A universal adhesive incorporating antimicrobial peptide nisin: effects on Streptococcus mutans and saliva-derived multispecies biofilms.

Abstract:

:For purpose of enhancing the antibacterial activity of a universal adhesive, the antimicrobial peptide nisin was incorporated into Single Bond Universal and its antibacterial effect on Streptococcus mutans monospecific biofilms and saliva-derived multispecies biofilms was studied. Nisin was incorporated into Single Bond Universal and the antibacterial activity was examined by confocal laser scanning microscopy (CLSM), reverse transcription-quantitative polymerase chain reaction (qRT-PCR), phenol-sulfuric acid method and lactate dehydrogenase enzymatic method. The bonding properties were tested by microtensile bond strength (μTBS) and degree of conversion (DC). Data were analyzed by one-way analysis of variance (ANOVA) and least significant difference multiple comparison tests (P < 0.05). The Single Bond Universal incorporated with 3% (w/v) nisin could significantly inhibit the growth of the S. mutans monospecific biofilms (P< 0.01) and decrease the expression of genes related to extracellular polysaccharide (EPS) synthesis (gtfB, gtfC, gtfD and spaP) and acidogenicity (ldh) (P < 0.05). 3% (w/v) nisin-incorporated Single Bond Universal could also inhibit the growth of saliva-derived multispecies biofilms and decrease the excretion of EPS and lactic acid ( P< 0.05). μTBS and DC of 3% (w/v) nisin-incorporated Single Bond Universal did not deteriorate obviously (P > 0.05). In conclusion, 3% (w/v) nisin-incorporated Single Bond Universal substantially inhibited the growth of both S. mutans monospecific and saliva-derived multispecies biofilms without compromising the bonding properties.

journal_name

Odontology

journal_title

Odontology

authors

Zhao M,Qu Y,Liu J,Mai S,Gu L

doi

10.1007/s10266-019-00478-8

subject

Has Abstract

pub_date

2020-07-01 00:00:00

pages

376-385

issue

3

eissn

1618-1247

issn

1618-1255

pii

10.1007/s10266-019-00478-8

journal_volume

108

pub_type

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