Monitoring abscopal responses to radiation in mice.

Abstract:

:Focal radiation therapy has the potential to generate systemic tumor-targeting immune responses so potent as to eradicate anatomically distant, non-irradiated malignant lesions, a phenomenon commonly referred to as "the abscopal response." In cancer patients, bona fide abscopal responses are rare, although the recent introduction of immune checkpoint blockers into the clinical practice has significantly increased their incidence. In rodents, abscopal responses can be conveniently modeled by establishing two, slightly asynchronous and anatomically distant subcutaneous tumors in syngeneic immunocompetent hosts, provided that the therapeutic partners of radiation potentially included in the regimen of choice do not mediate systemic anticancer effects per se. Here, we describe such method to monitor abscopal responses based on mammary carcinoma TSA cells implanted in syngeneic immunocompetent BALB/c mice. With minor variations, the same technique can be conveniently applied to a variety of transplantable mouse tumors.

journal_name

Methods Enzymol

journal_title

Methods in enzymology

authors

Rodriguez-Ruiz ME,Yamazaki T,Buqué A,Bloy N,Silva VAO,Stafford L,Sato A,Galluzzi L

doi

10.1016/bs.mie.2019.04.014

subject

Has Abstract

pub_date

2020-01-01 00:00:00

pages

111-125

eissn

0076-6879

issn

1557-7988

pii

S0076-6879(19)30129-6

journal_volume

635

pub_type

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