Abstract:
:Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infection mechanism for more rational composition of therapeutic cocktails. We present the study of two bacteriophages, infective for the Pectobacterium brasiliense strain F152. Podoviridae PP99 is a representative of the genus Zindervirus, and Myoviridae PP101 belongs to the still unclassified genomic group. The structure of O-polysaccharide of F152 was established by sugar analysis and 1D and 2D NMR spectroscopy: → 4)-α-D-Manp6Ac-(1→ 2)-α-D-Manp-(1→ 3)-β-D-Galp-(1→ 3 ↑ 1 α -l- 6 dTal p Ac 0 - 2 The recombinant tail spike protein of phage PP99, gp55, was shown to deacetylate the side chain talose residue of bacterial O-polysaccharide, thus providing the selective attachment of the phage to the cell surface. Both phages demonstrate lytic behavior, thus being prospective for therapeutic purposes.
journal_name
Front Microbioljournal_title
Frontiers in microbiologyauthors
Lukianova AA,Shneider MM,Evseev PV,Shpirt AM,Bugaeva EN,Kabanova AP,Obraztsova EA,Miroshnikov KK,Senchenkova SN,Shashkov AS,Toschakov SV,Knirel YA,Ignatov AN,Miroshnikov KAdoi
10.3389/fmicb.2019.03147subject
Has Abstractpub_date
2020-01-23 00:00:00pages
3147issn
1664-302Xjournal_volume
10pub_type
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