Rapid exchange of synaptic and extrasynaptic NMDA receptors in hippocampal CA1 neurons.

Abstract:

:N-methyl-d-aspartate receptors (NMDARs) are fundamental coincidence detectors of synaptic activity necessary for the induction of synaptic plasticity and synapse stability. Adjusting NMDAR synaptic content, whether by receptor insertion or lateral diffusion between extrasynaptic and synaptic compartments, could play a substantial role defining the characteristics of the NMDAR-mediated excitatory postsynaptic current (EPSC), which in turn would mediate the ability of the synapse to undergo plasticity. Lateral NMDAR movement has been observed in dissociated neurons; however, it is currently unclear whether NMDARs are capable of lateral surface diffusion in hippocampal slices, a more physiologically relevant environment. To test for lateral mobility in rat hippocampal slices, we rapidly blocked synaptic NMDARs using MK-801, a use-dependent and irreversible NMDAR blocker. Following a 5-min washout period, we observed a strong recovery of NMDAR-mediated responses. The degree of the observed recovery was proportional to the amount of induced blockade, independent of levels of intracellular calcium, and mediated primarily by GluN2B-containing NMDA receptors. These results indicate that lateral diffusion of NMDARs could be a mechanism by which synapses rapidly adjust parameters to fine-tune synaptic plasticity.NEW & NOTEWORTHYN-methyl-d-aspartate-type glutamate receptors (NMDARs) have always been considered stable components of synapses. We show that in rat hippocampal slices synaptic NMDARs are in constant exchange with extrasynaptic receptors. This exchange of receptors is mediated primarily by NMDA receptors containing GluN2B, a subunit necessary to undergo synaptic plasticity. Thus this lateral movement of synaptic receptors allows synapses to rapidly regulate the total number of synaptic NMDARs with potential consequences for synaptic plasticity.

journal_name

J Neurophysiol

authors

McQuate A,Barria A

doi

10.1152/jn.00458.2019

subject

Has Abstract

pub_date

2020-03-01 00:00:00

pages

1004-1014

issue

3

eissn

0022-3077

issn

1522-1598

journal_volume

123

pub_type

杂志文章
  • Responses of rat lateral hypothalamic neuron activity to dorsal raphe nuclei stimulation.

    abstract::1. The effects of dorsal raphe (DR) stimulation on neural activity in the rat lateral hypothalamic area (LHA), including specific glucose-sensitive neurons, were investigated by extracellular and intracellular recording in vivo, and the neurotransmitters involved were determined. 2. In 67 adult male anesthetized rats,...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1988.60.2.524

    authors: Kai Y,Oomura Y,Shimizu N

    更新日期:1988-08-01 00:00:00

  • Voltage-dependent, pertussis toxin insensitive inhibition of calcium currents by histamine in bovine adrenal chromaffin cells.

    abstract::Histamine is a known secretagogue in adrenal chromaffin cells. Activation of G-protein linked H(1) receptors stimulates phospholipase C, which generates inositol trisphosphate leading to release of intracellular calcium stores and stimulation of calcium influx through store operated and other channels. This calcium le...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.83.3.1435

    authors: Currie KP,Fox AP

    更新日期:2000-03-01 00:00:00

  • LTD and LTP at the developing retinogeniculate synapse.

    abstract::The purpose of the present study was to determine whether retinal activity can support long-term changes in synaptic strength in the developing dorsal lateral geniculate nucleus (LGN) of thalamus. To test for this we made use of a rodent in vitro explant preparation in which retinal afferents and the intrinsic circuit...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.90618.2008

    authors: Ziburkus J,Dilger EK,Lo FS,Guido W

    更新日期:2009-12-01 00:00:00

  • Conductance-based model of the voltage-dependent generation of a plateau potential in subthalamic neurons.

    abstract::Because the subthalamic nucleus (STN) acts as a driving force of the basal ganglia, it is important to know how the activities of STN neurons are regulated. Previously, we have reported that a subset of STN neurons generates a plateau potential in a voltage-dependent manner. These plateau potentials can be evoked only...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00508.2003

    authors: Otsuka T,Abe T,Tsukagawa T,Song WJ

    更新日期:2004-07-01 00:00:00

  • Response patterns along an isofrequency contour in cat primary auditory cortex (AI) to stimuli varying in average and interaural levels.

    abstract::The topographical response of a portion of an isofrequency contour in primary cat auditory cortex (AI) to a series of monaural and binaural stimuli was studied. Responses of single neurons to monaural and a matrix of binaural characteristic frequency tones, varying in average binaural level (ABL) and interaural level ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00171.2003

    authors: Nakamoto KT,Zhang J,Kitzes LM

    更新日期:2004-01-01 00:00:00

  • Hemispheric differences in the relationship between corticomotor excitability changes following a fine-motor task and motor learning.

    abstract::Motor performance induces a postexercise increase in corticomotor excitability that may be associated with motor learning. We investigated whether there are hemispheric differences in the extent and/or time course of changes in corticomotor excitability following a manipulation task (Purdue pegboard) and their relatio...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00595.2003

    authors: Garry MI,Kamen G,Nordstrom MA

    更新日期:2004-04-01 00:00:00

  • Acute effect of an incision on mechanosensitive afferents in the plantar rat hindpaw.

    abstract::The purpose of this study was to examine which primary afferent fibers are sensitized to mechanical stimuli after an experimental surgical incision to the glabrous skin of the rat hindpaw. Afferent fibers teased from the L(5) dorsal root or the tibial nerve were recorded in anesthetized rats. The mechanical response p...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00207.2001

    authors: Hämäläinen MM,Gebhart GF,Brennan TJ

    更新日期:2002-02-01 00:00:00

  • Selective effects of light exposure on distribution of motility in the chick embryo at E18.

    abstract::It is well established that orderly patterns of motor neuron activity, muscle recruitment, and limb movement are generated in chicks during motility by embryonic day (E)9, the midpoint in embryonic development. However, our recent work suggests that some attributes of motility, such as the rhythm of repetitive limb mo...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00393.2003

    authors: Bradley NS,Jahng DY

    更新日期:2003-09-01 00:00:00

  • Calibration of visually guided reaching is driven by error-corrective learning and internal dynamics.

    abstract::The sensorimotor calibration of visually guided reaching changes on a trial-to-trial basis in response to random shifts in the visual feedback of the hand. We show that a simple linear dynamical system is sufficient to model the dynamics of this adaptive process. In this model, an internal variable represents the curr...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00897.2006

    authors: Cheng S,Sabes PN

    更新日期:2007-04-01 00:00:00

  • The magnitude of the somatosensory cortical activity is related to the mobility and strength impairments seen in children with cerebral palsy.

    abstract::The noted disruption of thalamocortical connections and abnormalities in tactile sensory function has resulted in a new definition of cerebral palsy (CP) that recognizes the sensorimotor integration process as central to the motor impairments seen in these children. Despite this updated definition, the connection betw...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00602.2014

    authors: Kurz MJ,Heinrichs-Graham E,Becker KM,Wilson TW

    更新日期:2015-05-01 00:00:00

  • Electrical feedback in the cone pedicle: a computational analysis.

    abstract::One of the fundamental principles of neuroscience is that direct electrical interactions between neurons are not possible without specialized electrical contacts, gap junctions, because the transmembrane resistance of neurons is typically much higher than the resistance of the adjacent extracellular space. However it ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00098.2005

    authors: Dmitriev AV,Mangel SC

    更新日期:2006-03-01 00:00:00

  • Postnatal development of the motor representation in primary motor cortex.

    abstract::The purpose of this study was to examine when the muscles and joints of the forelimb become represented in primary motor cortex (M1) during postnatal life and how local representation patterns change. We examined these questions in cats that were anesthetized (45-90 days, n = 14; adults, n = 3) and awake (n = 4; 52-86...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.84.5.2582

    authors: Chakrabarty S,Martin JH

    更新日期:2000-11-01 00:00:00

  • Both visual and idiothetic cues contribute to head direction cell stability during navigation along complex routes.

    abstract::Successful navigation requires a constantly updated neural representation of directional heading, which is conveyed by head direction (HD) cells. The HD signal is predominantly controlled by visual landmarks, but when familiar landmarks are unavailable, self-motion cues are able to control the HD signal via path integ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.01041.2010

    authors: Yoder RM,Clark BJ,Brown JE,Lamia MV,Valerio S,Shinder ME,Taube JS

    更新日期:2011-06-01 00:00:00

  • Nonprimary auditory thalamic representation of acoustic change.

    abstract::1. The mismatch response, or mismatch negativity (MMN), is a neurophysiologic response to stimulus change. In humans and other animals, the MMN may underlie the ability to discriminate acoustic differences, a fundamental aspect of auditory perception. 2. This study investigated the role of the thalamus in the generati...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1994.72.3.1270

    authors: Kraus N,McGee T,Littman T,Nicol T,King C

    更新日期:1994-09-01 00:00:00

  • Covert orienting of attention in macaques. I. Effects of behavioral context.

    abstract::1. A task was used by Posner (1980) to measure shifts of attention that occurred covertly, in the absence of an eye movement or other orienting response. This paradigm was used here to assess the nature of covert attentional orienting in monkeys to develop an animal model for neurophysiological studies. Shifts of atte...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1993.70.1.431

    authors: Bowman EM,Brown VJ,Kertzman C,Schwarz U,Robinson DL

    更新日期:1993-07-01 00:00:00

  • Activity of commissural interneurons in spinal cord of Xenopus embryos.

    abstract::Horseradish peroxidase- (HRP) filled microelectrodes have been used to examine the anatomy and physiology of "commissural interneurons," a morphologically defined class of spinal cord interneuron in Xenopus laevis embryos. Commissural interneurons have unipolar cell bodies in the dorsal half of the spinal cord. Their ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1984.51.6.1257

    authors: Soffe SR,Clarke JD,Roberts A

    更新日期:1984-06-01 00:00:00

  • Neuronal activity in macaque supplementary eye field during planning of saccades in response to pattern and spatial cues.

    abstract::The aim of this study was to determine whether neuronal activity in the macaque supplementary eye field (SEF) is influenced by the rule used for saccadic target selection. Two monkeys were trained to perform a variant of the memory-guided saccade task in which any of four visible dots (rightward, upward, leftward, and...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2000.84.3.1369

    authors: Olson CR,Gettner SN,Ventura V,Carta R,Kass RE

    更新日期:2000-09-01 00:00:00

  • Organization of suppression in receptive fields of neurons in cat visual cortex.

    abstract::1. The response to an optimally oriented stimulus of both simple and complex cells in the cat's striate visual cortex (area 17) can be suppressed by the superposition of an orthogonally oriented drifting grating. This effect is referred to as cross-orientation suppression. We have examined the spatial organization and...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1992.68.1.144

    authors: DeAngelis GC,Robson JG,Ohzawa I,Freeman RD

    更新日期:1992-07-01 00:00:00

  • Effects of visual cortical lesions on receptive-field properties of single units in superior colliculus of the rabbit.

    abstract::1. One hundred seventy-nine single units were studied in the superior colliculus of seven Dutch-belted rabbits following ablation of the ipsilateral visual cortex. The response characteristics of these units were compared with those of 284 single units recorded from the superior colliculus of 20 intact animals. 2. In ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1982.47.2.272

    authors: Graham J,Berman N,Murphy EH

    更新日期:1982-02-01 00:00:00

  • Human manual control precision depends on vestibular sensory precision and gravitational magnitude.

    abstract::Precise motion control is critical to human survival on Earth and in space. Motion sensation is inherently imprecise, and the functional implications of this imprecision are not well understood. We studied a "vestibular" manual control task in which subjects attempted to keep themselves upright with a rotational hand ...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00565.2018

    authors: Rosenberg MJ,Galvan-Garza RC,Clark TK,Sherwood DP,Young LR,Karmali F

    更新日期:2018-12-01 00:00:00

  • Prefrontal cortical representation of visuospatial working memory in monkeys examined by local inactivation with muscimol.

    abstract::In primates, dorsolateral areas of the prefrontal cortex (PFC) play a major role in visuospatial working memory. To examine the functional organization of the PFC for representing visuospatial working memory, we produced reversible local inactivation, with the local injection of muscimol (5 microg, 1 microl), at vario...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.2001.86.4.2041

    authors: Sawaguchi T,Iba M

    更新日期:2001-10-01 00:00:00

  • A novel protocol to investigate motor training-induced plasticity and sensorimotor integration in the cerebellum and motor cortex.

    abstract::Our group set out to develop a sensitive technique, capable of detecting output changes from the posterior fossa following a motor acquisition task. Transcranial magnetic stimulation (TMS) was applied over the right cerebellar cortex 5 ms in advance of test stimuli over the left cerebral motor cortex (M1), suppressing...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00661.2013

    authors: Baarbé J,Yielder P,Daligadu J,Behbahani H,Haavik H,Murphy B

    更新日期:2014-02-01 00:00:00

  • Functional properties of neurons in the primate tongue primary motor cortex during swallowing.

    abstract::Recent studies conducted in our laboratory have suggested that the tongue primary motor cortex (i.e., tongue-MI) plays a critical role in the control of voluntary tongue movements in the primate. However, the possible involvement of tongue-MI in semiautomatic tongue movements, such as those in swallowing, remains unkn...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.78.3.1516

    authors: Martin RE,Murray GM,Kemppainen P,Masuda Y,Sessle BJ

    更新日期:1997-09-01 00:00:00

  • Deficits of visual attention and saccadic eye movements after lesions of parietooccipital cortex in monkeys.

    abstract::1. Visual attention is often profoundly disturbed in humans after damage to the cortex of the posterior parietal lobe, particularly of the minor hemisphere, with some patients being totally unaware of visual stimuli in the hemifield of extrapersonal space contralateral to the cortical damage. This severe form of visua...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1989.61.1.74

    authors: Lynch JC,McLaren JW

    更新日期:1989-01-01 00:00:00

  • Contribution of voltage-gated Ca2+ channels to homosynaptic long-term depression in the CA1 region in vitro.

    abstract::Homosynaptic long-term depression (LTD) of synaptic efficacy was induced in field excitatory postsynaptic potentials by administration of 900 pulses at either 1 or 3 Hz in 2- to 3-wk-old Sprague-Dawley rats. The stimulation was administered via a bipolar stimulating electrode placed immediately adjacent to the recordi...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.1997.77.3.1651

    authors: Christie BR,Schexnayder LK,Johnston D

    更新日期:1997-03-01 00:00:00

  • Neural correlates of attention and distractibility in the lateral intraparietal area.

    abstract::We examined the activity of neurons in the lateral intraparietal area (LIP) during a task in which we measured attention in the monkey, using an advantage in contrast sensitivity as our definition of attention. The animals planned a memory-guided saccade but made or canceled it depending on the orientation of a briefl...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00848.2005

    authors: Bisley JW,Goldberg ME

    更新日期:2006-03-01 00:00:00

  • Approaching threatening stimuli cause an expansion of defensive peripersonal space.

    abstract::When sudden environmental stimuli signaling threat occur in the portion of space surrounding the body (defensive peripersonal space), defensive responses are enhanced. Recently Bisio et al. (Bisio A, Garbarini F, Biggio M, Fossataro C, Ruggeri P, Bove M. J Neurosci 37: 2415-2424, 2017) showed that a marker of defensiv...

    journal_title:Journal of neurophysiology

    pub_type: 评论,杂志文章

    doi:10.1152/jn.00316.2017

    authors: Bufacchi RJ

    更新日期:2017-10-01 00:00:00

  • Relationships between neck muscle electromyography and three-dimensional head kinematics during centrally induced torsional head perturbations.

    abstract::The relationship between neck muscle electromyography (EMG) and torsional head rotation (about the nasooccipital axis) is difficult to assess during normal gaze behaviors with the head upright. Here, we induced acute head tilts similar to cervical dystonia (torticollis) in two monkeys by electrically stimulating 20 in...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00312.2012

    authors: Farshadmanesh F,Byrne P,Wang H,Corneil BD,Crawford JD

    更新日期:2012-12-01 00:00:00

  • Effect of stochastic synaptic and dendritic dynamics on synaptic plasticity in visual cortex and hippocampus.

    abstract::Various forms of synaptic plasticity, including spike timing-dependent plasticity, can be accounted for by calcium-dependent models of synaptic plasticity. However, recent results in which synaptic plasticity is induced by multi-spike protocols cannot simply be accounted for by linear superposition of plasticity due t...

    journal_title:Journal of neurophysiology

    pub_type: 杂志文章

    doi:10.1152/jn.00895.2006

    authors: Cai Y,Gavornik JP,Cooper LN,Yeung LC,Shouval HZ

    更新日期:2007-01-01 00:00:00

  • Neuromuscular adaptation during skill acquisition on a two degree-of-freedom target-acquisition task: isometric torque production.

    abstract::In this study we attempted to identify the principles that govern the changes in neural control that occur during repeated performance of a multiarticular coordination task. Eight participants produced isometric flexion/extension and pronation/supination torques at the radiohumeral joint, either in isolation (e.g., fl...

    journal_title:Journal of neurophysiology

    pub_type: 临床试验,杂志文章

    doi:10.1152/jn.00670.2004

    authors: Shemmell J,Forner M,Tresilian JR,Riek S,Barry BK,Carson RG

    更新日期:2005-11-01 00:00:00