Evolving Populations in Biofilms Contain More Persistent Plasmids.

Abstract:

:Bacterial plasmids substantially contribute to the rapid spread of antibiotic resistance, which is a crisis in healthcare today. Coevolution of plasmids and their hosts promotes this spread of resistance by ameliorating the cost of plasmid carriage. However, our knowledge of plasmid-bacteria coevolution is solely based on studies done in well-mixed liquid cultures, even though biofilms represent the main way of bacterial life on Earth and are responsible for most infections. The spatial structure and the heterogeneity provided by biofilms are known to lead to increased genetic diversity as compared with well-mixed liquids. Therefore, we expect that growth in this complex environment could affect the evolutionary trajectories of plasmid-host dyads. We experimentally evolved Shewanella oneidensis MR-1 with plasmid pBP136Gm in biofilms and chemostats and sequenced the genomes of clones and populations. Biofilm populations not only maintained a higher diversity of mutations than chemostat populations but contained a few clones with markedly more persistent plasmids that evolved via multiple distinct trajectories. These included the acquisition of a putative toxin-antitoxin transposon by the plasmid and chromosomal mutations. Some of these genetic changes resulted in loss of plasmid transferability or decrease in plasmid cost. Growth in chemostats led to a higher proportion of variants with decreased plasmid persistence, a phenomenon not detected in biofilms. We suggest that the presence of more stable plasmid-host dyads in biofilms reflects higher genetic diversity and possibly unknown selection pressures. Overall, this study underscores the importance of the mode of growth in the evolution of antibiotic-resistant bacteria.

journal_name

Mol Biol Evol

authors

Stalder T,Cornwell B,Lacroix J,Kohler B,Dixon S,Yano H,Kerr B,Forney LJ,Top EM

doi

10.1093/molbev/msaa024

subject

Has Abstract

pub_date

2020-06-01 00:00:00

pages

1563-1576

issue

6

eissn

0737-4038

issn

1537-1719

pii

5728644

journal_volume

37

pub_type

杂志文章
  • Evolution of the Zfx and Zfy genes: rates and interdependence between the genes.

    abstract::A phylogenetic analysis of sex-chromosomal zinc-finger genes (Zfx and Zfy) indicates that the genes have not evolved completely independently since their initial separation. The sequence similarities suggest gene conversion in the last exon between the duplicated Y-chromosomal genes Zfy-1 and Zfy-2 in the mouse. There...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a040003

    authors: Pamilo P,Bianchi NO

    更新日期:1993-03-01 00:00:00

  • Distortions in genealogies due to purifying selection.

    abstract::Purifying selection can substantially alter patterns of molecular evolution. Its main effect is to reduce overall levels of genetic variation, leading to a reduced effective population size. However, it also distorts genealogies relative to neutral expectations. A structured coalescent method has been used to describe...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/mss170

    authors: Nicolaisen LE,Desai MM

    更新日期:2012-11-01 00:00:00

  • Local adaptation and vector-mediated population structure in Plasmodium vivax malaria.

    abstract::Plasmodium vivax in southern Mexico exhibits different infectivities to 2 local mosquito vectors, Anopheles pseudopunctipennis and Anopheles albimanus. Previous work has tied these differences in mosquito infectivity to variation in the central repeat motif of the malaria parasite's circumsporozoite (csp) gene, but su...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msn073

    authors: Joy DA,Gonzalez-Ceron L,Carlton JM,Gueye A,Fay M,McCutchan TF,Su XZ

    更新日期:2008-06-01 00:00:00

  • Divergent evolution and evolution by the birth-and-death process in the immunoglobulin VH gene family.

    abstract::Immunoglobulin diversity is generated primarily by the heavy- and light-chain variable-region gene families. To understand the pattern of long-term evolution of the heavy-chain variable-region (VH) gene family, which is composed of a large number of member genes, the evolutionary relationships of representative VH gen...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a040127

    authors: Ota T,Nei M

    更新日期:1994-05-01 00:00:00

  • Heterotypy in the N-terminal region of growth/differentiation factor 5 (GDF5) mature protein during teleost evolution.

    abstract::Heterotypy is now recognized as a generative force in the formation of new proteins through modification of existing proteins. We report that heterotypy in the N-terminal region of the mature growth/differentiation factor 5 (GDF5) protein occurred during evolution of teleosts. N-terminal length variation of GDF5 was f...

    journal_title:Molecular biology and evolution

    pub_type: 信件,评审

    doi:10.1093/molbev/msn041

    authors: Fujimura K,Terai Y,Ishiguro N,Miya M,Nishida M,Okada N

    更新日期:2008-05-01 00:00:00

  • A genome scan to detect candidate regions influenced by local natural selection in human populations.

    abstract::As human populations dispersed throughout the world, they were subjected to new selective forces, which must have led to local adaptation via natural selection and hence altered patterns of genetic variation. Yet, there are very few examples known in which such local selection has clearly influenced human genetic vari...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msg092

    authors: Kayser M,Brauer S,Stoneking M

    更新日期:2003-06-01 00:00:00

  • Meiotic recombination dramatically decreased in thelytokous queens of the little fire ant and their sexually produced workers.

    abstract::The little fire ant, Wasmannia auropunctata, displays a peculiar breeding system polymorphism. Classical haplo-diploid sexual reproduction between reproductive individuals occurs in some populations, whereas, in others, queens and males reproduce clonally. Workers are produced sexually and are sterile in both clonal a...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msr082

    authors: Rey O,Loiseau A,Facon B,Foucaud J,Orivel J,Cornuet JM,Robert S,Dobigny G,Delabie JH,Mariano Cdos S,Estoup A

    更新日期:2011-09-01 00:00:00

  • Geographic variation and positive selection on M7 lysin, an acrosomal sperm protein in mussels (Mytilus spp.).

    abstract::Successful fertilization in free-spawning marine organisms depends on the interactions between genes expressed on the surfaces of eggs and sperm. Positive selection frequently characterizes the molecular evolution of such genes, raising the possibility that some common deterministic process drives the evolution of gam...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msl062

    authors: Riginos C,Wang D,Abrams AJ

    更新日期:2006-10-01 00:00:00

  • DNA-DNA hybridization evidence of the rapid rate of muroid rodent DNA evolution.

    abstract::Single-copy nuclear DNAs (scnDNAs) of eight species of arvicoline and six species of murine rodents were compared using DNA-DNA hybridization. The branching pattern derived from the DNA comparisons is congruent with the fossil evidence and supported by comparative biochemical, chromosomal, and morphological studies. T...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a040444

    authors: Catzeflis FM,Sheldon FH,Ahlquist JE,Sibley CG

    更新日期:1987-05-01 00:00:00

  • Evolution of Sp transcription factors.

    abstract::The Sp family of transcription factors binds GC-rich DNA sequences. The ubiquitously expressed Sp1 and Sp3 have been well characterized in mammals. Presented here is the characterization of the only Sp protein expressed in the liver or heart tissue of the teleost fish Fundulus heteroclitus. This protein, fSp3, is most...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a004074

    authors: Kolell KJ,Crawford DL

    更新日期:2002-03-01 00:00:00

  • Non-African populations of Drosophila melanogaster have a unique origin.

    abstract::Drosophila melanogaster is widely used as a model in DNA variation studies. Patterns of polymorphism have, however, been affected by the history of this species, which is thought to have recently spread out of Africa to the rest of the world. We analyzed DNA sequence variation in 11 populations, including four contine...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msh089

    authors: Baudry E,Viginier B,Veuille M

    更新日期:2004-08-01 00:00:00

  • Gene turnover in the avian globin gene families and evolutionary changes in hemoglobin isoform expression.

    abstract::The apparent stasis in the evolution of avian chromosomes suggests that birds may have experienced relatively low rates of gene gain and loss in multigene families. To investigate this possibility and to explore the phenotypic consequences of variation in gene copy number, we examined evolutionary changes in the famil...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msu341

    authors: Opazo JC,Hoffmann FG,Natarajan C,Witt CC,Berenbrink M,Storz JF

    更新日期:2015-04-01 00:00:00

  • Mitochondrial Mutation Rate, Spectrum and Heteroplasmy in Caenorhabditis elegans Spontaneous Mutation Accumulation Lines of Differing Population Size.

    abstract::Mitochondrial genomes of metazoans, given their elevated rates of evolution, have served as pivotal markers for phylogeographic studies and recent phylogenetic events. In order to determine the dynamics of spontaneous mitochondrial mutations in small populations in the absence and presence of selection, we evolved mut...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msx051

    authors: Konrad A,Thompson O,Waterston RH,Moerman DG,Keightley PD,Bergthorsson U,Katju V

    更新日期:2017-06-01 00:00:00

  • High proportions of deleterious polymorphisms in constrained human genes.

    abstract::Previous studies on human mitochondrial genomes showed that the ratio of intra-specific diversities at nonsynonymous-to-synonymous positions was two to ten times higher than the ratio of interspecific divergences at these positions, suggesting an excess of slightly deleterious nonsynonymous polymorphisms. However, suc...

    journal_title:Molecular biology and evolution

    pub_type: 信件

    doi:10.1093/molbev/msq287

    authors: Subramanian S

    更新日期:2011-01-01 00:00:00

  • Intra- and interspecific variation of the CCR5 gene in higher primates.

    abstract::We have evaluated the molecular evolution of the chemokine receptor CCR5 in primates. The chemokine receptor CCR5 serves as a major co-receptor for human immunodeficiency virus/simian immunodeficiency virus (HIV/SIV) infection. Knowledge of evolution of the CCR5 molecule and selection on the CCR5 gene may shed light o...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msg198

    authors: Zhang YW,Ryder OA,Zhang YP

    更新日期:2003-10-01 00:00:00

  • Restriction-map variation in the alcohol dehydrogenase region of Drosophila pseudoobscura.

    abstract::A 32-kb region including the Adh structural gene was analyzed with six restriction endonucleases in 20 lines of Drosophila pseudoobscura, one line of D. persimilis, and one line of D. miranda. Nineteen lines of D. pseudoobscura from a single population were estimated to be polymorphic at one in every 15 nucleotides (p...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a040438

    authors: Schaeffer SW,Aquadro CF,Anderson WW

    更新日期:1987-05-01 00:00:00

  • Denisovan Ancestry in East Eurasian and Native American Populations.

    abstract::Although initial studies suggested that Denisovan ancestry was found only in modern human populations from island Southeast Asia and Oceania, more recent studies have suggested that Denisovan ancestry may be more widespread. However, the geographic extent of Denisovan ancestry has not been determined, and moreover the...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msv141

    authors: Qin P,Stoneking M

    更新日期:2015-10-01 00:00:00

  • Estimates of DNA and protein sequence divergence: an examination of some assumptions.

    abstract::Some of the assumptions underlying estimates of DNA and protein sequence divergence are examined. A solution for the variance of these estimates that allows for different mutation rates and different population sizes in each species and for an arbitrary structure in the initial population is obtained. It is shown that...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a040303

    authors: Golding GB

    更新日期:1983-12-01 00:00:00

  • Expansion of hexose transporter genes was associated with the evolution of aerobic fermentation in yeasts.

    abstract::The genetic basis of organisms' adaptation to different environments is a central issue of molecular evolution. The budding yeast Saccharomyces cerevisiae and its relatives predominantly ferment glucose into ethanol even in the presence of oxygen. This was suggested to be an adaptation to glucose-rich habitats, but th...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msq184

    authors: Lin Z,Li WH

    更新日期:2011-01-01 00:00:00

  • Proteomics and comparative genomic investigations reveal heterogeneity in evolutionary rate of male reproductive proteins in mice (Mus domesticus).

    abstract::Male reproductive fitness is strongly affected by seminal fluid. In addition to interacting with the female environment, seminal fluid mediates important physiological characteristics of sperm, including capacitation and motility. In mammals, the male reproductive tract shows a striking degree of compartmentalization,...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msp094

    authors: Dean MD,Clark NL,Findlay GD,Karn RC,Yi X,Swanson WJ,MacCoss MJ,Nachman MW

    更新日期:2009-08-01 00:00:00

  • Evolution of the AID/APOBEC family of polynucleotide (deoxy)cytidine deaminases.

    abstract::The AID/APOBEC family (comprising AID, APOBEC1, APOBEC2, and APOBEC3 subgroups) contains members that can deaminate cytidine in RNA and/or DNA and exhibit diverse physiological functions (AID and APOBEC3 deaminating DNA to trigger pathways in adaptive and innate immunity; APOBEC1 mediating apolipoprotein B RNA editing...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msi026

    authors: Conticello SG,Thomas CJ,Petersen-Mahrt SK,Neuberger MS

    更新日期:2005-02-01 00:00:00

  • Environmental sequence data from the Sargasso Sea reveal that the characteristics of genome reduction in Prochlorococcus are not a harbinger for an escalation in genetic drift.

    abstract::The marine cyanobacterium Prochlorococcus MED4 has the smallest sequenced genome of any photosynthetic organism. Prochlorococcus MED4 shares many genomic characteristics with chloroplasts and bacterial endosymbionts, including a reduced coding capacity, missing DNA repair genes, a minimal transcriptional regulatory ne...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msn217

    authors: Hu J,Blanchard JL

    更新日期:2009-01-01 00:00:00

  • Predominant gain of promoter TATA box after gene duplication associated with stress responses.

    abstract::TATA box, the core promoter element, exists in a broad range of eukaryotes, and the expression of TATA-containing genes usually responds to various environmental stresses. Hence, the evolution of TATA-box in duplicate genes may provide some clues for the interrelationship among environmental stress, expression differe...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msr116

    authors: Zou Y,Huang W,Gu Z,Gu X

    更新日期:2011-10-01 00:00:00

  • The shoot stem cell niche in angiosperms: expression patterns of WUS orthologues in rice and maize imply major modifications in the course of mono- and dicot evolution.

    abstract::In Arabidopsis, stem cell homeostasis in the shoot apical meristem (SAM) is controlled by a feedback loop between WUS and CLV functions. We have identified WUS orthologues in maize and rice by a detailed phylogenetic analysis of the WOX gene family and subsequent cloning. A single WUS orthologue is present in the rice...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msl125

    authors: Nardmann J,Werr W

    更新日期:2006-12-01 00:00:00

  • Ecological nitrogen limitation shapes the DNA composition of plant genomes.

    abstract::Phenotypes and behaviors respond to resource constraints via adaptation, but the influence of ecological limitations on the composition of eukaryotic genomes is still unclear. We trace connections between plant ecology and genomes through their elemental composition. Inorganic sources of nitrogen (N) are severely limi...

    journal_title:Molecular biology and evolution

    pub_type: 信件

    doi:10.1093/molbev/msp038

    authors: Acquisti C,Elser JJ,Kumar S

    更新日期:2009-05-01 00:00:00

  • The emerging limbs and twigs of the East Asian mtDNA tree.

    abstract::We determine the phylogenetic backbone of the East Asian mtDNA tree by using published complete mtDNA sequences and assessing both coding and control region variation in 69 Han individuals from southern China. This approach assists in the interpretation of published mtDNA data on East Asians based on either control re...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a003996

    authors: Kivisild T,Tolk HV,Parik J,Wang Y,Papiha SS,Bandelt HJ,Villems R

    更新日期:2002-10-01 00:00:00

  • Horizontal transfer and selection in the evolution of P elements.

    abstract::The roles of selection and horizontal transfer in the evolution of the canonical subfamily of P: elements were studied in the saltans and willistoni species groups of the genus Drosophila (subgenus Sophophora). We estimate that the common ancestor of the canonical P: subfamily dates back 2-3 Myr at the most, despite t...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a026253

    authors: Silva JC,Kidwell MG

    更新日期:2000-10-01 00:00:00

  • Population Parameters Underlying an Ongoing Soft Sweep in Southeast Asian Malaria Parasites.

    abstract::Multiple kelch13 alleles conferring artemisinin resistance (ART-R) are currently spreading through Southeast Asian malaria parasite populations, providing a unique opportunity to observe an ongoing soft selective sweep, investigate why resistance alleles have evolved multiple times and determine fundamental population...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msw228

    authors: Anderson TJ,Nair S,McDew-White M,Cheeseman IH,Nkhoma S,Bilgic F,McGready R,Ashley E,Pyae Phyo A,White NJ,Nosten F

    更新日期:2017-01-01 00:00:00

  • Male-driven evolution of mitochondrial and chloroplastidial DNA sequences in plants.

    abstract::Although there is substantial evidence that, in animals, male-inherited neutral DNA evolves at a higher rate than female-inherited DNA, the relative evolutionary rate of male- versus female-inherited DNA has not been investigated in plants. We compared the substitution rates at neutral sites of maternally and paternal...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/oxfordjournals.molbev.a004151

    authors: Whittle CA,Johnston MO

    更新日期:2002-06-01 00:00:00

  • The Footprint of Polygenic Adaptation on Stress-Responsive Cis-Regulatory Divergence in the Arabidopsis Genus.

    abstract::Adaptation of a complex trait often requires the accumulation of many modifications to finely tune its underpinning molecular components to novel environmental requirements. The investigation of cis-acting regulatory modifications can be used to pinpoint molecular systems partaking in such complex adaptations. Here, w...

    journal_title:Molecular biology and evolution

    pub_type: 杂志文章

    doi:10.1093/molbev/msw096

    authors: He F,Arce AL,Schmitz G,Koornneef M,Novikova P,Beyer A,de Meaux J

    更新日期:2016-08-01 00:00:00