Abstract:
:Tumours of astroglial origin, both malignant glioblastoma (GBM) and benign subependymal giant cell astrocytoma (SEGA), pose a serious medical problem. Casein kinase 2 (CK2), a member of the serine/threonine kinase family, has antiapoptotic properties and plays a vital role in glial tumour cell survival. It contributes to invasive cell growth and is often upregulated in malignant neoplastic cells; however, its role in benign tumours of astrocytic origin is less understood. In the present study we investigated the effects of small molecule CK2 inhibitors on proliferation and viability of glioma cells in vitro. The experiments were conducted on commercial T98G malignant glioma cell line and the SEGA cell line, derived from a paediatric case of tuberous sclerosis complex (TSC). Cell cultures were incubated with selected CK2 inhibitors: 4,5,6,7-tetrabromo-1H-benzimidazole (TBI), 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole (DMAT) and 4,5,6,7-tetrabromo-1H-benzotriazole (TBB) at 0.1, 1, 10, 25, 50, 75 and 100 µM concentrations for 24 and 48 hours. Cell proliferation was assessed using a cell counter and cell viability was evaluated by MTT assay. TBB at 75 µM and 100 µM, and TBI starting from 25 µM, both reduced T98G cell proliferation after 24 hours, while DMAT was ineffective. All tested small molecule CK2 inhibitors appear to reduce T98G cell growth and viability after 48 hours, although TBI appeared to be the most effective and reduced cell growth in the 50-100 µM dose range. TBI also showed potential efficacy in reducing the number and viability of SEGA cells after 48 hours. Proliferation and viability of SEGA cells have proven resistant to TBB treatment. DMAT only reduced the viability of SEGA cells at 24 (at 100 µM) and 48 hours (10-100 µM). Importantly, normal human astrocyte cells were found to be moderately resistant to TBB, while their viability was mildly reduced at higher doses of DMAT and TBI. In conclusion, CK2 appears to play a role not only in malignant glioma cells but it can also sustain the viability and proliferation of benign astrocytoma cells. The obtained antitumor effects of CK2 inhibitors significantly exceeded their mild or no effect on normal astrocytes in control, which supports the therapeutic potential of these compounds against gliomas.
journal_name
Folia Neuropatholjournal_title
Folia neuropathologicaauthors
Pucko E,Ostrowski R,Matyja Edoi
10.5114/fn.2019.88452subject
Has Abstractpub_date
2019-01-01 00:00:00pages
239-248issue
3eissn
1641-4640issn
1509-572Xpii
37901journal_volume
57pub_type
杂志文章abstract::The investigations were based on 3 cases with Leigh, 5 cases with Krabbe's, 4 cases of Alpers, 2 cases with Sandhoff, 1 case with Alexander's disease and 1 case with metachromatic leukodystrophy. In 1 case included into the study we have diagnosed nonketotic hyperglycinemia II. All the diseases under examination are r...
journal_title:Folia neuropathologica
pub_type: 杂志文章,评审
doi:
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:The efficacy of tempol and its acyl derivative tempol-C8 as retinoprotective agents was compared in a rat model of NMDA-induced retinal ganglion cell (RGC) damage. MATERIAL AND METHODS:Tempol or tempol-C8 in different doses was administered intraperitoneally to 6 weeks old (pre-adolescent) and 9-10 weeks ol...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:10.5114/fn.2014.45570
更新日期:2014-01-01 00:00:00
abstract::Some data suggest that the central nervous system (CNS) is the main target of Staphylococcus alpha-toxin. Since this pathogen cannot penetrate the blood-brain barrier (BBB), the exact mechanism by which alpha-toxin affects the CNS remains unclear. Recent studies on the role of the innate immune system have shed light ...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::A contribution of B cells and autoantibodies has been demonstrated in MS leading to interest in the use of such autoantibodies as diagnostic or prognostic markers and as a basis for immunomodulatory therapy. ELISA and Western fail to detect reactivity against epitopes displayed by native antigens expressed on myelin s...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Metallothioneins (MT) are common proteins in animal tissues. These proteins take part in the homeostasis of the ions of the metals which are necessary for the proper metabolism of the organism (zinc, copper), biosynthesis regulation and zincprotein activity (for example the activity of the zinc-dependant transcription...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract:INTRODUCTION:Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary, progressive ischemic disease of small vessels of the brain characterized by migraine with aura (MA), recurrent subcortical ischemic episodes, cognitive decline and psychiatric disorders. CA...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:10.5114/fn.2020.94009
更新日期:2020-01-01 00:00:00
abstract::The pineal gland is an organ involved in regulation of homeostasis and body rhythms. It plays an important role in the growth foetuses and adaptation of newborns to new environmental conditions. The requirements of foetuses and newborns progressively change during development. The purpose of the study was to evaluate ...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Early reviews identified over 20 risk factors associated with Alzheimer's disease (AD) including age, familial inheritance, exposure to aluminium, traumatic brain injury (TBI), and associated co-morbidities such as vascular disease and infection. In the light of recent evidence, this review reconsiders these risk fact...
journal_title:Folia neuropathologica
pub_type: 杂志文章,评审
doi:10.5114/fn.2019.85929
更新日期:2019-01-01 00:00:00
abstract::The Warburg micro syndrome (WARBM) is a genetically heterogeneous syndrome linked to at least 4 loci. At the clinical level, WARBM is characterized by microcephaly, microphthalmia, microcornea, congenital cataracts, corpus callosum hypoplasia, severe mental retardation, and hypogonadism. In some families additional cl...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:10.5114/fn.2016.62537
更新日期:2016-01-01 00:00:00
abstract::Hyponatremia is a common disorder of water-electrolyte balance characterized by the decrease of plasma sodium ions concentration below 135 mM. Although water-electrolyte balance is regulated by a kidney, symptoms of hyponatremia are related to the disturbances of intracranial homeostasis and are attributed to brain sw...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:10.5114/fn.2013.37707
更新日期:2013-01-01 00:00:00
abstract::Neuroacanthocytosis is a rare disease of nervous system with multisystem pathology. This review presents clinical syndromes and morphological changes of sporadic and familial forms of neuroacanthocytosis and is illustrated by the case of a 27-year-old man. Progressive extrapyramidal syndrome appeared at the age of 22....
journal_title:Folia neuropathologica
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::The aim of the study was to analyse in TEM the evolution of changes in structural elements of synaptic junctions of the cerebellar cortex in rats in valproate encephalopathy induced by chronic 12-month administration of sodium valproate - VPA (once daily intragastrically, in a dose of 200 mg/kg b.w.) and after withdra...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The MCP-1 and RANTES levels were measured in 20 multiple sclerosis patients before and after 1 year daily treatment with 20 mg of subcutaneously applied glatiramer acetate. The level of MCP-1 in serum from multiple sclerosis patients was lower than in control subjects. After one year of therapy with glatiramer acetate...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The aim of our study was to present six cases with cystic changes within the white matter in infant brains and discuss the variants of this type of neuropathological lesions of the developing brain. Two of them exhibited the changes characteristic for cystic leucoencephalopathy. To the others with moderate involvement...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:1994-01-01 00:00:00
abstract:INTRODUCTION:Transsphenoidal surgery is the treatment of choice in Cushing's disease (CD), although even late recurrences occur in some patients. Low expression of O-6-methylguanine-DNA methyltransferase (MGMT) has been linked to a high risk of relapse in pituitary tumours, but the evidence for corticotroph adenomas is...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:10.5114/fn.2020.102438
更新日期:2020-01-01 00:00:00
abstract::The endothelial vacuolar and vesicular transports in traumatic human brain oedema have been reviewed and compared with experimental brain oedema in order to establish their role in both oedema formation and oedema resolution. Normal or "non-activated" and "activated" capillaries are found. The activated capillaries sh...
journal_title:Folia neuropathologica
pub_type: 杂志文章,评审
doi:10.5114/fn.2013.35951
更新日期:2013-01-01 00:00:00
abstract::The authors present a rare case of C-6 vertebral involvement in a 12-year-old boy with histiocytosis X. The patient presented with limitation in movements of neck and upper extremities. Computed tomography (CT) and magnetic resonance (MRI) imaging were used in the preoperative workup. Surgery was performed via an ante...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract:INTRODUCTION:To investigate the effects of crocin on proliferation and migration of endogenous neural stem cells and the Notch1 signalling pathway in rats after cerebral ischemia reperfusion. MATERIAL AND METHODS:SD rats were randomly divided into the sham operation group, model group and administration group (crocin)...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:10.5114/fn.2020.100063
更新日期:2020-01-01 00:00:00
abstract::Morphological alterations and the course of changes in motoneuron counts were studied by light microscopy (cresyl violet staining) in the L2/L3 region of the spinal cord of hemizygotic transgenic rats carrying the amyotrophic lateral sclerosis-associated mutant human gene for Cu,Zn superoxide dismutase (hSOD1G93A) and...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The subcellular distribution of glutamate and GABA in synapses of Mongolian gerbils hippocampus was examined using post-embedding immunogold staining method for electron microscopy. Immunolabelling was performed with 10 nm gold-antibody complexes for glutamate and GABA. The gold particle densities gave reliable inform...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::PPAX6 is an important transcription factor which plays an essential role in brain morphogenesis and eye development. It is related to migration of neuroblasts to the cerebral cortex and deep telencephalic nuclei, and the specification of cellular and regional identity. Disturbances of brain development in two sib fetu...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Globoid cell leukodystrophy (GLD, also known as Krabbe disease), whose pathophysiology is still not completely elucidated, is an inherited, metabolic, and neurodegenerative disease, caused by the deficiency of β-galactocerebrosidase (GALC) or in very rare cases by lack of active saposin A. We describe two patients, i...
journal_title:Folia neuropathologica
pub_type: 杂志文章,评审
doi:10.5114/fn.2012.32364
更新日期:2012-01-01 00:00:00
abstract::We demonstrate an unusually intensive accumulation of prion protein (PrP) on neuronal membranes in hamsters infected with the Echigo-1 panencephalopathic strain of Creutzfeldt-Jakob disease. This stands in strong contrast with the poor vacuolation in this model. Several distinct patterns of PrP(TSE) deposit were obser...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The effect of transient, global cerebral ischemia on the distribution of endothelin (ET) in blood-brain barrier (BBB) in CA1 area of hippocampus long-time after ischemia was estimated using post-embedding immunogold technique. ET-like immunoreactivity as a gold particles was localized in all compartments of the blood-...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Adenylosuccinase (ADSL) deficiency is an autosomal recessive disorder affecting mainly the nervous system. The disease causes psychomotor retardation, frequently with autistic features and epilepsy. ADSL deficiency may be diagnosed by detection of two abnormal metabolites in body fluids--succinyladenosine (S-Ado) and ...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::A neuropathological analysis of 172 cases of AIDS in adults was carried out, to determine the occurrence and nature of the opportunistic infections of the central nervous system (CNS). The material comprised 155 cases of men, and 17 women. Mean age of patients was 38 years. Collection under study originated from the p...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::Epithelioid schwannomas occur predominantly in relation to peripheral nerves and are associated with histological and clinical malignancy. However, a variant of the epithelioid schwannoma involving cranial nerves is extremely rare. In this study we report three cases of epithelioid schwannomas originating from the aco...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2000-01-01 00:00:00
abstract::Metallothioneins take part in the homeostasis of the ions of the metals which are necessary for the proper metabolism of the organism (zinc, copper), in biosynthesis regulation of the zinc-containing proteins and also in the detoxication of metals from the tissues. They also protect the tissue from the effects of free...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract:OBJECTIVES:Ischemic stroke remains one of the top ten causes of death in children. There is evidence for the role of pro-inflammatory cytokines, such as IL-6 and the -174G>C promoter polymorphism of the IL-6 gene, in the occurrence and outcome of stroke in adults. The aim of the present study was to determine a possibl...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::Multiple system atrophy (MSA) is a rare neurodegenerative disorder associated with parkinsonism, ataxia, and autonomic dysfunction. Its pathology is primarily subcortical comprising vacuolation, neuronal loss, gliosis, and α-synucleinimmunoreactive glial cytoplasmic inclusions (GCI). To quantify cerebellar pathology i...
journal_title:Folia neuropathologica
pub_type: 杂志文章
doi:10.5114/fn.2015.54420
更新日期:2015-01-01 00:00:00