Morphologically Different Pectobacterium brasiliense Bacteriophages PP99 and PP101: Deacetylation of O-Polysaccharide by the Tail Spike Protein of Phage PP99 Accompanies the Infection.

Abstract:

:Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infection mechanism for more rational composition of therapeutic cocktails. We present the study of two bacteriophages, infective for the Pectobacterium brasiliense strain F152. Podoviridae PP99 is a representative of the genus Zindervirus, and Myoviridae PP101 belongs to the still unclassified genomic group. The structure of O-polysaccharide of F152 was established by sugar analysis and 1D and 2D NMR spectroscopy: → 4)-α-D-Manp6Ac-(1→ 2)-α-D-Manp-(1→ 3)-β-D-Galp-(1→ 3 ↑ 1 α -l- 6 dTal p Ac 0 - 2 The recombinant tail spike protein of phage PP99, gp55, was shown to deacetylate the side chain talose residue of bacterial O-polysaccharide, thus providing the selective attachment of the phage to the cell surface. Both phages demonstrate lytic behavior, thus being prospective for therapeutic purposes.

journal_name

Front Microbiol

authors

Lukianova AA,Shneider MM,Evseev PV,Shpirt AM,Bugaeva EN,Kabanova AP,Obraztsova EA,Miroshnikov KK,Senchenkova SN,Shashkov AS,Toschakov SV,Knirel YA,Ignatov AN,Miroshnikov KA

doi

10.3389/fmicb.2019.03147

subject

Has Abstract

pub_date

2020-01-23 00:00:00

pages

3147

issn

1664-302X

journal_volume

10

pub_type

杂志文章
  • Staphylococcus aureus Entrance into the Dairy Chain: Tracking S. aureus from Dairy Cow to Cheese.

    abstract::Staphylococcus aureus is one of the most important contagious mastitis pathogens in dairy cattle. Due to its zoonotic potential, control of S. aureus is not only of great economic importance in the dairy industry but also a significant public health concern. The aim of this study was to decipher the potential of bovin...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.01603

    authors: Kümmel J,Stessl B,Gonano M,Walcher G,Bereuter O,Fricker M,Grunert T,Wagner M,Ehling-Schulz M

    更新日期:2016-10-13 00:00:00

  • Corrigendum: Evaluation of Human Milk Microbiota by 16S rRNA Gene Next-Generation Sequencing (NGS) and Cultivation/MALDI-TOF Mass Spectrometry Identification.

    abstract::[This corrects the article DOI: 10.3389/fmicb.2019.02612.]. ...

    journal_title:Frontiers in microbiology

    pub_type: 已发布勘误

    doi:10.3389/fmicb.2020.00031

    authors: Treven P,Mahnič A,Rupnik M,Golob M,Pirš T,Matijašić BB,Lorbeg PM

    更新日期:2020-01-29 00:00:00

  • Genome Comparisons of Candida glabrata Serial Clinical Isolates Reveal Patterns of Genetic Variation in Infecting Clonal Populations.

    abstract::Candida glabrata is an opportunistic fungal pathogen that currently ranks as the second most common cause of candidiasis. Although the mechanisms underlying virulence and drug resistance in C. glabrata are now starting to be elucidated, we still lack a good understanding of how this yeast adapts during the course of a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.00112

    authors: Carreté L,Ksiezopolska E,Gómez-Molero E,Angoulvant A,Bader O,Fairhead C,Gabaldón T

    更新日期:2019-02-12 00:00:00

  • EPS-SJ Exopolisaccharide Produced by the Strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 Is Involved in Adhesion to Epithelial Intestinal Cells and Decrease on E. coli Association to Caco-2 Cells.

    abstract::The aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coli's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the pres...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00286

    authors: Živković M,Miljković MS,Ruas-Madiedo P,Markelić MB,Veljović K,Tolinački M,Soković S,Korać A,Golić N

    更新日期:2016-03-09 00:00:00

  • Genetic Variants of the DSF Quorum Sensing System in Stenotrophomonas maltophilia Influence Virulence and Resistance Phenotypes Among Genotypically Diverse Clinical Isolates.

    abstract::The pathogenicity of Stenotrophomonas maltophilia is regulated in part by its quorum sensing (QS) system. The main QS signaling molecule in S. maltophilia is known as diffusible signal factor (DSF), and the rpf gene cluster is responsible for its synthesis and perception. Two cluster variants have been previously desc...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.01160

    authors: Yero D,Huedo P,Conchillo-Solé O,Martínez-Servat S,Mamat U,Coves X,Llanas F,Roca I,Vila J,Schaible UE,Daura X,Gibert I

    更新日期:2020-06-03 00:00:00

  • Chlamydia Persistence: A Survival Strategy to Evade Antimicrobial Effects in-vitro and in-vivo.

    abstract::The Chlamydiaceae comprise a group of highly adapted bacterial pathogens sharing a unique intracellular lifestyle. Three Chlamydia species are pathogenic to humans: Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci. C. trachomatis is the leading bacterial cause of sexually-transmitted infections and ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2018.03101

    authors: Panzetta ME,Valdivia RH,Saka HA

    更新日期:2018-12-12 00:00:00

  • Nutrient Composition Promotes Switching between Pellicle and Bottom Biofilm in Salmonella.

    abstract::Salmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to antimicrobials and d...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.02160

    authors: Paytubi S,Cansado C,Madrid C,Balsalobre C

    更新日期:2017-11-07 00:00:00

  • Response of Tomato Rhizosphere Bacteria to Root-Knot Nematodes, Fenamiphos and Sampling Time Shows Differential Effects on Low Level Taxa.

    abstract::A factorial taxonomic metabarcoding study was carried out to determine the effect of root-knot nematodes (Meloidogyne incognita, RKN) and the nematocide fenamiphos on the rhizosphere microbiome of tomato. Plants inoculated (or not) with RKN second-stage juveniles (J2), and treated (or not) with the nematocide, were te...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00390

    authors: Colagiero M,Rosso LC,Catalano D,Schena L,Ciancio A

    更新日期:2020-03-20 00:00:00

  • Spatial Distribution of Root and Crown Rot Fungi Associated With Winter Wheat in the North China Plain and Its Relationship With Climate Variables.

    abstract::The distribution frequency of pathogenic fungi associated with root and crown rot of winter wheat (Triticum aestivum) from 104 fields in the North China Plain was determined during the period from 2013 to 2016. The four most important species identified were Bipolaris sorokiniana (24.0% from roots; 33.7% from stems), ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01054

    authors: Xu F,Yang G,Wang J,Song Y,Liu L,Zhao K,Li Y,Han Z

    更新日期:2018-05-25 00:00:00

  • Species interactions differ in their genetic robustness.

    abstract::Conflict and cooperation between bacterial species drive the composition and function of microbial communities. Stability of these emergent properties will be influenced by the degree to which species' interactions are robust to genetic perturbations. We use genome-scale metabolic modeling to computationally analyze t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00271

    authors: Chubiz LM,Granger BR,Segrè D,Harcombe WR

    更新日期:2015-04-14 00:00:00

  • Diatom-Bacteria Interactions Modulate the Composition and Productivity of Benthic Diatom Biofilms.

    abstract::Benthic diatoms are dominant primary producers in intertidal mudflats and constitute a major source of organic carbon to consumers and decomposers residing within these ecosystems. They typically form biofilms whose species richness, community composition and productivity can vary in response to environmental drivers ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01255

    authors: Koedooder C,Stock W,Willems A,Mangelinckx S,De Troch M,Vyverman W,Sabbe K

    更新日期:2019-06-05 00:00:00

  • Food-bacteria interplay: pathometabolism of emetic Bacillus cereus.

    abstract::Bacillus cereus is a Gram-positive endospore forming bacterium known for its wide spectrum of phenotypic traits, enabling it to occupy diverse ecological niches. Although the population structure of B. cereus is highly dynamic and rather panmictic, production of the emetic B. cereus toxin cereulide is restricted to st...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2015.00704

    authors: Ehling-Schulz M,Frenzel E,Gohar M

    更新日期:2015-07-14 00:00:00

  • Microbial community composition of transiently wetted Antarctic Dry Valley soils.

    abstract::During the summer months, wet (hyporheic) soils associated with ephemeral streams and lake edges in the Antarctic Dry Valleys (DVs) become hotspots of biological activity and are hypothesized to be an important source of carbon and nitrogen for arid DV soils. Recent research in the DV has focused on the geochemistry a...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00009

    authors: Niederberger TD,Sohm JA,Gunderson TE,Parker AE,Tirindelli J,Capone DG,Carpenter EJ,Cary SC

    更新日期:2015-01-28 00:00:00

  • Ebolavirus Replication and Tetherin/BST-2.

    abstract::Ebolavirus (EBOV) is an enveloped, non-segmented, negative-stranded RNA virus, which consists of five species: Zaire ebolavirus, Sudan ebolavirus, Tai Forest ebolavirus, Bundibugyo ebolavirus, and Reston ebolavirus. EBOV causes a lethal hemorrhagic fever in both humans and non-human primates. The EBOV RNA genome encod...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00111

    authors: Yasuda J

    更新日期:2012-04-02 00:00:00

  • The Promoting Effect of Gut Microbiota on Growth and Development of Red Palm Weevil, Rhynchophorus ferrugineus (Olivier) (Coleoptera: Dryophthoridae) by Modulating Its Nutritional Metabolism.

    abstract::Red palm weevil (RPW), Rhynchophorus ferrugineus Olivier, is a destructive pest for palm trees worldwide. Recent studies have shown that RPW gut is colonized by microbes and alterations in gut microbiota can significantly modify its hemolymph nutrition content. However, the exact effects of gut microbiota on RPW pheno...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01212

    authors: Habineza P,Muhammad A,Ji T,Xiao R,Yin X,Hou Y,Shi Z

    更新日期:2019-05-29 00:00:00

  • The Claudin Family Protein FigA Mediates Ca2+ Homeostasis in Response to Extracellular Stimuli in Aspergillus nidulans and Aspergillus fumigatus.

    abstract::The claudin family protein Fig1 is a unique fungal protein that is involved in pheromone-induced calcium influx and membrane fusion during the mating of Saccharomyces cerevisiae and Candida albicans. Whether and how Fig1 regulates Ca2+ homeostasis in response to extracellular stimuli is poorly understood. Previously, ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00977

    authors: Qian H,Chen Q,Zhang S,Lu L

    更新日期:2018-05-15 00:00:00

  • Phosphoproteomics analysis of a clinical Mycobacterium tuberculosis Beijing isolate: expanding the mycobacterial phosphoproteome catalog.

    abstract::Reversible protein phosphorylation, regulated by protein kinases and phosphatases, mediates a switch between protein activity and cellular pathways that contribute to a large number of cellular processes. The Mycobacterium tuberculosis genome encodes 11 Serine/Threonine kinases (STPKs) which show close homology to euk...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00006

    authors: Fortuin S,Tomazella GG,Nagaraj N,Sampson SL,Gey van Pittius NC,Soares NC,Wiker HG,de Souza GA,Warren RM

    更新日期:2015-02-10 00:00:00

  • Sterilization of liquid foods by pulsed electric fields-an innovative ultra-high temperature process.

    abstract::The intention of this study was to investigate the inactivation of endospores by a combined thermal and pulsed electric field (PEF) treatment. Therefore, self-cultivated spores of Bacillus subtilis and commercial Geobacillus stearothermophilus spores with certified heat resistance were utilized. Spores of both strains...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2015.00400

    authors: Reineke K,Schottroff F,Meneses N,Knorr D

    更新日期:2015-05-06 00:00:00

  • The Role of Strigolactone in the Cross-Talk Between Arabidopsis thaliana and the Endophytic Fungus Mucor sp.

    abstract::Over the last years the role of fungal endophytes in plant biology has been extensively studied. A number of species were shown to positively affect plant growth and fitness, thus attempts have been made to utilize these microorganisms in agriculture and phytoremediation. Plant-fungi symbiosis requires multiple metabo...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.00441

    authors: Rozpądek P,Domka AM,Nosek M,Ważny R,Jędrzejczyk RJ,Wiciarz M,Turnau K

    更新日期:2018-03-19 00:00:00

  • Bacillus cereus cell response upon exposure to acid environment: toward the identification of potential biomarkers.

    abstract::Microorganisms are able to adapt to different environments and evolve rapidly, allowing them to cope with their new environments. Such adaptive response and associated protections toward other lethal stresses, is a crucial survival strategy for a wide spectrum of microorganisms, including food spoilage bacteria, patho...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章,评审

    doi:10.3389/fmicb.2013.00284

    authors: Desriac N,Broussolle V,Postollec F,Mathot AG,Sohier D,Coroller L,Leguerinel I

    更新日期:2013-10-02 00:00:00

  • Vitamin b(1) and b(12) uptake and cycling by plankton communities in coastal ecosystems.

    abstract::While vitamin B(12) has recently been shown to co-limit the growth of coastal phytoplankton assemblages, the cycling of B-vitamins in coastal ecosystems is poorly understood as planktonic uptake rates of vitamins B(1) and B(12) have never been quantified in tandem in any aquatic ecosystem. The goal of this study was t...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2012.00363

    authors: Koch F,Hattenrath-Lehmann TK,Goleski JA,Sañudo-Wilhelmy S,Fisher NS,Gobler CJ

    更新日期:2012-10-12 00:00:00

  • Screening of Bifidobacteria and Lactobacilli Able to Antagonize the Cytotoxic Effect of Clostridium difficile upon Intestinal Epithelial HT29 Monolayer.

    abstract::Clostridium difficile is an opportunistic pathogen inhabiting the human gut, often being the aetiological agent of infections after a microbiota dysbiosis following, for example, an antibiotic treatment. C. difficile infections (CDI) constitute a growing health problem with increasing rates of morbidity and mortality ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00577

    authors: Valdés-Varela L,Alonso-Guervos M,García-Suárez O,Gueimonde M,Ruas-Madiedo P

    更新日期:2016-04-22 00:00:00

  • Deficiency in Lipoteichoic Acid Synthesis Causes a Failure in Executing the Colony Developmental Program in Bacillus subtilis.

    abstract::Colonies are an abundant form of bacterial multicellularity; however, relatively little is known about the initial stages of their construction. We have previously described that colony development of the soil bacterium Bacillus subtilis is a highly ordered process, typically initiating with the formation of extending...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01991

    authors: Mamou G,Fiyaksel O,Sinai L,Ben-Yehuda S

    更新日期:2017-10-24 00:00:00

  • PipY, a Member of the Conserved COG0325 Family of PLP-Binding Proteins, Expands the Cyanobacterial Nitrogen Regulatory Network.

    abstract::Synechococcus elongatus PCC 7942 is a paradigmatic model organism for nitrogen regulation in cyanobacteria. Expression of genes involved in nitrogen assimilation is positively regulated by the 2-oxoglutarate receptor and global transcriptional regulator NtcA. Maximal activation requires the subsequent binding of the c...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01244

    authors: Labella JI,Cantos R,Espinosa J,Forcada-Nadal A,Rubio V,Contreras A

    更新日期:2017-07-11 00:00:00

  • The Anti-mycobacterial Activity of a Diterpenoid-Like Molecule Operates Through Nitrogen and Amino Acid Starvation.

    abstract::A library of 14 minimally cytotoxic diterpenoid-like compounds (CC50 > 70 μM on HepG2 human liver cells) was screened against Mycobacterium smegmatis, Staphylococcus aureus, and Escherichia coli to determine antimicrobial activity. Some compounds with a phenethyl alcohol (PE) core substituted with a β-cyclocitral deri...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2019.01444

    authors: Crusco A,Baptista R,Bhowmick S,Beckmann M,Mur LAJ,Westwell AD,Hoffmann KF

    更新日期:2019-06-25 00:00:00

  • Metagenomic Analysis of Bacteria, Fungi, Bacteriophages, and Helminths in the Gut of Giant Pandas.

    abstract::To obtain full details of gut microbiota, including bacteria, fungi, bacteriophages, and helminths, in giant pandas (GPs), we created a comprehensive microbial genome database and used metagenomic sequences to align against the database. We delineated a detailed and different gut microbiota structures of GPs. A total ...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.01717

    authors: Yang S,Gao X,Meng J,Zhang A,Zhou Y,Long M,Li B,Deng W,Jin L,Zhao S,Wu D,He Y,Li C,Liu S,Huang Y,Zhang H,Zou L

    更新日期:2018-07-31 00:00:00

  • Investigation of a Salmonellosis Outbreak Caused by Multidrug Resistant Salmonella Typhimurium in China.

    abstract::The rapid emergence of multidrug resistant Salmonella is a global public-health concern as outbreaks in recent years have mostly been caused by multidrug resistant strains. Here, we evaluated an outbreak in China caused by multidrug resistant Salmonella enterica serovar Typhimurium (S. Typhimurium) by employing an epi...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2020.00801

    authors: Xiang Y,Li F,Dong N,Tian S,Zhang H,Du X,Zhou X,Xu X,Yang H,Xie J,Yang C,Liu H,Qiu S,Song H,Sun Y

    更新日期:2020-04-29 00:00:00

  • A Trypsin Inhibitor from Tecoma stans Leaves Inhibits Growth and Promotes ATP Depletion and Lipid Peroxidation in Candida albicans and Candida krusei.

    abstract::Tecoma stans (yellow elder) has shown medicinal properties and antimicrobial activity. Previous reports on antifungal activity of T. stans preparations and presence of trypsin inhibitor activity from T. stans leaves stimulated the investigation reported here. In this work, we proceeded to the purification and characte...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2016.00611

    authors: Patriota LL,Procópio TF,de Souza MF,de Oliveira AP,Carvalho LV,Pitta MG,Rego MJ,Paiva PM,Pontual EV,Napoleão TH

    更新日期:2016-04-27 00:00:00

  • Potential Risk Areas of Aedes albopictus in South-Eastern Iran: A Vector of Dengue Fever, Zika, and Chikungunya.

    abstract::The possibility of the rapid and global spread of Zika, chikungunya, yellow fever, and dengue fever by Aedes albopictus is well documented and may be facilitated by changes in climate. To avert and manage health risks, climatic and topographic information can be used to model and forecast which areas may be most prone...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2017.01660

    authors: Nejati J,Bueno-Marí R,Collantes F,Hanafi-Bojd AA,Vatandoost H,Charrahy Z,Tabatabaei SM,Yaghoobi-Ershadi MR,Hasanzehi A,Shirzadi MR,Moosa-Kazemi SH,Sedaghat MM

    更新日期:2017-09-05 00:00:00

  • Enhancement of Pneumocandin B0 Production in Glarea lozoyensis by Low-Temperature Adaptive Laboratory Evolution.

    abstract::The production of pneumocandin B0 is limited by feedback inhibition. Here, low-temperature adaptive laboratory evolution (ALE) was used to improve the production capacity of Glarea lozoyensis by enhancing its membrane permeability. After 50 cycles of ALE, the pneumocandin B0 production of the endpoint strain (ALE50) r...

    journal_title:Frontiers in microbiology

    pub_type: 杂志文章

    doi:10.3389/fmicb.2018.02788

    authors: Song P,Zhang K,Zhang S,Huang BQ,Ji XJ,Ren LJ,Gao S,Wen JP,Huang H

    更新日期:2018-11-21 00:00:00