A peptide coating preventing the attachment of Porphyromonas gingivalis on the surfaces of dental implants.

Abstract:

OBJECTIVES:The aim of this study was to investigate whether a peptide-based coating can prevent the adhesion of Porphyromonas gingivalis, a key human pathogen associated with periodontitis and peri-implantitis. BACKGROUND:Nonsurgical and surgical interventions have been used for the treatment of peri-implantitis; however, the effectiveness of these approaches is usually unsatisfactory. The main reason is that dental plaque on the surface of the implant is difficult to remove due to its rough surface and thread design. Recently, a peptide-based coating for implant surfaces that can reject the adhesion of Escherichia coli and improve the attachment of host cells was developed. METHODS:A salivary pellicle was created on the surfaces of peptide-coated bare discs and verified with anti-human immunoglobulin G, A and M, and anti-fibrinogen. Early colonizers, Veillonella parvula and Streptococcus sobrinus, and the later colonizer, Porphyromonas gingivalis, were labelled with green and red fluorescent dyes, respectively, and seeded on the discs. Bacterial attachment was semi-quantified by fluorescence intensity. RESULTS:The salivary pellicle was evenly distributed on the discs, with or without the peptide coating, with an average thickness of 3.84 µm. A multi-species dental biofilm was created on the salivary pellicle. The peptide coating resulted in an approximate 25% reduction in the attachment of Veillonella parvula and Streptococcus sobrinus, and a 50% reduction in Porphyromonas gingivalis, when compared to control, uncoated implant discs. CONCLUSION:The novel peptide-based coating can inhibit the attachment of Porphyromonas gingivalis. It may have the potential to impede the development of peri-implantitis.

journal_name

J Periodontal Res

authors

Fang D,Yuran S,Reches M,Catunda R,Levin L,Febbraio M

doi

10.1111/jre.12737

subject

Has Abstract

pub_date

2020-08-01 00:00:00

pages

503-510

issue

4

eissn

0022-3484

issn

1600-0765

journal_volume

55

pub_type

杂志文章
  • Reactions of periodontal ligament epithelial cell clusters and OX6-immunopositive cells to experimental tooth movement and periodontitis.

    abstract:BACKGROUND AND OBJECTIVE:The aim of this study was to investigate reactions of periodontal ligament epithelial cell clusters and major histocompatibility complex class II (OX6)-immunopositive cells to simultaneously induced tooth movement and periodontitis employing Waldo's method. MATERIAL AND METHODS:Elastic gums we...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.2011.01376.x

    authors: Tadokoro O,Kawahara I,Vandevska-Radunovic V

    更新日期:2011-10-01 00:00:00

  • Leptotrichia buccalis hemagglutination in cell binding and salivary inhibition studies.

    abstract::The characteristic hemagglutination (HA) of Leptotrichia buccalis was used for measuring its attachment to various human cells and for determining if saliva contained hemagglutination inhibition (HI) factors. The microbial strain utilized displayed the characteristic EM morphology of L. buccalis. Sonicated preparation...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1978.tb00196.x

    authors: Falkler WA Jr,Hawley CE,Mongiello JR

    更新日期:1978-09-01 00:00:00

  • Long-term exposure of human gingival fibroblasts to cigarette smoke condensate reduces cell growth by modulating Bax, caspase-3 and p53 expression.

    abstract:BACKGROUND AND OBJECTIVE:Smoking cigarettes increases the risk of oral tissue damage leading to periodontal disease. Gingival fibroblasts, the predominant cell type inhabiting gingival connective tissue, play a critical role in remodeling and maintaining gingival structure. The objective of this study was to investigat...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12223

    authors: Alamri A,Semlali A,Jacques É,Alanazi M,Zakrzewski A,Chmielewski W,Rouabhia M

    更新日期:2015-08-01 00:00:00

  • Increased interleukin-1 beta (IL-1 beta) concentration in gingival tissue from periodontitis patients.

    abstract::Human gingival tissues from periodontitis patients were found to contain from 126 fg/mg to 2161 fg/mg interleukin-1 beta as determined by a sensitive enzyme linked immunoassay. No IL-1 beta could be found in normal gingival tissue. This finding may have important consequences relevant to connective tissue destruction ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1989.tb00883.x

    authors: Hönig J,Rordorf-Adam C,Siegmund C,Wiedemann W,Erard F

    更新日期:1989-11-01 00:00:00

  • Biochemical and histopathologic analysis of the effects of periodontitis on left ventricular heart tissues of rats.

    abstract:BACKGROUND AND OBJECTIVE:Current epidemiological works have suggested that chronic infections, such as periodontitis, are associated with an increased risk of cardiovascular diseases, including hypertrophy and heart failure. However, mechanisms behind the association are not known. The aim of this study was to evaluate...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12380

    authors: Köse O,Arabacı T,Gedikli S,Eminoglu DÖ,Kermen E,Kızıldağ A,Kara A,Ozkanlar S,Yemenoglu H

    更新日期:2017-04-01 00:00:00

  • Nicotine inhibits osteogenic differentiation of human periodontal ligament cells under cyclic tensile stress through canonical Wnt pathway and α7 nicotinic acetylcholine receptor.

    abstract:BACKGROUND AND OBJECTIVES:Nicotine, the main psychoactive component of tobacco, affects cell metabolism, proliferation, adhesion and, importantly, the osteogenic differentiation of fibroblasts. Approximately 15% of all orthodontic patients are adults among who one-fifth are smokers. Hence, it is necessary to have insig...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12545

    authors: Yu W,Hu B,Shi X,Cao Z,Ren M,He Z,Lin J,Deng H,Hu R

    更新日期:2018-08-01 00:00:00

  • Association analysis between the -2518MCP-1(A/G) polymorphism and generalized aggressive periodontitis in a Chinese population.

    abstract:BACKGROUND AND OBJECTIVE:It has been suggested that aggressive periodontitis has a genetic basis. Monocyte chemoattractant protein 1 (MCP-1) plays a critical role in the recruitment of monocytes and the development of periodontitis. The -2518MCP-1(A/G) polymorphism has been implicated as a risk or susceptibility factor...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.2011.01426.x

    authors: Zhu XL,Meng HX,Zhang L,Xu L,Chen ZB,Shi D,Feng XH,Zhang X

    更新日期:2012-06-01 00:00:00

  • An orally administered oral pathobiont and commensal have comparable and innocuous systemic effects in germ-free mice.

    abstract:BACKGROUND AND OBJECTIVES:We recently proposed a novel mechanism linking periodontitis and systemic diseases, in which orally administered Porphyromonas gingivalis affects gut microbiota composition and subsequently leads to systemic inflammation. However, the mechanism by which P. gingivalis generates systemic effects...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12593

    authors: Sato K,Yokoji M,Yamada M,Nakajima T,Yamazaki K

    更新日期:2018-12-01 00:00:00

  • Expression and intracellular localization of progesterone receptors in cultured human gingival fibroblasts.

    abstract:OBJECTIVE:The aim of this immunocytochemical study was to characterize the expression and distribution of the progesterone receptor (PR) and estrogen receptor (ER) in gingival fibroblasts using culture cells derived from people at various ages. BACKGROUND:The reaction of female hormones is tissue or cell specific, and...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1034/j.1600-0765.2003.00654.x

    authors: Kawahara K,Shimazu A

    更新日期:2003-06-01 00:00:00

  • Fibre guiding scaffolds for periodontal tissue engineering.

    abstract::The periodontium is a highly hierarchically organized organ composed of gingiva, alveolar bone, periodontal ligament and cementum. Periodontitis leads to the destruction of hard and soft tissues ultimately leading to a loss of the teeth supporting apparatus. Current treatments are capable of limiting the disease progr...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,评审

    doi:10.1111/jre.12729

    authors: Staples RJ,Ivanovski S,Vaquette C

    更新日期:2020-06-01 00:00:00

  • Five parameters of gingival crevicular fluid from eight surfaces in periodontal health and disease.

    abstract::Volume and amounts of myeloperoxidase (MPO), lactoferrin (LF), aryl sulfatase (AS) and lactate dehydrogenase (LDH) were measured in gingival crevicular fluid (GCF) collected from the mesial and distal proximal surfaces of the premolars and first and second molars of 3 subject groups. Group assignment was based on subj...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1992.tb01819.x

    authors: Smith QT,Au GS,Freese PL,Osborn JB,Stoltenberg JL

    更新日期:1992-09-01 00:00:00

  • Activated KCNQ1 channel promotes fibrogenic response in hereditary gingival fibromatosis via clustering and activation of Ras.

    abstract:BACKGROUND AND OBJECTIVE:Activated potassium channels were found to be strongly correlated with gingival overgrowth (GO) phenotype as we reviewed syndromic hereditary gingival fibromatosis (HGF). Nevertheless, the functional roles of potassium channels in gingival fibrosis or gingival overgrowth remained uncovered. The...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12836

    authors: Gao Q,Yang C,Meng L,Wang Z,Chen D,Peng Y,Yang K,Bian Z

    更新日期:2020-12-31 00:00:00

  • Localization of interleukin-1 (IL-1) mRNA-expressing macrophages in human inflamed gingiva and IL-1 activity in gingival crevicular fluid.

    abstract::The exact cell type and site(s) involved in interleukin-1 (IL-1) production during gingival inflammation was determined by combining immunohistochemistry and in situ hybridization. IL-1 messenger RNA (mRNA)-expressing cells in human inflamed gingiva were identified as macrophages. The rate of IL-alpha mRNA expression ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1993.tb01048.x

    authors: Matsuki Y,Yamamoto T,Hara K

    更新日期:1993-01-01 00:00:00

  • Prepubertal periodontitis: a review of diagnostic criteria, pathogenesis, and differential diagnosis.

    abstract::This paper reviews the literature on prepubertal periodontitis (PP) according to the definition and the classification of PP by Page. The generalized form of PP (G-PP) defined by Page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (LAD) and thus the pathogenesis is ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,评审

    doi:10.1111/j.1600-0765.1990.tb01205.x

    authors: Watanabe K

    更新日期:1990-01-01 00:00:00

  • Naringenin inhibits human osteoclastogenesis and osteoclastic bone resorption.

    abstract:BACKGROUND AND OBJECTIVE:Naringenin, a naturally occurring flavonoid, possesses a wide range of pharmacological properties. The purpose of this study was to investigate the effect of naringenin on human osteoclastogenesis and osteoclastic bone resorption. MATERIAL AND METHODS:Naringenin was tested in a human osteoclas...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.2008.01107.x

    authors: La VD,Tanabe S,Grenier D

    更新日期:2009-04-01 00:00:00

  • Alendronate regulates cytokine production induced by lipid A through nuclear factor-κB and Smad3 activation in human gingival fibroblasts.

    abstract:BACKGROUND AND OBJECTIVE:Nitrogen-containing bisphosphonates (NBPs) are widely used as anti-bone-resorptive drugs. However, use of NBPs results in inflammatory side-effects, including jaw osteomyelitis. In the present study, we examined the effects of alendronate, a typical NBP, on cytokine production by human peripher...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.2010.01302.x

    authors: Tamai R,Sugiyama A,Kiyoura Y

    更新日期:2011-02-01 00:00:00

  • Cost-effective design for dental randomized clinical trials with longitudinal observations.

    abstract::In general, randomized clinical trials (RCT) in dentistry involve longitudinal observations. In such studies, the total cost is a function of the number of study subjects and visits, the study duration, and the type and number of examinations at each visit. In this paper, we derived the minimum cost design for longitu...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1999.tb02233.x

    authors: Hefti AF,Yang MC

    更新日期:1999-04-01 00:00:00

  • Effects of TNFalpha and prostaglandin E2 on the expression of MMPs in human periodontal ligament fibroblasts.

    abstract::Accumulating evidence indicates that TNFalpha plays an important role in the pathogenesis of periodontitis, but the effect of TNFalpha on the degradation of the periodontal ligament is not well understood. This study used reverse transcriptase-PCR to investigate the effects of TNFalpha on matrix metalloproteinase (MMP...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1034/j.1600-0765.2002.00656.x

    authors: Nishikawa M,Yamaguchi Y,Yoshitake K,Saeki Y

    更新日期:2002-06-01 00:00:00

  • Oxidative burst intensity of peripheral phagocytic cells and periodontitis in Down syndrome.

    abstract:BACKGROUND:This study investigated the oxidative burst function of peripheral phagocytic cells (granulocytes and monocytes) and assessed the relation between oxidative burst and periodontal status in adult individuals with Down syndrome (DS) vs. other groups. METHODS:Of 55 DS individuals (18-56 years old), 74 individu...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12075

    authors: Khocht A,Russell B,Cannon JG,Turner B,Janal M

    更新日期:2014-02-01 00:00:00

  • Lipoprotein modifications by gingipains of Porphyromonas gingivalis.

    abstract:BACKGROUND AND OBJECTIVE:Several studies have shown an association between periodontitis and cardiovascular disease (CVD). Atherosclerosis is the major cause of CVD, and a key event in the development of atherosclerosis is accumulation of lipoproteins within the arterial wall. Bacteria are the primary etiologic agents ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12527

    authors: Lönn J,Ljunggren S,Klarström-Engström K,Demirel I,Bengtsson T,Karlsson H

    更新日期:2018-06-01 00:00:00

  • Production of trypsin and chymotrypsin inhibitors by oral bacterial isolates.

    abstract::Using an agar-skim milk gel method, over 300 oral bacterial isolates were tested for their ability to produce trypsin or chymotrypsin inhibitor. Eleven bacterial strains, including seven isolates identified as Bacteroides intermedius, were found to inhibit both proteolytic activities. The inhibitory factors of the 11 ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1991.tb01726.x

    authors: Grenier D

    更新日期:1991-09-01 00:00:00

  • Long junctional epithelium produced by application of bacterial protease in rats.

    abstract::In the present study, the effect of bacterial protease on the periodontal tissues in rats was investigated histologically and histometrically. In the control group, 0.03 ml distilled water was applied to the lingual marginal gingiva of the mandibular incisor while, in the experimental group, 0.03 ml bacterial protease...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1989.tb02009.x

    authors: Suzumura Y,Kameyama Y,Mizutani M,Kato M,Kondo K,Mabuchi R

    更新日期:1989-05-01 00:00:00

  • Evidence that cyclosporine inhibits periodontal prostaglandin I2 synthesis.

    abstract::Cyclosporine (CsA) is a selective immunosuppressant widely used in clinical therapy. Like phenytoin and nifedipine, the drug is associated with gingival overgrowth. This study considers the interaction of CsA and prostaglandin I2 (PGI2), in particular the action of the drug on gingival tissue in vitro and in vivo. The...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1996.tb00474.x

    authors: Nell A,Matejka M,Solar P,Ulm C,Sinzinger H

    更新日期:1996-02-01 00:00:00

  • Cystatin A in gingival crevicular fluid of periodontal patients.

    abstract::Cystatins are physiological inhibitors of cysteine proteinases which are widely distributed in human tissues and fluids. In the present study we analysed both the cystatin activity and the different cystatin isoforms in gingival crevicular fluid and saliva samples of nine periodontitis patients. All crevicular fluid s...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1997.tb00935.x

    authors: Blankenvoorde MF,Henskens YM,van der Weijden GA,van den Keijbus PA,Veerman EC,Nieuw Amerongen AV

    更新日期:1997-10-01 00:00:00

  • Use of water 'softening and conditioning systems' significantly increases the risk of periodontitis: smoking considerations.

    abstract:BACKGROUND:The role of water in the etiology of periodontal disease is poorly understood. OBJECTIVES:The objective of this study was to examine the association amongst water softness, risk for periodontitis, and smoking status. METHODS:We examined the association between use of water 'softening and conditioning syste...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.2004.00769.x

    authors: Wood N,Johnson RB

    更新日期:2004-12-01 00:00:00

  • Phagocytosis of Porphyromonas gingivalis and Prevotella intermedia by human polymorphonuclear leukocytes in clots formed from human plasma or purified fibrinogen.

    abstract::We have studied the polymorphonuclear leukocyte (PMN) phagocytosis of Porphyromonas gingivalis (Bacteroides gingivalis) and Prevotella intermedia (Bacteroides intermedius) in three-dimensional fibrin meshworks formed by either clotting human plasma or by clotting purified fibrinogen with human serum incorporated. PMN ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1992.tb01812.x

    authors: Hurst TJ,Wilton JM

    更新日期:1992-03-01 00:00:00

  • Platelet-derived and insulin-like growth factors stimulate regeneration of periodontal attachment in monkeys.

    abstract::Regeneration of cementum, ligament, bone and new attachment was achieved by introducing mixtures of recombinant human platelet-derived growth factor and insulin-like growth factor into debrided lesions of experimentally induced periodontitis in monkeys. This growth factor therapeutic regimen induced the regeneration o...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.1992.tb01679.x

    authors: Rutherford RB,Niekrash CE,Kennedy JE,Charette MF

    更新日期:1992-07-01 00:00:00

  • Defense mechanism of heme oxygenase-1 against cytotoxic and receptor activator of nuclear factor-kappaB ligand inducing effects of hydrogen peroxide in human periodontal ligament cells.

    abstract:BACKGROUND AND OBJECTIVE:Although induction of heme oxygenase-1 by H2O2 has been reported, the protective role of heme oxygenase-1 against the cytotoxic and osteoclastogenic effects of H2O2 have not been elucidated in human periodontal ligament cells. The aim of this work was to investigate the defense mechanism of hem...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.2006.00953.x

    authors: Pi SH,Kim SC,Kim HT,Lee HJ,Lee SK,Kim EC

    更新日期:2007-08-01 00:00:00

  • Periodontal disease, tooth loss and coronary heart disease assessed by coronary angiography: a cross-sectional observational study.

    abstract:OBJECTIVE AND BACKGROUND:To evaluate the association between periodontal disease, tooth loss and coronary heart disease (CHD). There is still controversy about the relationship between periodontal disease and tooth loss with vessel obstruction assessed using coronary angiography. MATERIAL AND METHODS:This cross-sectio...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/jre.12301

    authors: Zanella SM,Pereira SS,Barbisan JN,Vieira L,Saba-Chujfi E,Haas AN,Rösing CK

    更新日期:2016-04-01 00:00:00

  • Serum cytokine levels and periodontal parameters in ankylosing spondylitis.

    abstract:BACKGROUND AND OBJECTIVE:Multiple studies support the role of periodontal disease in contributing to the chronic systemic inflammatory burden in a variety of diseases, including ankylosing spondylitis (AS), in the progression which the inflammatory process plays an important role. We assume that patients with AS are mo...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章

    doi:10.1111/j.1600-0765.2011.01448.x

    authors: Sezer U,Erciyas K,Pehlivan Y,Ustün K,Tarakçioğlu M,Senyurt SZ,Onat AM

    更新日期:2012-06-01 00:00:00