Abstract：
:Selenoprotein F (Selenof) is an endoplasmic reticulum (ER)-resident protein. It may be functionally linked to glycoprotein folding in the ER but the detail function is not fully understood. To study the function of Selenof, we used CRISPR/Cas9 to generate Selenof knockout mice and performed proteomic analysis of hepatic proteins by iTRAQ. Collectively, 83 differently expressed proteins (DEPs) were identified in the liver of Selenof knockout mice. The changes of mRNA and protein levels of 6 selected DEPs, Fatty acid synthase, ATP-citrate synthase, Glutathione S-transferase P 1, Transformer-2 protein homolog beta, Pyruvate kinase, Metallothionein-2, were further verified by quantitative real-time PCR or Western blot. The roles of 83 DEPs are mainly related to metabolism and cancer. Consistently, the levels of NADPH and ATP, two molecules closely related to energy metabolism, are significantly changed in the livers of Selenof knockout mice. SIGNIFICANCE: Our study identified potential biological pathways and proteins related to Selenof deletion. These findings will provide possible proteins/pathways related to Selenof and help to understand the function of Selenof as well as the relationship between Selenof and certain diseases.
journal_name
J Proteomicsjournal_title
Journal of proteomicsauthors
Zheng X,Ren B,Wang H,Huang R,Zhou J,Liu H,Tian J,Huang Kdoi
10.1016/j.jprot.2020.103653subject
Has Abstractpub_date
2020-03-20 00:00:00pages
103653eissn
1874-3919issn
1876-7737pii
S1874-3919(20)30021-Xjournal_volume
215pub_type
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