Abstract：
:Rabies virus (RABV), the etiological agent for the lethal disease of rabies, is a deadly zoonotic pathogen. The RABV glycoprotein (RABV-G) is a key factor mediating virus entry and the major target of neutralizing antibodies. Here, we report the crystal structures of RABV-G solved in the free form at ∼pH-8.0 and in the complex form with a neutralizing antibody 523-11 at ∼pH-6.5, respectively. RABV-G has three domains, and the basic-to-acidic pH change results in large domain re-orientations and concomitant domain-linker re-constructions, switching it from a bent hairpin conformation into an extended conformation. During such low-pH-induced structural transitions, residues located in the domain-linker are found to play important roles in glycoprotein-mediated membrane fusion. Finally, the antibody interacts with RABV-G mainly through its heavy chain and binds to a bipartite conformational epitope in the viral protein for neutralization. These structures provide valuable information for vaccine and drug design.
journal_name
Cell Host Microbejournal_title
Cell host & microbeauthors
Yang F,Lin S,Ye F,Yang J,Qi J,Chen Z,Lin X,Wang J,Yue D,Cheng Y,Chen Z,Chen H,You Y,Zhang Z,Yang Y,Yang M,Sun H,Li Y,Cao Y,Yang S,Wei Y,Gao GF,Lu Gdoi
10.1016/j.chom.2019.12.012subject
Has Abstractpub_date
2020-03-11 00:00:00pages
441-453.e7issue
3eissn
1931-3128issn
1934-6069pii
S1931-3128(19)30641-9journal_volume
27pub_type
杂志文章abstract：:Crosstalk between immune cells and the microbiota in mucosal tissues can set an individual on a trajectory toward health or disease. Little is known about these early-life events in the human respiratory tract. We examined bacterial colonization and immune system maturation in the lower airways over the first year of ...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2018.10.019
更新日期：2018-12-12 00:00:00
abstract：:Influenza A virus (IAV) infection is initiated by the attachment of the viral glycoprotein hemagglutinin (HA) to sialic acid on the host cell surface. However, the sialic acid-containing receptor crucial for IAV infection has remained unidentified. Here, we show that HA binds to the voltage-dependent Ca2+ channel Cav1...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2018.04.015
更新日期：2018-06-13 00:00:00
abstract：:Complement, a complex system of serum proteins, contributes to host defense through multiple mechanisms. In this issue of Cell Host & Microbe, Sorbara et al. (2018) discovered that cytosolic pathogens opsonized by complement are restricted by autophagy. This unexpected role for complement is important for cell-autonom...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2018.04.010
更新日期：2018-05-09 00:00:00
abstract：:Hepatitis C virus (HCV) is a major global health burden accounting for around 170 million chronic infections worldwide. Although highly potent direct-acting antiviral drugs to treat chronic hepatitis C have been approved recently, owing to their high costs and limited availability and a large number of undiagnosed inf...
journal_title：Cell host & microbe
pub_type： 杂志文章,评审
doi：10.1016/j.chom.2014.10.008
更新日期：2014-11-12 00:00:00
abstract：:The human microbiome encodes extensive metabolic capabilities, but our understanding of the mechanisms linking gut microbes to human metabolism remains limited. Here, we focus on the conversion of cholesterol to the poorly absorbed sterol coprostanol by the gut microbiota to develop a framework for the identification ...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2020.05.013
更新日期：2020-08-12 00:00:00
abstract：:Productive transcription of the HIV-1 genome involves RNA polymerase II working in concert with the viral protein Tat and its team of cofactors in a highly orchestrated process. Now, Pagans and colleagues report that the lysine methyltransferase Set7/9-KMT7 associates with Tat to stimulate RNA polymerase II elongation...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2010.03.001
更新日期：2010-03-18 00:00:00
abstract：:Cell migration and invasion require the coordinated regulation of cytoskeletal architectural changes by signaling factors, including the actin-binding protein cortactin. Bacterial and viral pathogens subvert these signaling factors to promote their uptake, spread and dissemination. We show that the gastric pathogen He...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2011.05.007
更新日期：2011-06-16 00:00:00
abstract：:iNKT cells are innate T lymphocytes recognizing endogenous and foreign lipid antigens presented in the MHC-like molecule CD1d. The semi-invariant iNKT cell TCR can detect certain bacterial and parasitic lipids and drive iNKT cell responses. How iNKT cells respond to fungi, however, is unknown. We found that CD1d-defic...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2011.09.011
更新日期：2011-11-17 00:00:00
abstract：:Parasites undergo complex life cycles that comprise a wide variety of cellular differentiation events in different host compartments and transmission across multiple hosts. As parasites depend on host resources, it is not surprising they have developed efficient mechanisms to sense alterations and adapt to the availab...
journal_title：Cell host & microbe
pub_type： 杂志文章,评审
doi：10.1016/j.chom.2018.05.018
更新日期：2018-06-13 00:00:00
abstract：:In this issue of Cell Host & Microbe, Walton et al. (2018) uncover the mycobacterial factors that activate VEGF signaling and promote aberrant angiogenesis in the tuberculous granuloma. Preventing abnormal angiogenesis in the granuloma represents a potential therapeutic approach for tuberculosis. ...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2018.09.016
更新日期：2018-10-10 00:00:00
abstract：:How gut cells distinguish between beneficial symbionts and deleterious pathobionts is a central question. In this issue of Cell Host & Microbe, Kim et al. (2020) demonstrate that the nucleoside catabolism pathway controlling bacterial uracil and ribose production is an essential trigger of the commensal to pathogen tr...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2020.02.008
更新日期：2020-03-11 00:00:00
abstract：:The ecological dynamics underlying the coexistence between antagonistic populations of bacteria and their viruses, bacteriophages (phages), in the mammalian gut microbiota remain poorly understood. We challenged a murine synthetic bacterial community with phages to study the factors allowing phages-bacteria coexistenc...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2020.06.002
更新日期：2020-09-09 00:00:00
abstract：:Animal-microbe facultative symbioses play a fundamental role in ecosystem and organismal health. Yet, due to the flexible nature of their association, the selection pressures that act on animals and their facultative symbionts remain elusive. Here we apply experimental evolution to Drosophila melanogaster associated w...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2018.06.001
更新日期：2018-07-11 00:00:00
abstract：:In a recent Science paper, Teles et al. (2013) show that type I and II interferons (IFNs) are reciprocally expressed in the polar immune forms of leprosy, with type I IFNs inducing IL-10 that interferes with the antimycobacterial effects of type II IFNs (IFNγ) at the site of infection. ...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2013.04.001
更新日期：2013-04-17 00:00:00
abstract：:In response to virus infection, cells can alter protein expression to modify cellular functions and limit viral replication. To examine host protein expression during infection with human cytomegalovirus (HCMV), an enveloped DNA virus, we performed a semiquantitative, temporal analysis of the cell surface proteome in ...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2012.05.012
更新日期：2012-07-19 00:00:00
abstract：:Leishmaniasis is a chronic infection in which intracellular parasites avoid destruction by the immune system. Using intravital imaging, Filipe-Santos et al. (2009) demonstrate that some parasitized dendritic cells receive much less attention than others during their choreographed dance with T cells, suggesting that th...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章,评审
doi：10.1016/j.chom.2009.07.002
更新日期：2009-07-23 00:00:00
abstract：:Here I revisit our early experiments published in Cell Host & Microbe (Turnbaugh et al., 2008) showing that a diet rich in fat and simple sugars alters the gut microbiome in a manner that contributes to host adiposity, and reflect upon the remarkable advances and remaining challenges in this field. ...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2017.02.021
更新日期：2017-03-08 00:00:00
abstract：:The switch from culture-based enumeration to deep sequencing has enabled microbial community composition to be profiled en masse. In a new article, Maurice et al. (2013) report the use of fluorescence-activated cell sorting (FACS) to perform a high-throughput analysis of gut microbiota community function. ...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2013.01.011
更新日期：2013-02-13 00:00:00
abstract：:Lujo virus (LUJV) is an arenavirus that emerged in 2008 associated with a cluster of human cases of severe hemorrhagic fever. In this issue of Cell Host & Microbe, Raaben et al. (2017) identify neuropilin (NRP)-2 as cell surface receptor and the tetraspannin protein CD63 as intracellular entry factor for LUJV. ...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2017.10.014
更新日期：2017-11-08 00:00:00
abstract：:Vaccines are the most effective means available for preventing infectious diseases. However, vaccine-induced immune responses are highly variable between individuals and between populations in different regions of the world. Understanding the basis of this variation is, thus, of fundamental importance to human health....
journal_title：Cell host & microbe
pub_type： 杂志文章,评审
doi：10.1016/j.chom.2020.06.014
更新日期：2020-08-12 00:00:00
abstract：:Much of the mutualistic relationship between humans and their resident intestinal Bacteroides species is founded on glycans. The host provides plant polysaccharides and host-derived glycans and, in return, receives beneficial end products of bacterial fermentation. Glycans from the bacteria themselves are required for...
journal_title：Cell host & microbe
pub_type： 杂志文章,评审
doi：10.1016/j.chom.2009.05.010
更新日期：2009-06-18 00:00:00
abstract：:Systemic fungal infections trigger marked immune-regulatory disturbances, but the mechanisms are poorly understood. We report that the pathogenic yeast of Blastomyces dermatitidis elaborates dipeptidyl-peptidase IVA (DppIVA), a close mimic of the mammalian ectopeptidase CD26, which modulates critical aspects of hemato...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2016.02.001
更新日期：2016-03-09 00:00:00
abstract：:The role of gut bacteria in complications of heart inflammation has previously not been fully understood. Gil-Cruz et al. (2019) demonstrate a commensal Bacteroides species that triggers a cross-immune response against a bacterial protein and heart epitope, causing cardiomyopathy. This study links commensal bacteria w...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2019.12.011
更新日期：2020-01-08 00:00:00
abstract：:Human type A Enteroviruses (EV-As) cause diseases ranging from hand-foot-and-mouth disease to poliomyelitis-like disease. Although cellular receptors are identified for some EV-As, they remain elusive for the majority of EV-As. We identify the cell surface molecule KREMEN1 as an entry receptor for coxsackievirus A10 (...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2018.03.019
更新日期：2018-05-09 00:00:00
abstract：:Bacterial CRISPR-Cas systems employ RNA-guided nucleases to destroy phage (viral) DNA. Phages, in turn, have evolved diverse "anti-CRISPR" proteins (Acrs) to counteract acquired immunity. In Listeria monocytogenes, prophages encode two to three distinct anti-Cas9 proteins, with acrIIA1 always present. However, the sig...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2020.04.001
更新日期：2020-07-08 00:00:00
abstract：:Antigenic diversity has posed a critical barrier to vaccine development against the pathogenic blood-stage infection of the human malaria parasite Plasmodium falciparum. To date, only strain-specific protection has been reported by trials of such vaccines in nonhuman primates. We recently showed that P. falciparum ret...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2014.11.017
更新日期：2015-01-14 00:00:00
abstract：:The inner colon mucus layer capacity to separate bacteria from the epithelium is dependent on bacterial colonizers signaling to the host epithelium. In this issue of Cell Host & Microbe, Wlodarska et al. (2017) demonstrate that the mucin-utilizing Peptostreptococcus russellii protects the host from inflammatory diseas...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2017.06.011
更新日期：2017-07-12 00:00:00
abstract：:Type I interferons (IFNs) are considered to be the universal mechanism by which viral infections are controlled. However, many IFN-stimulated genes (ISGs) rely on antiviral pathways that are toxic to host cells, which may be detrimental in nonrenewable cell types, such as neurons. We show that dorsal root ganglionic (...
journal_title：Cell host & microbe
pub_type： 杂志文章
doi：10.1016/j.chom.2012.07.013
更新日期：2012-09-13 00:00:00
abstract：:Although anthrax toxin was identified as a major Bacillus anthracis virulence factor over 50 years ago, defining the physiologically relevant targets has been challenging. Liu et al. demonstrate that intoxication of myeloid-derived cells contributes to establishing infection but is not required for mortality resulting...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2010.10.011
更新日期：2010-11-18 00:00:00
abstract：:Regulatory T cells (Tregs) are required to establish immune tolerance to commensal microbes. Tregs accumulate abruptly in the skin during a defined window of postnatal tissue development. However, the mechanisms mediating Treg migration to neonatal skin are unknown. Here we show that hair follicle (HF) development fac...
journal_title：Cell host & microbe
pub_type： 评论,杂志文章
doi：10.1016/j.chom.2017.03.001
更新日期：2017-04-12 00:00:00
