mSphere of Influence: the Importance of Metabolism for Pathogen Adaptation to Host-Imposed Stresses.

abstract：:In fungi, most metabolic processes are subject to regulation by light. Trichoderma reesei is adapted to degradation of plant cell walls and regulates production of the required enzymes in a manner dependent on the nutrient source and the light status. Here we investigated the interrelated relevance of two regulation l...

journal_title：mSphere

authors： Stappler E,Dattenböck C,Tisch D,Schmoll M

更新日期：2017-05-10 00:00:00

abstract：:Human adenoviruses (HAdV), species D in particular (HAdV-D), are frequently associated with epidemic keratoconjunctivitis (EKC). Although the infection originates at the ocular surface epithelium, the mechanisms by which HAdV-Ds bypass the membrane-associated mucin (MAM)-rich glycocalyx of the ocular surface epitheliu...

journal_title：mSphere

authors： Menon BB,Zhou X,Spurr-Michaud S,Rajaiya J,Chodosh J,Gipson IK

更新日期：2016-02-10 00:00:00

abstract：:Bifidobacterium species are important commensals capable of dominating the infant gut microbiome, in part by producing acids that suppress growth of other taxa. Bifidobacterium species are less prone to possessing antimicrobial resistance (AMR) genes (ARGs) than other taxa that may colonize infants. Given that AMR is ...

journal_title：mSphere

authors： Taft DH,Liu J,Maldonado-Gomez MX,Akre S,Huda MN,Ahmad SM,Stephensen CB,Mills DA

更新日期：2018-09-26 00:00:00

abstract：:Replication forks must respond to changes in nutrient conditions, especially in bacterial cells. By investigating the single-molecule dynamics of replicative helicase DnaC, DNA primase DnaG, and lagging-strand polymerase DnaE in the model bacterium Bacillus subtilis, we show that proteins react differently to stress c...

journal_title：mSphere

authors： Hernández-Tamayo R,Schmitz H,Graumann PL

更新日期：2021-01-27 00:00:00

abstract：:Nonribosomal peptide synthases produce short peptides in a manner that is distinct from classical mRNA-dependent ribosome-mediated translation. The Mycobacterium tuberculosis genome harbors a nonribosomal peptide synthase gene, nrp, which is part of a gene cluster proposed to be involved in the biosynthesis of isonitr...

journal_title：mSphere

authors： Bhatt K,Machado H,Osório NS,Sousa J,Cardoso F,Magalhães C,Chen B,Chen M,Kim J,Singh A,Ferreira CM,Castro AG,Torrado E,Jacobs WR Jr,Bhatt A,Saraiva M

更新日期：2018-10-31 00:00:00

abstract：:Many coronavirus disease 2019 (COVID-19) patients demonstrate lethal respiratory complications caused by cytokine release syndrome (CRS). Multiple cytokines have been implicated in CRS, but levels of tumor necrosis factor superfamily 14 (TNFSF14) (LIGHT) have not been previously measured in this setting. In this study...

journal_title：mSphere

authors： Perlin DS,Zafir-Lavie I,Roadcap L,Raines S,Ware CF,Neil GA

更新日期：2020-08-12 00:00:00

abstract：:Clostridioides difficile is an obligately anaerobic, spore-forming, Gram-positive pathogenic bacterium that is considered the leading cause of nosocomial diarrhea worldwide. Recent studies have attempted to understand the biology of the outermost layer of C. difficile spores, the exosporium, which is believed to contr...

journal_title：mSphere

authors： Romero-Rodríguez A,Troncoso-Cotal S,Guerrero-Araya E,Paredes-Sabja D

更新日期：2020-11-18 00:00:00

abstract：:Hypervirulent Klebsiella pneumoniae (hvKp) is an emerging pathogen of increasing concern due to its ability to cause serious organ and life-threatening infections in healthy individuals and its increasing acquisition of antimicrobial resistance determinants. Identification of hvKp is critical for patient care and epid...

journal_title：mSphere

authors： Russo TA,MacDonald U

更新日期：2020-01-08 00:00:00

abstract：:Microsporidia are obligate intracellular parasites that infect a wide variety of host organisms, including humans. The sporoplasm is the initial stage of microsporidian infection and proliferation, but its morphological and molecular characteristics are poorly understood. In this study, the sporoplasm of Nosema bombyc...

journal_title：mSphere

authors： He Q,Luo J,Xu JZ,Wang CX,Meng XZ,Pan GQ,Li T,Zhou ZY

更新日期：2020-02-12 00:00:00

abstract：:Current in vitro techniques for the culture of microorganisms, and particularly of delicate microbial biofilms, are still mostly limited to low-density plates and manual labor and are not amenable to automation and true high-throughput (HT) applications. We have developed a novel fully automated platform for the forma...

journal_title：mSphere

authors： Srinivasan A,Torres NS,Leung KP,Lopez-Ribot JL,Ramasubramanian AK

更新日期：2017-06-28 00:00:00

abstract：:Influenza D virus (IDV) is unique among four types of influenza viruses in that it utilizes cattle as a primary reservoir. The thermal and acid stability of IDV were examined and directly compared with those of influenza A virus (IAV), influenza B virus (IBV), and influenza C virus (ICV). The results of our experiment...

journal_title：mSphere

authors： Yu J,Hika B,Liu R,Sheng Z,Hause BM,Li F,Wang D

更新日期：2017-08-09 00:00:00

abstract：:The extraintestinal pathogenic Escherichia coli (ExPEC) H30 subclone of sequence type 131 (ST131-H30) has emerged abruptly as a dominant lineage of ExPEC responsible for human disease. The ST131-H30 lineage has been well described phylogenetically, yet its plasmid complement is not fully understood. Here, single-molec...

journal_title：mSphere

authors： Johnson TJ,Danzeisen JL,Youmans B,Case K,Llop K,Munoz-Aguayo J,Flores-Figueroa C,Aziz M,Stoesser N,Sokurenko E,Price LB,Johnson JR

更新日期：2016-06-29 00:00:00

abstract：:Commensal and generally harmless in healthy individuals, Enterococcus faecalis causes opportunistic infections in immunocompromised patients. Plasmid-cured E. faecalis strain VE14089, derived from sequenced reference strain V583, is widely used for functional studies due to its improved genetic amenability. Although s...

journal_title：mSphere

authors： Furlan S,Matos RC,Kennedy SP,Doublet B,Serror P,Rigottier-Gois L

更新日期：2019-07-10 00:00:00

abstract：:Coxsackievirus typically infects humans via the gastrointestinal tract, which has a large number of microorganisms collectively referred to as the microbiota. To study how the intestinal microbiota influences enteric virus infection, several groups have used an antibiotic regimen in mice to deplete bacteria. These stu...

journal_title：mSphere

authors： Woods Acevedo MA,Erickson AK,Pfeiffer JK

更新日期：2019-02-20 00:00:00

abstract：:The chikungunya virus (CHIKV) is transmitted by female Aedes aegypti and Aedes albopictus mosquitoes, mostly present in (sub)tropical regions. No antivirals are available to treat CHIKV infections. If antiviral drugs are proven efficient to treat CHIKV-infected patients, it will be pivotal to determine whether drug-re...

journal_title：mSphere

authors： Delang L,Yen PS,Vallet T,Vazeille M,Vignuzzi M,Failloux AB

更新日期：2018-08-22 00:00:00

abstract：:Molecular detection of biological agents in the field has traditionally relied on the use of quantitative real-time PCR (qPCR), which now includes commercially available instruments that can be used in the laboratory or field. Adapting this technology for field-forward applications necessitated innovation to minimize ...

journal_title：mSphere

authors： Yeh KB,Wood H,Scullion M,Russell JA,Parker K,Gnade BT,Jones AR,Whittier C,Mereish K

更新日期：2019-12-11 00:00:00

abstract：:Cryptococcus gattii R265 is a hypervirulent fungal strain responsible for the recent outbreak of cryptococcosis in Vancouver Island of British Columbia in Canada. It differs significantly from Cryptococcus neoformans in its natural environment, its preferred site in the mammalian host, and its pathogenesis. Our previo...

journal_title：mSphere

authors： Lam WC,Upadhya R,Specht CA,Ragsdale AE,Hole CR,Levitz SM,Lodge JK

更新日期：2019-10-09 00:00:00

abstract：:The oral periodontopathic bacterium Fusobacterium nucleatum has been repeatedly associated with colorectal tumors. Molecular analysis has identified specific virulence factors that promote tumorigenesis in the colon. However, other oral community members, such as members of the Porphyromonas spp., are also found with ...

journal_title：mSphere

authors： Flynn KJ,Baxter NT,Schloss PD

更新日期：2016-05-11 00:00:00

abstract：:Infections by malaria parasites can lead to very different clinical outcomes, ranging from mild symptoms to death. Differences in the ability of the spleen to deal with the infected red blood cells (iRBCs) are linked to differences in virulence. Using virulent and avirulent strains of the rodent malaria parasite Plasm...

journal_title：mSphere

authors： Huang X,Huang S,Ong LC,Lim JC,Hurst RJ,Mushunje AT,Matsudaira PT,Han J,Preiser PR

更新日期：2015-12-09 00:00:00

abstract：:Export of parasite proteins into the host erythrocyte is essential for survival of Plasmodium falciparum during its asexual life cycle. While several studies described key factors within the parasite that are involved in protein export, the mechanisms employed to traffic exported proteins within the host cell are curr...

journal_title：mSphere

authors： Cobb DW,Florentin A,Fierro MA,Krakowiak M,Moore JM,Muralidharan V

更新日期：2017-09-27 00:00:00

abstract：:This study examines the pharmacodynamics of antimicrobials that are used to treat Salmonella with each other and with key components of the innate immune system. Antimicrobial synergy was assessed using time-kill and checkerboard assays. Antimicrobial interactions with innate immunity were studied by employing catheli...

journal_title：mSphere

authors： Sakoulas G,Kumaraswamy M,Kousha A,Nizet V

更新日期：2017-12-06 00:00:00

abstract：:The cell-to-cell heterogeneity in a bacterial population provides a rich response to environmental changes and robust survival of an isogenic population. Especially, the rare, extreme phenotypes can be important for survival under transient lethal conditions. We analyze the probability of having an extremely high or l...

journal_title：mSphere

authors： Svenningsen MS,Semsey S,Mitarai N

更新日期：2019-01-30 00:00:00

abstract：:Immunity to Toxoplasma gondii at early stages of infection in C57BL/6 mice depends on gamma interferon (IFN-γ) production by NK cells, while at later stages it is primarily mediated by CD8 T cells. We decided to explore the requirement for CD4 T cells during T. gondii infection in Batf3-/- mice, which lack CD8α+ dendr...

journal_title：mSphere

authors： Tussiwand R,Behnke MS,Kretzer NM,Grajales-Reyes GE,Murphy TL,Schreiber RD,Murphy KM,Sibley LD

更新日期：2020-07-15 00:00:00

abstract：:Candida auris, an emerging multidrug-resistant yeast, has recently been associated with outbreaks of invasive infections in health care facilities worldwide. Its success as a nosocomial pathogen lies in its capability to sustain for prolonged periods in the intensive care unit (ICU), adeptly colonize skin, and spread ...

journal_title：mSphere

authors： Uppuluri P

更新日期：2020-01-22 00:00:00

abstract：:The acquisition of genetic material conferring the arsenal necessary for host virulence is a prerequisite on the path to becoming a plant pathogen. More subtle mutations are also required for the perception of cues signifying the presence of the target host and optimal conditions for colonization. The decision to acti...

journal_title：mSphere

authors： Jourdan S,Francis IM,Deflandre B,Loria R,Rigali S

更新日期：2017-03-01 00:00:00

abstract：:Outbreaks of infection occur more often than they are reported in most developing countries, largely due to poor diagnostic services. A Klebsiella species bacteremia outbreak in a newborn unit with high mortality was recently encountered at a location being surveilled for childhood bacteremia. These surveillance effor...

journal_title：mSphere

authors： Brinkac LM,White R,D'Souza R,Nguyen K,Obaro SK,Fouts DE

更新日期：2019-03-13 00:00:00

abstract：:Skin and soft tissue infections (SSTIs) are common in the general population, with increased prevalence among military trainees. Previous research has revealed numerous nasal microbial signatures that correlate with SSTI development and Staphylococcus aureus colonization. Thus, we hypothesized that the ecology of the ...

journal_title：mSphere

authors： Singh J,Johnson RC,Schlett CD,Elassal EM,Crawford KB,Mor D,Lanier JB,Law NN,Walters WA,Teneza-Mora N,Bennett JW,Hall ER,Millar EV,Ellis MW,Merrell DS

更新日期：2016-10-05 00:00:00

abstract：:Vibrio cholerae is a noninvasive pathogen that colonizes the small intestine and produces cholera toxin, causing severe secretory diarrhea. Cholera results in long lasting immunity, and recent studies have improved our understanding of the antigenic repertoire of V. cholerae Interactions between the host, V. cholerae,...

journal_title：mSphere

authors： Weil AA,Becker RL,Harris JB

更新日期：2019-11-27 00:00:00

abstract：:Grf10, a homeodomain-containing transcription factor, regulates adenylate and one-carbon metabolism and morphogenesis in the human fungal pathogen Candida albicans Here, we identified functional domains and key residues involved in transcription factor activity using one-hybrid and mutational analyses. We localized ac...

journal_title：mSphere

authors： Wangsanut T,Tobin JM,Rolfes RJ

更新日期：2018-10-24 00:00:00

abstract：:Clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 genome modification systems have greatly facilitated the genetic analysis of fungal pathogens. In CRISPR-Cas9 genome editing methods designed for use in Candida albicans, DNAs that encode the necessary components are expressed in the target cells. ...

journal_title：mSphere

authors： Grahl N,Demers EG,Crocker AW,Hogan DA

更新日期：2017-06-21 00:00:00