent PrV type II strain DX (PrV-DX) infected and an attenuated ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00255-w
更新日期:2020-07-02 00:00:00
abstract::Human endogenous retrovirus W env (HERV-W env) plays a critical role in many neuropsychological diseases such as schizophrenia and multiple sclerosis (MS). These diseases are accompanied by immunological reactions in the central nervous system (CNS). Microglia are important immunocytes in brain inflammation that can p...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-017-3997-4
更新日期:2017-06-01 00:00:00
abstract::Hepatitis B virus (HBV) infection is still a worldwide health problem; however, the current antiviral therapies for chronic hepatitis B are limited in efficacy. The outcome of HBV infection is thought to be the result of complex interactions between the HBV and the host immune system. While the role of the adaptive im...
journal_title:Virologica Sinica
pub_type: 杂志文章,评审
doi:10.1007/s12250-014-3412-3
更新日期:2014-02-01 00:00:00
abstract::Recreational waters contaminated with human fecal pollution are a public health concern, and ensuring the safety of recreational waters for public use is a priority of both the Environmental Protection Agency (EPA) and the Centers for Disease Control and Prevention (CDC). Current recreational water standards rely on f...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-015-3644-x
更新日期:2015-10-01 00:00:00
abstract::Similar to Hepatitis C virus (HCV) infection in humans, HCVcc infection can also result in persistent and chronic infection. The core protein is a variable protein and exists in several sizes. Some sizes of core proteins have been reported to be related to chronic HCV infection. To study the possible role of the core ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-013-3296-7
更新日期:2013-06-01 00:00:00
abstract::Hepatitis C virus (HCV) is a global health concern, notably in Southeast Asia, and in Laos the presentation of the HCV-induced liver disease is poorly known. Our objective was thus to describe a comprehensive HCV infection pattern in order to guide national health policies. A study on a group of 1765 patients formerly...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-018-0039-9
更新日期:2018-08-01 00:00:00
abstract::During virus infection, RIG-I-like receptors (RLRs) recognize viral RNAs and recruit the adaptor protein VISA to activate downstream signaling, leading to activation of transcription factors NF-κB and IRF3, which collaborate to induce type I interferons (IFNs). IFNs further induce expression of hundreds of IFN-stimula...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00267-6
更新日期:2020-08-07 00:00:00
abstract::A 62-year-old man had chronic hepatitis B virus (HBV) infection and was diagnosed with liver cirrhosis. At the time of diagnosis the patient's virologic markers were positive for hepatitis B surface antigen (HBsAg), antibody to hepatitis B e antigen (anti-HBe) and antibody to hepatitis B core antigen (anti-HBc), while...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3284-3
更新日期:2012-12-01 00:00:00
abstract::Severe fever with thrombocytopenia syndrome virus (SFTSV), the causative agent of a febrile human disease, was first identified from central and eastern provinces in China, and later in Japan and South Korea. Hubei Province is one of the major SFTS epidemic areas in the central part of China. This study reported the i...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00289-0
更新日期:2020-09-22 00:00:00
abstract::Oncolytic alphavirus M1 has been shown to selectively target and kill cancer cells, but cytopathic morphologies induced by M1 virus and the life cycle of the M1 strain in cancer cells remain unclear. Here, we study the key stages of M1 virus infection and replication in the M1 virus-sensitive HepG2 liver cancer cell l...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00339-7
更新日期:2021-01-22 00:00:00
abstract::Receptors interaction protein 2 (RIP2) is a specific adaptor molecule in the downstream of NOD2. The role of RIP2 during foot-and-mouth disease virus (FMDV) infection remains unknown. Here, our results showed that RIP2 inhibited FMDV replication and played an important role in the activation of IFN-β and NF-ĸB signal ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00322-2
更新日期:2021-01-05 00:00:00
abstract::Laos is the only landlocked country in Southeast Asia and borders Thailand, Myanmar and Cambodia, the three countries in this region that have been hardest hit by human immunodeficiency virus (HIV). Laos has been regarded as a low-HIV-prevalence country for decades. To understand the status of HIV in Laos in recent ye...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-020-00215-4
更新日期:2020-10-01 00:00:00
abstract::Feasible and effective cell models for hepatitis B virus (HBV) infection are required for investigating the complete lifecycle of this virus, including the early steps of viral entry. Resistance to dimethyl sulfoxide/polyethylene glycol (DMSO/PEG), hNTCP expression, and a differentiated state are the limiting factors ...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-017-3983-x
更新日期:2017-12-01 00:00:00
abstract::In this study, the decay of maternal peste des petits ruminants virus (PPRV) antibodies in kids born to goats vaccinated with Asian lineage IV PPR vaccine and the efficacy of passive immunity against PPRV was assessed to determine the appropriate period for vaccination in kids. Serum samples collected from kids born t...
journal_title:Virologica Sinica
pub_type: 杂志文章
doi:10.1007/s12250-012-3249-6
更新日期:2012-08-01 00:00:00