Abstract：
:This study aimed to evaluate the effect of melatonin on the in vitro culture and maturation of isolated sheep early antral follicles. Isolated early antral follicles were cultured for 12 d in α-minimum essential medium (MEM+) alone (control) or α-MEM+ added with fixed different concentrations (100, 500, or 1,000 pg/mL) or a sequential concentration of melatonin (MelSeq; day 6 = 100; day 12 = 500 pg/mL). The percentage of morphologically normal follicles was higher (P < 0.05) in 500 pg/mL melatonin than the other treatments at 6 d. Mel 500 also showed a higher rate of fully grown oocytes (P < 0.05) than other treatments. After in vitro culture, reactive oxygen species (ROS) levels in oocytes were similar between Mel 500 and MelSeq, with both being lower (P < 0.05) than other treatments. Oocytes cultured in both Mel 500 and Mel 1000 showed glutathione peroxidase levels similar (P > 0.05) to the control group and higher (P < 0.05) than other treatments. Mitochondrial activity was similar (P > 0.05) among control, Mel 500, and Mel 1000 treatments. Mel 500 treatment presented a higher percentage of germinal vesicle breakdown oocytes than the control group and similar percentages to the other treatments. Follicles cultured in melatonin followed by oocyte maturation with the addition of 500 pg/mL melatonin in maturation medium showed increased (P < 0.05) levels of mitochondrial activity compared to α-MEM+ alone. In conclusion, the concentration of 500 pg/mL of melatonin promotes development and decreases ROS levels of ovine oocytes from in vitro grown early antral follicles. Moreover, melatonin increases mitochondrial activity and promotes the acquisition of meiotic competence of these oocytes.
journal_name
Domest Anim Endocrinoljournal_title
Domestic animal endocrinologyauthors
Barros VRP,Monte APO,Santos JMS,Lins TLBG,Cavalcante AYP,Gouveia BB,Müller MC,Oliveira Junior JL,Barberino RS,Donfack NJ,Araújo VR,Matos MHTdoi
10.1016/j.domaniend.2019.106386subject
Has Abstractpub_date
2020-04-01 00:00:00pages
106386eissn
0739-7240issn
1879-0054pii
S0739-7240(19)30064-5journal_volume
71pub_type
杂志文章abstract：:Assisted reproductive technologies, including in vitro embryo production (IVP), have been successfully used in animal reproduction to optimize breeding strategies for improved production and health in animal husbandry. Despite the progress in IVP techniques over the years, further improvements in in vitro embryo cultu...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2015.08.003
更新日期：2016-01-01 00:00:00
abstract：:The effect of endotoxin on follicular growth and on secretion of LH, estradiol-17beta, progesterone and cortisol during the proestrous phase in cattle was investigated. Holstein heifers were treated with PGF2alpha at 11-13 d after ovulation to induce luteolysis. At 42 hr after PGF2alpha treatment, heifers were adminis...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(01)00098-4
更新日期：2001-05-01 00:00:00
abstract：:The flash glucose monitoring system (FGMS) Freestyle Libre provides estimates of blood glucose by constantly measuring the glucose concentration of the interstitial fluid through a sensor inserted in the subcutaneous space. The aim of this study was to evaluate the applicability and accuracy of the FGMS in dogs with d...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2020.106525
更新日期：2021-01-01 00:00:00
abstract：:More than 80% of the milk stored in the udder, i.e. the alveolar fraction, only available after milk ejection is induced by the release of oxytocin and myoepithelial contraction. Milk ejection is induced by tactile teat stimulation, either manually or by the milking machine. The time from the start of a tactile stimul...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2005.02.023
更新日期：2005-08-01 00:00:00
abstract：:The development of a novel enzyme-linked immunosorbent assay (ELISA) for determining luteinizing hormone (LH) in bovine plasma is described. Anti-bovine LH (bLH) monoclonal antibodies (mAbs) were produced and characterized. One mAb recognizing the bLH β subunit was used for immunoaffinity purification of substantial a...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2014.03.004
更新日期：2014-07-01 00:00:00
abstract：:A large amount of research, primarily in mammals, has defined to a great extent the pleiotropic effects of the IGF system on growth, development, and intermediary metabolism. Similar elucidations in poultry were hindered to some extent by the absence of native peptides (IGF-I and IGF-II) until their purification, foll...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0739-7240(97)00019-2
更新日期：1997-07-01 00:00:00
abstract：:The separate and combined effects of Synovex-S (SYN) ear implants and thyroxine (T4)-5'-monodeiodinase inhibition (Trial 1) and T3 injection to create a mild elevation in circulating T3 concentrations (Trial 2) on BW gain and composition of gain were studied. Trial 1 used 24, 285-kg Angus steers in two experimental ph...
journal_title：Domestic animal endocrinology
pub_type： 临床试验,杂志文章
doi：10.1016/0739-7240(92)90030-2
更新日期：1992-07-01 00:00:00
abstract：:Since its discovery in 1994, leptin, a protein hormone synthesized and secreted by adipose tissue, has been shown to regulate feed intake in several species including sheep and pigs. Although a nimiety of information exists regarding the physiological role of leptin in rodents and humans, the regulation and action of ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0739-7240(01)00120-5
更新日期：2001-11-01 00:00:00
abstract：:The present study examined, at identical daily nutrient intakes, the impact of separating protein and lactose intakes across two daily meals on the metabolic and endocrine status in heavy veal calves. Calves were assigned to one of six degrees of separating protein and lactose over the two meals (termed nutrient synch...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2007.01.003
更新日期：2008-02-01 00:00:00
abstract：:The influence of genetic selection for improved feed conversion (FC) and fast growth (GL) in broiler chickens, on the hepatic growth hormone receptor was investigated as a function of age. Selection for improved feed conversion resulted in lean chickens, whereas selection for fast growth resulted in fat chickens. Grow...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(93)90024-6
更新日期：1993-07-01 00:00:00
abstract：:Leptin, mainly produced in adipose tissue (AT), is a protein involved in the central and/or peripheral regulation of body homeostasis, energy intake, storage and expenditure, fertility and immune functions. Its role is well documented in rodent and human species, but less in ruminants. This review is focused on some i...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2005.02.026
更新日期：2005-07-01 00:00:00
abstract：:Bile acids (BAs) are synthesized in the liver via the oxidation of cholesterol and further metabolized by microbiota in the gut, where they simultaneously impact gut function. In the present study, 10 goats were randomly divided into 2 groups; 1 group was injected with dexamethasone (Dex; 0.2 mg/kg), and the other gro...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2018.05.005
更新日期：2018-10-01 00:00:00
abstract：:Hypocalcemia is a common finding in critically ill equine patients. Parathyroid hormone (PTH) helps to maintain calcium homeostasis in hypocalcemic patients by promoting renal calcium reabsorption and bone resorption. Increased serum PTH concentrations have been reported in critically ill people and animals, including...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2020.106470
更新日期：2020-07-01 00:00:00
abstract：:Environment enrichment is a rising topic for animal welfare but measures to identify effective enrichment interventions are lacking. In humans and rodent species, environmental enrichment increases brain-derived neurotrophic factor (BDNF), the most abundant neurotrophin in the brain. Higher BDNF concentration is ultim...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2018.05.007
更新日期：2018-10-01 00:00:00
abstract：:Relatively little is known about the physiological roles of microRNAs (miRNAs) during follicular development. Previous evidence from in vitro studies suggests specific roles for a subset of miRNAs, including miR-21, miR-23a, miR-145, miR-503, miR-224, miR-383, miR-378, miR-132, and miR-212, in regulating ovarian folli...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.06.006
更新日期：2013-10-01 00:00:00
abstract：:The site within the hypothalamic-pituitary axis at which cortisol acts to inhibit luteinizing hormone (LH) secretion was investigated in female pigs. Six ovariectomized, hypophysial stalk-transected (HST) gilts were given 1 microgram pulses of gonadotropin releasing-hormone (GnRH) iv every 45 min from day 0 to 12. On ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(91)90008-8
更新日期：1991-07-01 00:00:00
abstract：:A selection project produced control cows from continuous matings with breed average bulls for predicted transmitting ability for milk (PTA-milk) in 1964 and select cows from matings to four of the highest PTA-milk bulls each year since 1964. Blood samples were collected in 1992 when milk yield difference of select an...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(96)00050-1
更新日期：1996-07-01 00:00:00
abstract：:Crossbred ovariectomized ewes were treated with steroid therapies determined previously to be adequate (progesterone-primed) or inadequate (unprimed) for embryonic development in order to determine actual serum concentrations of replaced steroid hormones achieved by such treatments and to identify secreted endometrial...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(93)90017-6
更新日期：1993-04-01 00:00:00
abstract：:The embryo of the domestic fowl (Gallus domesticus) tenders one distinctive advantage over general mammalian models for investigating the development of the hypothalamo-pituitary-adrenocortical (HPA) axis. This is the relative simplicity with which the embryonic endocrine environment can be influenced without confound...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2004.01.001
更新日期：2004-05-01 00:00:00
abstract：:Dogs do not appear to progress from obesity-induced insulin resistance to type 2 diabetes mellitus. Both postprandial hyperglycemia and postprandial hypertriglyceridemia have been proposed to cause or maintain beta cell failure and progression to type 2 diabetes mellitus in other species. Postprandial glucose, triglyc...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2011.10.002
更新日期：2012-02-01 00:00:00
abstract：:The objectives of this study were to develop an assay for the direct measure of porcine corticosteroid-binding globulin (pCBG) and to confirm age-related changes in plasma pCBG concentration. Isolation and purification of pCBG from plasma was performed by affinity chromatography and HPLC-DEAE anion exchange techniques...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(03)00011-0
更新日期：2003-05-01 00:00:00
abstract：:The effects of weaning and changing post-weaning diet composition on growth patterns and growth-related hormonal profiles were evaluated in neonatal pigs. Forty-eight crossbred piglets were assigned to two groups (n = 24/group) based on weaning at 2 or 3 wk of age (2W and 3W groups. respectively). At weaning, piglets ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(98)00006-x
更新日期：1998-05-01 00:00:00
abstract：:The relationship between in vitro somatotroph function and growth was examined in piglets demonstrating a continuous range of growth characteristics. Twenty barrows were sacrificed at 3 weeks of age for the collection of pituitary tissue and blood. Pituitary cells from each animal were cultured and exposed to vehicle ...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(94)90008-6
更新日期：1994-10-01 00:00:00
abstract：:Intact (n = 17) and unilaterally hysterectomized-ovariectomized (UHOX, n = 19) gilts were used to study conceptus development and the steroidogenic capability of three regions of the porcine placenta under normal and crowded intrauterine conditions. Gilts were hysterectomized on either Days 30, 50, 70, or 90 of gestat...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/0739-7240(94)00009-p
更新日期：1995-01-01 00:00:00
abstract：:Remarkable diversity of size and health of offspring exists after normal pregnancies. When pregnancies are complicated by an extrinsic variable such as inappropriate maternal nutrition, birth weight and health of the neonate are substantially affected. The placenta is the organ through which respiratory gases, nutrien...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.domaniend.2004.06.006
更新日期：2004-10-01 00:00:00
abstract：:Adipose tissue (AT) depots are heterogeneous in terms of morphology and adipocyte metabolism. Adiponectin, one of the most abundant adipokines, is known for its insulin sensitizing effects and its role in glucose and lipid metabolism. Little is known about the presence of adiponectin protein in visceral (vc) and subcu...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2013.12.001
更新日期：2014-04-01 00:00:00
abstract：:Three experiments were conducted to better understand why milking-induced prolactin (PRL) release decreases as lactation advances. Experiment 1 compared the effects of milking, 2-min manual stimulation of the mammary gland (without milking), or injection of 1 IU of oxytocin (without milking) on hormonal release in ear...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2018.10.003
更新日期：2019-01-01 00:00:00
abstract：:Adrenocorticotropic hormone (ACTH) challenges are frequently performed in repetition when evaluating stress or welfare in animals. To our knowledge, the repeatability of ACTH challenges in cattle, although fundamental to further studies of this type, has not yet been the subject of research. Therefore, the objective o...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2019.03.001
更新日期：2020-07-01 00:00:00
abstract：:beta-Casein (CSN2) is a major milk protein in most mammals. The CSN2 gene is generally induced by lactogenic hormones bound to its promoter. The expression of this gene can be enhanced by signal transducers and activators of transcription (STAT) and glucocorticoid receptor (GR). Here, we analyzed the promoter and intr...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/j.domaniend.2008.04.001
更新日期：2008-10-01 00:00:00
abstract：:Age-dependent interactions between environmental temperature and porcine growth hormone (pGH) treatment on the function of the somatotrophic axis were evaluated in the neonatal pig. At 3 d of age, 40 Landrace x Yorkshire x Duroc piglets received intraperitoneal implants containing either recombinant pGH (0.5 mg/d; n =...
journal_title：Domestic animal endocrinology
pub_type： 杂志文章
doi：10.1016/s0739-7240(99)00003-x
更新日期：1999-02-01 00:00:00
