DNA methylation data-based molecular subtype classification related to the prognosis of patients with cervical cancer.

Abstract:

:Cervical cancer is one of the leading female health-killers among all types of malignancies globally. Human papillomavirus infection combined with genetic and epigenetic alterations have been indicated to be closely associated with the pathogenesis, progression, and malignant transformation of cervical cancer. Notably, during the complex tumorigenesis process, a series of DNA methylations occurs early and is the most frequent molecular behavior. In this study, to exploit the specific DNA methylation sites influencing the prognosis of patients with cervical cancer, 275 samples were downloaded from The Cancer Genome Atlas database and further analyzed. As a result, 1253 CpGs were found to have a significant correlation with patient prognosis and were further selected for the consistent clustering of samples into six subgroups. Specifically, the samples in every subgroup were different regarding the following: race, age, tumor stage, receptor status, histological type, metastasis status, and patient prognosis. In addition, we calculated the levels of methylation sites in all subgroups, with 79 methylation sites (corresponding to 81 genes) screened as the intrasubgroup-specific methylation sites. Moreover, signaling pathway enrichment analysis was conducted on the genes of the corresponding promoter regions of the above-described specific methylation sites, revealing that these genes were enriched in biological pathways closely associated with tumors, such as the cyclic guanosine monophosphate-dependent protein kinase and focal adhesion signaling pathways. Finally, the least absolute shrinkage and selection operator algorithm was employed to establish a prognostic prediction model for cervical cancer patients, with training and test sets used for testing and validation, respectively. In summary, the specific DNA methylation site-based classification is able to reflect the heterogeneity of cervical cancer tissue, contributing to the development of personalized therapy and the accurate prediction of patient prognosis.

journal_name

J Cell Biochem

authors

Li C,Ke J,Liu J,Su J

doi

10.1002/jcb.29491

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

2713-2724

issue

3

eissn

0730-2312

issn

1097-4644

journal_volume

121

pub_type

杂志文章
  • Identification of novel prognostic alternative splicing signature in papillary renal cell carcinoma.

    abstract::Papillary renal cell carcinoma (pRCC) is a heterogeneous disease containing multifocal or solitary tumors with an aggressive phenotype. Increasing evidence has indicated the involvement of aberrant splicing variants in renal cell cancer, while systematic profiling of aberrant alternative splicing (AS) in pRCC was lack...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29314

    authors: Duan Y,Zhang D

    更新日期:2020-01-01 00:00:00

  • Differential expression of α6 and β1 integrins reveals epidermal heterogeneity at single-cell resolution.

    abstract::Skin epidermis is a constantly renewing epithelium that is composed of various cell types and provides an ideal model system for tissue regeneration and heterogeneity studies. Integrins are a family of transmembrane receptors that mediate cell adhesion in the epidermis, and integrin expression spatially reflects epide...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29487

    authors: He W,Ye J,Xu H,Lin Y,Zheng Y

    更新日期:2020-03-01 00:00:00

  • DACH1 negatively regulates the human RANK ligand gene expression in stromal/preosteoblast cells.

    abstract::Receptor activator of NF-kappaB ligand (RANKL) is a critical osteoclastogenic factor that is expressed on bone marrow stromal/preosteoblast cells. Most bone resorption stimuli induce osteoclast formation by modulating RANKL expression in these cells. However, little is known about the mechanisms regulating RANKL gene ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21561

    authors: Sundaram K,Mani SK,Kitatani K,Wu K,Pestell RG,Reddy SV

    更新日期:2008-04-15 00:00:00

  • Cell Cycle-Dependent Localization of Dynactin Subunit p150 glued at Centrosome.

    abstract::p150(glued) is the largest subunit of dynactin protein complex, through which cargo vesicles link to the microtubule minus-end directed motor protein dynein. In addition, p150(glued) also locates in the mother centriole where it organizes the subdistal appendage. The components of appendage are dynamically regulated t...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25160

    authors: Chen TY,Syu JS,Han TY,Cheng HL,Lu FI,Wang CY

    更新日期:2015-09-01 00:00:00

  • Effects of heat shock on the distribution and expression levels of nuclear proteins in HeLa S3 cells.

    abstract::Cumulating evidence has led to the idea that nuclear functions such as DNA replication, RNA transcription, RNA splicing and nucleocytoplasmic transport are facilitated by a proteinaceous architectural framework within the nuclear compartment and at the nuclear envelope. In the present study, we have used immunofluores...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.21968

    authors: Haddad N,Paulin-Levasseur M

    更新日期:2008-12-15 00:00:00

  • Effect of 3,6-anhydro-l-galactose on α-melanocyte stimulating hormone-induced melanogenesis in human melanocytes and a skin-equivalent model.

    abstract::3,6-Anhydro-l-galactose (l-AHG) is a bioactive sugar that is a major component of agarose. Recently, l-AHG was reported to have anti-melanogenic potential in human epidermal melanocytes (HEMs) and B16F10 melanoma cells; however, its underlying molecular mechanisms remain unknown. At noncytotoxic concentrations, l-AHG ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27112

    authors: Kim JH,Kim DH,Cho KM,Kim KH,Kang NJ

    更新日期:2018-09-01 00:00:00

  • Hypoxia-inducible factor 1alpha modulates adhesion, migration, and FAK phosphorylation in vascular smooth muscle cells.

    abstract::Hypoxia promotes angiogenesis by modulating the transcriptional regulator hypoxia-inducible factor 1alpha (HIF-1alpha). HIF-1alpha is a master regulator of the hypoxic response, and its proangiogenic activities include, but are not limited to, regulation of vascular endothelial growth factor (VEGF). The remodeling of ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20559

    authors: Corley KM,Taylor CJ,Lilly B

    更新日期:2005-12-01 00:00:00

  • Modulation of uptake of organic cationic drugs in cultured human colon adenocarcinoma Caco-2 cells by an ecto-alkaline phosphatase activity.

    abstract::Alkaline phosphatase (ALP) refers to a group of nonspecific phosphomonoesterases located primarily in cell plasma membrane. It has been described in different cell lines that ecto-ALP is directly or indirectly involved in the modulation of organic cation transport. We aimed to investigate, in Caco-2 cells, a putative ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10306

    authors: Calhau C,Martel F,Hipólito-Reis C,Azevedo I

    更新日期:2002-01-01 00:00:00

  • Generation of Directly Converted Human Osteoblasts That Are Free of Exogenous Gene and Xenogenic Protein.

    abstract::Generation of osteoblasts from human somatic cells may be applicable in an effective transplantation therapy against bone diseases. Recently we established a procedure to directly convert human fibroblasts into osteoblasts by transducing some transcription factor genes via retroviral vectors. However, retroviral vecto...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25546

    authors: Yamamoto K,Sato Y,Honjo K,Ichioka H,Oseko F,Sowa Y,Yamamoto T,Kanamura N,Kishida T,Mazda O

    更新日期:2016-11-01 00:00:00

  • Characterization of the osteoblast-like cell phenotype under microgravity conditions in the NASA-approved Rotating Wall Vessel bioreactor (RWV).

    abstract::Weightlessness induces bone loss in humans and animal models. We employed the NASA-approved Rotating Wall Vessel bioreactor (RWV) to develop osteoblast-like cell cultures under microgravity and evaluate osteoblast phenotype and cell function. Rat osteoblast-like cell line (ROS.SMER#14) was grown in the RWV at a calcul...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Rucci N,Migliaccio S,Zani BM,Taranta A,Teti A

    更新日期:2002-01-01 00:00:00

  • E-, P-, and N-cadherin are co-expressed in the nasopharyngeal carcinoma cell line TW-039.

    abstract::The cadherin/catenin complex plays a key role in the initiation of cell-cell recognition, and adhesion, and the elaboration of structural and functional organization in multicellular tissues and organs. It is associated with tumor metastasis and also acts as an "invasion suppressor" of cancer cells. Nasopharyngeal car...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:

    authors: Lou PJ,Chen WP,Lin CT,DePhilip RM,Wu JC

    更新日期:1999-11-01 00:00:00

  • Constitutive and inducible expression of cytochromes P4501A (CYP1A1 and CYP1A2) in normal prostate and prostate cancer cells.

    abstract::Constitutive and benzo[a]pyrene (B[a]P) inducible expression of CYP1A1 and CYP1A2 in prostate cancer and normal prostate epithelial cells were examined by immunoblotting. Androgen independent prostate cancer cell lines DU145 and PC3 have constitutive expression of CYP1A and CYP1A1 and CYP1A2, respectively. Four microm...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10753

    authors: Sterling KM Jr,Cutroneo KR

    更新日期:2004-02-01 00:00:00

  • RNA sequencing reveals the circular RNA expression profiles of osteoarthritic synovium.

    abstract::The role of circular RNAs (circRNAs) in regulating cartilage homeostasis in osteoarthritis (OA) has been reported. However, the regulatory mechanisms of circRNAs in OA synovium remains basically unidentified. The current study intended to divulge the expression profile of circRNAs in OA synovium and investigate the po...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29106

    authors: Xiang S,Li Z,Bian Y,Weng X

    更新日期:2019-10-01 00:00:00

  • Downregulation of ARID4A and ARID4B promote tumor progression and directly regulated by microRNA-30d in patient with prostate cancer.

    abstract::AT-rich interaction domain 4A (ARID4A) and AT-rich interaction domain 4B (ARID4B), which are both the AT-rich interaction domain (ARID) family, have been reported to be oncogene or tumor suppressor gene in various human malignances, but there is no involvement about their functions in prostate cancer (PCa). Our previo...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.26913

    authors: Liang YK,Han ZD,Lu JM,Liu ZZ,Zhuo YJ,Zhu XJ,Chen JX,Ye JH,Liang YX,He HC,Zhong WD

    更新日期:2018-09-01 00:00:00

  • Basic fibroblast growth factor decreases type V/XI collagen expression in cultured bovine aortic smooth muscle cells.

    abstract::Vascular smooth muscle cells (SMCs), the major cellular constituent of an artery, synthesize the bulk of fibrillar collagens, including type V/XI, which regulates heterotypic collagen fibril assembly. Basic fibroblast growth factor (bFGF) is a heparin-binding polypeptide growth factor that has been implicated in impor...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4644(19980201)68:2<247::aid-jcb

    authors: Kypreos KE,Sonenshein GE

    更新日期:1998-02-01 00:00:00

  • Retinoblastoma protein complexes with C/EBP proteins and activates C/EBP-mediated transcription.

    abstract::The retinoblastoma protein (RB) recruits histone deacetylase (HDAC) to repress E2F-mediated transactivation that plays a critical role in cell cycle regulation. RB is also involved in activation of expression of a number of tissue specific- and differentiation-related genes. In this study, we examined the mechanism by...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.1239

    authors: Charles A,Tang X,Crouch E,Brody JS,Xiao ZX

    更新日期:2001-08-21 00:00:00

  • TGF-beta2 stimulates cranial suture closure through activation of the Erk-MAPK pathway.

    abstract::Cranial sutures are important growth sites of the skull. During suture closure, the dura mater is one of the most important sources of various positive and negative regulatory signals. Previous results indicate that TGF-beta2 from dura mater strongly accelerates suture closure, however, its exact regulatory mechanism ...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20773

    authors: Lee SW,Choi KY,Cho JY,Jung SH,Song KB,Park EK,Choi JY,Shin HI,Kim SY,Woo KM,Baek JH,Nam SH,Kim YJ,Kim HJ,Ryoo HM

    更新日期:2006-07-01 00:00:00

  • Heterogeneous induction of an invasive phenotype in prostate cancer cells by coculturing with patient-derived fibroblasts.

    abstract::Prostate cancer (PCa) cells frequently invade the surrounding stroma, leading to heterogeneous formation of structural atypia. The surrounding stroma contains multiple functionally diverse populations of fibroblasts that trigger numerous changes in PCa cells including motility. Thus, we hypothesized that direct or ind...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.29893

    authors: Ishii K,Nakagawa Y,Matsuda C,Katoh D,Ichishi M,Shirai T,Hirokawa Y,Fujiwara M,Sugimura Y,Watanabe M

    更新日期:2021-01-22 00:00:00

  • Calmodulin Regulated Spectrin Associated Protein 1 mRNA is Directly Regulated by miR-126 in Primary Human Osteoblasts.

    abstract::Vascularization is essential for bone development, fracture healing, and bone tissue engineering. We have previously described that coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves differentiation of both cell types. Investigating the role of microRNAs (miRNAs)...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25838

    authors: Strassburg S,Nabar N,Lampert F,Goerke SM,Pfeifer D,Finkenzeller G,Stark GB,Simunovic F

    更新日期:2017-07-01 00:00:00

  • Dissecting the roles of individual interactions in protein stability: lessons from a circularized protein.

    abstract::A circular form of bovine pancreatic trypsin inhibitor (BPTI) has been prepared by introducing a peptide bond between the N- and C-termini, which are in close proximity in the native conformation. The pathway and energetics of the disulphide-coupled folding transition of the circular protein have been studied using me...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.240290406

    authors: Goldenberg DP

    更新日期:1985-01-01 00:00:00

  • TGF-beta control of cell proliferation.

    abstract::This article focuses on recent findings that the type V TGF-beta receptor (TbetaR-V), which co-expresses with other TGF-beta receptors (TbetaR-I, TbetaR-II, and TbetaR-III) in all normal cell types studied, is involved in growth inhibition by IGFBP-3 and TGF-beta and that TGF-beta activity is regulated by two distinct...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.20558

    authors: Huang SS,Huang JS

    更新日期:2005-10-15 00:00:00

  • LncRNA MALAT-1 Elevates HMGB1 to Promote Autophagy Resulting in Inhibition of Tumor Cell Apoptosis in Multiple Myeloma.

    abstract::Long non-coding RNAs (lncRNAs) can participate in the pathological process of multiple myeloma (MM) via regulation of specific gene expression and function. This research aimed to study the role of MALAT-1 and the underlying mechanism in MM. In this study, the expression of MALAT-1 and HMGB1 protein in the bone marrow...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25987

    authors: Gao D,Lv AE,Li HP,Han DH,Zhang YP

    更新日期:2017-10-01 00:00:00

  • Decreased activity of acetyl-CoA carboxylase during chemically induced neutrophilic differentiation of human promyelocytic leukemia cells.

    abstract::In order to better understand the mechanism by which changes in the fatty acid composition of cellular lipids occur in leukemia cell lines induced to differentiate, the activity of the first enzyme of fatty acid biosynthesis, acetyl-CoA carboxylase (EC 6.4.1.2) was measured in HL-60 promyelocytic leukemia cells before...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.240260203

    authors: Fischkoff SA,Papuchis GC,Nickols WA

    更新日期:1984-01-01 00:00:00

  • Role of the Helicobacter pylori-induced inflammatory response in the development of gastric cancer.

    abstract::Helicobacter pylori (H. pylori) infection causes chronic gastritis and peptic ulceration and is the strongest risk factor for the development of gastric cancer. The pathogenesis of H. pylori is believed to be associated with infection-initiated chronic gastritis, which is characterized by enhanced expression of many i...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章,评审

    doi:10.1002/jcb.24389

    authors: Lamb A,Chen LF

    更新日期:2013-03-01 00:00:00

  • Membrane receptors for steroid hormones: signal transduction and physiological significance.

    abstract::Membrane receptors for steroid hormones affect signaling pathways that modulate nuclear function, influence neuronal activity, ion flow, and the circulatory system. Indeed, 'new' steroid hormones have been identified by their interaction with membrane-initiated signaling systems. A brief summary of the FASEB Summer Re...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.10409

    authors: Nemere I,Pietras RJ,Blackmore PF

    更新日期:2003-02-15 00:00:00

  • FAP-1-mediated activation of NF-kappaB induces resistance of head and neck cancer to Fas-induced apoptosis.

    abstract::Molecular mechanisms responsible for tumor resistance to apoptosis often involve the Fas/FasL pathway. While squamous cell carcinomas of the head and neck (SCCHN) express both Fas and FasL, their resistance to self-induced apoptosis or apoptosis mediated by Fas agonistic antibody (CH-11Ab) was independent of the level...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20922

    authors: Wieckowski E,Atarashi Y,Stanson J,Sato TA,Whiteside TL

    更新日期:2007-01-01 00:00:00

  • SCN- binding to the charged lysines of histones end domains mimics acetylation and shows the major histone-DNA interactions involved in eu and heterochromatin stabilization.

    abstract::SCN- binds to the charged amino group of lysines, inducing local changes in the electrostatic free energy of histones. We exploited this property to selectively perturb the histone-DNA interactions involved in the stabilization of eu and heterochromatin. Differential scanning calorimetry (DSC) was used as leading tech...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.20689

    authors: Patrone E,Coradeghini R,Barboro P,D'Arrigo C,Mormino M,Parodi S,Balbi C

    更新日期:2006-03-01 00:00:00

  • Identification of pathway-based recurrence-associated signatures in optimally debulked patients with serous ovarian cancer.

    abstract::Serous ovarian cancer (SOC) is the most common form of the histological subtype of epithelial ovarian cancer, with the worst clinical outcome. Despite improvements in surgery and chemotherapy, most patients with SOC experience recurrence within 12-18 months of first-line treatment. Current studies are unable to robust...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27098

    authors: Deng K,Zhang F,Song W,Zhao W,Rong Z,Cai Y,Xu H,Lu M,Wang W,Li A,Hou Y,Li Z,Li K

    更新日期:2018-11-01 00:00:00

  • Real Time Metastatic Route Tracking of Orthotopic PC-3-GFP Human Prostate Cancer Using Intravital Imaging.

    abstract::The cellular basis of metastasis is poorly understood. An important step to understanding this process is to be able to visualize the routes by which cancer cells migrate from the primary tumor to various distant sites to eventually form metastasis. Our laboratory previously developed single-cell in vivo imaging using...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.25391

    authors: Zhang Y,Wang X,Hoffman RM,Seki N

    更新日期:2016-04-01 00:00:00

  • Polycistronic expression of the mitochondrial steroidogenic P450scc system in the HEK293T cell line.

    abstract::The cholesterol hydroxylase/lyase (CHL) system, consisting of cytochrome P450scc, adrenodoxin (Adx) and adrenodoxin reductase (AdR), initiates mammalian steroidogenesis, converting cholesterol to pregnenolone. The foot-and-mouth disease virus 2A-based method allows to express multiple proteins from a single transcript...

    journal_title:Journal of cellular biochemistry

    pub_type: 杂志文章

    doi:10.1002/jcb.27577

    authors: Efimova VS,Isaeva LV,Labudina AA,Tashlitsky VN,Rubtsov MA,Novikova LA

    更新日期:2019-03-01 00:00:00